Sulfate removal rate and metal recovery as settling precipitates in bioreactors: Influence of electron donors
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jhazmat.2020.123622 http://hdl.handle.net/11449/209732 |
Resumo: | Four down-flow structured bed bioreactors were operated targeting biological sulfate-reduction and metal recovery. Three different electron donors were tested: glycerol (R1), lactate (R2), sucrose (R3), and a blend of the previous three (R4) with an increasing copper influent load (5, 15, and 30 mg Cu2+.L-1). Copper inhibited sulfate-reduction in R1 (15 mg Cu2+.L-1) and R3 (5 mg Cu2+.L-1), but the fermentative activity was not affected. R2 and R4 were not inhibited by the copper influent concentration. R2 provided the highest sulfate reduction rate (1767.3 +/- 240.1 mg SO42-.L.day(-1)). Nonetheless, the accumulation of settling precipitates was 22 % higher in R4 than in R2, indicating the former yielded the highest metal recovery as settling precipitates (24.8 g FSS.L-1, 25 % Fe2+, 5% Cu2+). 16S rRNA sequencing showed highest diversity of sulfate-reducing bacteria in R2. A predominance of sulfate-reducing and fermentative bacteria with more similarity was observed between microbial populations in R1 and R4, despite the difference in toxicity thresholds. Hence, the electron donor influenced not only the biological sulfate reduction, but also metal toxicity thresholds and metal recovery as settling precipitates. |
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Sulfate removal rate and metal recovery as settling precipitates in bioreactors: Influence of electron donorsAcid mine drainageSulfidogenenis16 rRNA sequencingCopper toxicityMetal precipitationDown-flow structured-bed reactorsFour down-flow structured bed bioreactors were operated targeting biological sulfate-reduction and metal recovery. Three different electron donors were tested: glycerol (R1), lactate (R2), sucrose (R3), and a blend of the previous three (R4) with an increasing copper influent load (5, 15, and 30 mg Cu2+.L-1). Copper inhibited sulfate-reduction in R1 (15 mg Cu2+.L-1) and R3 (5 mg Cu2+.L-1), but the fermentative activity was not affected. R2 and R4 were not inhibited by the copper influent concentration. R2 provided the highest sulfate reduction rate (1767.3 +/- 240.1 mg SO42-.L.day(-1)). Nonetheless, the accumulation of settling precipitates was 22 % higher in R4 than in R2, indicating the former yielded the highest metal recovery as settling precipitates (24.8 g FSS.L-1, 25 % Fe2+, 5% Cu2+). 16S rRNA sequencing showed highest diversity of sulfate-reducing bacteria in R2. A predominance of sulfate-reducing and fermentative bacteria with more similarity was observed between microbial populations in R1 and R4, despite the difference in toxicity thresholds. Hence, the electron donor influenced not only the biological sulfate reduction, but also metal toxicity thresholds and metal recovery as settling precipitates.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Inst Chem, Dept Biochem & Organ Chem, R Francisco Degni 55, BR-14800060 Araraquara, SP, BrazilUniv Sao Paulo, Sao Carlos Sch Engn EESC, Dept Hydraul & Sanitat, Biol Proc Lab, Engn Ambiental Bloco 4-F,1100 Joao Dagnone Av, BR-13563120 Sao Carlos, SP, BrazilUniv Fed Sao Carlos, Dept Biol, Lab Environm Microbiol, Rodovia Joao Leme Santos Km 110, BR-18052780 Sorocaba, SP, BrazilSao Paulo State Univ, Inst Chem, Dept Biochem & Organ Chem, R Francisco Degni 55, BR-14800060 Araraquara, SP, BrazilFAPESP: FAPESP 2016/13603-3FAPESP: FAPESP2018/00213-8Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Universidade Federal de São Carlos (UFSCar)Costa, Rachel Biancalana [UNESP]Gouvea Godoi, Leandro AugustoMaluf Braga, Adriana FerreiraDelforno, Tiago PalladinoBevilaqua, Denise [UNESP]2021-06-25T12:27:25Z2021-06-25T12:27:25Z2021-02-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11http://dx.doi.org/10.1016/j.jhazmat.2020.123622Journal Of Hazardous Materials. Amsterdam: Elsevier, v. 403, 11 p., 2021.0304-3894http://hdl.handle.net/11449/20973210.1016/j.jhazmat.2020.123622WOS:000595083800004Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Hazardous Materialsinfo:eu-repo/semantics/openAccess2021-10-23T19:49:59Zoai:repositorio.unesp.br:11449/209732Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:01:33.467057Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Sulfate removal rate and metal recovery as settling precipitates in bioreactors: Influence of electron donors |
title |
Sulfate removal rate and metal recovery as settling precipitates in bioreactors: Influence of electron donors |
spellingShingle |
Sulfate removal rate and metal recovery as settling precipitates in bioreactors: Influence of electron donors Costa, Rachel Biancalana [UNESP] Acid mine drainage Sulfidogenenis 16 rRNA sequencing Copper toxicity Metal precipitation Down-flow structured-bed reactors |
title_short |
Sulfate removal rate and metal recovery as settling precipitates in bioreactors: Influence of electron donors |
title_full |
Sulfate removal rate and metal recovery as settling precipitates in bioreactors: Influence of electron donors |
title_fullStr |
Sulfate removal rate and metal recovery as settling precipitates in bioreactors: Influence of electron donors |
title_full_unstemmed |
Sulfate removal rate and metal recovery as settling precipitates in bioreactors: Influence of electron donors |
title_sort |
Sulfate removal rate and metal recovery as settling precipitates in bioreactors: Influence of electron donors |
author |
Costa, Rachel Biancalana [UNESP] |
author_facet |
Costa, Rachel Biancalana [UNESP] Gouvea Godoi, Leandro Augusto Maluf Braga, Adriana Ferreira Delforno, Tiago Palladino Bevilaqua, Denise [UNESP] |
author_role |
author |
author2 |
Gouvea Godoi, Leandro Augusto Maluf Braga, Adriana Ferreira Delforno, Tiago Palladino Bevilaqua, Denise [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Costa, Rachel Biancalana [UNESP] Gouvea Godoi, Leandro Augusto Maluf Braga, Adriana Ferreira Delforno, Tiago Palladino Bevilaqua, Denise [UNESP] |
dc.subject.por.fl_str_mv |
Acid mine drainage Sulfidogenenis 16 rRNA sequencing Copper toxicity Metal precipitation Down-flow structured-bed reactors |
topic |
Acid mine drainage Sulfidogenenis 16 rRNA sequencing Copper toxicity Metal precipitation Down-flow structured-bed reactors |
description |
Four down-flow structured bed bioreactors were operated targeting biological sulfate-reduction and metal recovery. Three different electron donors were tested: glycerol (R1), lactate (R2), sucrose (R3), and a blend of the previous three (R4) with an increasing copper influent load (5, 15, and 30 mg Cu2+.L-1). Copper inhibited sulfate-reduction in R1 (15 mg Cu2+.L-1) and R3 (5 mg Cu2+.L-1), but the fermentative activity was not affected. R2 and R4 were not inhibited by the copper influent concentration. R2 provided the highest sulfate reduction rate (1767.3 +/- 240.1 mg SO42-.L.day(-1)). Nonetheless, the accumulation of settling precipitates was 22 % higher in R4 than in R2, indicating the former yielded the highest metal recovery as settling precipitates (24.8 g FSS.L-1, 25 % Fe2+, 5% Cu2+). 16S rRNA sequencing showed highest diversity of sulfate-reducing bacteria in R2. A predominance of sulfate-reducing and fermentative bacteria with more similarity was observed between microbial populations in R1 and R4, despite the difference in toxicity thresholds. Hence, the electron donor influenced not only the biological sulfate reduction, but also metal toxicity thresholds and metal recovery as settling precipitates. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T12:27:25Z 2021-06-25T12:27:25Z 2021-02-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jhazmat.2020.123622 Journal Of Hazardous Materials. Amsterdam: Elsevier, v. 403, 11 p., 2021. 0304-3894 http://hdl.handle.net/11449/209732 10.1016/j.jhazmat.2020.123622 WOS:000595083800004 |
url |
http://dx.doi.org/10.1016/j.jhazmat.2020.123622 http://hdl.handle.net/11449/209732 |
identifier_str_mv |
Journal Of Hazardous Materials. Amsterdam: Elsevier, v. 403, 11 p., 2021. 0304-3894 10.1016/j.jhazmat.2020.123622 WOS:000595083800004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Hazardous Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128740725620736 |