Genetic Diversity Loss in Chironomus sancticaroli (Diptera: Chironomidae) Exposed to Pyrimethanil Fungicide: an Analysis Using RAPD Technique

Detalhes bibliográficos
Autor(a) principal: Colombo-Corbi, Vanessa
Data de Publicação: 2017
Outros Autores: Gorni, Guilherme Rossi, Sanzovo-Falcoski, Thais [UNESP], Costa, Paulo Inácio [UNESP], Corbi, Juliano J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s11270-017-3578-z
http://hdl.handle.net/11449/170198
Resumo: In the agricultural cultivation, the Pyrimethanil (4,6-dimethyl-N-phenyl-2-pyrimidinamine) fungicide is one of the most widely used compound in monocultures and has been detected in aquatic ecosystems. These genotoxic products increase the frequency of lesions in the DNA molecule, thereby increasing the risk of replication and transcription of altered DNA sequences. This study aimed to assess the loss of genetic diversity of Chironomus sancticaroli, a Brazilian native insect species, exposed to Pyrimethanil fungicide, using RAPD-PCR (random amplified polymorphic DNA) technique. The results showed that there was significant loss in the genetic diversity in the organisms exposed to high Pyrimethanil concentrations when compared with control site. Our findings reveal that RAPD-PCR is an effective method to access genetic loss derived to fungicide use and that the agriculture application may lead to a decrease in aquatic biota genetic diversity. This finding has important implications for conservation strategies and ecological management environments.
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spelling Genetic Diversity Loss in Chironomus sancticaroli (Diptera: Chironomidae) Exposed to Pyrimethanil Fungicide: an Analysis Using RAPD TechniqueAgriculture cultivationChironomidaeGenetic diversityStreamIn the agricultural cultivation, the Pyrimethanil (4,6-dimethyl-N-phenyl-2-pyrimidinamine) fungicide is one of the most widely used compound in monocultures and has been detected in aquatic ecosystems. These genotoxic products increase the frequency of lesions in the DNA molecule, thereby increasing the risk of replication and transcription of altered DNA sequences. This study aimed to assess the loss of genetic diversity of Chironomus sancticaroli, a Brazilian native insect species, exposed to Pyrimethanil fungicide, using RAPD-PCR (random amplified polymorphic DNA) technique. The results showed that there was significant loss in the genetic diversity in the organisms exposed to high Pyrimethanil concentrations when compared with control site. Our findings reveal that RAPD-PCR is an effective method to access genetic loss derived to fungicide use and that the agriculture application may lead to a decrease in aquatic biota genetic diversity. This finding has important implications for conservation strategies and ecological management environments.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)PPG Desenvolvimento Territorial e Meio Ambiente – Universidade de Araraquara UNIARAFaculdade de Ciências Farmacêuticas de Araraquara Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESPDepartamento de Hidráulica e Saneamento Escola de Engenharia de São Carlos (EESC) Universidade de São Paulo – USP, C.P. 359Faculdade de Ciências Farmacêuticas de Araraquara Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESPUNIARAUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Colombo-Corbi, VanessaGorni, Guilherme RossiSanzovo-Falcoski, Thais [UNESP]Costa, Paulo Inácio [UNESP]Corbi, Juliano J.2018-12-11T16:49:43Z2018-12-11T16:49:43Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1007/s11270-017-3578-zWater, Air, and Soil Pollution, v. 228, n. 10, 2017.1573-29320049-6979http://hdl.handle.net/11449/17019810.1007/s11270-017-3578-z2-s2.0-850298490042-s2.0-85029849004.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengWater, Air, and Soil Pollution0,5890,589info:eu-repo/semantics/openAccess2023-12-12T06:17:27Zoai:repositorio.unesp.br:11449/170198Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:06:40.445150Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genetic Diversity Loss in Chironomus sancticaroli (Diptera: Chironomidae) Exposed to Pyrimethanil Fungicide: an Analysis Using RAPD Technique
title Genetic Diversity Loss in Chironomus sancticaroli (Diptera: Chironomidae) Exposed to Pyrimethanil Fungicide: an Analysis Using RAPD Technique
spellingShingle Genetic Diversity Loss in Chironomus sancticaroli (Diptera: Chironomidae) Exposed to Pyrimethanil Fungicide: an Analysis Using RAPD Technique
Colombo-Corbi, Vanessa
Agriculture cultivation
Chironomidae
Genetic diversity
Stream
title_short Genetic Diversity Loss in Chironomus sancticaroli (Diptera: Chironomidae) Exposed to Pyrimethanil Fungicide: an Analysis Using RAPD Technique
title_full Genetic Diversity Loss in Chironomus sancticaroli (Diptera: Chironomidae) Exposed to Pyrimethanil Fungicide: an Analysis Using RAPD Technique
title_fullStr Genetic Diversity Loss in Chironomus sancticaroli (Diptera: Chironomidae) Exposed to Pyrimethanil Fungicide: an Analysis Using RAPD Technique
title_full_unstemmed Genetic Diversity Loss in Chironomus sancticaroli (Diptera: Chironomidae) Exposed to Pyrimethanil Fungicide: an Analysis Using RAPD Technique
title_sort Genetic Diversity Loss in Chironomus sancticaroli (Diptera: Chironomidae) Exposed to Pyrimethanil Fungicide: an Analysis Using RAPD Technique
author Colombo-Corbi, Vanessa
author_facet Colombo-Corbi, Vanessa
Gorni, Guilherme Rossi
Sanzovo-Falcoski, Thais [UNESP]
Costa, Paulo Inácio [UNESP]
Corbi, Juliano J.
author_role author
author2 Gorni, Guilherme Rossi
Sanzovo-Falcoski, Thais [UNESP]
Costa, Paulo Inácio [UNESP]
Corbi, Juliano J.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv UNIARA
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Colombo-Corbi, Vanessa
Gorni, Guilherme Rossi
Sanzovo-Falcoski, Thais [UNESP]
Costa, Paulo Inácio [UNESP]
Corbi, Juliano J.
dc.subject.por.fl_str_mv Agriculture cultivation
Chironomidae
Genetic diversity
Stream
topic Agriculture cultivation
Chironomidae
Genetic diversity
Stream
description In the agricultural cultivation, the Pyrimethanil (4,6-dimethyl-N-phenyl-2-pyrimidinamine) fungicide is one of the most widely used compound in monocultures and has been detected in aquatic ecosystems. These genotoxic products increase the frequency of lesions in the DNA molecule, thereby increasing the risk of replication and transcription of altered DNA sequences. This study aimed to assess the loss of genetic diversity of Chironomus sancticaroli, a Brazilian native insect species, exposed to Pyrimethanil fungicide, using RAPD-PCR (random amplified polymorphic DNA) technique. The results showed that there was significant loss in the genetic diversity in the organisms exposed to high Pyrimethanil concentrations when compared with control site. Our findings reveal that RAPD-PCR is an effective method to access genetic loss derived to fungicide use and that the agriculture application may lead to a decrease in aquatic biota genetic diversity. This finding has important implications for conservation strategies and ecological management environments.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
2018-12-11T16:49:43Z
2018-12-11T16:49:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s11270-017-3578-z
Water, Air, and Soil Pollution, v. 228, n. 10, 2017.
1573-2932
0049-6979
http://hdl.handle.net/11449/170198
10.1007/s11270-017-3578-z
2-s2.0-85029849004
2-s2.0-85029849004.pdf
url http://dx.doi.org/10.1007/s11270-017-3578-z
http://hdl.handle.net/11449/170198
identifier_str_mv Water, Air, and Soil Pollution, v. 228, n. 10, 2017.
1573-2932
0049-6979
10.1007/s11270-017-3578-z
2-s2.0-85029849004
2-s2.0-85029849004.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Water, Air, and Soil Pollution
0,589
0,589
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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