DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4025/actascibiolsci.v39i1.33024 http://hdl.handle.net/11449/232598 |
Resumo: | Growers appreciate Cattleya walkeriana and C. loddigesii due to striking shape and rarity. Thus, this study aimed to evaluate the feasibility of DNA barcode regions, namely ITS1, ITS2 and rpoC1, to discriminate between C. walkeriana and C. loddigesii species. DNA barcode regions were successfully amplified using primers designed to amplify plants. We also included sequences from public databases in order to test if these regions were able to discriminate C. walkeriana and C. loddigesii from other Cattleya species. These regions, and their combinations, demonstrated that the ITS1+ITS2 had the highest average interspecific distance (11.1%), followed by rpoC1 (1.06%). For species discrimination, ITS1+ITS2 provided the best results. The combined data set of ITS1+ITS2+rpoC1 also discriminated both species, but did not result in higher rates of discrimination. These results indicate that ITS region is the best option for molecular identification of these two species and from some other species of this genus. |
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DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesiiRegiões de DNA barcode para diferenciar Cattleya walkeriana e C. loddigesiiGenetic variationOrchid improvementSpecies separationGrowers appreciate Cattleya walkeriana and C. loddigesii due to striking shape and rarity. Thus, this study aimed to evaluate the feasibility of DNA barcode regions, namely ITS1, ITS2 and rpoC1, to discriminate between C. walkeriana and C. loddigesii species. DNA barcode regions were successfully amplified using primers designed to amplify plants. We also included sequences from public databases in order to test if these regions were able to discriminate C. walkeriana and C. loddigesii from other Cattleya species. These regions, and their combinations, demonstrated that the ITS1+ITS2 had the highest average interspecific distance (11.1%), followed by rpoC1 (1.06%). For species discrimination, ITS1+ITS2 provided the best results. The combined data set of ITS1+ITS2+rpoC1 also discriminated both species, but did not result in higher rates of discrimination. These results indicate that ITS region is the best option for molecular identification of these two species and from some other species of this genus.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade de São PauloDepartamento de Genética Instituto de Biociências Universidade Estadual PaulistaInstituto de Biotecnologia Universidade Estadual PaulistaUniversidade Estadual do Centro-Oeste, PR-153, Km 7Faculdade de Ciências Agronômicas Universidade Estadual Paulista, Rua José Barbosa de Barros, 1780Escola Superior de Agricultura “Luiz de Queiroz” Universidade de São PauloDepartamento de Genética Instituto de Biociências Universidade Estadual PaulistaInstituto de Biotecnologia Universidade Estadual PaulistaFaculdade de Ciências Agronômicas Universidade Estadual Paulista, Rua José Barbosa de Barros, 1780Universidade Estadual Paulista (UNESP)Universidade Estadual do Centro-OesteUniversidade de São Paulo (USP)Rivera-Jiménez, Hernando [UNESP]Rossini, Bruno César [UNESP]Tambarussi, Evandro Vagner [UNESP]Veasey, Elizabeth AnnIbanes, BrunaMarino, Celso Luis [UNESP]2022-04-29T23:53:04Z2022-04-29T23:53:04Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article45-52http://dx.doi.org/10.4025/actascibiolsci.v39i1.33024Acta Scientiarum - Biological Sciences, v. 39, n. 1, p. 45-52, 2017.1807-863X1679-9283http://hdl.handle.net/11449/23259810.4025/actascibiolsci.v39i1.330242-s2.0-85019400893Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Scientiarum - Biological Sciencesinfo:eu-repo/semantics/openAccess2022-04-29T23:53:04Zoai:repositorio.unesp.br:11449/232598Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:36:42.562814Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii Regiões de DNA barcode para diferenciar Cattleya walkeriana e C. loddigesii |
title |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii |
spellingShingle |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii Rivera-Jiménez, Hernando [UNESP] Genetic variation Orchid improvement Species separation |
title_short |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii |
title_full |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii |
title_fullStr |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii |
title_full_unstemmed |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii |
title_sort |
DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii |
author |
Rivera-Jiménez, Hernando [UNESP] |
author_facet |
Rivera-Jiménez, Hernando [UNESP] Rossini, Bruno César [UNESP] Tambarussi, Evandro Vagner [UNESP] Veasey, Elizabeth Ann Ibanes, Bruna Marino, Celso Luis [UNESP] |
author_role |
author |
author2 |
Rossini, Bruno César [UNESP] Tambarussi, Evandro Vagner [UNESP] Veasey, Elizabeth Ann Ibanes, Bruna Marino, Celso Luis [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual do Centro-Oeste Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Rivera-Jiménez, Hernando [UNESP] Rossini, Bruno César [UNESP] Tambarussi, Evandro Vagner [UNESP] Veasey, Elizabeth Ann Ibanes, Bruna Marino, Celso Luis [UNESP] |
dc.subject.por.fl_str_mv |
Genetic variation Orchid improvement Species separation |
topic |
Genetic variation Orchid improvement Species separation |
description |
Growers appreciate Cattleya walkeriana and C. loddigesii due to striking shape and rarity. Thus, this study aimed to evaluate the feasibility of DNA barcode regions, namely ITS1, ITS2 and rpoC1, to discriminate between C. walkeriana and C. loddigesii species. DNA barcode regions were successfully amplified using primers designed to amplify plants. We also included sequences from public databases in order to test if these regions were able to discriminate C. walkeriana and C. loddigesii from other Cattleya species. These regions, and their combinations, demonstrated that the ITS1+ITS2 had the highest average interspecific distance (11.1%), followed by rpoC1 (1.06%). For species discrimination, ITS1+ITS2 provided the best results. The combined data set of ITS1+ITS2+rpoC1 also discriminated both species, but did not result in higher rates of discrimination. These results indicate that ITS region is the best option for molecular identification of these two species and from some other species of this genus. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2022-04-29T23:53:04Z 2022-04-29T23:53:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4025/actascibiolsci.v39i1.33024 Acta Scientiarum - Biological Sciences, v. 39, n. 1, p. 45-52, 2017. 1807-863X 1679-9283 http://hdl.handle.net/11449/232598 10.4025/actascibiolsci.v39i1.33024 2-s2.0-85019400893 |
url |
http://dx.doi.org/10.4025/actascibiolsci.v39i1.33024 http://hdl.handle.net/11449/232598 |
identifier_str_mv |
Acta Scientiarum - Biological Sciences, v. 39, n. 1, p. 45-52, 2017. 1807-863X 1679-9283 10.4025/actascibiolsci.v39i1.33024 2-s2.0-85019400893 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Scientiarum - Biological Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
45-52 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128834258599936 |