Prevalence and distribution of Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) in Achatina fulica (Mollusca, Gastropoda) in Baixada Santista, Sao Paulo, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0037-8682-0316-2016 http://hdl.handle.net/11449/162578 |
Resumo: | Introduction: Angiostrongylus cantonensis causes eosinophilic meningoencephalitis in humans. Worldwide expansion of this nematode is linked to the dispersion of their hosts. This study aimed to determine the prevalence of A. cantonensis infection in Achatina fulica in the nine municipalities that make up Baixada Santista, Sao Paulo, Brazil. Methods: Angiostrongylus cantonensis larvae were analyzed using optical microscopy. We performed polymerase chain reaction and restriction fragment length polymorphism using restriction endonuclease ClaI, directed to the internal transcribed spacer region 2 of A. cantonensis larval DNA. Results: Of the 540 snails analyzed, 117 (21.7%) were infected by A. cantonensis. For morphological and morphometric analyses, 60 larvae were used. Second-stage larvae were, on average, 358.2 mu m long and 26.4 mu m wide, while third-stage larvae were, on average, 450 mu m long and 21.12 mu m wide. The tails of the larvae ended in a fine tip. Conclusions: All municipalities comprising Baixada Santista had A. fulica that were naturally infected with A. cantonensis. All of the observed characteristics were typical of the species. |
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Prevalence and distribution of Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) in Achatina fulica (Mollusca, Gastropoda) in Baixada Santista, Sao Paulo, BrazilRat lungwormGiant African snailEosinophilic meningitisNematodeEmerging parasitosisIntroduction: Angiostrongylus cantonensis causes eosinophilic meningoencephalitis in humans. Worldwide expansion of this nematode is linked to the dispersion of their hosts. This study aimed to determine the prevalence of A. cantonensis infection in Achatina fulica in the nine municipalities that make up Baixada Santista, Sao Paulo, Brazil. Methods: Angiostrongylus cantonensis larvae were analyzed using optical microscopy. We performed polymerase chain reaction and restriction fragment length polymorphism using restriction endonuclease ClaI, directed to the internal transcribed spacer region 2 of A. cantonensis larval DNA. Results: Of the 540 snails analyzed, 117 (21.7%) were infected by A. cantonensis. For morphological and morphometric analyses, 60 larvae were used. Second-stage larvae were, on average, 358.2 mu m long and 26.4 mu m wide, while third-stage larvae were, on average, 450 mu m long and 21.12 mu m wide. The tails of the larvae ended in a fine tip. Conclusions: All municipalities comprising Baixada Santista had A. fulica that were naturally infected with A. cantonensis. All of the observed characteristics were typical of the species.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Lab Parasitol Animais Silvestres, Dept Parasitol, Botucatu, SP, BrazilUniv Estadual Paulista, Lab Moluscos, Sao Vicente, SP, BrazilUniv Estadual Campinas, Lab Malacol, Dept Biol Anim, Campinas, SP, BrazilFundacao Oswaldo Cruz, Lab Helmintol & Malacol Med, Ctr Pesquisas Rene Rachou, Belo Horizonte, MG, BrazilUniv Estadual Paulista, Lab Parasitol Animais Silvestres, Dept Parasitol, Botucatu, SP, BrazilUniv Estadual Paulista, Lab Moluscos, Sao Vicente, SP, BrazilFAPESP: 2011/05893-8Soc Brasileira Medicina TropicalUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Fundacao Oswaldo CruzGuerino, Laura Rocha [UNESP]Pecora, Iracy Lea [UNESP]Miranda, Marcel SabinoAguiar-Silva, CryslaineCarvalho, Omar dos SantosCaldeira, Roberta LimaSilva, Reinaldo Jose da [UNESP]2018-11-26T17:21:00Z2018-11-26T17:21:00Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article92-98application/pdfhttp://dx.doi.org/10.1590/0037-8682-0316-2016Revista Da Sociedade Brasileira De Medicina Tropical. Brasilia: Soc Brasileira Medicina Tropical, v. 50, n. 1, p. 92-98, 2017.0037-8682http://hdl.handle.net/11449/16257810.1590/0037-8682-0316-2016S0037-86822017000100092WOS:000396497600014S0037-86822017000100092.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Da Sociedade Brasileira De Medicina Tropical0,658info:eu-repo/semantics/openAccess2024-01-10T06:23:18Zoai:repositorio.unesp.br:11449/162578Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:35:02.942109Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Prevalence and distribution of Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) in Achatina fulica (Mollusca, Gastropoda) in Baixada Santista, Sao Paulo, Brazil |
title |
Prevalence and distribution of Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) in Achatina fulica (Mollusca, Gastropoda) in Baixada Santista, Sao Paulo, Brazil |
spellingShingle |
Prevalence and distribution of Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) in Achatina fulica (Mollusca, Gastropoda) in Baixada Santista, Sao Paulo, Brazil Guerino, Laura Rocha [UNESP] Rat lungworm Giant African snail Eosinophilic meningitis Nematode Emerging parasitosis |
title_short |
Prevalence and distribution of Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) in Achatina fulica (Mollusca, Gastropoda) in Baixada Santista, Sao Paulo, Brazil |
title_full |
Prevalence and distribution of Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) in Achatina fulica (Mollusca, Gastropoda) in Baixada Santista, Sao Paulo, Brazil |
title_fullStr |
Prevalence and distribution of Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) in Achatina fulica (Mollusca, Gastropoda) in Baixada Santista, Sao Paulo, Brazil |
title_full_unstemmed |
Prevalence and distribution of Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) in Achatina fulica (Mollusca, Gastropoda) in Baixada Santista, Sao Paulo, Brazil |
title_sort |
Prevalence and distribution of Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) in Achatina fulica (Mollusca, Gastropoda) in Baixada Santista, Sao Paulo, Brazil |
author |
Guerino, Laura Rocha [UNESP] |
author_facet |
Guerino, Laura Rocha [UNESP] Pecora, Iracy Lea [UNESP] Miranda, Marcel Sabino Aguiar-Silva, Cryslaine Carvalho, Omar dos Santos Caldeira, Roberta Lima Silva, Reinaldo Jose da [UNESP] |
author_role |
author |
author2 |
Pecora, Iracy Lea [UNESP] Miranda, Marcel Sabino Aguiar-Silva, Cryslaine Carvalho, Omar dos Santos Caldeira, Roberta Lima Silva, Reinaldo Jose da [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Fundacao Oswaldo Cruz |
dc.contributor.author.fl_str_mv |
Guerino, Laura Rocha [UNESP] Pecora, Iracy Lea [UNESP] Miranda, Marcel Sabino Aguiar-Silva, Cryslaine Carvalho, Omar dos Santos Caldeira, Roberta Lima Silva, Reinaldo Jose da [UNESP] |
dc.subject.por.fl_str_mv |
Rat lungworm Giant African snail Eosinophilic meningitis Nematode Emerging parasitosis |
topic |
Rat lungworm Giant African snail Eosinophilic meningitis Nematode Emerging parasitosis |
description |
Introduction: Angiostrongylus cantonensis causes eosinophilic meningoencephalitis in humans. Worldwide expansion of this nematode is linked to the dispersion of their hosts. This study aimed to determine the prevalence of A. cantonensis infection in Achatina fulica in the nine municipalities that make up Baixada Santista, Sao Paulo, Brazil. Methods: Angiostrongylus cantonensis larvae were analyzed using optical microscopy. We performed polymerase chain reaction and restriction fragment length polymorphism using restriction endonuclease ClaI, directed to the internal transcribed spacer region 2 of A. cantonensis larval DNA. Results: Of the 540 snails analyzed, 117 (21.7%) were infected by A. cantonensis. For morphological and morphometric analyses, 60 larvae were used. Second-stage larvae were, on average, 358.2 mu m long and 26.4 mu m wide, while third-stage larvae were, on average, 450 mu m long and 21.12 mu m wide. The tails of the larvae ended in a fine tip. Conclusions: All municipalities comprising Baixada Santista had A. fulica that were naturally infected with A. cantonensis. All of the observed characteristics were typical of the species. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-11-26T17:21:00Z 2018-11-26T17:21:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0037-8682-0316-2016 Revista Da Sociedade Brasileira De Medicina Tropical. Brasilia: Soc Brasileira Medicina Tropical, v. 50, n. 1, p. 92-98, 2017. 0037-8682 http://hdl.handle.net/11449/162578 10.1590/0037-8682-0316-2016 S0037-86822017000100092 WOS:000396497600014 S0037-86822017000100092.pdf |
url |
http://dx.doi.org/10.1590/0037-8682-0316-2016 http://hdl.handle.net/11449/162578 |
identifier_str_mv |
Revista Da Sociedade Brasileira De Medicina Tropical. Brasilia: Soc Brasileira Medicina Tropical, v. 50, n. 1, p. 92-98, 2017. 0037-8682 10.1590/0037-8682-0316-2016 S0037-86822017000100092 WOS:000396497600014 S0037-86822017000100092.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Da Sociedade Brasileira De Medicina Tropical 0,658 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
92-98 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasileira Medicina Tropical |
publisher.none.fl_str_mv |
Soc Brasileira Medicina Tropical |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129440312459264 |