Development of HA/Ag-NPs composite coating from green process for hip applications

Detalhes bibliográficos
Autor(a) principal: Lozoya-Rodrfguez, Denisse A.
Data de Publicação: 2017
Outros Autores: De Lima, Renata, Fraceto, Leonardo F. [UNESP], Pérez, Antonio Ledezma, Domfnguez, Mercedes Bazaldua, Batres, Roberto Gómez, Rojas, Armando Reyes, Carmona, Víctor Orozco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/molecules22081291
http://hdl.handle.net/11449/179127
Resumo: In the present study, biological hydroxyapatite (HA) was obtained from bovine bones through a thermal process. A total of 0% and 1% of silver nanoparticles (Ag-NPs) synthesized from Opuntia ficus (nopal) were added to the biological hydroxyapatite coatings using an atmospheric plasma spray (APS) on a Ti6Al4V substrate. Following this, its antimicrobial efficiency was evaluated against the following bacterial strains: Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. This was conducted according to the Japanese Industrial Standard (JIS) Z2801:2000 Antimicrobial Product-Test for Antimicrobial Activity and Efficacy. Scanning electron microscopy (SEM) showed that the silver nanoparticles (Ag-NPs) were evenly distributed on the coating surface. Energy dispersive X-ray spectroscopy (EDX) shows that apatite deposition occurs on a daily basis, maintaining a Ca/P rate between 2.12 and 1.45. Biocompatibility properties were evaluated with osteoblast-like cells (MC3T3-E1) by single-cell gel electrophoresis assay and Tali image cytometry.
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spelling Development of HA/Ag-NPs composite coating from green process for hip applicationsAntibacterial testAtmospheric plasma sprayBiological hydroxyapatiteCoatingCytotoxic testSilver nanoparticlesIn the present study, biological hydroxyapatite (HA) was obtained from bovine bones through a thermal process. A total of 0% and 1% of silver nanoparticles (Ag-NPs) synthesized from Opuntia ficus (nopal) were added to the biological hydroxyapatite coatings using an atmospheric plasma spray (APS) on a Ti6Al4V substrate. Following this, its antimicrobial efficiency was evaluated against the following bacterial strains: Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. This was conducted according to the Japanese Industrial Standard (JIS) Z2801:2000 Antimicrobial Product-Test for Antimicrobial Activity and Efficacy. Scanning electron microscopy (SEM) showed that the silver nanoparticles (Ag-NPs) were evenly distributed on the coating surface. Energy dispersive X-ray spectroscopy (EDX) shows that apatite deposition occurs on a daily basis, maintaining a Ca/P rate between 2.12 and 1.45. Biocompatibility properties were evaluated with osteoblast-like cells (MC3T3-E1) by single-cell gel electrophoresis assay and Tali image cytometry.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Centro de Investigación en Materiales Avanzados (CIMAV), Miguel de Cervantes 120Departamento de Biotecnologia Universidade de Sorocaba (UNISO), Rod. Raposo Tavares, km 92.5Departamento de Engenharia Ambiental Universidade Estadual Paulista (UNESP), Av. Très de Março 511Centro de Investigación en Quîmica Aplicada (CIQA), Blvd. Enrique Reyna 140Centro de Capacitacion Investigacion Innovacion y Transferencia Tecnologica Universidad Tecnológica de Chihuahua Sur (UTCh Sur), Km. 3 Carretera Chihuahua-AldamaDepartamento de Engenharia Ambiental Universidade Estadual Paulista (UNESP), Av. Très de Março 511Centro de Investigación en Materiales Avanzados (CIMAV)Universidade de Sorocaba (UNISO)Universidade Estadual Paulista (Unesp)Centro de Investigación en Quîmica Aplicada (CIQA)Universidad Tecnológica de Chihuahua Sur (UTCh Sur)Lozoya-Rodrfguez, Denisse A.De Lima, RenataFraceto, Leonardo F. [UNESP]Pérez, Antonio LedezmaDomfnguez, Mercedes BazalduaBatres, Roberto GómezRojas, Armando ReyesCarmona, Víctor Orozco2018-12-11T17:33:51Z2018-12-11T17:33:51Z2017-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.3390/molecules22081291Molecules, v. 22, n. 8, 2017.1420-3049http://hdl.handle.net/11449/17912710.3390/molecules220812912-s2.0-850283581382-s2.0-85028358138.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecules0,855info:eu-repo/semantics/openAccess2024-01-21T06:22:37Zoai:repositorio.unesp.br:11449/179127Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-05-23T21:19:17.728662Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Development of HA/Ag-NPs composite coating from green process for hip applications
title Development of HA/Ag-NPs composite coating from green process for hip applications
spellingShingle Development of HA/Ag-NPs composite coating from green process for hip applications
Lozoya-Rodrfguez, Denisse A.
Antibacterial test
Atmospheric plasma spray
Biological hydroxyapatite
Coating
Cytotoxic test
Silver nanoparticles
title_short Development of HA/Ag-NPs composite coating from green process for hip applications
title_full Development of HA/Ag-NPs composite coating from green process for hip applications
title_fullStr Development of HA/Ag-NPs composite coating from green process for hip applications
title_full_unstemmed Development of HA/Ag-NPs composite coating from green process for hip applications
title_sort Development of HA/Ag-NPs composite coating from green process for hip applications
author Lozoya-Rodrfguez, Denisse A.
author_facet Lozoya-Rodrfguez, Denisse A.
De Lima, Renata
Fraceto, Leonardo F. [UNESP]
Pérez, Antonio Ledezma
Domfnguez, Mercedes Bazaldua
Batres, Roberto Gómez
Rojas, Armando Reyes
Carmona, Víctor Orozco
author_role author
author2 De Lima, Renata
Fraceto, Leonardo F. [UNESP]
Pérez, Antonio Ledezma
Domfnguez, Mercedes Bazaldua
Batres, Roberto Gómez
Rojas, Armando Reyes
Carmona, Víctor Orozco
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Investigación en Materiales Avanzados (CIMAV)
Universidade de Sorocaba (UNISO)
Universidade Estadual Paulista (Unesp)
Centro de Investigación en Quîmica Aplicada (CIQA)
Universidad Tecnológica de Chihuahua Sur (UTCh Sur)
dc.contributor.author.fl_str_mv Lozoya-Rodrfguez, Denisse A.
De Lima, Renata
Fraceto, Leonardo F. [UNESP]
Pérez, Antonio Ledezma
Domfnguez, Mercedes Bazaldua
Batres, Roberto Gómez
Rojas, Armando Reyes
Carmona, Víctor Orozco
dc.subject.por.fl_str_mv Antibacterial test
Atmospheric plasma spray
Biological hydroxyapatite
Coating
Cytotoxic test
Silver nanoparticles
topic Antibacterial test
Atmospheric plasma spray
Biological hydroxyapatite
Coating
Cytotoxic test
Silver nanoparticles
description In the present study, biological hydroxyapatite (HA) was obtained from bovine bones through a thermal process. A total of 0% and 1% of silver nanoparticles (Ag-NPs) synthesized from Opuntia ficus (nopal) were added to the biological hydroxyapatite coatings using an atmospheric plasma spray (APS) on a Ti6Al4V substrate. Following this, its antimicrobial efficiency was evaluated against the following bacterial strains: Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. This was conducted according to the Japanese Industrial Standard (JIS) Z2801:2000 Antimicrobial Product-Test for Antimicrobial Activity and Efficacy. Scanning electron microscopy (SEM) showed that the silver nanoparticles (Ag-NPs) were evenly distributed on the coating surface. Energy dispersive X-ray spectroscopy (EDX) shows that apatite deposition occurs on a daily basis, maintaining a Ca/P rate between 2.12 and 1.45. Biocompatibility properties were evaluated with osteoblast-like cells (MC3T3-E1) by single-cell gel electrophoresis assay and Tali image cytometry.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-01
2018-12-11T17:33:51Z
2018-12-11T17:33:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/molecules22081291
Molecules, v. 22, n. 8, 2017.
1420-3049
http://hdl.handle.net/11449/179127
10.3390/molecules22081291
2-s2.0-85028358138
2-s2.0-85028358138.pdf
url http://dx.doi.org/10.3390/molecules22081291
http://hdl.handle.net/11449/179127
identifier_str_mv Molecules, v. 22, n. 8, 2017.
1420-3049
10.3390/molecules22081291
2-s2.0-85028358138
2-s2.0-85028358138.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Molecules
0,855
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1803045737220538368

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