Mast cells in the pathophysiology of Duchenne muscular dystrophy in Golden Retriever dogs

Detalhes bibliográficos
Autor(a) principal: Martins, Isabela M. [UNESP]
Data de Publicação: 2020
Outros Autores: Malvestio, Lygia M.M. [UNESP], Engracia-Filho, Jair R., Claudiano, Gustavo S. [UNESP], Moraes, Flávio R. [UNESP], Moraes, Julieta R.E. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1678-5150-pvb-6340
http://hdl.handle.net/11449/207064
Resumo: The Golden Retriever muscular dystrophy (GRMD) is one of the best models of Duchenne muscular dystrophy (DMD), with similar genotypic and phenotypic manifestations. Progressive proliferation of connective tissue in the endomysium of the muscle fibers occurs in parallel with the clinical course of the disease in GRMD animals. Previous studies suggest a relationship between mast cells and the deposition of fibrous tissue due to the release of mediators that recruit fibroblasts. The aim of this study was to evaluate the presence of mast cells and their relationship with muscle injury and fibrosis in GRMD dogs of different ages. Samples of muscle groups from six GRMD and four control dogs, aged 2 to 8 months, were collected and analyzed. The samples were processed and stained with HE, toluidine blue, and Azan trichrome. Our results showed that there was a significant increase in infiltration of mast cells in all muscle groups of GRMD dogs compared to the control group. The average number of mast cells, as well as the deposition of fibrous tissue, decreased with age in GRMD dogs. In the control group, all muscle types showed a significant increase in the amount of collagenous tissue. This suggests increased mast cell degranulation occurred in younger GRMD dogs, resulting in increased interstitial space and fibrous tissue in muscle, which then gradually decreased over time as the dogs aged. However, further studies are needed to clarify the role of mast cells in the pathogenesis of fibrosis.
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spelling Mast cells in the pathophysiology of Duchenne muscular dystrophy in Golden Retriever dogsCanineDogsDuchenne muscular dystrophyFibrosisGolden Retriever dogsMast cellsMuscular dystrophyPathogenesisThe Golden Retriever muscular dystrophy (GRMD) is one of the best models of Duchenne muscular dystrophy (DMD), with similar genotypic and phenotypic manifestations. Progressive proliferation of connective tissue in the endomysium of the muscle fibers occurs in parallel with the clinical course of the disease in GRMD animals. Previous studies suggest a relationship between mast cells and the deposition of fibrous tissue due to the release of mediators that recruit fibroblasts. The aim of this study was to evaluate the presence of mast cells and their relationship with muscle injury and fibrosis in GRMD dogs of different ages. Samples of muscle groups from six GRMD and four control dogs, aged 2 to 8 months, were collected and analyzed. The samples were processed and stained with HE, toluidine blue, and Azan trichrome. Our results showed that there was a significant increase in infiltration of mast cells in all muscle groups of GRMD dogs compared to the control group. The average number of mast cells, as well as the deposition of fibrous tissue, decreased with age in GRMD dogs. In the control group, all muscle types showed a significant increase in the amount of collagenous tissue. This suggests increased mast cell degranulation occurred in younger GRMD dogs, resulting in increased interstitial space and fibrous tissue in muscle, which then gradually decreased over time as the dogs aged. However, further studies are needed to clarify the role of mast cells in the pathogenesis of fibrosis.Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista “Júlio de Mesquita Filho” (Unesp), Via Prof. Paulo Donato Castellane Km 5Escola de Medicina Veterinária IMED, Rua Gen. Prestes Guimarães 304, Vila RodriguesGraduate Program in Animal Science Escola de Ciências da Vida Pontifícia Universidade Católica (PUC), Rua Imaculada Conceição 1155,Instituto de Biodiversidade e Florestas Universidade Federal do Oeste do Pará (UFOPA) Campus Santarém, Av. Vera Paz s/nDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista “Júlio de Mesquita Filho” (Unesp), Via Prof. Paulo Donato Castellane Km 5Universidade Estadual Paulista (Unesp)IMEDPontifícia Universidade Católica (PUC)Universidade Federal do Oeste do Pará (UFOPA)Martins, Isabela M. [UNESP]Malvestio, Lygia M.M. [UNESP]Engracia-Filho, Jair R.Claudiano, Gustavo S. [UNESP]Moraes, Flávio R. [UNESP]Moraes, Julieta R.E. [UNESP]2021-06-25T10:48:21Z2021-06-25T10:48:21Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article791-797application/pdfhttp://dx.doi.org/10.1590/1678-5150-pvb-6340Pesquisa Veterinaria Brasileira, v. 40, n. 10, p. 791-797, 2020.1678-51500100-736Xhttp://hdl.handle.net/11449/20706410.1590/1678-5150-pvb-6340S0100-736X20200010007912-s2.0-85098655238S0100-736X2020001000791.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2024-06-07T13:02:05Zoai:repositorio.unesp.br:11449/207064Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:57:03.736690Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Mast cells in the pathophysiology of Duchenne muscular dystrophy in Golden Retriever dogs
title Mast cells in the pathophysiology of Duchenne muscular dystrophy in Golden Retriever dogs
spellingShingle Mast cells in the pathophysiology of Duchenne muscular dystrophy in Golden Retriever dogs
Martins, Isabela M. [UNESP]
Canine
Dogs
Duchenne muscular dystrophy
Fibrosis
Golden Retriever dogs
Mast cells
Muscular dystrophy
Pathogenesis
title_short Mast cells in the pathophysiology of Duchenne muscular dystrophy in Golden Retriever dogs
title_full Mast cells in the pathophysiology of Duchenne muscular dystrophy in Golden Retriever dogs
title_fullStr Mast cells in the pathophysiology of Duchenne muscular dystrophy in Golden Retriever dogs
title_full_unstemmed Mast cells in the pathophysiology of Duchenne muscular dystrophy in Golden Retriever dogs
title_sort Mast cells in the pathophysiology of Duchenne muscular dystrophy in Golden Retriever dogs
author Martins, Isabela M. [UNESP]
author_facet Martins, Isabela M. [UNESP]
Malvestio, Lygia M.M. [UNESP]
Engracia-Filho, Jair R.
Claudiano, Gustavo S. [UNESP]
Moraes, Flávio R. [UNESP]
Moraes, Julieta R.E. [UNESP]
author_role author
author2 Malvestio, Lygia M.M. [UNESP]
Engracia-Filho, Jair R.
Claudiano, Gustavo S. [UNESP]
Moraes, Flávio R. [UNESP]
Moraes, Julieta R.E. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
IMED
Pontifícia Universidade Católica (PUC)
Universidade Federal do Oeste do Pará (UFOPA)
dc.contributor.author.fl_str_mv Martins, Isabela M. [UNESP]
Malvestio, Lygia M.M. [UNESP]
Engracia-Filho, Jair R.
Claudiano, Gustavo S. [UNESP]
Moraes, Flávio R. [UNESP]
Moraes, Julieta R.E. [UNESP]
dc.subject.por.fl_str_mv Canine
Dogs
Duchenne muscular dystrophy
Fibrosis
Golden Retriever dogs
Mast cells
Muscular dystrophy
Pathogenesis
topic Canine
Dogs
Duchenne muscular dystrophy
Fibrosis
Golden Retriever dogs
Mast cells
Muscular dystrophy
Pathogenesis
description The Golden Retriever muscular dystrophy (GRMD) is one of the best models of Duchenne muscular dystrophy (DMD), with similar genotypic and phenotypic manifestations. Progressive proliferation of connective tissue in the endomysium of the muscle fibers occurs in parallel with the clinical course of the disease in GRMD animals. Previous studies suggest a relationship between mast cells and the deposition of fibrous tissue due to the release of mediators that recruit fibroblasts. The aim of this study was to evaluate the presence of mast cells and their relationship with muscle injury and fibrosis in GRMD dogs of different ages. Samples of muscle groups from six GRMD and four control dogs, aged 2 to 8 months, were collected and analyzed. The samples were processed and stained with HE, toluidine blue, and Azan trichrome. Our results showed that there was a significant increase in infiltration of mast cells in all muscle groups of GRMD dogs compared to the control group. The average number of mast cells, as well as the deposition of fibrous tissue, decreased with age in GRMD dogs. In the control group, all muscle types showed a significant increase in the amount of collagenous tissue. This suggests increased mast cell degranulation occurred in younger GRMD dogs, resulting in increased interstitial space and fibrous tissue in muscle, which then gradually decreased over time as the dogs aged. However, further studies are needed to clarify the role of mast cells in the pathogenesis of fibrosis.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-01
2021-06-25T10:48:21Z
2021-06-25T10:48:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1678-5150-pvb-6340
Pesquisa Veterinaria Brasileira, v. 40, n. 10, p. 791-797, 2020.
1678-5150
0100-736X
http://hdl.handle.net/11449/207064
10.1590/1678-5150-pvb-6340
S0100-736X2020001000791
2-s2.0-85098655238
S0100-736X2020001000791.pdf
url http://dx.doi.org/10.1590/1678-5150-pvb-6340
http://hdl.handle.net/11449/207064
identifier_str_mv Pesquisa Veterinaria Brasileira, v. 40, n. 10, p. 791-797, 2020.
1678-5150
0100-736X
10.1590/1678-5150-pvb-6340
S0100-736X2020001000791
2-s2.0-85098655238
S0100-736X2020001000791.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pesquisa Veterinaria Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 791-797
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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