Mutagenicity of a novel 2-phenylbenzotriazole (non-chlorinated 2-phenylbenzotriazole-9) in mice
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/em.22463 http://hdl.handle.net/11449/229525 |
Resumo: | Dinitrophenylazo dyes can form 2-phenylbenzotriazoles (PBTAs) in the textile dyeing process upon the addition of chemical reducing agents. Some dinitrophenylazo dyes, as well as their respective reduced (non-chlorinated) and chlorinated PBTAs, are now found in rivers owing to wastewater from textile plants. This study aimed to investigate the genotoxicity of a new PBTA derived from C.I. Disperse Violet 93 azo dye, namely non-Cl PBTA-9. Primary DNA damage in the blood, liver, and colon cells, micronucleated cells in the bone marrow, and gene expression (NAT2, CYP1A1, TRP53, and CDKN1A) in liver cells were observed in mice, at acute oral exposure (gavage) doses of 5, 50, and 500 μg/kg body weight (b.w.). The non-chlorinated PBTA-9 caused DNA damage in the blood and liver (at 500 μg/kg b.w.) and in colon cells (at 5, 50, and 500 μg/kg), and increased the frequency of micronucleated cells in the bone marrow (at 5 and 50 μg/kg). No histological alterations or gene expression changes were observed. In conclusion, in vivo exposure to non-chlorinated PBTA-9 induced genetic damage in various rodent tissues, corroborating results previously obtained from the Ames test. Because this compound has been detected in rivers, exposure to humans and biota is a major concern. |
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Mutagenicity of a novel 2-phenylbenzotriazole (non-chlorinated 2-phenylbenzotriazole-9) in mice2-phenylbenzotriazoleenvironmental mutagenesistextile dyewater pollutionDinitrophenylazo dyes can form 2-phenylbenzotriazoles (PBTAs) in the textile dyeing process upon the addition of chemical reducing agents. Some dinitrophenylazo dyes, as well as their respective reduced (non-chlorinated) and chlorinated PBTAs, are now found in rivers owing to wastewater from textile plants. This study aimed to investigate the genotoxicity of a new PBTA derived from C.I. Disperse Violet 93 azo dye, namely non-Cl PBTA-9. Primary DNA damage in the blood, liver, and colon cells, micronucleated cells in the bone marrow, and gene expression (NAT2, CYP1A1, TRP53, and CDKN1A) in liver cells were observed in mice, at acute oral exposure (gavage) doses of 5, 50, and 500 μg/kg body weight (b.w.). The non-chlorinated PBTA-9 caused DNA damage in the blood and liver (at 500 μg/kg b.w.) and in colon cells (at 5, 50, and 500 μg/kg), and increased the frequency of micronucleated cells in the bone marrow (at 5 and 50 μg/kg). No histological alterations or gene expression changes were observed. In conclusion, in vivo exposure to non-chlorinated PBTA-9 induced genetic damage in various rodent tissues, corroborating results previously obtained from the Ames test. Because this compound has been detected in rivers, exposure to humans and biota is a major concern.Department of Pathology Medical School São Paulo State University (UNESP)National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Institute of ChemistrySchool of Technology State University of Campinas (UNICAMP)School of Veterinary and Zootechnics São Paulo State University (UNESP)Department of Pathology Medical School São Paulo State University (UNESP)School of Veterinary and Zootechnics São Paulo State University (UNESP)Universidade Estadual Paulista (UNESP)Institute of ChemistryUniversidade Estadual de Campinas (UNICAMP)Rodrigues Tanamachi, Amanda [UNESP]Fernandes, Fábio Henrique [UNESP]de Souza Vendemiatti, Josiane AparecidaPrediger, PatríciaCamparotto, Natália GabrieleSousa Rocha, Noeme [UNESP]Aragão Umbuzeiro, GiselaFávero Salvadori, Daisy Maria [UNESP]2022-04-29T08:33:05Z2022-04-29T08:33:05Z2021-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article471-477http://dx.doi.org/10.1002/em.22463Environmental and Molecular Mutagenesis, v. 62, n. 8, p. 471-477, 2021.1098-22800893-6692http://hdl.handle.net/11449/22952510.1002/em.224632-s2.0-85114939151Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental and Molecular Mutagenesisinfo:eu-repo/semantics/openAccess2022-04-29T08:33:05Zoai:repositorio.unesp.br:11449/229525Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:33:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mutagenicity of a novel 2-phenylbenzotriazole (non-chlorinated 2-phenylbenzotriazole-9) in mice |
title |
Mutagenicity of a novel 2-phenylbenzotriazole (non-chlorinated 2-phenylbenzotriazole-9) in mice |
spellingShingle |
Mutagenicity of a novel 2-phenylbenzotriazole (non-chlorinated 2-phenylbenzotriazole-9) in mice Rodrigues Tanamachi, Amanda [UNESP] 2-phenylbenzotriazole environmental mutagenesis textile dye water pollution |
title_short |
Mutagenicity of a novel 2-phenylbenzotriazole (non-chlorinated 2-phenylbenzotriazole-9) in mice |
title_full |
Mutagenicity of a novel 2-phenylbenzotriazole (non-chlorinated 2-phenylbenzotriazole-9) in mice |
title_fullStr |
Mutagenicity of a novel 2-phenylbenzotriazole (non-chlorinated 2-phenylbenzotriazole-9) in mice |
title_full_unstemmed |
Mutagenicity of a novel 2-phenylbenzotriazole (non-chlorinated 2-phenylbenzotriazole-9) in mice |
title_sort |
Mutagenicity of a novel 2-phenylbenzotriazole (non-chlorinated 2-phenylbenzotriazole-9) in mice |
author |
Rodrigues Tanamachi, Amanda [UNESP] |
author_facet |
Rodrigues Tanamachi, Amanda [UNESP] Fernandes, Fábio Henrique [UNESP] de Souza Vendemiatti, Josiane Aparecida Prediger, Patrícia Camparotto, Natália Gabriele Sousa Rocha, Noeme [UNESP] Aragão Umbuzeiro, Gisela Fávero Salvadori, Daisy Maria [UNESP] |
author_role |
author |
author2 |
Fernandes, Fábio Henrique [UNESP] de Souza Vendemiatti, Josiane Aparecida Prediger, Patrícia Camparotto, Natália Gabriele Sousa Rocha, Noeme [UNESP] Aragão Umbuzeiro, Gisela Fávero Salvadori, Daisy Maria [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Institute of Chemistry Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Rodrigues Tanamachi, Amanda [UNESP] Fernandes, Fábio Henrique [UNESP] de Souza Vendemiatti, Josiane Aparecida Prediger, Patrícia Camparotto, Natália Gabriele Sousa Rocha, Noeme [UNESP] Aragão Umbuzeiro, Gisela Fávero Salvadori, Daisy Maria [UNESP] |
dc.subject.por.fl_str_mv |
2-phenylbenzotriazole environmental mutagenesis textile dye water pollution |
topic |
2-phenylbenzotriazole environmental mutagenesis textile dye water pollution |
description |
Dinitrophenylazo dyes can form 2-phenylbenzotriazoles (PBTAs) in the textile dyeing process upon the addition of chemical reducing agents. Some dinitrophenylazo dyes, as well as their respective reduced (non-chlorinated) and chlorinated PBTAs, are now found in rivers owing to wastewater from textile plants. This study aimed to investigate the genotoxicity of a new PBTA derived from C.I. Disperse Violet 93 azo dye, namely non-Cl PBTA-9. Primary DNA damage in the blood, liver, and colon cells, micronucleated cells in the bone marrow, and gene expression (NAT2, CYP1A1, TRP53, and CDKN1A) in liver cells were observed in mice, at acute oral exposure (gavage) doses of 5, 50, and 500 μg/kg body weight (b.w.). The non-chlorinated PBTA-9 caused DNA damage in the blood and liver (at 500 μg/kg b.w.) and in colon cells (at 5, 50, and 500 μg/kg), and increased the frequency of micronucleated cells in the bone marrow (at 5 and 50 μg/kg). No histological alterations or gene expression changes were observed. In conclusion, in vivo exposure to non-chlorinated PBTA-9 induced genetic damage in various rodent tissues, corroborating results previously obtained from the Ames test. Because this compound has been detected in rivers, exposure to humans and biota is a major concern. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-01 2022-04-29T08:33:05Z 2022-04-29T08:33:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/em.22463 Environmental and Molecular Mutagenesis, v. 62, n. 8, p. 471-477, 2021. 1098-2280 0893-6692 http://hdl.handle.net/11449/229525 10.1002/em.22463 2-s2.0-85114939151 |
url |
http://dx.doi.org/10.1002/em.22463 http://hdl.handle.net/11449/229525 |
identifier_str_mv |
Environmental and Molecular Mutagenesis, v. 62, n. 8, p. 471-477, 2021. 1098-2280 0893-6692 10.1002/em.22463 2-s2.0-85114939151 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental and Molecular Mutagenesis |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
471-477 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1803649581029785600 |