Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11032-016-0570-z http://hdl.handle.net/11449/173773 |
Resumo: | Water deficit may occur at any stage of crop development, affecting productivity and causing economic losses. In response to drought, raffinose family oligosaccharides (RFOs) are accumulated in plant tissues stabilizing and protecting cell membranes and keeping the vital functions. The enzyme galactinol synthase (GolS, EC 2.4.1.123) catalyzes the first step in the biosynthesis of RFOs. In our study, soybean events overexpressing 35S:AtGolS2 were molecularly, physiological, and agronomical characterized, under drought simulated in greenhouse and in field conditions during the crop season 2014/2015. The conventional soybean cultivar BRS 184 was transformed and five positive events were obtained. Four events transmitted the transgene to further generations and in the events 2Ia1 and 2Ia4, two to four copies of AtGols2 gene were observed. Results in greenhouse showed that the overexpression of AtGolS2 in genetically modified (GM) plants led to increased galactinol transcripts, probably resulting in changes in carbohydrate metabolism. Accumulation of these transcripts that may have acted as osmoprotectors, lead to higher drought tolerance and survival rate of 2Ia4 plants. In addition, in field conditions, higher yield was observed for 2Ia4 plants under irrigated (IRR) and non-irrigated (NIRR) treatments. This result can be due to the increased synthesis of RFOs even under well-watered conditions. This field screening showed promising results for drought tolerance, suggesting that 2Ia4 plants may be useful in a breeding program for the development of drought-tolerant plants. However, additional studies are needed in further crop seasons and other sites to better characterize how these plants may outperform the WT plants under water deficit. |
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Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought toleranceAbiotic stressesGalactinol synthaseGlycine max L. MerrillRaffinoseWater deficit may occur at any stage of crop development, affecting productivity and causing economic losses. In response to drought, raffinose family oligosaccharides (RFOs) are accumulated in plant tissues stabilizing and protecting cell membranes and keeping the vital functions. The enzyme galactinol synthase (GolS, EC 2.4.1.123) catalyzes the first step in the biosynthesis of RFOs. In our study, soybean events overexpressing 35S:AtGolS2 were molecularly, physiological, and agronomical characterized, under drought simulated in greenhouse and in field conditions during the crop season 2014/2015. The conventional soybean cultivar BRS 184 was transformed and five positive events were obtained. Four events transmitted the transgene to further generations and in the events 2Ia1 and 2Ia4, two to four copies of AtGols2 gene were observed. Results in greenhouse showed that the overexpression of AtGolS2 in genetically modified (GM) plants led to increased galactinol transcripts, probably resulting in changes in carbohydrate metabolism. Accumulation of these transcripts that may have acted as osmoprotectors, lead to higher drought tolerance and survival rate of 2Ia4 plants. In addition, in field conditions, higher yield was observed for 2Ia4 plants under irrigated (IRR) and non-irrigated (NIRR) treatments. This result can be due to the increased synthesis of RFOs even under well-watered conditions. This field screening showed promising results for drought tolerance, suggesting that 2Ia4 plants may be useful in a breeding program for the development of drought-tolerant plants. However, additional studies are needed in further crop seasons and other sites to better characterize how these plants may outperform the WT plants under water deficit.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Applied Biology to Farming FCAV-UNESPEmbrapa Soybean Rodovia Carlos João Strass Acesso Orlando Amaral Warta Coordination for the Improvement of Higher Education Personnel (CAPES), PO. Box 231 86001-970Embrapa Soybean Rodovia Carlos João Strass Acesso Orlando Amaral Warta National Council for Scientific and Technological Development (CNPq), PO. Box 231 86001-970Embrapa Soybean Rodovia Carlos João Strass Acesso Orlando Amaral Warta, PO. Box 231 86001-970Department of General Biology Londrina State UniversityBiological Resources and Post-harvest Division Japan International Research Center for Agricultural SciencesGene Discovery Research Group RIKEN Center for Sustainable Resource ScienceLaboratory of Plant Molecular Physiology The University of Tokyo, Bunkyo-kuDepartment of Applied Biology to Farming FCAV-UNESPUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual de Londrina (UEL)Japan International Research Center for Agricultural SciencesRIKEN Center for Sustainable Resource ScienceThe University of TokyoHonna, Patricia T. [UNESP]Fuganti-Pagliarini, RenataFerreira, Leonardo C.Molinari, Mayla D. C.Marin, Silvana R. R.de Oliveira, Maria C. N.Farias, José R. B.Neumaier, NormanMertz-Henning, Liliane M.Kanamori, NorihitoNakashima, KazuoTakasaki, HironoriUrano, KaoruShinozaki, KazuoYamaguchi-Shinozaki, KazukoDesidério, Janete A. [UNESP]Nepomuceno, Alexandre L.2018-12-11T17:07:40Z2018-12-11T17:07:40Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1007/s11032-016-0570-zMolecular Breeding, v. 36, n. 11, 2016.1572-97881380-3743http://hdl.handle.net/11449/17377310.1007/s11032-016-0570-z2-s2.0-849947503912-s2.0-84994750391.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular Breeding1,1391,139info:eu-repo/semantics/openAccess2023-10-19T06:09:14Zoai:repositorio.unesp.br:11449/173773Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-19T06:09:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance |
title |
Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance |
spellingShingle |
Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance Honna, Patricia T. [UNESP] Abiotic stresses Galactinol synthase Glycine max L. Merrill Raffinose |
title_short |
Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance |
title_full |
Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance |
title_fullStr |
Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance |
title_full_unstemmed |
Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance |
title_sort |
Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance |
author |
Honna, Patricia T. [UNESP] |
author_facet |
Honna, Patricia T. [UNESP] Fuganti-Pagliarini, Renata Ferreira, Leonardo C. Molinari, Mayla D. C. Marin, Silvana R. R. de Oliveira, Maria C. N. Farias, José R. B. Neumaier, Norman Mertz-Henning, Liliane M. Kanamori, Norihito Nakashima, Kazuo Takasaki, Hironori Urano, Kaoru Shinozaki, Kazuo Yamaguchi-Shinozaki, Kazuko Desidério, Janete A. [UNESP] Nepomuceno, Alexandre L. |
author_role |
author |
author2 |
Fuganti-Pagliarini, Renata Ferreira, Leonardo C. Molinari, Mayla D. C. Marin, Silvana R. R. de Oliveira, Maria C. N. Farias, José R. B. Neumaier, Norman Mertz-Henning, Liliane M. Kanamori, Norihito Nakashima, Kazuo Takasaki, Hironori Urano, Kaoru Shinozaki, Kazuo Yamaguchi-Shinozaki, Kazuko Desidério, Janete A. [UNESP] Nepomuceno, Alexandre L. |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual de Londrina (UEL) Japan International Research Center for Agricultural Sciences RIKEN Center for Sustainable Resource Science The University of Tokyo |
dc.contributor.author.fl_str_mv |
Honna, Patricia T. [UNESP] Fuganti-Pagliarini, Renata Ferreira, Leonardo C. Molinari, Mayla D. C. Marin, Silvana R. R. de Oliveira, Maria C. N. Farias, José R. B. Neumaier, Norman Mertz-Henning, Liliane M. Kanamori, Norihito Nakashima, Kazuo Takasaki, Hironori Urano, Kaoru Shinozaki, Kazuo Yamaguchi-Shinozaki, Kazuko Desidério, Janete A. [UNESP] Nepomuceno, Alexandre L. |
dc.subject.por.fl_str_mv |
Abiotic stresses Galactinol synthase Glycine max L. Merrill Raffinose |
topic |
Abiotic stresses Galactinol synthase Glycine max L. Merrill Raffinose |
description |
Water deficit may occur at any stage of crop development, affecting productivity and causing economic losses. In response to drought, raffinose family oligosaccharides (RFOs) are accumulated in plant tissues stabilizing and protecting cell membranes and keeping the vital functions. The enzyme galactinol synthase (GolS, EC 2.4.1.123) catalyzes the first step in the biosynthesis of RFOs. In our study, soybean events overexpressing 35S:AtGolS2 were molecularly, physiological, and agronomical characterized, under drought simulated in greenhouse and in field conditions during the crop season 2014/2015. The conventional soybean cultivar BRS 184 was transformed and five positive events were obtained. Four events transmitted the transgene to further generations and in the events 2Ia1 and 2Ia4, two to four copies of AtGols2 gene were observed. Results in greenhouse showed that the overexpression of AtGolS2 in genetically modified (GM) plants led to increased galactinol transcripts, probably resulting in changes in carbohydrate metabolism. Accumulation of these transcripts that may have acted as osmoprotectors, lead to higher drought tolerance and survival rate of 2Ia4 plants. In addition, in field conditions, higher yield was observed for 2Ia4 plants under irrigated (IRR) and non-irrigated (NIRR) treatments. This result can be due to the increased synthesis of RFOs even under well-watered conditions. This field screening showed promising results for drought tolerance, suggesting that 2Ia4 plants may be useful in a breeding program for the development of drought-tolerant plants. However, additional studies are needed in further crop seasons and other sites to better characterize how these plants may outperform the WT plants under water deficit. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 2018-12-11T17:07:40Z 2018-12-11T17:07:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11032-016-0570-z Molecular Breeding, v. 36, n. 11, 2016. 1572-9788 1380-3743 http://hdl.handle.net/11449/173773 10.1007/s11032-016-0570-z 2-s2.0-84994750391 2-s2.0-84994750391.pdf |
url |
http://dx.doi.org/10.1007/s11032-016-0570-z http://hdl.handle.net/11449/173773 |
identifier_str_mv |
Molecular Breeding, v. 36, n. 11, 2016. 1572-9788 1380-3743 10.1007/s11032-016-0570-z 2-s2.0-84994750391 2-s2.0-84994750391.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecular Breeding 1,139 1,139 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964623713075200 |