Análise proteômica sugere adaptação do músculo gastrocnêmio de camundongos com diabetes tipo II induzida por estreptozotocina e dieta hiperlipídica

• Autor(a) principal: Rodrigues, Chelsea Maria Vilas Boas Feitosa
• Data de Publicação: 2022
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/217652
• topic: Skeletal muscle; Proteomics; Diabetes; Músculo esquelético; Proteômica

Type II diabetes mellitus is the most common, its prevalence has reached increasing global levels that demand a high economic expense and increase the propensity to vascular, cardiac and muscular diseases. In this scenario, proteomics has the potential to provide information about metabolic changes in carriers by identifying pathways and possible markers involved. Thus, the objective of this work was to analyze the proteomic profile of the gastrocnemius muscle of mice induced with type 2 diabetes to identify alterations and the influence of the high-fat diet. Eighteen adult male mice were divided into 3 experimental groups (n=6/group) namely: non-diabetic control (CND), streptozotocin-induced diabetic (CDD) and streptozotocin-induced diabetic and high-fat diet (DHL), they received 8 weeks high-fat diet (DHL group) or normocaloric diet (CND and CDD), after this period the DHL and CDD groups received successive intraperitoneal injections of streptozotocin (STZ) from 40 to 50mg/kg for 3-5 days. After the induction of diabetes was verified, the animals were kept for another 21 days under these conditions, and then at the end they were euthanized and the muscle was collected. The gastrocnemius muscle was submitted to proteomic analysis. The following comparisons were analyzed: CDD X CND; DHL X CND and DHL X CDD. The functional classification analysis showed three categories with the greatest changes: muscle contraction, energy metabolism and carbohydrate metabolism. The results of the quantitative analysis indicated that the high-fat diet group showed positive regulation of several proteins, indicating a possible adaptive effect triggered by the high-fat diet. The CDD X CND comparison showed predominant negative regulation in the three categories. These results may indicate that muscle function is affected by diabetes and high-fat diet by activating adaptive pathways in the muscle, in addition to identifying the most affected functions. These data have the potential to expand the knowledge we have about diabetes and the mechanisms involved in the pathology.