Experimental model of intestinal endometriosis: pilot study

Detalhes bibliográficos
Autor(a) principal: Rossini, Lucio Giovanni Battista
Data de Publicação: 2015
Outros Autores: Hossne, Rogério Saad [UNESP], Zago, Rodrigo de Rezende, Silva, Anna Carolina, Kalenski, Tabatha, Averbach, Marcelo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.9734/bjast/2015/13615
http://hdl.handle.net/11449/136812
Resumo: Aims: The objective of this study is to create an experimental model of intestinal endometriosis in pigs, which might allow better understanding of deep infiltrating endometriosis and development of new treatment techniques. As secondary objective, we intend to create endometrial implants accessible by transrectal ultrasonography (TRUS). Study Design: Surgical experimental study in swine. Place and Duration of Study: This study was performed at the Instituto de Ensino e Pesquisa do Hospital Sírio-Libanês, São Paulo, Brazil, between January 2012 and December 2012. Methodology: Two sexually mature female minipigBR pigs underwent two laparotomies (each animal). The first laparotomy was performed to implant two fragments of autologous endometrium in the rectal wall. The second one was performed thirty days later to visualize, measure and obtain tissue of the site of the implants for histopathology study. A TRUS study was performed prior to the second surgery. The Institution’s Animal Utilization Study Committee approved the study. Results: In the first laparotomy a 5-cm segment of right uterine horn was resected. The endometrium was separated from the myometrium through sub-endometrial saline injection. Two endometrial fragments (1.0 x 2.0 cm) were dissected and sutured in the intra peritoneal anterior rectal wall of the animals. Thirty days later, all implants were identified during preoperative TRUS. “En-bloc” resection of the intestinal segment with the implants was performed during the second surgery. The autologous implants of endometrium invaded the muscular layer in one of the two animals. Conclusion: We demonstrated that the creation of an animal model of deep infiltrating endometriosis with intestinal involvement is feasible through a simple surgical technique. We believe that this model can be applied in experimental and clinical studies but further studies are necessary to refine the technique.
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spelling Experimental model of intestinal endometriosis: pilot studyEndometriosisDeep infiltrating endometriosisExperimental animal modelSwineAims: The objective of this study is to create an experimental model of intestinal endometriosis in pigs, which might allow better understanding of deep infiltrating endometriosis and development of new treatment techniques. As secondary objective, we intend to create endometrial implants accessible by transrectal ultrasonography (TRUS). Study Design: Surgical experimental study in swine. Place and Duration of Study: This study was performed at the Instituto de Ensino e Pesquisa do Hospital Sírio-Libanês, São Paulo, Brazil, between January 2012 and December 2012. Methodology: Two sexually mature female minipigBR pigs underwent two laparotomies (each animal). The first laparotomy was performed to implant two fragments of autologous endometrium in the rectal wall. The second one was performed thirty days later to visualize, measure and obtain tissue of the site of the implants for histopathology study. A TRUS study was performed prior to the second surgery. The Institution’s Animal Utilization Study Committee approved the study. Results: In the first laparotomy a 5-cm segment of right uterine horn was resected. The endometrium was separated from the myometrium through sub-endometrial saline injection. Two endometrial fragments (1.0 x 2.0 cm) were dissected and sutured in the intra peritoneal anterior rectal wall of the animals. Thirty days later, all implants were identified during preoperative TRUS. “En-bloc” resection of the intestinal segment with the implants was performed during the second surgery. The autologous implants of endometrium invaded the muscular layer in one of the two animals. Conclusion: We demonstrated that the creation of an animal model of deep infiltrating endometriosis with intestinal involvement is feasible through a simple surgical technique. We believe that this model can be applied in experimental and clinical studies but further studies are necessary to refine the technique.Hospital Sírio-Libanês, São Paulo, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina de Botucatu (FMB), Departamento de Cirurgia e Ortopedia, Botucatu, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina de Botucatu (FMB), Departamento de Cirurgia e Ortopedia, Botucatu, SP, BrasilHospital Sírio-LibanêsUniversidade Estadual Paulista (Unesp)Rossini, Lucio Giovanni BattistaHossne, Rogério Saad [UNESP]Zago, Rodrigo de RezendeSilva, Anna CarolinaKalenski, TabathaAverbach, Marcelo2016-04-01T18:42:41Z2016-04-01T18:42:41Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article449-455application/pdfhttp://dx.doi.org/10.9734/bjast/2015/13615British Journal of Applied Science and Technology, v. 7, n. 5, p. 449-455, 2015.2231-0843http://hdl.handle.net/11449/13681210.9734/bjast/2015/13615ISSN2231-0843-2015-07-05-449-455.pdf952634515659047795263451565904779526345156590477Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBritish Journal of Applied Science and Technologyinfo:eu-repo/semantics/openAccess2024-01-09T06:30:13Zoai:repositorio.unesp.br:11449/136812Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-09T06:30:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Experimental model of intestinal endometriosis: pilot study
title Experimental model of intestinal endometriosis: pilot study
spellingShingle Experimental model of intestinal endometriosis: pilot study
Rossini, Lucio Giovanni Battista
Endometriosis
Deep infiltrating endometriosis
Experimental animal model
Swine
title_short Experimental model of intestinal endometriosis: pilot study
title_full Experimental model of intestinal endometriosis: pilot study
title_fullStr Experimental model of intestinal endometriosis: pilot study
title_full_unstemmed Experimental model of intestinal endometriosis: pilot study
title_sort Experimental model of intestinal endometriosis: pilot study
author Rossini, Lucio Giovanni Battista
author_facet Rossini, Lucio Giovanni Battista
Hossne, Rogério Saad [UNESP]
Zago, Rodrigo de Rezende
Silva, Anna Carolina
Kalenski, Tabatha
Averbach, Marcelo
author_role author
author2 Hossne, Rogério Saad [UNESP]
Zago, Rodrigo de Rezende
Silva, Anna Carolina
Kalenski, Tabatha
Averbach, Marcelo
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Hospital Sírio-Libanês
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Rossini, Lucio Giovanni Battista
Hossne, Rogério Saad [UNESP]
Zago, Rodrigo de Rezende
Silva, Anna Carolina
Kalenski, Tabatha
Averbach, Marcelo
dc.subject.por.fl_str_mv Endometriosis
Deep infiltrating endometriosis
Experimental animal model
Swine
topic Endometriosis
Deep infiltrating endometriosis
Experimental animal model
Swine
description Aims: The objective of this study is to create an experimental model of intestinal endometriosis in pigs, which might allow better understanding of deep infiltrating endometriosis and development of new treatment techniques. As secondary objective, we intend to create endometrial implants accessible by transrectal ultrasonography (TRUS). Study Design: Surgical experimental study in swine. Place and Duration of Study: This study was performed at the Instituto de Ensino e Pesquisa do Hospital Sírio-Libanês, São Paulo, Brazil, between January 2012 and December 2012. Methodology: Two sexually mature female minipigBR pigs underwent two laparotomies (each animal). The first laparotomy was performed to implant two fragments of autologous endometrium in the rectal wall. The second one was performed thirty days later to visualize, measure and obtain tissue of the site of the implants for histopathology study. A TRUS study was performed prior to the second surgery. The Institution’s Animal Utilization Study Committee approved the study. Results: In the first laparotomy a 5-cm segment of right uterine horn was resected. The endometrium was separated from the myometrium through sub-endometrial saline injection. Two endometrial fragments (1.0 x 2.0 cm) were dissected and sutured in the intra peritoneal anterior rectal wall of the animals. Thirty days later, all implants were identified during preoperative TRUS. “En-bloc” resection of the intestinal segment with the implants was performed during the second surgery. The autologous implants of endometrium invaded the muscular layer in one of the two animals. Conclusion: We demonstrated that the creation of an animal model of deep infiltrating endometriosis with intestinal involvement is feasible through a simple surgical technique. We believe that this model can be applied in experimental and clinical studies but further studies are necessary to refine the technique.
publishDate 2015
dc.date.none.fl_str_mv 2015
2016-04-01T18:42:41Z
2016-04-01T18:42:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.9734/bjast/2015/13615
British Journal of Applied Science and Technology, v. 7, n. 5, p. 449-455, 2015.
2231-0843
http://hdl.handle.net/11449/136812
10.9734/bjast/2015/13615
ISSN2231-0843-2015-07-05-449-455.pdf
9526345156590477
9526345156590477
9526345156590477
url http://dx.doi.org/10.9734/bjast/2015/13615
http://hdl.handle.net/11449/136812
identifier_str_mv British Journal of Applied Science and Technology, v. 7, n. 5, p. 449-455, 2015.
2231-0843
10.9734/bjast/2015/13615
ISSN2231-0843-2015-07-05-449-455.pdf
9526345156590477
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv British Journal of Applied Science and Technology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 449-455
application/pdf
dc.source.none.fl_str_mv Currículo Lattes
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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