CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-7757201300004 http://hdl.handle.net/11449/74738 |
Resumo: | Objective: The aim of this study was to compare the production of the chemokines CCL3 and CXCL12 by cultured dental pulp fibroblasts from permanent (PDPF) and deciduous (DDPF) teeth under stimulation by Porphyromonas gingivalis LPS (PgLPS). Material and Methods: Primary culture of fibroblasts from permanent (n=3) and deciduous (n=2) teeth were established using an explant technique. After the fourth passage, fibroblasts were stimulated by increasing concentrations of PgLPS (0 - 10 pg/mL) at 1, 6 and 24 h. The cells were tested for viability through MTT assay, and production of the chemokines CCL3 and CXCL12 was determined through ELISA. Comparisons among samples were performed using One-way ANOVA for MTT assay and Two-way ANOVA for ELISA results. Results: Cell viability was not affected by the antigen after 24 h of stimulation. PgLPS induced the production of CCL3 by dental pulp fibroblasts at similar levels for both permanent and deciduous pulp fibroblasts. Production of CXCL12, however, was significantly higher for PDPF than DDPF at 1 and 6 h. PgLPS, in turn, downregulated the production of CXCL12 by PDPF but not by DDPF. Conclusion: These data suggest that dental pulp fibroblasts from permanent and deciduous teeth may present a differential behavior under PgLPS stimulation. |
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CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPSCCL3 chemokineChemokinesCXCL12 chemokineDental pulpDental pulp inflammationFibroblastsObjective: The aim of this study was to compare the production of the chemokines CCL3 and CXCL12 by cultured dental pulp fibroblasts from permanent (PDPF) and deciduous (DDPF) teeth under stimulation by Porphyromonas gingivalis LPS (PgLPS). Material and Methods: Primary culture of fibroblasts from permanent (n=3) and deciduous (n=2) teeth were established using an explant technique. After the fourth passage, fibroblasts were stimulated by increasing concentrations of PgLPS (0 - 10 pg/mL) at 1, 6 and 24 h. The cells were tested for viability through MTT assay, and production of the chemokines CCL3 and CXCL12 was determined through ELISA. Comparisons among samples were performed using One-way ANOVA for MTT assay and Two-way ANOVA for ELISA results. Results: Cell viability was not affected by the antigen after 24 h of stimulation. PgLPS induced the production of CCL3 by dental pulp fibroblasts at similar levels for both permanent and deciduous pulp fibroblasts. Production of CXCL12, however, was significantly higher for PDPF than DDPF at 1 and 6 h. PgLPS, in turn, downregulated the production of CXCL12 by PDPF but not by DDPF. Conclusion: These data suggest that dental pulp fibroblasts from permanent and deciduous teeth may present a differential behavior under PgLPS stimulation.Department of Biological Sciences Bauru School of Dentistry University of São Paulo, Bauru, SPDepartment Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry, University of São Paulo, Bauru, SPDepartment of Pediatric Dentistry, Orthodontics and Community Health Bauru School of Dentistry University of São Paulo, Bauru, SPDepartment of Basic Sciences Araçatuba School of Dentistry Univ. Estadual Paulista - UNESP, Araçatuba, SPDepartment of Basic Sciences Araçatuba School of Dentistry Univ. Estadual Paulista - UNESP, Araçatuba, SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Sipert, Carla RenataMorandini, Ana Carolina de FariaModena, Karin Cristina da SilvaDionisio, Thiago JoseMachado, Maria Aparecida Andrade MoreiraOliveira, Sandra Helena Penha de [UNESP]Campanelli, Ana PaulaSantos, Carlos Ferreira2014-05-27T11:28:36Z2014-05-27T11:28:36Z2013-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article99-105application/pdfhttp://dx.doi.org/10.1590/1678-7757201300004Journal of Applied Oral Science, v. 21, n. 2, p. 99-105, 2013.1678-77571678-7765http://hdl.handle.net/11449/7473810.1590/1678-77572013000041678-7757201300004WOS:0003199977000022-s2.0-848781686542-s2.0-84878168654.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Science1.7090,645info:eu-repo/semantics/openAccess2024-09-19T14:03:16Zoai:repositorio.unesp.br:11449/74738Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T14:03:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS |
title |
CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS |
spellingShingle |
CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS Sipert, Carla Renata CCL3 chemokine Chemokines CXCL12 chemokine Dental pulp Dental pulp inflammation Fibroblasts |
title_short |
CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS |
title_full |
CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS |
title_fullStr |
CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS |
title_full_unstemmed |
CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS |
title_sort |
CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS |
author |
Sipert, Carla Renata |
author_facet |
Sipert, Carla Renata Morandini, Ana Carolina de Faria Modena, Karin Cristina da Silva Dionisio, Thiago Jose Machado, Maria Aparecida Andrade Moreira Oliveira, Sandra Helena Penha de [UNESP] Campanelli, Ana Paula Santos, Carlos Ferreira |
author_role |
author |
author2 |
Morandini, Ana Carolina de Faria Modena, Karin Cristina da Silva Dionisio, Thiago Jose Machado, Maria Aparecida Andrade Moreira Oliveira, Sandra Helena Penha de [UNESP] Campanelli, Ana Paula Santos, Carlos Ferreira |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Sipert, Carla Renata Morandini, Ana Carolina de Faria Modena, Karin Cristina da Silva Dionisio, Thiago Jose Machado, Maria Aparecida Andrade Moreira Oliveira, Sandra Helena Penha de [UNESP] Campanelli, Ana Paula Santos, Carlos Ferreira |
dc.subject.por.fl_str_mv |
CCL3 chemokine Chemokines CXCL12 chemokine Dental pulp Dental pulp inflammation Fibroblasts |
topic |
CCL3 chemokine Chemokines CXCL12 chemokine Dental pulp Dental pulp inflammation Fibroblasts |
description |
Objective: The aim of this study was to compare the production of the chemokines CCL3 and CXCL12 by cultured dental pulp fibroblasts from permanent (PDPF) and deciduous (DDPF) teeth under stimulation by Porphyromonas gingivalis LPS (PgLPS). Material and Methods: Primary culture of fibroblasts from permanent (n=3) and deciduous (n=2) teeth were established using an explant technique. After the fourth passage, fibroblasts were stimulated by increasing concentrations of PgLPS (0 - 10 pg/mL) at 1, 6 and 24 h. The cells were tested for viability through MTT assay, and production of the chemokines CCL3 and CXCL12 was determined through ELISA. Comparisons among samples were performed using One-way ANOVA for MTT assay and Two-way ANOVA for ELISA results. Results: Cell viability was not affected by the antigen after 24 h of stimulation. PgLPS induced the production of CCL3 by dental pulp fibroblasts at similar levels for both permanent and deciduous pulp fibroblasts. Production of CXCL12, however, was significantly higher for PDPF than DDPF at 1 and 6 h. PgLPS, in turn, downregulated the production of CXCL12 by PDPF but not by DDPF. Conclusion: These data suggest that dental pulp fibroblasts from permanent and deciduous teeth may present a differential behavior under PgLPS stimulation. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-01 2014-05-27T11:28:36Z 2014-05-27T11:28:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-7757201300004 Journal of Applied Oral Science, v. 21, n. 2, p. 99-105, 2013. 1678-7757 1678-7765 http://hdl.handle.net/11449/74738 10.1590/1678-7757201300004 1678-7757201300004 WOS:000319997700002 2-s2.0-84878168654 2-s2.0-84878168654.pdf |
url |
http://dx.doi.org/10.1590/1678-7757201300004 http://hdl.handle.net/11449/74738 |
identifier_str_mv |
Journal of Applied Oral Science, v. 21, n. 2, p. 99-105, 2013. 1678-7757 1678-7765 10.1590/1678-7757201300004 1678-7757201300004 WOS:000319997700002 2-s2.0-84878168654 2-s2.0-84878168654.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Oral Science 1.709 0,645 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
99-105 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546538528407552 |