Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production

Detalhes bibliográficos
Autor(a) principal: Dias de Oliveira, Luciane [UNESP]
Data de Publicação: 2022
Outros Autores: de Oliveira, Felipe Eduardo [UNESP], Hatje, Bárbara Araujo [UNESP], Valera, Marcia Carneiro [UNESP], Carvalho, Cláudio Antonio Talge [UNESP], Hasna, Amjad Abu [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0103-6440202205195
http://hdl.handle.net/11449/248002
Resumo: The aim of this study was to evaluate in vitro the effect of calcium hydroxide [Ca(OH)2], 2% chlorhexidine gel (CHX) on macrophages (RAW 264.7) to produce pro-inflammatory cytokines and nitric oxide after pretreatment with lipoteichoic acid (LTA) of Enterococcus faecalis. Forty-eight human single- rooted teeth were instrumented with R25.08 (RECIPROC) and sterilized by gamma irradiation. LTA was inoculated in the root canal of each specimen for 96 hours. Specimens were instrumented with 40.06 and 50.05 (RECIPROC) and medicated with: I) Pyrogen-free saline solution (SS); II) 2% CHX gel; III) Ca(OH)2 + SS; or IV) Ca(OH)2 + CHX for 14 days. Three samples (S) were performed of the root canal of each specimen at: S1) immediately after instrumentation; S2) after Ethylenediaminetetraacetic acid (EDTA); S3) after intracanal medication removal. Subsequent quantification of cytokines (IL-1 β, TNF-α, MIP-1α, IP-10, G-CSF and IL-6) by immunosorbent assay (ELISA) and nitric oxide by the Griess method was carried-out. Data were submitted to a normality test and then analyzed with one-way ANOVA and Tukey test with a significance level of 5% using GraphPad Prism 6. Ca(OH)2 + SS and Ca(OH)2 + CHX presented lower levels of TNF-α, TNF-α, IL-6, G-CSF and nitric oxide. Ca(OH)2 + SS was the most effective in reducing MIP-1α. CHX was effective in reducing IL-6 and G-CSF. Therefore, the combined intracanal medication of calcium hydroxide and chlorhexidine is effective in reducing the cytokines TNF-α, IL-1β, IL-6, G-CSF and nitric oxide.
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spelling Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines productioncalcium hydroxidechlorhexidinecytokineslipoteichoic acidmacrophagesThe aim of this study was to evaluate in vitro the effect of calcium hydroxide [Ca(OH)2], 2% chlorhexidine gel (CHX) on macrophages (RAW 264.7) to produce pro-inflammatory cytokines and nitric oxide after pretreatment with lipoteichoic acid (LTA) of Enterococcus faecalis. Forty-eight human single- rooted teeth were instrumented with R25.08 (RECIPROC) and sterilized by gamma irradiation. LTA was inoculated in the root canal of each specimen for 96 hours. Specimens were instrumented with 40.06 and 50.05 (RECIPROC) and medicated with: I) Pyrogen-free saline solution (SS); II) 2% CHX gel; III) Ca(OH)2 + SS; or IV) Ca(OH)2 + CHX for 14 days. Three samples (S) were performed of the root canal of each specimen at: S1) immediately after instrumentation; S2) after Ethylenediaminetetraacetic acid (EDTA); S3) after intracanal medication removal. Subsequent quantification of cytokines (IL-1 β, TNF-α, MIP-1α, IP-10, G-CSF and IL-6) by immunosorbent assay (ELISA) and nitric oxide by the Griess method was carried-out. Data were submitted to a normality test and then analyzed with one-way ANOVA and Tukey test with a significance level of 5% using GraphPad Prism 6. Ca(OH)2 + SS and Ca(OH)2 + CHX presented lower levels of TNF-α, TNF-α, IL-6, G-CSF and nitric oxide. Ca(OH)2 + SS was the most effective in reducing MIP-1α. CHX was effective in reducing IL-6 and G-CSF. Therefore, the combined intracanal medication of calcium hydroxide and chlorhexidine is effective in reducing the cytokines TNF-α, IL-1β, IL-6, G-CSF and nitric oxide.Department of Bioscience and Oral Diagnosis Institute of Science and Technology São Paulo State University - UNESP, São José dos CamposDepartment of Restorative Dentistry Endodontics division Institute of Science and Technology Saõ Paulo State University - UNESP, Saõ Jose dos CamposDepartment of Bioscience and Oral Diagnosis Institute of Science and Technology São Paulo State University - UNESP, São José dos CamposDepartment of Restorative Dentistry Endodontics division Institute of Science and Technology Saõ Paulo State University - UNESP, Saõ Jose dos CamposUniversidade Estadual Paulista (UNESP)Dias de Oliveira, Luciane [UNESP]de Oliveira, Felipe Eduardo [UNESP]Hatje, Bárbara Araujo [UNESP]Valera, Marcia Carneiro [UNESP]Carvalho, Cláudio Antonio Talge [UNESP]Hasna, Amjad Abu [UNESP]2023-07-29T13:31:44Z2023-07-29T13:31:44Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article36-43http://dx.doi.org/10.1590/0103-6440202205195Brazilian Dental Journal, v. 33, n. 6, p. 36-43, 2022.1806-47600103-6440http://hdl.handle.net/11449/24800210.1590/0103-64402022051952-s2.0-85143566161Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journalinfo:eu-repo/semantics/openAccess2023-07-29T13:31:44Zoai:repositorio.unesp.br:11449/248002Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:31:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production
title Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production
spellingShingle Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production
Dias de Oliveira, Luciane [UNESP]
calcium hydroxide
chlorhexidine
cytokines
lipoteichoic acid
macrophages
title_short Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production
title_full Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production
title_fullStr Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production
title_full_unstemmed Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production
title_sort Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production
author Dias de Oliveira, Luciane [UNESP]
author_facet Dias de Oliveira, Luciane [UNESP]
de Oliveira, Felipe Eduardo [UNESP]
Hatje, Bárbara Araujo [UNESP]
Valera, Marcia Carneiro [UNESP]
Carvalho, Cláudio Antonio Talge [UNESP]
Hasna, Amjad Abu [UNESP]
author_role author
author2 de Oliveira, Felipe Eduardo [UNESP]
Hatje, Bárbara Araujo [UNESP]
Valera, Marcia Carneiro [UNESP]
Carvalho, Cláudio Antonio Talge [UNESP]
Hasna, Amjad Abu [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Dias de Oliveira, Luciane [UNESP]
de Oliveira, Felipe Eduardo [UNESP]
Hatje, Bárbara Araujo [UNESP]
Valera, Marcia Carneiro [UNESP]
Carvalho, Cláudio Antonio Talge [UNESP]
Hasna, Amjad Abu [UNESP]
dc.subject.por.fl_str_mv calcium hydroxide
chlorhexidine
cytokines
lipoteichoic acid
macrophages
topic calcium hydroxide
chlorhexidine
cytokines
lipoteichoic acid
macrophages
description The aim of this study was to evaluate in vitro the effect of calcium hydroxide [Ca(OH)2], 2% chlorhexidine gel (CHX) on macrophages (RAW 264.7) to produce pro-inflammatory cytokines and nitric oxide after pretreatment with lipoteichoic acid (LTA) of Enterococcus faecalis. Forty-eight human single- rooted teeth were instrumented with R25.08 (RECIPROC) and sterilized by gamma irradiation. LTA was inoculated in the root canal of each specimen for 96 hours. Specimens were instrumented with 40.06 and 50.05 (RECIPROC) and medicated with: I) Pyrogen-free saline solution (SS); II) 2% CHX gel; III) Ca(OH)2 + SS; or IV) Ca(OH)2 + CHX for 14 days. Three samples (S) were performed of the root canal of each specimen at: S1) immediately after instrumentation; S2) after Ethylenediaminetetraacetic acid (EDTA); S3) after intracanal medication removal. Subsequent quantification of cytokines (IL-1 β, TNF-α, MIP-1α, IP-10, G-CSF and IL-6) by immunosorbent assay (ELISA) and nitric oxide by the Griess method was carried-out. Data were submitted to a normality test and then analyzed with one-way ANOVA and Tukey test with a significance level of 5% using GraphPad Prism 6. Ca(OH)2 + SS and Ca(OH)2 + CHX presented lower levels of TNF-α, TNF-α, IL-6, G-CSF and nitric oxide. Ca(OH)2 + SS was the most effective in reducing MIP-1α. CHX was effective in reducing IL-6 and G-CSF. Therefore, the combined intracanal medication of calcium hydroxide and chlorhexidine is effective in reducing the cytokines TNF-α, IL-1β, IL-6, G-CSF and nitric oxide.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
2023-07-29T13:31:44Z
2023-07-29T13:31:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0103-6440202205195
Brazilian Dental Journal, v. 33, n. 6, p. 36-43, 2022.
1806-4760
0103-6440
http://hdl.handle.net/11449/248002
10.1590/0103-6440202205195
2-s2.0-85143566161
url http://dx.doi.org/10.1590/0103-6440202205195
http://hdl.handle.net/11449/248002
identifier_str_mv Brazilian Dental Journal, v. 33, n. 6, p. 36-43, 2022.
1806-4760
0103-6440
10.1590/0103-6440202205195
2-s2.0-85143566161
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Dental Journal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 36-43
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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