Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12867-015-0037-5 http://hdl.handle.net/11449/231350 |
Resumo: | Background: Box C/D snoRNPs are responsible for rRNA methylation and processing, and are formed by snoRNAs and four conserved proteins, Nop1, Nop56, Nop58 and Snu13. The snoRNP assembly is a stepwise process, involving other protein complexes, among which the R2TP and Hsp90 chaperone. Nop17, also known as Pih1, has been shown to be a constituent of the R2TP (Rvb1, Rvb2, Tah1, Pih1) and to participate in box C/D snoRNP assembly by its interaction with Nop58. The molecular function of Nop17, however, has not yet been described. Results: To shed light on the role played by Nop17 in the maturation of snoRNP, here we analyzed the interactions domains of Nop58 - Nop17 - Tah1 and the importance of ATP to the interaction between Nop17 and the ATPase Rvb1/2. Conclusions: Based on the results shown here, we propose a model for the assembly of box C/D snoRNP, according to which R2TP complex is important for reducing the affinity of Nop58 for snoRNA, and for the binding of the other snoRNP subunits. |
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Repositório Institucional da UNESP |
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spelling |
Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complexbox C/D snoRNP assemblyNop17R2TP complexBackground: Box C/D snoRNPs are responsible for rRNA methylation and processing, and are formed by snoRNAs and four conserved proteins, Nop1, Nop56, Nop58 and Snu13. The snoRNP assembly is a stepwise process, involving other protein complexes, among which the R2TP and Hsp90 chaperone. Nop17, also known as Pih1, has been shown to be a constituent of the R2TP (Rvb1, Rvb2, Tah1, Pih1) and to participate in box C/D snoRNP assembly by its interaction with Nop58. The molecular function of Nop17, however, has not yet been described. Results: To shed light on the role played by Nop17 in the maturation of snoRNP, here we analyzed the interactions domains of Nop58 - Nop17 - Tah1 and the importance of ATP to the interaction between Nop17 and the ATPase Rvb1/2. Conclusions: Based on the results shown here, we propose a model for the assembly of box C/D snoRNP, according to which R2TP complex is important for reducing the affinity of Nop58 for snoRNA, and for the binding of the other snoRNP subunits.Institute of Chemistry, University of São Paulo, Department of Biochemistry, Av. Prof. Lineu Prestes 748Institute of Biological Sciences, Federal University of Goiás, Department of Biochemistry and Molecular BiologySão Paulo State University, Department of Biological Sciences, School of PharmacySão Paulo State University, Department of Biological Sciences, School of PharmacyUniversidade de São Paulo (USP)Universidade Federal de Goiás (UFG)Universidade Estadual Paulista (UNESP)Prieto, Marcela B.Georg, Raphaela C.Gonzales-Zubiate, Fernando A.Luz, Juliana S. [UNESP]Oliveira, Carla C.2022-04-29T08:44:54Z2022-04-29T08:44:54Z2015-03-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s12867-015-0037-5BMC Molecular Biology, v. 16, n. 1, 2015.1471-2199http://hdl.handle.net/11449/23135010.1186/s12867-015-0037-52-s2.0-84925585165Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Molecular Biologyinfo:eu-repo/semantics/openAccess2022-04-29T08:44:54Zoai:repositorio.unesp.br:11449/231350Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:43:25.419737Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex |
title |
Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex |
spellingShingle |
Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex Prieto, Marcela B. box C/D snoRNP assembly Nop17 R2TP complex |
title_short |
Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex |
title_full |
Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex |
title_fullStr |
Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex |
title_full_unstemmed |
Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex |
title_sort |
Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex |
author |
Prieto, Marcela B. |
author_facet |
Prieto, Marcela B. Georg, Raphaela C. Gonzales-Zubiate, Fernando A. Luz, Juliana S. [UNESP] Oliveira, Carla C. |
author_role |
author |
author2 |
Georg, Raphaela C. Gonzales-Zubiate, Fernando A. Luz, Juliana S. [UNESP] Oliveira, Carla C. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal de Goiás (UFG) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Prieto, Marcela B. Georg, Raphaela C. Gonzales-Zubiate, Fernando A. Luz, Juliana S. [UNESP] Oliveira, Carla C. |
dc.subject.por.fl_str_mv |
box C/D snoRNP assembly Nop17 R2TP complex |
topic |
box C/D snoRNP assembly Nop17 R2TP complex |
description |
Background: Box C/D snoRNPs are responsible for rRNA methylation and processing, and are formed by snoRNAs and four conserved proteins, Nop1, Nop56, Nop58 and Snu13. The snoRNP assembly is a stepwise process, involving other protein complexes, among which the R2TP and Hsp90 chaperone. Nop17, also known as Pih1, has been shown to be a constituent of the R2TP (Rvb1, Rvb2, Tah1, Pih1) and to participate in box C/D snoRNP assembly by its interaction with Nop58. The molecular function of Nop17, however, has not yet been described. Results: To shed light on the role played by Nop17 in the maturation of snoRNP, here we analyzed the interactions domains of Nop58 - Nop17 - Tah1 and the importance of ATP to the interaction between Nop17 and the ATPase Rvb1/2. Conclusions: Based on the results shown here, we propose a model for the assembly of box C/D snoRNP, according to which R2TP complex is important for reducing the affinity of Nop58 for snoRNA, and for the binding of the other snoRNP subunits. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-18 2022-04-29T08:44:54Z 2022-04-29T08:44:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12867-015-0037-5 BMC Molecular Biology, v. 16, n. 1, 2015. 1471-2199 http://hdl.handle.net/11449/231350 10.1186/s12867-015-0037-5 2-s2.0-84925585165 |
url |
http://dx.doi.org/10.1186/s12867-015-0037-5 http://hdl.handle.net/11449/231350 |
identifier_str_mv |
BMC Molecular Biology, v. 16, n. 1, 2015. 1471-2199 10.1186/s12867-015-0037-5 2-s2.0-84925585165 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Molecular Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129546423107584 |