Molecular characterization of Anaplasma marginale in ticks naturally feeding on buffaloes
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.meegid.2015.07.027 http://hdl.handle.net/11449/158553 |
Resumo: | Anaplasma marginale is the most prevalent pathogen transmitted by ticks in cattle in tropical and subtropical regions of the world. However, the tick species involved in the transmission of A. marginale in buffaloes in Brazil have not been identified. The objective of the present study was to determine the presence of A. marginale in ticks parasitizing water buffaloes. A total of 200 samples of Rhipicephalus microplus, Dermacentor nitens, Amblyomma cajennense, and Amblyomma maculatum were collected and tested by conventional and quantitative PCR for the presence of the msp1a and msp5 genes. In the present study, 35 ticks (17.5%) were positive for A. marginale DNA by qPCR analysis. The positive ticks belonged to four different species: R. microplus (22.2%), A. cajennense (13.8%), A. maculatum (16.0%), and D. nitens (10.0%). Individuals of the three developmental stages (larvae, nymphs, and adults) of R. microplus and A. cajennense were found to be positive for A. marginale, only nymphs and adults of A. maculatum were found to be positive, and finally, only adults of D. nitens were positive for A. marginale. Our results suggest that R. microplus, A. cajennense, A. maculatum, and D. nitens ticks may be involved in the transmission of A. marg-inale in buffaloes. However, while A. marg-inale PCR positive ticks were recorded, this does not indicate vector competence; only that the ticks may contain a blood meal from an infected host. Additionally, the results show that the strains of A. marginale from buffaloes and cattle are phylogenetically related. (C) 2015 Elsevier B.V. All rights reserved. |
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Molecular characterization of Anaplasma marginale in ticks naturally feeding on buffaloesAmblyomma cajennenseAmblyomma maculatumAnaplasma marginaleDermacentor nitensRhipicephalus microplusAnaplasma marginale is the most prevalent pathogen transmitted by ticks in cattle in tropical and subtropical regions of the world. However, the tick species involved in the transmission of A. marginale in buffaloes in Brazil have not been identified. The objective of the present study was to determine the presence of A. marginale in ticks parasitizing water buffaloes. A total of 200 samples of Rhipicephalus microplus, Dermacentor nitens, Amblyomma cajennense, and Amblyomma maculatum were collected and tested by conventional and quantitative PCR for the presence of the msp1a and msp5 genes. In the present study, 35 ticks (17.5%) were positive for A. marginale DNA by qPCR analysis. The positive ticks belonged to four different species: R. microplus (22.2%), A. cajennense (13.8%), A. maculatum (16.0%), and D. nitens (10.0%). Individuals of the three developmental stages (larvae, nymphs, and adults) of R. microplus and A. cajennense were found to be positive for A. marginale, only nymphs and adults of A. maculatum were found to be positive, and finally, only adults of D. nitens were positive for A. marginale. Our results suggest that R. microplus, A. cajennense, A. maculatum, and D. nitens ticks may be involved in the transmission of A. marg-inale in buffaloes. However, while A. marg-inale PCR positive ticks were recorded, this does not indicate vector competence; only that the ticks may contain a blood meal from an infected host. Additionally, the results show that the strains of A. marginale from buffaloes and cattle are phylogenetically related. (C) 2015 Elsevier B.V. All rights reserved.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Dept Patol Vet, Sao Paulo, BrazilUniv Fed Rural Rio de Janeiro, Dept Parasitol, Rio De Janeiro, BrazilUniv Fed Para, Dept Clin Vet, Castanhal, Para, BrazilUniv Estadual Paulista, Dept Patol Vet, Sao Paulo, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Univ Fed Rural Rio de JaneiroUniv Fed ParaSilva, Jenevaldo Barbosa da [UNESP]Fonseca, Adivaldo Henrique daBarbosa, Jose Diomedes2018-11-26T15:28:05Z2018-11-26T15:28:05Z2015-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article38-41application/pdfhttp://dx.doi.org/10.1016/j.meegid.2015.07.027Infection Genetics And Evolution. Amsterdam: Elsevier Science Bv, v. 35, p. 38-41, 2015.1567-1348http://hdl.handle.net/11449/15855310.1016/j.meegid.2015.07.027WOS:000362607400006WOS000362607400006.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInfection Genetics And Evolution1,278info:eu-repo/semantics/openAccess2023-12-18T06:20:36Zoai:repositorio.unesp.br:11449/158553Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:41:52.672975Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular characterization of Anaplasma marginale in ticks naturally feeding on buffaloes |
title |
Molecular characterization of Anaplasma marginale in ticks naturally feeding on buffaloes |
spellingShingle |
Molecular characterization of Anaplasma marginale in ticks naturally feeding on buffaloes Silva, Jenevaldo Barbosa da [UNESP] Amblyomma cajennense Amblyomma maculatum Anaplasma marginale Dermacentor nitens Rhipicephalus microplus |
title_short |
Molecular characterization of Anaplasma marginale in ticks naturally feeding on buffaloes |
title_full |
Molecular characterization of Anaplasma marginale in ticks naturally feeding on buffaloes |
title_fullStr |
Molecular characterization of Anaplasma marginale in ticks naturally feeding on buffaloes |
title_full_unstemmed |
Molecular characterization of Anaplasma marginale in ticks naturally feeding on buffaloes |
title_sort |
Molecular characterization of Anaplasma marginale in ticks naturally feeding on buffaloes |
author |
Silva, Jenevaldo Barbosa da [UNESP] |
author_facet |
Silva, Jenevaldo Barbosa da [UNESP] Fonseca, Adivaldo Henrique da Barbosa, Jose Diomedes |
author_role |
author |
author2 |
Fonseca, Adivaldo Henrique da Barbosa, Jose Diomedes |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Fed Rural Rio de Janeiro Univ Fed Para |
dc.contributor.author.fl_str_mv |
Silva, Jenevaldo Barbosa da [UNESP] Fonseca, Adivaldo Henrique da Barbosa, Jose Diomedes |
dc.subject.por.fl_str_mv |
Amblyomma cajennense Amblyomma maculatum Anaplasma marginale Dermacentor nitens Rhipicephalus microplus |
topic |
Amblyomma cajennense Amblyomma maculatum Anaplasma marginale Dermacentor nitens Rhipicephalus microplus |
description |
Anaplasma marginale is the most prevalent pathogen transmitted by ticks in cattle in tropical and subtropical regions of the world. However, the tick species involved in the transmission of A. marginale in buffaloes in Brazil have not been identified. The objective of the present study was to determine the presence of A. marginale in ticks parasitizing water buffaloes. A total of 200 samples of Rhipicephalus microplus, Dermacentor nitens, Amblyomma cajennense, and Amblyomma maculatum were collected and tested by conventional and quantitative PCR for the presence of the msp1a and msp5 genes. In the present study, 35 ticks (17.5%) were positive for A. marginale DNA by qPCR analysis. The positive ticks belonged to four different species: R. microplus (22.2%), A. cajennense (13.8%), A. maculatum (16.0%), and D. nitens (10.0%). Individuals of the three developmental stages (larvae, nymphs, and adults) of R. microplus and A. cajennense were found to be positive for A. marginale, only nymphs and adults of A. maculatum were found to be positive, and finally, only adults of D. nitens were positive for A. marginale. Our results suggest that R. microplus, A. cajennense, A. maculatum, and D. nitens ticks may be involved in the transmission of A. marg-inale in buffaloes. However, while A. marg-inale PCR positive ticks were recorded, this does not indicate vector competence; only that the ticks may contain a blood meal from an infected host. Additionally, the results show that the strains of A. marginale from buffaloes and cattle are phylogenetically related. (C) 2015 Elsevier B.V. All rights reserved. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-01 2018-11-26T15:28:05Z 2018-11-26T15:28:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.meegid.2015.07.027 Infection Genetics And Evolution. Amsterdam: Elsevier Science Bv, v. 35, p. 38-41, 2015. 1567-1348 http://hdl.handle.net/11449/158553 10.1016/j.meegid.2015.07.027 WOS:000362607400006 WOS000362607400006.pdf |
url |
http://dx.doi.org/10.1016/j.meegid.2015.07.027 http://hdl.handle.net/11449/158553 |
identifier_str_mv |
Infection Genetics And Evolution. Amsterdam: Elsevier Science Bv, v. 35, p. 38-41, 2015. 1567-1348 10.1016/j.meegid.2015.07.027 WOS:000362607400006 WOS000362607400006.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Infection Genetics And Evolution 1,278 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
38-41 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129235501449216 |