Black Tea Extract and Its Theaflavin Derivatives Inhibit the Growth of Periodontopathogens and Modulate Interleukin-8 and beta-Defensin Secretion in Oral Epithelial Cells
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0143158 http://hdl.handle.net/11449/160985 |
Resumo: | Over the years, several studies have brought evidence suggesting that tea polyphenols, mostly from green tea, may have oral health benefits. Since few data are available concerning the beneficial properties of black tea and its theaflavin derivatives against periodontal disease, the objective of this study was to investigate their antibacterial activity as well as their ability to modulate interleukin-8 and human beta-defensin (hBD) secretion in oral epithelial cells. Among the periodontopathogenic bacteria tested, Porphyromonas gingivalis was found to be highly susceptible to the black tea extract and theaflavins. Moreover, our data indicated that the black tea extract, theaflavin and theaflavin-3,3'-digallate can potentiate the antibacterial effect of metronidazole and tetracycline against P. gingivalis. Using lipopolysaccharide-stimulated oral epithelial cells, the black tea extract (100 mu g/ml), as well as theaflavin and theaflavin-3,3'-digallate (50 mu g/ml) reduced interleukin-8 (IL-8) secretion by 85%, 79%, and 86%, respectively, thus suggesting an anti-inflammatory property. The ability of the black tea extract and its theaflavin derivatives to induce the secretion of the antimicrobial peptides hBD-1, hBD-2 and hBD-4 by oral epithelial cells was then evaluated. Our results showed that the black tea extract as well as theaflavin-3,3'-digallate were able to increase the secretion of the three hBDs. In conclusion, the ability of a black tea extract and theaflavins to exert antibacterial activity against major periodontopathogens, to attenuate the secretion of IL-8, and to induce hBD secretion in oral epithelial cells suggest that these components may have a beneficial effect against periodontal disease. |
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Black Tea Extract and Its Theaflavin Derivatives Inhibit the Growth of Periodontopathogens and Modulate Interleukin-8 and beta-Defensin Secretion in Oral Epithelial CellsOver the years, several studies have brought evidence suggesting that tea polyphenols, mostly from green tea, may have oral health benefits. Since few data are available concerning the beneficial properties of black tea and its theaflavin derivatives against periodontal disease, the objective of this study was to investigate their antibacterial activity as well as their ability to modulate interleukin-8 and human beta-defensin (hBD) secretion in oral epithelial cells. Among the periodontopathogenic bacteria tested, Porphyromonas gingivalis was found to be highly susceptible to the black tea extract and theaflavins. Moreover, our data indicated that the black tea extract, theaflavin and theaflavin-3,3'-digallate can potentiate the antibacterial effect of metronidazole and tetracycline against P. gingivalis. Using lipopolysaccharide-stimulated oral epithelial cells, the black tea extract (100 mu g/ml), as well as theaflavin and theaflavin-3,3'-digallate (50 mu g/ml) reduced interleukin-8 (IL-8) secretion by 85%, 79%, and 86%, respectively, thus suggesting an anti-inflammatory property. The ability of the black tea extract and its theaflavin derivatives to induce the secretion of the antimicrobial peptides hBD-1, hBD-2 and hBD-4 by oral epithelial cells was then evaluated. Our results showed that the black tea extract as well as theaflavin-3,3'-digallate were able to increase the secretion of the three hBDs. In conclusion, the ability of a black tea extract and theaflavins to exert antibacterial activity against major periodontopathogens, to attenuate the secretion of IL-8, and to induce hBD secretion in oral epithelial cells suggest that these components may have a beneficial effect against periodontal disease.Laboratoire de Controle MicrobiologiqueUNINOVE Univ, Sao Paulo Dent Sch, Sao Paulo, BrazilUniv Laval, Fac Dent, Oral Ecol Res Grp, Quebec City, PQ, CanadaState Univ Sao Paulo, Araraquara Dent Sch, Dept Oral Diag & Surg, Sao Paulo, BrazilState Univ Sao Paulo, Araraquara Dent Sch, Dept Oral Diag & Surg, Sao Paulo, BrazilPublic Library ScienceUNINOVE UnivUniv LavalUniversidade Estadual Paulista (Unesp)Lombardo Bedran, Telma BlancaMorin, Marie-PierreSpolidorio, Denise Palomari [UNESP]Grenier, Daniel2018-11-26T16:17:30Z2018-11-26T16:17:30Z2015-11-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://dx.doi.org/10.1371/journal.pone.0143158Plos One. San Francisco: Public Library Science, v. 10, n. 11, 11 p., 2015.1932-6203http://hdl.handle.net/11449/16098510.1371/journal.pone.0143158WOS:000365154600093WOS000365154600093.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One1,164info:eu-repo/semantics/openAccess2023-12-06T06:18:22Zoai:repositorio.unesp.br:11449/160985Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-06T06:18:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Black Tea Extract and Its Theaflavin Derivatives Inhibit the Growth of Periodontopathogens and Modulate Interleukin-8 and beta-Defensin Secretion in Oral Epithelial Cells |
title |
Black Tea Extract and Its Theaflavin Derivatives Inhibit the Growth of Periodontopathogens and Modulate Interleukin-8 and beta-Defensin Secretion in Oral Epithelial Cells |
spellingShingle |
Black Tea Extract and Its Theaflavin Derivatives Inhibit the Growth of Periodontopathogens and Modulate Interleukin-8 and beta-Defensin Secretion in Oral Epithelial Cells Lombardo Bedran, Telma Blanca |
title_short |
Black Tea Extract and Its Theaflavin Derivatives Inhibit the Growth of Periodontopathogens and Modulate Interleukin-8 and beta-Defensin Secretion in Oral Epithelial Cells |
title_full |
Black Tea Extract and Its Theaflavin Derivatives Inhibit the Growth of Periodontopathogens and Modulate Interleukin-8 and beta-Defensin Secretion in Oral Epithelial Cells |
title_fullStr |
Black Tea Extract and Its Theaflavin Derivatives Inhibit the Growth of Periodontopathogens and Modulate Interleukin-8 and beta-Defensin Secretion in Oral Epithelial Cells |
title_full_unstemmed |
Black Tea Extract and Its Theaflavin Derivatives Inhibit the Growth of Periodontopathogens and Modulate Interleukin-8 and beta-Defensin Secretion in Oral Epithelial Cells |
title_sort |
Black Tea Extract and Its Theaflavin Derivatives Inhibit the Growth of Periodontopathogens and Modulate Interleukin-8 and beta-Defensin Secretion in Oral Epithelial Cells |
author |
Lombardo Bedran, Telma Blanca |
author_facet |
Lombardo Bedran, Telma Blanca Morin, Marie-Pierre Spolidorio, Denise Palomari [UNESP] Grenier, Daniel |
author_role |
author |
author2 |
Morin, Marie-Pierre Spolidorio, Denise Palomari [UNESP] Grenier, Daniel |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
UNINOVE Univ Univ Laval Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Lombardo Bedran, Telma Blanca Morin, Marie-Pierre Spolidorio, Denise Palomari [UNESP] Grenier, Daniel |
description |
Over the years, several studies have brought evidence suggesting that tea polyphenols, mostly from green tea, may have oral health benefits. Since few data are available concerning the beneficial properties of black tea and its theaflavin derivatives against periodontal disease, the objective of this study was to investigate their antibacterial activity as well as their ability to modulate interleukin-8 and human beta-defensin (hBD) secretion in oral epithelial cells. Among the periodontopathogenic bacteria tested, Porphyromonas gingivalis was found to be highly susceptible to the black tea extract and theaflavins. Moreover, our data indicated that the black tea extract, theaflavin and theaflavin-3,3'-digallate can potentiate the antibacterial effect of metronidazole and tetracycline against P. gingivalis. Using lipopolysaccharide-stimulated oral epithelial cells, the black tea extract (100 mu g/ml), as well as theaflavin and theaflavin-3,3'-digallate (50 mu g/ml) reduced interleukin-8 (IL-8) secretion by 85%, 79%, and 86%, respectively, thus suggesting an anti-inflammatory property. The ability of the black tea extract and its theaflavin derivatives to induce the secretion of the antimicrobial peptides hBD-1, hBD-2 and hBD-4 by oral epithelial cells was then evaluated. Our results showed that the black tea extract as well as theaflavin-3,3'-digallate were able to increase the secretion of the three hBDs. In conclusion, the ability of a black tea extract and theaflavins to exert antibacterial activity against major periodontopathogens, to attenuate the secretion of IL-8, and to induce hBD secretion in oral epithelial cells suggest that these components may have a beneficial effect against periodontal disease. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-11-18 2018-11-26T16:17:30Z 2018-11-26T16:17:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0143158 Plos One. San Francisco: Public Library Science, v. 10, n. 11, 11 p., 2015. 1932-6203 http://hdl.handle.net/11449/160985 10.1371/journal.pone.0143158 WOS:000365154600093 WOS000365154600093.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0143158 http://hdl.handle.net/11449/160985 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 10, n. 11, 11 p., 2015. 1932-6203 10.1371/journal.pone.0143158 WOS:000365154600093 WOS000365154600093.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One 1,164 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1803649914148749312 |