Potential application for antimicrobial and antileukemic therapy of a flavonoid-rich fraction of Camellia sinensis

• Autor(a) principal: de Souza, Mariana C.
• Data de Publicação: 2022
• Outros Autores: de Souza Mesquita, Leonardo M., Pena, Fabíola L., Tamborlin, Leticia [UNESP], da Silva, Laise C., Viganó, Juliane, Antunes, Adriane E. Costa, Luchessi, Augusto D. [UNESP], Duarte, Marta C. Teixeira, Barbero, Gerardo F., Rostagno, Mauricio A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.focha.2022.100042; http://hdl.handle.net/11449/246928
• topic: Cancer therapy; Coupled extraction techniques; Flavonoids; Theaflavin; THP-1

The antimicrobial and antileukemic effect of a purified fraction of flavonoids from the leaves of Camellia sinensis was evaluated. An extraction yield of 9.77 mg.g-1 total flavonoids was recovered through a pressurized liquid extraction associated with solid-phase extraction. This fraction was tested against pathogenic microorganisms (Staphylococcus, Salmonella, and Enterococcus), considering the minimum inhibitory concentration. In addition, the human monocyte cell line THP-1, derived from a patient with acute monocytic leukemia, was used for the antitumor assay. The results show that the flavonoid-rich fraction obtained by coupling a Pressurized Liquids Extraction in-line with a Solid Phase Extraction (PLE-SPE) has a high antimicrobial effect and resulted in cell cycle blockage G0 / G1, increased DNA fragmentation, and altered leukemic cell morphology. These results suggest that a flavonoid-rich fraction obtained from Camellia sinensis can be applied as potential adjuvants in chemotherapy treatment to mitigate the side effects caused by chemotherapy or even as a supplement to cancer therapy.