Correlation between koilocytes and human papillomavirus detection by PCR in oral and oropharynx squamous cell carcinoma biopsies

Detalhes bibliográficos
Autor(a) principal: Miyahara, Glauco Issamu [UNESP]
Data de Publicação: 2011
Outros Autores: Simonato, Luciana Estevam [UNESP], Mattar, Neivio José [UNESP], Camilo Jr, Deolino João [UNESP], Biasoli, Eder Ricardo [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200008
http://hdl.handle.net/11449/15625
Resumo: The purpose of this study was to compare the histopathological analysis with polymerase chain reaction (PCR) methods to predict the presence of human papillomavirus (HPV) infection in oral squamous cell carcinoma biopsies. Eighty-three paraffin-embedded tissue specimens from patients with oropharynx and mouth floor squamous cell carcinoma were submitted to histopathological analysis under light microscopy, specifically for the determination of the presence of koilocytes. Subsequently, DNA was purified from the same paraffin-embedded specimens and submitted to PCR. Fisher's exact test showed no statistically significant correlation between the two methods. The results suggest that the presence of koilocytes is unreliable for the detection of HPV presence in oral and oropharynx squamous cell carcinoma.
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spelling Correlation between koilocytes and human papillomavirus detection by PCR in oral and oropharynx squamous cell carcinoma biopsieshuman papillomavirussquamous cell carcinomamicroscopypolymerase chain reactionThe purpose of this study was to compare the histopathological analysis with polymerase chain reaction (PCR) methods to predict the presence of human papillomavirus (HPV) infection in oral squamous cell carcinoma biopsies. Eighty-three paraffin-embedded tissue specimens from patients with oropharynx and mouth floor squamous cell carcinoma were submitted to histopathological analysis under light microscopy, specifically for the determination of the presence of koilocytes. Subsequently, DNA was purified from the same paraffin-embedded specimens and submitted to PCR. Fisher's exact test showed no statistically significant correlation between the two methods. The results suggest that the presence of koilocytes is unreliable for the detection of HPV presence in oral and oropharynx squamous cell carcinoma.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)PROINTER (UNESP)Universidade Estadual Paulista Faculdade de Odontologia de Araçatuba Centro de Oncologia BucalUniversidade Estadual Paulista Faculdade de Odontologia de Araçatuba Centro de Oncologia BucalInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Estadual Paulista (Unesp)Miyahara, Glauco Issamu [UNESP]Simonato, Luciana Estevam [UNESP]Mattar, Neivio José [UNESP]Camilo Jr, Deolino João [UNESP]Biasoli, Eder Ricardo [UNESP]2013-09-30T19:34:45Z2014-05-20T13:44:34Z2013-09-30T19:34:45Z2014-05-20T13:44:34Z2011-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article166-169application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200008Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 2, p. 166-169, 2011.0074-0276http://hdl.handle.net/11449/1562510.1590/S0074-02762011000200008S0074-02762011000200008WOS:000289961100008S0074-02762011000200008.pdf38468911670832110000-0002-5326-2026SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2024-04-11T20:16:33Zoai:repositorio.unesp.br:11449/15625Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:44:34.172028Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Correlation between koilocytes and human papillomavirus detection by PCR in oral and oropharynx squamous cell carcinoma biopsies
title Correlation between koilocytes and human papillomavirus detection by PCR in oral and oropharynx squamous cell carcinoma biopsies
spellingShingle Correlation between koilocytes and human papillomavirus detection by PCR in oral and oropharynx squamous cell carcinoma biopsies
Miyahara, Glauco Issamu [UNESP]
human papillomavirus
squamous cell carcinoma
microscopy
polymerase chain reaction
title_short Correlation between koilocytes and human papillomavirus detection by PCR in oral and oropharynx squamous cell carcinoma biopsies
title_full Correlation between koilocytes and human papillomavirus detection by PCR in oral and oropharynx squamous cell carcinoma biopsies
title_fullStr Correlation between koilocytes and human papillomavirus detection by PCR in oral and oropharynx squamous cell carcinoma biopsies
title_full_unstemmed Correlation between koilocytes and human papillomavirus detection by PCR in oral and oropharynx squamous cell carcinoma biopsies
title_sort Correlation between koilocytes and human papillomavirus detection by PCR in oral and oropharynx squamous cell carcinoma biopsies
author Miyahara, Glauco Issamu [UNESP]
author_facet Miyahara, Glauco Issamu [UNESP]
Simonato, Luciana Estevam [UNESP]
Mattar, Neivio José [UNESP]
Camilo Jr, Deolino João [UNESP]
Biasoli, Eder Ricardo [UNESP]
author_role author
author2 Simonato, Luciana Estevam [UNESP]
Mattar, Neivio José [UNESP]
Camilo Jr, Deolino João [UNESP]
Biasoli, Eder Ricardo [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Miyahara, Glauco Issamu [UNESP]
Simonato, Luciana Estevam [UNESP]
Mattar, Neivio José [UNESP]
Camilo Jr, Deolino João [UNESP]
Biasoli, Eder Ricardo [UNESP]
dc.subject.por.fl_str_mv human papillomavirus
squamous cell carcinoma
microscopy
polymerase chain reaction
topic human papillomavirus
squamous cell carcinoma
microscopy
polymerase chain reaction
description The purpose of this study was to compare the histopathological analysis with polymerase chain reaction (PCR) methods to predict the presence of human papillomavirus (HPV) infection in oral squamous cell carcinoma biopsies. Eighty-three paraffin-embedded tissue specimens from patients with oropharynx and mouth floor squamous cell carcinoma were submitted to histopathological analysis under light microscopy, specifically for the determination of the presence of koilocytes. Subsequently, DNA was purified from the same paraffin-embedded specimens and submitted to PCR. Fisher's exact test showed no statistically significant correlation between the two methods. The results suggest that the presence of koilocytes is unreliable for the detection of HPV presence in oral and oropharynx squamous cell carcinoma.
publishDate 2011
dc.date.none.fl_str_mv 2011-03-01
2013-09-30T19:34:45Z
2013-09-30T19:34:45Z
2014-05-20T13:44:34Z
2014-05-20T13:44:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200008
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 2, p. 166-169, 2011.
0074-0276
http://hdl.handle.net/11449/15625
10.1590/S0074-02762011000200008
S0074-02762011000200008
WOS:000289961100008
S0074-02762011000200008.pdf
3846891167083211
0000-0002-5326-2026
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200008
http://hdl.handle.net/11449/15625
identifier_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 2, p. 166-169, 2011.
0074-0276
10.1590/S0074-02762011000200008
S0074-02762011000200008
WOS:000289961100008
S0074-02762011000200008.pdf
3846891167083211
0000-0002-5326-2026
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
2.833
1,172
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 166-169
application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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