Antibacterial activity, cytocompatibility and effect of Bio-C Temp bioceramic intracanal medicament on osteoblast biology
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/iej.13502 http://hdl.handle.net/11449/207466 |
Resumo: | Aim: To analyse the antimicrobial and biological properties of a new bioceramic intracanal medicament (Bio-C Temp), and to compare it with two calcium hydroxide-based intracanal medicaments (Calen® and UltraCal® XS). Methodology: The direct contact and the crystal violet tests were performed to assess the antimicrobial activity of intracanal medicaments against Enterococcus faecalis. The cytocompatibility and the effect of the medication on the biology of the human osteoblast-like cell line (Saos-2) were evaluated with methylthiazole tetrazolium (MTT), neutral red, alkaline phosphatase activity and mineralization (alizarin red) assays. The data were analysed using one-way anova and Tukey’s tests, two-way anova and Bonferroni’s tests, or Kruskal–Wallis and Dunn’s tests (α = 0.05). Results: Bio-C Temp had significantly less antibacterial activity and biofilm biomass reduction than the other intracanal medicaments (P < 0.05). There was no difference in the viability of Saos-2 exposed to the various intracanal medicaments, except regarding the 1 : 2 dilution, when the Bio-C Temp group had significantly lower cell viability than the UltraCal® XS and Calen® groups (P < 0.05). Bio-C Temp induced significantly greater ALP activity than the other intracanal medicaments (P < 0.05) at day 1. Calen® induced significantly greater deposition of mineralized nodules than the other intracanal medicaments (P < 0.05), and no difference was observed between Bio-C Temp and UltraCal® XS (P > 0.05). Conclusions: Bio-C Temp had similar cytocompatibility at higher dilutions, and higher or similar induction of ALP activity and deposition of mineralized nodules in comparison with Calen® and UltraCal® XS. However, it had significantly less antibacterial and antibiofilm activity than Calen® and UltraCal® XS. |
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Repositório Institucional da UNESP |
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2946 |
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Antibacterial activity, cytocompatibility and effect of Bio-C Temp bioceramic intracanal medicament on osteoblast biologybioceramicscytotoxicityEnterococcus faecalisintracanal medicationAim: To analyse the antimicrobial and biological properties of a new bioceramic intracanal medicament (Bio-C Temp), and to compare it with two calcium hydroxide-based intracanal medicaments (Calen® and UltraCal® XS). Methodology: The direct contact and the crystal violet tests were performed to assess the antimicrobial activity of intracanal medicaments against Enterococcus faecalis. The cytocompatibility and the effect of the medication on the biology of the human osteoblast-like cell line (Saos-2) were evaluated with methylthiazole tetrazolium (MTT), neutral red, alkaline phosphatase activity and mineralization (alizarin red) assays. The data were analysed using one-way anova and Tukey’s tests, two-way anova and Bonferroni’s tests, or Kruskal–Wallis and Dunn’s tests (α = 0.05). Results: Bio-C Temp had significantly less antibacterial activity and biofilm biomass reduction than the other intracanal medicaments (P < 0.05). There was no difference in the viability of Saos-2 exposed to the various intracanal medicaments, except regarding the 1 : 2 dilution, when the Bio-C Temp group had significantly lower cell viability than the UltraCal® XS and Calen® groups (P < 0.05). Bio-C Temp induced significantly greater ALP activity than the other intracanal medicaments (P < 0.05) at day 1. Calen® induced significantly greater deposition of mineralized nodules than the other intracanal medicaments (P < 0.05), and no difference was observed between Bio-C Temp and UltraCal® XS (P > 0.05). Conclusions: Bio-C Temp had similar cytocompatibility at higher dilutions, and higher or similar induction of ALP activity and deposition of mineralized nodules in comparison with Calen® and UltraCal® XS. However, it had significantly less antibacterial and antibiofilm activity than Calen® and UltraCal® XS.Department of Restorative Dentistry Araraquara School of Dentistry UNESP - São Paulo State UniversityAraraquara School of Pharmaceutical Sciences UNESP - São Paulo State UniversityDepartment of Restorative Dentistry Araraquara School of Dentistry UNESP - São Paulo State UniversityAraraquara School of Pharmaceutical Sciences UNESP - São Paulo State UniversityUniversidade Estadual Paulista (Unesp)Guerreiro, J. C.M. [UNESP]Ochoa-Rodrígez, V. M. [UNESP]Rodrigues, E. M. [UNESP]Chavez-Andrade, G. M. [UNESP]Tanomaru-Filho, M. [UNESP]Guerreiro-Tanomaru, J. M. [UNESP]Faria, G. [UNESP]2021-06-25T10:55:41Z2021-06-25T10:55:41Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/iej.13502International Endodontic Journal.1365-25910143-2885http://hdl.handle.net/11449/20746610.1111/iej.135022-s2.0-85102647099Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Endodontic Journalinfo:eu-repo/semantics/openAccess2021-10-23T17:16:59Zoai:repositorio.unesp.br:11449/207466Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T17:16:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antibacterial activity, cytocompatibility and effect of Bio-C Temp bioceramic intracanal medicament on osteoblast biology |
title |
Antibacterial activity, cytocompatibility and effect of Bio-C Temp bioceramic intracanal medicament on osteoblast biology |
spellingShingle |
Antibacterial activity, cytocompatibility and effect of Bio-C Temp bioceramic intracanal medicament on osteoblast biology Guerreiro, J. C.M. [UNESP] bioceramics cytotoxicity Enterococcus faecalis intracanal medication |
title_short |
Antibacterial activity, cytocompatibility and effect of Bio-C Temp bioceramic intracanal medicament on osteoblast biology |
title_full |
Antibacterial activity, cytocompatibility and effect of Bio-C Temp bioceramic intracanal medicament on osteoblast biology |
title_fullStr |
Antibacterial activity, cytocompatibility and effect of Bio-C Temp bioceramic intracanal medicament on osteoblast biology |
title_full_unstemmed |
Antibacterial activity, cytocompatibility and effect of Bio-C Temp bioceramic intracanal medicament on osteoblast biology |
title_sort |
Antibacterial activity, cytocompatibility and effect of Bio-C Temp bioceramic intracanal medicament on osteoblast biology |
author |
Guerreiro, J. C.M. [UNESP] |
author_facet |
Guerreiro, J. C.M. [UNESP] Ochoa-Rodrígez, V. M. [UNESP] Rodrigues, E. M. [UNESP] Chavez-Andrade, G. M. [UNESP] Tanomaru-Filho, M. [UNESP] Guerreiro-Tanomaru, J. M. [UNESP] Faria, G. [UNESP] |
author_role |
author |
author2 |
Ochoa-Rodrígez, V. M. [UNESP] Rodrigues, E. M. [UNESP] Chavez-Andrade, G. M. [UNESP] Tanomaru-Filho, M. [UNESP] Guerreiro-Tanomaru, J. M. [UNESP] Faria, G. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Guerreiro, J. C.M. [UNESP] Ochoa-Rodrígez, V. M. [UNESP] Rodrigues, E. M. [UNESP] Chavez-Andrade, G. M. [UNESP] Tanomaru-Filho, M. [UNESP] Guerreiro-Tanomaru, J. M. [UNESP] Faria, G. [UNESP] |
dc.subject.por.fl_str_mv |
bioceramics cytotoxicity Enterococcus faecalis intracanal medication |
topic |
bioceramics cytotoxicity Enterococcus faecalis intracanal medication |
description |
Aim: To analyse the antimicrobial and biological properties of a new bioceramic intracanal medicament (Bio-C Temp), and to compare it with two calcium hydroxide-based intracanal medicaments (Calen® and UltraCal® XS). Methodology: The direct contact and the crystal violet tests were performed to assess the antimicrobial activity of intracanal medicaments against Enterococcus faecalis. The cytocompatibility and the effect of the medication on the biology of the human osteoblast-like cell line (Saos-2) were evaluated with methylthiazole tetrazolium (MTT), neutral red, alkaline phosphatase activity and mineralization (alizarin red) assays. The data were analysed using one-way anova and Tukey’s tests, two-way anova and Bonferroni’s tests, or Kruskal–Wallis and Dunn’s tests (α = 0.05). Results: Bio-C Temp had significantly less antibacterial activity and biofilm biomass reduction than the other intracanal medicaments (P < 0.05). There was no difference in the viability of Saos-2 exposed to the various intracanal medicaments, except regarding the 1 : 2 dilution, when the Bio-C Temp group had significantly lower cell viability than the UltraCal® XS and Calen® groups (P < 0.05). Bio-C Temp induced significantly greater ALP activity than the other intracanal medicaments (P < 0.05) at day 1. Calen® induced significantly greater deposition of mineralized nodules than the other intracanal medicaments (P < 0.05), and no difference was observed between Bio-C Temp and UltraCal® XS (P > 0.05). Conclusions: Bio-C Temp had similar cytocompatibility at higher dilutions, and higher or similar induction of ALP activity and deposition of mineralized nodules in comparison with Calen® and UltraCal® XS. However, it had significantly less antibacterial and antibiofilm activity than Calen® and UltraCal® XS. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:55:41Z 2021-06-25T10:55:41Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/iej.13502 International Endodontic Journal. 1365-2591 0143-2885 http://hdl.handle.net/11449/207466 10.1111/iej.13502 2-s2.0-85102647099 |
url |
http://dx.doi.org/10.1111/iej.13502 http://hdl.handle.net/11449/207466 |
identifier_str_mv |
International Endodontic Journal. 1365-2591 0143-2885 10.1111/iej.13502 2-s2.0-85102647099 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Endodontic Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046625327710208 |