Folic acid-modified curcumin-loaded liposomes for breast cancer therapy

Detalhes bibliográficos
Autor(a) principal: Luiz, Marcela Tavares
Data de Publicação: 2022
Outros Autores: Dutra, Jessyca Aparecida Paes [UNESP], Ribeiro, Taís de Cássia [UNESP], Carvalho, Gabriela Corrêa [UNESP], Sábio, Rafael Miguel [UNESP], Marchetti, Juliana Maldonado, Chorilli, Marlus [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.colsurfa.2022.128935
http://hdl.handle.net/11449/234360
Resumo: Breast cancer is the commonest cancer among women, with more than 2 million cases per year and a lethality rate of 17.7%. The treatment of breast cancer still being a challenge due to the high heterogeneity of tumor cells. In this scenario, the development of targeted nanosystems for drug delivery has been investigated. Thus, the aim of this study was the development and characterization of folic acid-modified curcumin-loaded liposomes (LIP-CCM-FA) for the treatment of breast cancer. For this purpose, LIP-CCM-FA and unmodified liposomes (LIP-CCM) were developed and characterized in vitro using MCF-7 cells culture in two-dimensional (2D) and three-dimensional (3D) models. LIP-CCM and LIP-CCM-FA showed low particles size (~ 138 nm), narrow polydispersity indexes (~ 0.140), negative zeta potential (~ −13 mV), and high drug encapsulation efficiency (> 73%). Furthermore, LIP-CCM-FA showed a significantly greater cytotoxicity effect when compared with free curcumin and LIP-CCM using 2D and 3D cell culture models. In addition, in vitro cellular uptake assays using 2D and 3D models indicated higher cellular internalization and spheroid penetration of LIP-CCM-FA when compared with free curcumin and LIP-CCM, suggesting the potential of folic acid modification for improving drug internalization in breast cancer cells through its recognition by folate receptors overexpressed. In summary, this novel nanosystem demonstrated a greater in vitro antitumoral effect due to its ability to permeate tissues and be specifically recognized by folate receptors.
id UNSP_0dab279700713e5ad31075ee02095bf0
oai_identifier_str oai:repositorio.unesp.br:11449/234360
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Folic acid-modified curcumin-loaded liposomes for breast cancer therapyActive targetingDrug delivery systemsFolateLiposomalNanoparticlesNanotechnologyBreast cancer is the commonest cancer among women, with more than 2 million cases per year and a lethality rate of 17.7%. The treatment of breast cancer still being a challenge due to the high heterogeneity of tumor cells. In this scenario, the development of targeted nanosystems for drug delivery has been investigated. Thus, the aim of this study was the development and characterization of folic acid-modified curcumin-loaded liposomes (LIP-CCM-FA) for the treatment of breast cancer. For this purpose, LIP-CCM-FA and unmodified liposomes (LIP-CCM) were developed and characterized in vitro using MCF-7 cells culture in two-dimensional (2D) and three-dimensional (3D) models. LIP-CCM and LIP-CCM-FA showed low particles size (~ 138 nm), narrow polydispersity indexes (~ 0.140), negative zeta potential (~ −13 mV), and high drug encapsulation efficiency (> 73%). Furthermore, LIP-CCM-FA showed a significantly greater cytotoxicity effect when compared with free curcumin and LIP-CCM using 2D and 3D cell culture models. In addition, in vitro cellular uptake assays using 2D and 3D models indicated higher cellular internalization and spheroid penetration of LIP-CCM-FA when compared with free curcumin and LIP-CCM, suggesting the potential of folic acid modification for improving drug internalization in breast cancer cells through its recognition by folate receptors overexpressed. In summary, this novel nanosystem demonstrated a greater in vitro antitumoral effect due to its ability to permeate tissues and be specifically recognized by folate receptors.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)School of Pharmaceutical Science of Ribeirao Preto University of Sao Paulo (USP)School of Pharmaceutical Science of Sao Paulo State University (UNESP), Sao PauloSchool of Pharmaceutical Science of Sao Paulo State University (UNESP), Sao PauloCAPES: 001Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Luiz, Marcela TavaresDutra, Jessyca Aparecida Paes [UNESP]Ribeiro, Taís de Cássia [UNESP]Carvalho, Gabriela Corrêa [UNESP]Sábio, Rafael Miguel [UNESP]Marchetti, Juliana MaldonadoChorilli, Marlus [UNESP]2022-05-01T16:48:26Z2022-05-01T16:48:26Z2022-07-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.colsurfa.2022.128935Colloids and Surfaces A: Physicochemical and Engineering Aspects, v. 645.1873-43590927-7757http://hdl.handle.net/11449/23436010.1016/j.colsurfa.2022.1289352-s2.0-85127662400Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengColloids and Surfaces A: Physicochemical and Engineering Aspectsinfo:eu-repo/semantics/openAccess2024-06-24T13:46:12Zoai:repositorio.unesp.br:11449/234360Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:31:03.333926Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Folic acid-modified curcumin-loaded liposomes for breast cancer therapy
title Folic acid-modified curcumin-loaded liposomes for breast cancer therapy
spellingShingle Folic acid-modified curcumin-loaded liposomes for breast cancer therapy
Luiz, Marcela Tavares
Active targeting
Drug delivery systems
Folate
Liposomal
Nanoparticles
Nanotechnology
title_short Folic acid-modified curcumin-loaded liposomes for breast cancer therapy
title_full Folic acid-modified curcumin-loaded liposomes for breast cancer therapy
title_fullStr Folic acid-modified curcumin-loaded liposomes for breast cancer therapy
title_full_unstemmed Folic acid-modified curcumin-loaded liposomes for breast cancer therapy
title_sort Folic acid-modified curcumin-loaded liposomes for breast cancer therapy
author Luiz, Marcela Tavares
author_facet Luiz, Marcela Tavares
Dutra, Jessyca Aparecida Paes [UNESP]
Ribeiro, Taís de Cássia [UNESP]
Carvalho, Gabriela Corrêa [UNESP]
Sábio, Rafael Miguel [UNESP]
Marchetti, Juliana Maldonado
Chorilli, Marlus [UNESP]
author_role author
author2 Dutra, Jessyca Aparecida Paes [UNESP]
Ribeiro, Taís de Cássia [UNESP]
Carvalho, Gabriela Corrêa [UNESP]
Sábio, Rafael Miguel [UNESP]
Marchetti, Juliana Maldonado
Chorilli, Marlus [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Luiz, Marcela Tavares
Dutra, Jessyca Aparecida Paes [UNESP]
Ribeiro, Taís de Cássia [UNESP]
Carvalho, Gabriela Corrêa [UNESP]
Sábio, Rafael Miguel [UNESP]
Marchetti, Juliana Maldonado
Chorilli, Marlus [UNESP]
dc.subject.por.fl_str_mv Active targeting
Drug delivery systems
Folate
Liposomal
Nanoparticles
Nanotechnology
topic Active targeting
Drug delivery systems
Folate
Liposomal
Nanoparticles
Nanotechnology
description Breast cancer is the commonest cancer among women, with more than 2 million cases per year and a lethality rate of 17.7%. The treatment of breast cancer still being a challenge due to the high heterogeneity of tumor cells. In this scenario, the development of targeted nanosystems for drug delivery has been investigated. Thus, the aim of this study was the development and characterization of folic acid-modified curcumin-loaded liposomes (LIP-CCM-FA) for the treatment of breast cancer. For this purpose, LIP-CCM-FA and unmodified liposomes (LIP-CCM) were developed and characterized in vitro using MCF-7 cells culture in two-dimensional (2D) and three-dimensional (3D) models. LIP-CCM and LIP-CCM-FA showed low particles size (~ 138 nm), narrow polydispersity indexes (~ 0.140), negative zeta potential (~ −13 mV), and high drug encapsulation efficiency (> 73%). Furthermore, LIP-CCM-FA showed a significantly greater cytotoxicity effect when compared with free curcumin and LIP-CCM using 2D and 3D cell culture models. In addition, in vitro cellular uptake assays using 2D and 3D models indicated higher cellular internalization and spheroid penetration of LIP-CCM-FA when compared with free curcumin and LIP-CCM, suggesting the potential of folic acid modification for improving drug internalization in breast cancer cells through its recognition by folate receptors overexpressed. In summary, this novel nanosystem demonstrated a greater in vitro antitumoral effect due to its ability to permeate tissues and be specifically recognized by folate receptors.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-01T16:48:26Z
2022-05-01T16:48:26Z
2022-07-20
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.colsurfa.2022.128935
Colloids and Surfaces A: Physicochemical and Engineering Aspects, v. 645.
1873-4359
0927-7757
http://hdl.handle.net/11449/234360
10.1016/j.colsurfa.2022.128935
2-s2.0-85127662400
url http://dx.doi.org/10.1016/j.colsurfa.2022.128935
http://hdl.handle.net/11449/234360
identifier_str_mv Colloids and Surfaces A: Physicochemical and Engineering Aspects, v. 645.
1873-4359
0927-7757
10.1016/j.colsurfa.2022.128935
2-s2.0-85127662400
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Colloids and Surfaces A: Physicochemical and Engineering Aspects
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129329426595840