Microcystins and cylindrospermopsins molecular markers for the detection of toxic cyanobacteria: A case study of northeastern Brazilian reservoirs
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/220661 |
Resumo: | The presence of potentially toxic cyanobacteria in 10 northeastern Brazilian reservoirs used for public water supply was evaluated with molecular markers specific to genes of microcystins (MC) and cylindrospermopsins (CYN). The detection of the MC genes mcyA and mcyB was determined using 3 different primer pairs. Genes of CYN were assessed by PCR amplification of PKS and PS genes. Amplicons of the different cyanotoxin genes were subjected to sequencing. Genes coding for MC (mcyA and/or mcyB) were found in all of the analysed samples. Despite the presence of the mcyB gene and the subsequent immunological detection of MC, the absence of mcyA was observed in several samples. In general, the primers targeting the PKS gene were more specific, while those for the PS gene gave uneven PCR amplification. In addition, the detection of CYN was only possible when the samples were positive for both the PKS and the PS genes. These findings show that, when working with environmental samples, the use of molecular markers for the detection of potentially toxic cyanobacteria may generate variable results, and consideration should be given to possible phytogeographic genetic variations. |
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Microcystins and cylindrospermopsins molecular markers for the detection of toxic cyanobacteria: A case study of northeastern Brazilian reservoirsEarly warningGenotype compositionPeptide synthetasePublic water supplyWater monitoringThe presence of potentially toxic cyanobacteria in 10 northeastern Brazilian reservoirs used for public water supply was evaluated with molecular markers specific to genes of microcystins (MC) and cylindrospermopsins (CYN). The detection of the MC genes mcyA and mcyB was determined using 3 different primer pairs. Genes of CYN were assessed by PCR amplification of PKS and PS genes. Amplicons of the different cyanotoxin genes were subjected to sequencing. Genes coding for MC (mcyA and/or mcyB) were found in all of the analysed samples. Despite the presence of the mcyB gene and the subsequent immunological detection of MC, the absence of mcyA was observed in several samples. In general, the primers targeting the PKS gene were more specific, while those for the PS gene gave uneven PCR amplification. In addition, the detection of CYN was only possible when the samples were positive for both the PKS and the PS genes. These findings show that, when working with environmental samples, the use of molecular markers for the detection of potentially toxic cyanobacteria may generate variable results, and consideration should be given to possible phytogeographic genetic variations.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Ciências Biológicas Escola Superior de Agricultura Luiz de Queiroz Universidade de São PauloPrograma de Pós-Graduação em Ciências Biológicas Universidade Estadual Paulista Júlio de Mesquita FilhoPrograma de Pós-Graduação em Ciências Biológicas Universidade Estadual Paulista Júlio de Mesquita FilhoFAPESP: 2008/56534-5FAPESP: 2013/11306-3FAPESP: 2014/01913-2Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Lorenzi, Adriana SturionChia, Mathias AhiiPiccin-Santos, Viviane [UNESP]Bittencourt-Oliveira, Maria Do Carmo2022-04-28T19:04:22Z2022-04-28T19:04:22Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article269-282Limnetica, v. 34, n. 2, p. 269-282, 2015.1989-18060213-8409http://hdl.handle.net/11449/2206612-s2.0-84978639744Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLimneticainfo:eu-repo/semantics/openAccess2022-04-28T19:04:22Zoai:repositorio.unesp.br:11449/220661Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:24:42.071476Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Microcystins and cylindrospermopsins molecular markers for the detection of toxic cyanobacteria: A case study of northeastern Brazilian reservoirs |
title |
Microcystins and cylindrospermopsins molecular markers for the detection of toxic cyanobacteria: A case study of northeastern Brazilian reservoirs |
spellingShingle |
Microcystins and cylindrospermopsins molecular markers for the detection of toxic cyanobacteria: A case study of northeastern Brazilian reservoirs Lorenzi, Adriana Sturion Early warning Genotype composition Peptide synthetase Public water supply Water monitoring |
title_short |
Microcystins and cylindrospermopsins molecular markers for the detection of toxic cyanobacteria: A case study of northeastern Brazilian reservoirs |
title_full |
Microcystins and cylindrospermopsins molecular markers for the detection of toxic cyanobacteria: A case study of northeastern Brazilian reservoirs |
title_fullStr |
Microcystins and cylindrospermopsins molecular markers for the detection of toxic cyanobacteria: A case study of northeastern Brazilian reservoirs |
title_full_unstemmed |
Microcystins and cylindrospermopsins molecular markers for the detection of toxic cyanobacteria: A case study of northeastern Brazilian reservoirs |
title_sort |
Microcystins and cylindrospermopsins molecular markers for the detection of toxic cyanobacteria: A case study of northeastern Brazilian reservoirs |
author |
Lorenzi, Adriana Sturion |
author_facet |
Lorenzi, Adriana Sturion Chia, Mathias Ahii Piccin-Santos, Viviane [UNESP] Bittencourt-Oliveira, Maria Do Carmo |
author_role |
author |
author2 |
Chia, Mathias Ahii Piccin-Santos, Viviane [UNESP] Bittencourt-Oliveira, Maria Do Carmo |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Lorenzi, Adriana Sturion Chia, Mathias Ahii Piccin-Santos, Viviane [UNESP] Bittencourt-Oliveira, Maria Do Carmo |
dc.subject.por.fl_str_mv |
Early warning Genotype composition Peptide synthetase Public water supply Water monitoring |
topic |
Early warning Genotype composition Peptide synthetase Public water supply Water monitoring |
description |
The presence of potentially toxic cyanobacteria in 10 northeastern Brazilian reservoirs used for public water supply was evaluated with molecular markers specific to genes of microcystins (MC) and cylindrospermopsins (CYN). The detection of the MC genes mcyA and mcyB was determined using 3 different primer pairs. Genes of CYN were assessed by PCR amplification of PKS and PS genes. Amplicons of the different cyanotoxin genes were subjected to sequencing. Genes coding for MC (mcyA and/or mcyB) were found in all of the analysed samples. Despite the presence of the mcyB gene and the subsequent immunological detection of MC, the absence of mcyA was observed in several samples. In general, the primers targeting the PKS gene were more specific, while those for the PS gene gave uneven PCR amplification. In addition, the detection of CYN was only possible when the samples were positive for both the PKS and the PS genes. These findings show that, when working with environmental samples, the use of molecular markers for the detection of potentially toxic cyanobacteria may generate variable results, and consideration should be given to possible phytogeographic genetic variations. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2022-04-28T19:04:22Z 2022-04-28T19:04:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Limnetica, v. 34, n. 2, p. 269-282, 2015. 1989-1806 0213-8409 http://hdl.handle.net/11449/220661 2-s2.0-84978639744 |
identifier_str_mv |
Limnetica, v. 34, n. 2, p. 269-282, 2015. 1989-1806 0213-8409 2-s2.0-84978639744 |
url |
http://hdl.handle.net/11449/220661 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Limnetica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
269-282 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129517865140224 |