Use of molecular markers can help to understand the genetic diversity of Babesia bovis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.meegid.2019.104161 http://hdl.handle.net/11449/197660 |
Resumo: | Cattle babesiosis is a tick-borne disease responsible for significant losses for the livestock industries in tropical areas of the world. These piroplasms are under constant control of the host immune system, which lead to a strong selective pressure for arising more virulent or attenuated phenotypes. Aiming to better understand the most critical genetic modifications in Babesia bovis genome, related to virulence, an in silico analysis was performed using DNA sequences from GenBank. Fourteen genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80 (or Bb-1), 18S rRNA, acs-1, ama-1, beta-tub, cp-2, p0, rap-1a) related to parasite infection and immunogenicity and ITS region were selected for alignment and comparison of several isolates of Babesia bovis from different geographic regions around the world. Among the 15 genes selected for the study of diversity, only 7 genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80) and the ITS region presented sufficient genetic variation for the studies of phylogeny. Despite this genetic diversity observed into groups, there was not sufficient information available to associate molecular markers with virulence of isolates. However, some genetic groups no were correlated with geographic region what could indicate some typical evolutionary characteristics in the relation between parasitehost. Further studies using these genes in herds presenting diverse clinical conditions are required. The better understanding of evolutionary mechanisms of the parasite may contribute to improve prophylactic and therapeutic measures. In this way, we suggest that genes used in our study are potential markers of virulence and attenuation and have to be analyzed with the use of sequences from animals that present clinical signs of babesiosis and asymptomatic carriers. |
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Use of molecular markers can help to understand the genetic diversity of Babesia bovisBabesia bovisMolecular markersDiversityVirulenceAttenuationCattle babesiosis is a tick-borne disease responsible for significant losses for the livestock industries in tropical areas of the world. These piroplasms are under constant control of the host immune system, which lead to a strong selective pressure for arising more virulent or attenuated phenotypes. Aiming to better understand the most critical genetic modifications in Babesia bovis genome, related to virulence, an in silico analysis was performed using DNA sequences from GenBank. Fourteen genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80 (or Bb-1), 18S rRNA, acs-1, ama-1, beta-tub, cp-2, p0, rap-1a) related to parasite infection and immunogenicity and ITS region were selected for alignment and comparison of several isolates of Babesia bovis from different geographic regions around the world. Among the 15 genes selected for the study of diversity, only 7 genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80) and the ITS region presented sufficient genetic variation for the studies of phylogeny. Despite this genetic diversity observed into groups, there was not sufficient information available to associate molecular markers with virulence of isolates. However, some genetic groups no were correlated with geographic region what could indicate some typical evolutionary characteristics in the relation between parasitehost. Further studies using these genes in herds presenting diverse clinical conditions are required. The better understanding of evolutionary mechanisms of the parasite may contribute to improve prophylactic and therapeutic measures. In this way, we suggest that genes used in our study are potential markers of virulence and attenuation and have to be analyzed with the use of sequences from animals that present clinical signs of babesiosis and asymptomatic carriers.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Brazilian Agricultural Research CorporationUniv Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, SP, BrazilEmbrapa Pecuaria Sudeste, Sao Carlos, SP, BrazilInst Zootecnia, Ctr Pesquisa Genet & Reprod Anim, Nova Odessa, SP, BrazilWashington Luiz Rd,Km 234, BR-13560970 Sao Carlos, SP, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, SP, BrazilFAPESP: 2016/07216-7FAPESP: 2018/16582-2CNPq: 153231/2018-1Brazilian Agricultural Research Corporation: Embrapa SEG 02.12.02.008.00.00Elsevier B.V.Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Inst ZootecniaMarques Simas, Paulo Vitor [UNESP]Bassetto, Cesar Cristiano [UNESP]Giglioti, RodrigoOkino, Cintia HiromiOliveira, Henrique Nunes de [UNESP]Sena Oliveira, Marcia Cristina de2020-12-11T07:55:45Z2020-12-11T07:55:45Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9http://dx.doi.org/10.1016/j.meegid.2019.104161Infection Genetics And Evolution. Amsterdam: Elsevier, v. 79, 9 p., 2020.1567-1348http://hdl.handle.net/11449/19766010.1016/j.meegid.2019.104161WOS:000510866400011Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInfection Genetics And Evolutioninfo:eu-repo/semantics/openAccess2021-10-23T10:18:32Zoai:repositorio.unesp.br:11449/197660Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T10:18:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Use of molecular markers can help to understand the genetic diversity of Babesia bovis |
title |
Use of molecular markers can help to understand the genetic diversity of Babesia bovis |
spellingShingle |
Use of molecular markers can help to understand the genetic diversity of Babesia bovis Marques Simas, Paulo Vitor [UNESP] Babesia bovis Molecular markers Diversity Virulence Attenuation |
title_short |
Use of molecular markers can help to understand the genetic diversity of Babesia bovis |
title_full |
Use of molecular markers can help to understand the genetic diversity of Babesia bovis |
title_fullStr |
Use of molecular markers can help to understand the genetic diversity of Babesia bovis |
title_full_unstemmed |
Use of molecular markers can help to understand the genetic diversity of Babesia bovis |
title_sort |
Use of molecular markers can help to understand the genetic diversity of Babesia bovis |
author |
Marques Simas, Paulo Vitor [UNESP] |
author_facet |
Marques Simas, Paulo Vitor [UNESP] Bassetto, Cesar Cristiano [UNESP] Giglioti, Rodrigo Okino, Cintia Hiromi Oliveira, Henrique Nunes de [UNESP] Sena Oliveira, Marcia Cristina de |
author_role |
author |
author2 |
Bassetto, Cesar Cristiano [UNESP] Giglioti, Rodrigo Okino, Cintia Hiromi Oliveira, Henrique Nunes de [UNESP] Sena Oliveira, Marcia Cristina de |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Inst Zootecnia |
dc.contributor.author.fl_str_mv |
Marques Simas, Paulo Vitor [UNESP] Bassetto, Cesar Cristiano [UNESP] Giglioti, Rodrigo Okino, Cintia Hiromi Oliveira, Henrique Nunes de [UNESP] Sena Oliveira, Marcia Cristina de |
dc.subject.por.fl_str_mv |
Babesia bovis Molecular markers Diversity Virulence Attenuation |
topic |
Babesia bovis Molecular markers Diversity Virulence Attenuation |
description |
Cattle babesiosis is a tick-borne disease responsible for significant losses for the livestock industries in tropical areas of the world. These piroplasms are under constant control of the host immune system, which lead to a strong selective pressure for arising more virulent or attenuated phenotypes. Aiming to better understand the most critical genetic modifications in Babesia bovis genome, related to virulence, an in silico analysis was performed using DNA sequences from GenBank. Fourteen genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80 (or Bb-1), 18S rRNA, acs-1, ama-1, beta-tub, cp-2, p0, rap-1a) related to parasite infection and immunogenicity and ITS region were selected for alignment and comparison of several isolates of Babesia bovis from different geographic regions around the world. Among the 15 genes selected for the study of diversity, only 7 genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80) and the ITS region presented sufficient genetic variation for the studies of phylogeny. Despite this genetic diversity observed into groups, there was not sufficient information available to associate molecular markers with virulence of isolates. However, some genetic groups no were correlated with geographic region what could indicate some typical evolutionary characteristics in the relation between parasitehost. Further studies using these genes in herds presenting diverse clinical conditions are required. The better understanding of evolutionary mechanisms of the parasite may contribute to improve prophylactic and therapeutic measures. In this way, we suggest that genes used in our study are potential markers of virulence and attenuation and have to be analyzed with the use of sequences from animals that present clinical signs of babesiosis and asymptomatic carriers. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-11T07:55:45Z 2020-12-11T07:55:45Z 2020-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.meegid.2019.104161 Infection Genetics And Evolution. Amsterdam: Elsevier, v. 79, 9 p., 2020. 1567-1348 http://hdl.handle.net/11449/197660 10.1016/j.meegid.2019.104161 WOS:000510866400011 |
url |
http://dx.doi.org/10.1016/j.meegid.2019.104161 http://hdl.handle.net/11449/197660 |
identifier_str_mv |
Infection Genetics And Evolution. Amsterdam: Elsevier, v. 79, 9 p., 2020. 1567-1348 10.1016/j.meegid.2019.104161 WOS:000510866400011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Infection Genetics And Evolution |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965546471489536 |