Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0103-6440202205195 http://hdl.handle.net/11449/248002 |
Resumo: | The aim of this study was to evaluate in vitro the effect of calcium hydroxide [Ca(OH)2], 2% chlorhexidine gel (CHX) on macrophages (RAW 264.7) to produce pro-inflammatory cytokines and nitric oxide after pretreatment with lipoteichoic acid (LTA) of Enterococcus faecalis. Forty-eight human single- rooted teeth were instrumented with R25.08 (RECIPROC) and sterilized by gamma irradiation. LTA was inoculated in the root canal of each specimen for 96 hours. Specimens were instrumented with 40.06 and 50.05 (RECIPROC) and medicated with: I) Pyrogen-free saline solution (SS); II) 2% CHX gel; III) Ca(OH)2 + SS; or IV) Ca(OH)2 + CHX for 14 days. Three samples (S) were performed of the root canal of each specimen at: S1) immediately after instrumentation; S2) after Ethylenediaminetetraacetic acid (EDTA); S3) after intracanal medication removal. Subsequent quantification of cytokines (IL-1 β, TNF-α, MIP-1α, IP-10, G-CSF and IL-6) by immunosorbent assay (ELISA) and nitric oxide by the Griess method was carried-out. Data were submitted to a normality test and then analyzed with one-way ANOVA and Tukey test with a significance level of 5% using GraphPad Prism 6. Ca(OH)2 + SS and Ca(OH)2 + CHX presented lower levels of TNF-α, TNF-α, IL-6, G-CSF and nitric oxide. Ca(OH)2 + SS was the most effective in reducing MIP-1α. CHX was effective in reducing IL-6 and G-CSF. Therefore, the combined intracanal medication of calcium hydroxide and chlorhexidine is effective in reducing the cytokines TNF-α, IL-1β, IL-6, G-CSF and nitric oxide. |
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Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines productioncalcium hydroxidechlorhexidinecytokineslipoteichoic acidmacrophagesThe aim of this study was to evaluate in vitro the effect of calcium hydroxide [Ca(OH)2], 2% chlorhexidine gel (CHX) on macrophages (RAW 264.7) to produce pro-inflammatory cytokines and nitric oxide after pretreatment with lipoteichoic acid (LTA) of Enterococcus faecalis. Forty-eight human single- rooted teeth were instrumented with R25.08 (RECIPROC) and sterilized by gamma irradiation. LTA was inoculated in the root canal of each specimen for 96 hours. Specimens were instrumented with 40.06 and 50.05 (RECIPROC) and medicated with: I) Pyrogen-free saline solution (SS); II) 2% CHX gel; III) Ca(OH)2 + SS; or IV) Ca(OH)2 + CHX for 14 days. Three samples (S) were performed of the root canal of each specimen at: S1) immediately after instrumentation; S2) after Ethylenediaminetetraacetic acid (EDTA); S3) after intracanal medication removal. Subsequent quantification of cytokines (IL-1 β, TNF-α, MIP-1α, IP-10, G-CSF and IL-6) by immunosorbent assay (ELISA) and nitric oxide by the Griess method was carried-out. Data were submitted to a normality test and then analyzed with one-way ANOVA and Tukey test with a significance level of 5% using GraphPad Prism 6. Ca(OH)2 + SS and Ca(OH)2 + CHX presented lower levels of TNF-α, TNF-α, IL-6, G-CSF and nitric oxide. Ca(OH)2 + SS was the most effective in reducing MIP-1α. CHX was effective in reducing IL-6 and G-CSF. Therefore, the combined intracanal medication of calcium hydroxide and chlorhexidine is effective in reducing the cytokines TNF-α, IL-1β, IL-6, G-CSF and nitric oxide.Department of Bioscience and Oral Diagnosis Institute of Science and Technology São Paulo State University - UNESP, São José dos CamposDepartment of Restorative Dentistry Endodontics division Institute of Science and Technology Saõ Paulo State University - UNESP, Saõ Jose dos CamposDepartment of Bioscience and Oral Diagnosis Institute of Science and Technology São Paulo State University - UNESP, São José dos CamposDepartment of Restorative Dentistry Endodontics division Institute of Science and Technology Saõ Paulo State University - UNESP, Saõ Jose dos CamposUniversidade Estadual Paulista (UNESP)Dias de Oliveira, Luciane [UNESP]de Oliveira, Felipe Eduardo [UNESP]Hatje, Bárbara Araujo [UNESP]Valera, Marcia Carneiro [UNESP]Carvalho, Cláudio Antonio Talge [UNESP]Hasna, Amjad Abu [UNESP]2023-07-29T13:31:44Z2023-07-29T13:31:44Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article36-43http://dx.doi.org/10.1590/0103-6440202205195Brazilian Dental Journal, v. 33, n. 6, p. 36-43, 2022.1806-47600103-6440http://hdl.handle.net/11449/24800210.1590/0103-64402022051952-s2.0-85143566161Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journalinfo:eu-repo/semantics/openAccess2023-07-29T13:31:44Zoai:repositorio.unesp.br:11449/248002Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:31:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production |
title |
Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production |
spellingShingle |
Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production Dias de Oliveira, Luciane [UNESP] calcium hydroxide chlorhexidine cytokines lipoteichoic acid macrophages |
title_short |
Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production |
title_full |
Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production |
title_fullStr |
Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production |
title_full_unstemmed |
Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production |
title_sort |
Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production |
author |
Dias de Oliveira, Luciane [UNESP] |
author_facet |
Dias de Oliveira, Luciane [UNESP] de Oliveira, Felipe Eduardo [UNESP] Hatje, Bárbara Araujo [UNESP] Valera, Marcia Carneiro [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] Hasna, Amjad Abu [UNESP] |
author_role |
author |
author2 |
de Oliveira, Felipe Eduardo [UNESP] Hatje, Bárbara Araujo [UNESP] Valera, Marcia Carneiro [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] Hasna, Amjad Abu [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Dias de Oliveira, Luciane [UNESP] de Oliveira, Felipe Eduardo [UNESP] Hatje, Bárbara Araujo [UNESP] Valera, Marcia Carneiro [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] Hasna, Amjad Abu [UNESP] |
dc.subject.por.fl_str_mv |
calcium hydroxide chlorhexidine cytokines lipoteichoic acid macrophages |
topic |
calcium hydroxide chlorhexidine cytokines lipoteichoic acid macrophages |
description |
The aim of this study was to evaluate in vitro the effect of calcium hydroxide [Ca(OH)2], 2% chlorhexidine gel (CHX) on macrophages (RAW 264.7) to produce pro-inflammatory cytokines and nitric oxide after pretreatment with lipoteichoic acid (LTA) of Enterococcus faecalis. Forty-eight human single- rooted teeth were instrumented with R25.08 (RECIPROC) and sterilized by gamma irradiation. LTA was inoculated in the root canal of each specimen for 96 hours. Specimens were instrumented with 40.06 and 50.05 (RECIPROC) and medicated with: I) Pyrogen-free saline solution (SS); II) 2% CHX gel; III) Ca(OH)2 + SS; or IV) Ca(OH)2 + CHX for 14 days. Three samples (S) were performed of the root canal of each specimen at: S1) immediately after instrumentation; S2) after Ethylenediaminetetraacetic acid (EDTA); S3) after intracanal medication removal. Subsequent quantification of cytokines (IL-1 β, TNF-α, MIP-1α, IP-10, G-CSF and IL-6) by immunosorbent assay (ELISA) and nitric oxide by the Griess method was carried-out. Data were submitted to a normality test and then analyzed with one-way ANOVA and Tukey test with a significance level of 5% using GraphPad Prism 6. Ca(OH)2 + SS and Ca(OH)2 + CHX presented lower levels of TNF-α, TNF-α, IL-6, G-CSF and nitric oxide. Ca(OH)2 + SS was the most effective in reducing MIP-1α. CHX was effective in reducing IL-6 and G-CSF. Therefore, the combined intracanal medication of calcium hydroxide and chlorhexidine is effective in reducing the cytokines TNF-α, IL-1β, IL-6, G-CSF and nitric oxide. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-07-29T13:31:44Z 2023-07-29T13:31:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0103-6440202205195 Brazilian Dental Journal, v. 33, n. 6, p. 36-43, 2022. 1806-4760 0103-6440 http://hdl.handle.net/11449/248002 10.1590/0103-6440202205195 2-s2.0-85143566161 |
url |
http://dx.doi.org/10.1590/0103-6440202205195 http://hdl.handle.net/11449/248002 |
identifier_str_mv |
Brazilian Dental Journal, v. 33, n. 6, p. 36-43, 2022. 1806-4760 0103-6440 10.1590/0103-6440202205195 2-s2.0-85143566161 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
36-43 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965211381202944 |