Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ttbdis.2017.04.011 http://hdl.handle.net/11449/178811 |
Resumo: | New genotypes of Anaplasmataceae agents have been detected in wild carnivores, birds and deer in Brazil. The present work aimed to investigate the presence of Ehrlichia and Anaplasma species in rodents sampled in Brazil. Additionally, a newly designed quantitative 5′ nuclease real-time multiplex PCR for Ehrlichia and Anaplasma spp. detection based on groEL gene amplification was designed, showing high specificity and sensitivity (10 groEL fragment copy/μL). Between 2000 and 2011, different rodent species [n = 60] were trapped in 5 Brazilian biomes. Among 458 rodent spleen samples, 0.4% (2/458) and 2.4% (11/458) were positive for Ehrlichia and Anaplasma spp., respectively. Of 458 samples, 2.0% (9/458) and 1.1% (5/458) were positive for Anaplasma sp. and Ehrlichia sp., respectively, using conventional 16S rRNA PCR assays. Maximum Likelihood phylogenetic analyse based on a small region of 16S rRNA genes positioned the Anaplasma genotypes in rodents near Anaplasma phagocytophilum or Anaplasma marginale and Anaplasma odocoilei isolates. Ehrlichia genotypes were closely related to E. canis. There was a low occurrence of Anaplasma and Ehrlichia in wild and synanthropic rodents in Brazil, suggesting the circulation of new genotypes of these agents in rodents in the studied areas. |
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Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in BrazilNew genotypes of Anaplasmataceae agents have been detected in wild carnivores, birds and deer in Brazil. The present work aimed to investigate the presence of Ehrlichia and Anaplasma species in rodents sampled in Brazil. Additionally, a newly designed quantitative 5′ nuclease real-time multiplex PCR for Ehrlichia and Anaplasma spp. detection based on groEL gene amplification was designed, showing high specificity and sensitivity (10 groEL fragment copy/μL). Between 2000 and 2011, different rodent species [n = 60] were trapped in 5 Brazilian biomes. Among 458 rodent spleen samples, 0.4% (2/458) and 2.4% (11/458) were positive for Ehrlichia and Anaplasma spp., respectively. Of 458 samples, 2.0% (9/458) and 1.1% (5/458) were positive for Anaplasma sp. and Ehrlichia sp., respectively, using conventional 16S rRNA PCR assays. Maximum Likelihood phylogenetic analyse based on a small region of 16S rRNA genes positioned the Anaplasma genotypes in rodents near Anaplasma phagocytophilum or Anaplasma marginale and Anaplasma odocoilei isolates. Ehrlichia genotypes were closely related to E. canis. There was a low occurrence of Anaplasma and Ehrlichia in wild and synanthropic rodents in Brazil, suggesting the circulation of new genotypes of these agents in rodents in the studied areas.Universidade Estadual Paulista (Unesp) Faculdade de Ciências Agrárias e Veterinárias, JaboticabalJohns Hopkins School of MedicineUniformed Services UniversityLaboratório de Hantaviroses e Rickettsioses Instituto Oswaldo Cruz/FiocruzLaboratório de Biologia de Tripanosomatídeos Instituto Oswaldo Cruz/FiocruzLaboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios Instituto Oswaldo Cruz/FiocruzUniversidade Estadual Paulista (Unesp) Faculdade de Ciências Agrárias e Veterinárias, JaboticabalUniversidade Estadual Paulista (Unesp)Johns Hopkins School of MedicineUniformed Services UniversityInstituto Oswaldo Cruz/FiocruzBenevenute, Jyan Lucas [UNESP]Dumler, John StephenOgrzewalska, MariaRoque, André Luiz RodriguesMello, Victoria Valente Califre [UNESP]de Sousa, Keyla Carstens Marques [UNESP]Gonçalves, Luiz Ricardo [UNESP]D'Andrea, Paulo Sérgiode Sampaio Lemos, Elba ReginaMachado, Rosangela Zacarias [UNESP]André, Marcos Rogério [UNESP]2018-12-11T17:32:12Z2018-12-11T17:32:12Z2017-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article646-656application/pdfhttp://dx.doi.org/10.1016/j.ttbdis.2017.04.011Ticks and Tick-borne Diseases, v. 8, n. 4, p. 646-656, 2017.1877-96031877-959Xhttp://hdl.handle.net/11449/17881110.1016/j.ttbdis.2017.04.0112-s2.0-850181598002-s2.0-85018159800.pdf9139899895580513Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTicks and Tick-borne Diseases1,421info:eu-repo/semantics/openAccess2024-06-07T13:01:45Zoai:repositorio.unesp.br:11449/178811Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:49:28.788147Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil |
title |
Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil |
spellingShingle |
Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil Benevenute, Jyan Lucas [UNESP] |
title_short |
Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil |
title_full |
Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil |
title_fullStr |
Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil |
title_full_unstemmed |
Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil |
title_sort |
Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil |
author |
Benevenute, Jyan Lucas [UNESP] |
author_facet |
Benevenute, Jyan Lucas [UNESP] Dumler, John Stephen Ogrzewalska, Maria Roque, André Luiz Rodrigues Mello, Victoria Valente Califre [UNESP] de Sousa, Keyla Carstens Marques [UNESP] Gonçalves, Luiz Ricardo [UNESP] D'Andrea, Paulo Sérgio de Sampaio Lemos, Elba Regina Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
author_role |
author |
author2 |
Dumler, John Stephen Ogrzewalska, Maria Roque, André Luiz Rodrigues Mello, Victoria Valente Califre [UNESP] de Sousa, Keyla Carstens Marques [UNESP] Gonçalves, Luiz Ricardo [UNESP] D'Andrea, Paulo Sérgio de Sampaio Lemos, Elba Regina Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Johns Hopkins School of Medicine Uniformed Services University Instituto Oswaldo Cruz/Fiocruz |
dc.contributor.author.fl_str_mv |
Benevenute, Jyan Lucas [UNESP] Dumler, John Stephen Ogrzewalska, Maria Roque, André Luiz Rodrigues Mello, Victoria Valente Califre [UNESP] de Sousa, Keyla Carstens Marques [UNESP] Gonçalves, Luiz Ricardo [UNESP] D'Andrea, Paulo Sérgio de Sampaio Lemos, Elba Regina Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
description |
New genotypes of Anaplasmataceae agents have been detected in wild carnivores, birds and deer in Brazil. The present work aimed to investigate the presence of Ehrlichia and Anaplasma species in rodents sampled in Brazil. Additionally, a newly designed quantitative 5′ nuclease real-time multiplex PCR for Ehrlichia and Anaplasma spp. detection based on groEL gene amplification was designed, showing high specificity and sensitivity (10 groEL fragment copy/μL). Between 2000 and 2011, different rodent species [n = 60] were trapped in 5 Brazilian biomes. Among 458 rodent spleen samples, 0.4% (2/458) and 2.4% (11/458) were positive for Ehrlichia and Anaplasma spp., respectively. Of 458 samples, 2.0% (9/458) and 1.1% (5/458) were positive for Anaplasma sp. and Ehrlichia sp., respectively, using conventional 16S rRNA PCR assays. Maximum Likelihood phylogenetic analyse based on a small region of 16S rRNA genes positioned the Anaplasma genotypes in rodents near Anaplasma phagocytophilum or Anaplasma marginale and Anaplasma odocoilei isolates. Ehrlichia genotypes were closely related to E. canis. There was a low occurrence of Anaplasma and Ehrlichia in wild and synanthropic rodents in Brazil, suggesting the circulation of new genotypes of these agents in rodents in the studied areas. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 2018-12-11T17:32:12Z 2018-12-11T17:32:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ttbdis.2017.04.011 Ticks and Tick-borne Diseases, v. 8, n. 4, p. 646-656, 2017. 1877-9603 1877-959X http://hdl.handle.net/11449/178811 10.1016/j.ttbdis.2017.04.011 2-s2.0-85018159800 2-s2.0-85018159800.pdf 9139899895580513 |
url |
http://dx.doi.org/10.1016/j.ttbdis.2017.04.011 http://hdl.handle.net/11449/178811 |
identifier_str_mv |
Ticks and Tick-borne Diseases, v. 8, n. 4, p. 646-656, 2017. 1877-9603 1877-959X 10.1016/j.ttbdis.2017.04.011 2-s2.0-85018159800 2-s2.0-85018159800.pdf 9139899895580513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ticks and Tick-borne Diseases 1,421 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
646-656 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128568408932352 |