Molecular detection of vector-borne agents in ectoparasites and reptiles from Brazil

Detalhes bibliográficos
Autor(a) principal: Mendoza-Roldan, Jairo Alfonso
Data de Publicação: 2021
Outros Autores: Ribeiro, Stephany Rocha, Castilho-Onofrio, Valeria, Marcili, Arlei, Simonato, Bruna Borghi, Latrofa, Maria Stefania, Benelli, Giovanni, Otranto, Domenico, Barros-Battesti, Darci Moraes [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.ttbdis.2020.101585
http://hdl.handle.net/11449/205395
Resumo: Trombidiformes and Mesostigmata mites, as well as Ixodida ticks, infest ectothermic tetrapods worldwide, potentially acting as vectors of bacteria, viruses and protozoa. The relationship among ectoparasites, transmitted pathogenic agents (e.g., Borrelia spp., Coxiella spp., Hepatozoon spp., and Rickettsia spp.) and ectothermic hosts has been scarcely investigated. This research focuses on a large collection of Brazilian herpetofauna screened for the presence of arthropod ectoparasites and vector-borne microbial agents. Reptiles (n = 121) and amphibians (n = 49) from various locations were infested by ectoparasites. Following genomic extraction, microbial agents were detected in 81 % of the Acari (i.e. n = 113 mites and n = 26 ticks). None of the mites, ticks and tissues from amphibians yielded positive results for any of the screened agents. Blood was collected from reptiles and processed through blood cytology and molecular analyses (n = 48). Of those, six snakes (12.5 %) showed intraerythrocytic alterations compatible with Hepatozoon spp. gamonts and Iridovirus inclusions. Hepatozoon spp. similar to Hepatozoon ayorgbor and Hepatozoon musa were molecularly identified from seven hosts, two mite and two tick species. Rickettsia spp. (e.g., Rickettsia amblyommatis, Rickettsia bellii-like, Rickettsia sp.) were detected molecularly from four mite species and Amblyomma rotundatum ticks. Phylogenetic analyses confirmed the molecular identification of the above-mentioned microbial agents of mites and ticks related to snakes and lizards. Overall, our findings highlighted that the Brazilian herpetofauna and its ectoparasites harbour potentially pathogenic agents, particularly from the northern and south-eastern regions. The detection of several species of spotted fever group Rickettsia pointed out the potential role of ectothermic hosts and related arthropod ectoparasites in the epidemiological cycle of these bacteria in Brazil.
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spelling Molecular detection of vector-borne agents in ectoparasites and reptiles from BrazilHepatozoon spp.LizardsMitesRickettsia spp.SnakesTicksVector-borne diseasesTrombidiformes and Mesostigmata mites, as well as Ixodida ticks, infest ectothermic tetrapods worldwide, potentially acting as vectors of bacteria, viruses and protozoa. The relationship among ectoparasites, transmitted pathogenic agents (e.g., Borrelia spp., Coxiella spp., Hepatozoon spp., and Rickettsia spp.) and ectothermic hosts has been scarcely investigated. This research focuses on a large collection of Brazilian herpetofauna screened for the presence of arthropod ectoparasites and vector-borne microbial agents. Reptiles (n = 121) and amphibians (n = 49) from various locations were infested by ectoparasites. Following genomic extraction, microbial agents were detected in 81 % of the Acari (i.e. n = 113 mites and n = 26 ticks). None of the mites, ticks and tissues from amphibians yielded positive results for any of the screened agents. Blood was collected from reptiles and processed through blood cytology and molecular analyses (n = 48). Of those, six snakes (12.5 %) showed intraerythrocytic alterations compatible with Hepatozoon spp. gamonts and Iridovirus inclusions. Hepatozoon spp. similar to Hepatozoon ayorgbor and Hepatozoon musa were molecularly identified from seven hosts, two mite and two tick species. Rickettsia spp. (e.g., Rickettsia amblyommatis, Rickettsia bellii-like, Rickettsia sp.) were detected molecularly from four mite species and Amblyomma rotundatum ticks. Phylogenetic analyses confirmed the molecular identification of the above-mentioned microbial agents of mites and ticks related to snakes and lizards. Overall, our findings highlighted that the Brazilian herpetofauna and its ectoparasites harbour potentially pathogenic agents, particularly from the northern and south-eastern regions. The detection of several species of spotted fever group Rickettsia pointed out the potential role of ectothermic hosts and related arthropod ectoparasites in the epidemiological cycle of these bacteria in Brazil.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Veterinary Medicine University of BariFaculty of Veterinary Medicine University of São PauloZoological Collections Laboratory Butantan InstituteMaster's Program in Veterinary Medicine and Animal Welfare and Doctoral Program in One Health Santo Amaro UniversityDeparment of Veterinary Medicine Anhembi Morumbi University Vila TramontanoDepartment of Agriculture Food and Environment University of Pisa, via Del Borghetto 80Department of Veterinary Pathology Universidade Estadual Paulista Julio De Mesquita Filho (UNESP)Department of Pathobiology Faculty of Veterinary Science Bu-Ali Sina University Felestin Sq.Department of Veterinary Pathology Universidade Estadual Paulista Julio De Mesquita Filho (UNESP)CNPq: 142409/2015-4FAPESP: 2010/51875-9FAPESP: 2017/12910-2CNPq: 440639/2015-8CNPq: 454907/2014-1University of BariUniversidade de São Paulo (USP)Butantan InstituteSanto Amaro UniversityAnhembi Morumbi University Vila TramontanoUniversity of PisaUniversidade Estadual Paulista (Unesp)Felestin Sq.Mendoza-Roldan, Jairo AlfonsoRibeiro, Stephany RochaCastilho-Onofrio, ValeriaMarcili, ArleiSimonato, Bruna BorghiLatrofa, Maria StefaniaBenelli, GiovanniOtranto, DomenicoBarros-Battesti, Darci Moraes [UNESP]2021-06-25T10:14:39Z2021-06-25T10:14:39Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.ttbdis.2020.101585Ticks and Tick-borne Diseases, v. 12, n. 1, 2021.1877-96031877-959Xhttp://hdl.handle.net/11449/20539510.1016/j.ttbdis.2020.1015852-s2.0-85094212475Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTicks and Tick-borne Diseasesinfo:eu-repo/semantics/openAccess2021-10-23T12:40:01Zoai:repositorio.unesp.br:11449/205395Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:06:11.343275Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular detection of vector-borne agents in ectoparasites and reptiles from Brazil
title Molecular detection of vector-borne agents in ectoparasites and reptiles from Brazil
spellingShingle Molecular detection of vector-borne agents in ectoparasites and reptiles from Brazil
Mendoza-Roldan, Jairo Alfonso
Hepatozoon spp.
Lizards
Mites
Rickettsia spp.
Snakes
Ticks
Vector-borne diseases
title_short Molecular detection of vector-borne agents in ectoparasites and reptiles from Brazil
title_full Molecular detection of vector-borne agents in ectoparasites and reptiles from Brazil
title_fullStr Molecular detection of vector-borne agents in ectoparasites and reptiles from Brazil
title_full_unstemmed Molecular detection of vector-borne agents in ectoparasites and reptiles from Brazil
title_sort Molecular detection of vector-borne agents in ectoparasites and reptiles from Brazil
author Mendoza-Roldan, Jairo Alfonso
author_facet Mendoza-Roldan, Jairo Alfonso
Ribeiro, Stephany Rocha
Castilho-Onofrio, Valeria
Marcili, Arlei
Simonato, Bruna Borghi
Latrofa, Maria Stefania
Benelli, Giovanni
Otranto, Domenico
Barros-Battesti, Darci Moraes [UNESP]
author_role author
author2 Ribeiro, Stephany Rocha
Castilho-Onofrio, Valeria
Marcili, Arlei
Simonato, Bruna Borghi
Latrofa, Maria Stefania
Benelli, Giovanni
Otranto, Domenico
Barros-Battesti, Darci Moraes [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv University of Bari
Universidade de São Paulo (USP)
Butantan Institute
Santo Amaro University
Anhembi Morumbi University Vila Tramontano
University of Pisa
Universidade Estadual Paulista (Unesp)
Felestin Sq.
dc.contributor.author.fl_str_mv Mendoza-Roldan, Jairo Alfonso
Ribeiro, Stephany Rocha
Castilho-Onofrio, Valeria
Marcili, Arlei
Simonato, Bruna Borghi
Latrofa, Maria Stefania
Benelli, Giovanni
Otranto, Domenico
Barros-Battesti, Darci Moraes [UNESP]
dc.subject.por.fl_str_mv Hepatozoon spp.
Lizards
Mites
Rickettsia spp.
Snakes
Ticks
Vector-borne diseases
topic Hepatozoon spp.
Lizards
Mites
Rickettsia spp.
Snakes
Ticks
Vector-borne diseases
description Trombidiformes and Mesostigmata mites, as well as Ixodida ticks, infest ectothermic tetrapods worldwide, potentially acting as vectors of bacteria, viruses and protozoa. The relationship among ectoparasites, transmitted pathogenic agents (e.g., Borrelia spp., Coxiella spp., Hepatozoon spp., and Rickettsia spp.) and ectothermic hosts has been scarcely investigated. This research focuses on a large collection of Brazilian herpetofauna screened for the presence of arthropod ectoparasites and vector-borne microbial agents. Reptiles (n = 121) and amphibians (n = 49) from various locations were infested by ectoparasites. Following genomic extraction, microbial agents were detected in 81 % of the Acari (i.e. n = 113 mites and n = 26 ticks). None of the mites, ticks and tissues from amphibians yielded positive results for any of the screened agents. Blood was collected from reptiles and processed through blood cytology and molecular analyses (n = 48). Of those, six snakes (12.5 %) showed intraerythrocytic alterations compatible with Hepatozoon spp. gamonts and Iridovirus inclusions. Hepatozoon spp. similar to Hepatozoon ayorgbor and Hepatozoon musa were molecularly identified from seven hosts, two mite and two tick species. Rickettsia spp. (e.g., Rickettsia amblyommatis, Rickettsia bellii-like, Rickettsia sp.) were detected molecularly from four mite species and Amblyomma rotundatum ticks. Phylogenetic analyses confirmed the molecular identification of the above-mentioned microbial agents of mites and ticks related to snakes and lizards. Overall, our findings highlighted that the Brazilian herpetofauna and its ectoparasites harbour potentially pathogenic agents, particularly from the northern and south-eastern regions. The detection of several species of spotted fever group Rickettsia pointed out the potential role of ectothermic hosts and related arthropod ectoparasites in the epidemiological cycle of these bacteria in Brazil.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:14:39Z
2021-06-25T10:14:39Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.ttbdis.2020.101585
Ticks and Tick-borne Diseases, v. 12, n. 1, 2021.
1877-9603
1877-959X
http://hdl.handle.net/11449/205395
10.1016/j.ttbdis.2020.101585
2-s2.0-85094212475
url http://dx.doi.org/10.1016/j.ttbdis.2020.101585
http://hdl.handle.net/11449/205395
identifier_str_mv Ticks and Tick-borne Diseases, v. 12, n. 1, 2021.
1877-9603
1877-959X
10.1016/j.ttbdis.2020.101585
2-s2.0-85094212475
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ticks and Tick-borne Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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