Folic acid-modified curcumin-loaded liposomes for breast cancer therapy
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.colsurfa.2022.128935 http://hdl.handle.net/11449/234360 |
Resumo: | Breast cancer is the commonest cancer among women, with more than 2 million cases per year and a lethality rate of 17.7%. The treatment of breast cancer still being a challenge due to the high heterogeneity of tumor cells. In this scenario, the development of targeted nanosystems for drug delivery has been investigated. Thus, the aim of this study was the development and characterization of folic acid-modified curcumin-loaded liposomes (LIP-CCM-FA) for the treatment of breast cancer. For this purpose, LIP-CCM-FA and unmodified liposomes (LIP-CCM) were developed and characterized in vitro using MCF-7 cells culture in two-dimensional (2D) and three-dimensional (3D) models. LIP-CCM and LIP-CCM-FA showed low particles size (~ 138 nm), narrow polydispersity indexes (~ 0.140), negative zeta potential (~ −13 mV), and high drug encapsulation efficiency (> 73%). Furthermore, LIP-CCM-FA showed a significantly greater cytotoxicity effect when compared with free curcumin and LIP-CCM using 2D and 3D cell culture models. In addition, in vitro cellular uptake assays using 2D and 3D models indicated higher cellular internalization and spheroid penetration of LIP-CCM-FA when compared with free curcumin and LIP-CCM, suggesting the potential of folic acid modification for improving drug internalization in breast cancer cells through its recognition by folate receptors overexpressed. In summary, this novel nanosystem demonstrated a greater in vitro antitumoral effect due to its ability to permeate tissues and be specifically recognized by folate receptors. |
id |
UNSP_0dab279700713e5ad31075ee02095bf0 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/234360 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Folic acid-modified curcumin-loaded liposomes for breast cancer therapyActive targetingDrug delivery systemsFolateLiposomalNanoparticlesNanotechnologyBreast cancer is the commonest cancer among women, with more than 2 million cases per year and a lethality rate of 17.7%. The treatment of breast cancer still being a challenge due to the high heterogeneity of tumor cells. In this scenario, the development of targeted nanosystems for drug delivery has been investigated. Thus, the aim of this study was the development and characterization of folic acid-modified curcumin-loaded liposomes (LIP-CCM-FA) for the treatment of breast cancer. For this purpose, LIP-CCM-FA and unmodified liposomes (LIP-CCM) were developed and characterized in vitro using MCF-7 cells culture in two-dimensional (2D) and three-dimensional (3D) models. LIP-CCM and LIP-CCM-FA showed low particles size (~ 138 nm), narrow polydispersity indexes (~ 0.140), negative zeta potential (~ −13 mV), and high drug encapsulation efficiency (> 73%). Furthermore, LIP-CCM-FA showed a significantly greater cytotoxicity effect when compared with free curcumin and LIP-CCM using 2D and 3D cell culture models. In addition, in vitro cellular uptake assays using 2D and 3D models indicated higher cellular internalization and spheroid penetration of LIP-CCM-FA when compared with free curcumin and LIP-CCM, suggesting the potential of folic acid modification for improving drug internalization in breast cancer cells through its recognition by folate receptors overexpressed. In summary, this novel nanosystem demonstrated a greater in vitro antitumoral effect due to its ability to permeate tissues and be specifically recognized by folate receptors.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)School of Pharmaceutical Science of Ribeirao Preto University of Sao Paulo (USP)School of Pharmaceutical Science of Sao Paulo State University (UNESP), Sao PauloSchool of Pharmaceutical Science of Sao Paulo State University (UNESP), Sao PauloCAPES: 001Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Luiz, Marcela TavaresDutra, Jessyca Aparecida Paes [UNESP]Ribeiro, Taís de Cássia [UNESP]Carvalho, Gabriela Corrêa [UNESP]Sábio, Rafael Miguel [UNESP]Marchetti, Juliana MaldonadoChorilli, Marlus [UNESP]2022-05-01T16:48:26Z2022-05-01T16:48:26Z2022-07-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.colsurfa.2022.128935Colloids and Surfaces A: Physicochemical and Engineering Aspects, v. 645.1873-43590927-7757http://hdl.handle.net/11449/23436010.1016/j.colsurfa.2022.1289352-s2.0-85127662400Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengColloids and Surfaces A: Physicochemical and Engineering Aspectsinfo:eu-repo/semantics/openAccess2024-06-24T13:46:12Zoai:repositorio.unesp.br:11449/234360Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:31:03.333926Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Folic acid-modified curcumin-loaded liposomes for breast cancer therapy |
title |
Folic acid-modified curcumin-loaded liposomes for breast cancer therapy |
spellingShingle |
Folic acid-modified curcumin-loaded liposomes for breast cancer therapy Luiz, Marcela Tavares Active targeting Drug delivery systems Folate Liposomal Nanoparticles Nanotechnology |
title_short |
Folic acid-modified curcumin-loaded liposomes for breast cancer therapy |
title_full |
Folic acid-modified curcumin-loaded liposomes for breast cancer therapy |
title_fullStr |
Folic acid-modified curcumin-loaded liposomes for breast cancer therapy |
title_full_unstemmed |
Folic acid-modified curcumin-loaded liposomes for breast cancer therapy |
title_sort |
Folic acid-modified curcumin-loaded liposomes for breast cancer therapy |
author |
Luiz, Marcela Tavares |
author_facet |
Luiz, Marcela Tavares Dutra, Jessyca Aparecida Paes [UNESP] Ribeiro, Taís de Cássia [UNESP] Carvalho, Gabriela Corrêa [UNESP] Sábio, Rafael Miguel [UNESP] Marchetti, Juliana Maldonado Chorilli, Marlus [UNESP] |
author_role |
author |
author2 |
Dutra, Jessyca Aparecida Paes [UNESP] Ribeiro, Taís de Cássia [UNESP] Carvalho, Gabriela Corrêa [UNESP] Sábio, Rafael Miguel [UNESP] Marchetti, Juliana Maldonado Chorilli, Marlus [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Luiz, Marcela Tavares Dutra, Jessyca Aparecida Paes [UNESP] Ribeiro, Taís de Cássia [UNESP] Carvalho, Gabriela Corrêa [UNESP] Sábio, Rafael Miguel [UNESP] Marchetti, Juliana Maldonado Chorilli, Marlus [UNESP] |
dc.subject.por.fl_str_mv |
Active targeting Drug delivery systems Folate Liposomal Nanoparticles Nanotechnology |
topic |
Active targeting Drug delivery systems Folate Liposomal Nanoparticles Nanotechnology |
description |
Breast cancer is the commonest cancer among women, with more than 2 million cases per year and a lethality rate of 17.7%. The treatment of breast cancer still being a challenge due to the high heterogeneity of tumor cells. In this scenario, the development of targeted nanosystems for drug delivery has been investigated. Thus, the aim of this study was the development and characterization of folic acid-modified curcumin-loaded liposomes (LIP-CCM-FA) for the treatment of breast cancer. For this purpose, LIP-CCM-FA and unmodified liposomes (LIP-CCM) were developed and characterized in vitro using MCF-7 cells culture in two-dimensional (2D) and three-dimensional (3D) models. LIP-CCM and LIP-CCM-FA showed low particles size (~ 138 nm), narrow polydispersity indexes (~ 0.140), negative zeta potential (~ −13 mV), and high drug encapsulation efficiency (> 73%). Furthermore, LIP-CCM-FA showed a significantly greater cytotoxicity effect when compared with free curcumin and LIP-CCM using 2D and 3D cell culture models. In addition, in vitro cellular uptake assays using 2D and 3D models indicated higher cellular internalization and spheroid penetration of LIP-CCM-FA when compared with free curcumin and LIP-CCM, suggesting the potential of folic acid modification for improving drug internalization in breast cancer cells through its recognition by folate receptors overexpressed. In summary, this novel nanosystem demonstrated a greater in vitro antitumoral effect due to its ability to permeate tissues and be specifically recognized by folate receptors. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01T16:48:26Z 2022-05-01T16:48:26Z 2022-07-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.colsurfa.2022.128935 Colloids and Surfaces A: Physicochemical and Engineering Aspects, v. 645. 1873-4359 0927-7757 http://hdl.handle.net/11449/234360 10.1016/j.colsurfa.2022.128935 2-s2.0-85127662400 |
url |
http://dx.doi.org/10.1016/j.colsurfa.2022.128935 http://hdl.handle.net/11449/234360 |
identifier_str_mv |
Colloids and Surfaces A: Physicochemical and Engineering Aspects, v. 645. 1873-4359 0927-7757 10.1016/j.colsurfa.2022.128935 2-s2.0-85127662400 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Colloids and Surfaces A: Physicochemical and Engineering Aspects |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129329426595840 |