The role of detoxification enzymes in the susceptibility of Brevipalpus californicus exposed to acaricide and insecticide mixtures
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pestbp.2021.104855 http://hdl.handle.net/11449/206188 |
Resumo: | The intense spraying of pesticides to control different arthropod pests has resulted in negative side effects for the management of pests. It was previously discovered that exposure to non-acaricidal insecticides alone or in a mixture, results in lower efficiency of the acaricide spirodiclofen used for Brevipalpus spp. control. We investigate here whether the induced expression of detoxification enzymes by non-lethal insecticides may antagonize spirodiclofen toxicity. Brevipalpus californicus mites exposed to the insecticide phosmet alone or in combination with spirodiclofen showed increased activity of P450 monooxygenases (P450s). No antagonistic effects in mite mortality were observed by the combination of phosmet and spirodiclofen. On the other hand, mites exposed to the insecticide imidacloprid alone or in combination with spirodiclofen showed an increase in the activity of P450s, carboxylcholinesterases (CCE), and glutathione-S-transferases (GST). An antagonistic effect on mite mortality was observed when mites were exposed to the LC25 of spirodiclofen combined with the field rate treatment of imidacloprid. The addition of PBO (a P450 monooxygenase inhibitor) to the mixture of spirodiclofen and imidacloprid resulted in a synergistic effect over mite mortality but the addition of DEM (a GST inhibitor) resulted in an antagonist effect. Taken together, this study showed that the combination of imidacloprid with spirodiclofen is antagonistic for the control of B. californicus, and this results from the induction of detoxification enzymes, such as P450s, CCE, and GST. The use of inhibitors highlights the role of these enzymes in the antagonism of the mixture. |
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The role of detoxification enzymes in the susceptibility of Brevipalpus californicus exposed to acaricide and insecticide mixturesAntagonismEsterasesGSTMetabolismMonooxygenasesSynergismThe intense spraying of pesticides to control different arthropod pests has resulted in negative side effects for the management of pests. It was previously discovered that exposure to non-acaricidal insecticides alone or in a mixture, results in lower efficiency of the acaricide spirodiclofen used for Brevipalpus spp. control. We investigate here whether the induced expression of detoxification enzymes by non-lethal insecticides may antagonize spirodiclofen toxicity. Brevipalpus californicus mites exposed to the insecticide phosmet alone or in combination with spirodiclofen showed increased activity of P450 monooxygenases (P450s). No antagonistic effects in mite mortality were observed by the combination of phosmet and spirodiclofen. On the other hand, mites exposed to the insecticide imidacloprid alone or in combination with spirodiclofen showed an increase in the activity of P450s, carboxylcholinesterases (CCE), and glutathione-S-transferases (GST). An antagonistic effect on mite mortality was observed when mites were exposed to the LC25 of spirodiclofen combined with the field rate treatment of imidacloprid. The addition of PBO (a P450 monooxygenase inhibitor) to the mixture of spirodiclofen and imidacloprid resulted in a synergistic effect over mite mortality but the addition of DEM (a GST inhibitor) resulted in an antagonist effect. Taken together, this study showed that the combination of imidacloprid with spirodiclofen is antagonistic for the control of B. californicus, and this results from the induction of detoxification enzymes, such as P450s, CCE, and GST. The use of inhibitors highlights the role of these enzymes in the antagonism of the mixture.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Agricultural Sciences São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, Via de Acesso Prof. Paulo Donato Castellane S/N. Zip code: 14Laboratory of Agrozoology Department of Crop Protection Faculty of Bioscience Engineering Ghent University, Coupure Links 653Department of Agricultural Sciences São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, Via de Acesso Prof. Paulo Donato Castellane S/N. Zip code: 14FAPESP: #2019/08384-9FAPESP: #301492/2018-2Universidade Estadual Paulista (Unesp)Ghent UniversityDella Vechia, Jaqueline F. [UNESP]Van Leeuwen, ThomasRossi, Guilherme D. [UNESP]Andrade, Daniel J. [UNESP]2021-06-25T10:28:02Z2021-06-25T10:28:02Z2021-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.pestbp.2021.104855Pesticide Biochemistry and Physiology, v. 175.1095-99390048-3575http://hdl.handle.net/11449/20618810.1016/j.pestbp.2021.1048552-s2.0-85104087391Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesticide Biochemistry and Physiologyinfo:eu-repo/semantics/openAccess2021-10-22T22:17:04Zoai:repositorio.unesp.br:11449/206188Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:09:17.315907Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The role of detoxification enzymes in the susceptibility of Brevipalpus californicus exposed to acaricide and insecticide mixtures |
title |
The role of detoxification enzymes in the susceptibility of Brevipalpus californicus exposed to acaricide and insecticide mixtures |
spellingShingle |
The role of detoxification enzymes in the susceptibility of Brevipalpus californicus exposed to acaricide and insecticide mixtures Della Vechia, Jaqueline F. [UNESP] Antagonism Esterases GST Metabolism Monooxygenases Synergism |
title_short |
The role of detoxification enzymes in the susceptibility of Brevipalpus californicus exposed to acaricide and insecticide mixtures |
title_full |
The role of detoxification enzymes in the susceptibility of Brevipalpus californicus exposed to acaricide and insecticide mixtures |
title_fullStr |
The role of detoxification enzymes in the susceptibility of Brevipalpus californicus exposed to acaricide and insecticide mixtures |
title_full_unstemmed |
The role of detoxification enzymes in the susceptibility of Brevipalpus californicus exposed to acaricide and insecticide mixtures |
title_sort |
The role of detoxification enzymes in the susceptibility of Brevipalpus californicus exposed to acaricide and insecticide mixtures |
author |
Della Vechia, Jaqueline F. [UNESP] |
author_facet |
Della Vechia, Jaqueline F. [UNESP] Van Leeuwen, Thomas Rossi, Guilherme D. [UNESP] Andrade, Daniel J. [UNESP] |
author_role |
author |
author2 |
Van Leeuwen, Thomas Rossi, Guilherme D. [UNESP] Andrade, Daniel J. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Ghent University |
dc.contributor.author.fl_str_mv |
Della Vechia, Jaqueline F. [UNESP] Van Leeuwen, Thomas Rossi, Guilherme D. [UNESP] Andrade, Daniel J. [UNESP] |
dc.subject.por.fl_str_mv |
Antagonism Esterases GST Metabolism Monooxygenases Synergism |
topic |
Antagonism Esterases GST Metabolism Monooxygenases Synergism |
description |
The intense spraying of pesticides to control different arthropod pests has resulted in negative side effects for the management of pests. It was previously discovered that exposure to non-acaricidal insecticides alone or in a mixture, results in lower efficiency of the acaricide spirodiclofen used for Brevipalpus spp. control. We investigate here whether the induced expression of detoxification enzymes by non-lethal insecticides may antagonize spirodiclofen toxicity. Brevipalpus californicus mites exposed to the insecticide phosmet alone or in combination with spirodiclofen showed increased activity of P450 monooxygenases (P450s). No antagonistic effects in mite mortality were observed by the combination of phosmet and spirodiclofen. On the other hand, mites exposed to the insecticide imidacloprid alone or in combination with spirodiclofen showed an increase in the activity of P450s, carboxylcholinesterases (CCE), and glutathione-S-transferases (GST). An antagonistic effect on mite mortality was observed when mites were exposed to the LC25 of spirodiclofen combined with the field rate treatment of imidacloprid. The addition of PBO (a P450 monooxygenase inhibitor) to the mixture of spirodiclofen and imidacloprid resulted in a synergistic effect over mite mortality but the addition of DEM (a GST inhibitor) resulted in an antagonist effect. Taken together, this study showed that the combination of imidacloprid with spirodiclofen is antagonistic for the control of B. californicus, and this results from the induction of detoxification enzymes, such as P450s, CCE, and GST. The use of inhibitors highlights the role of these enzymes in the antagonism of the mixture. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:28:02Z 2021-06-25T10:28:02Z 2021-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pestbp.2021.104855 Pesticide Biochemistry and Physiology, v. 175. 1095-9939 0048-3575 http://hdl.handle.net/11449/206188 10.1016/j.pestbp.2021.104855 2-s2.0-85104087391 |
url |
http://dx.doi.org/10.1016/j.pestbp.2021.104855 http://hdl.handle.net/11449/206188 |
identifier_str_mv |
Pesticide Biochemistry and Physiology, v. 175. 1095-9939 0048-3575 10.1016/j.pestbp.2021.104855 2-s2.0-85104087391 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pesticide Biochemistry and Physiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129590373122048 |