Shelter dogs as indicators for Trypanosoma cruzi infection in an urban area of Aracaju, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.actatropica.2020.105577 http://hdl.handle.net/11449/201885 |
Resumo: | Shelters are places that receive certain species of domestic animals, mostly dogs and cats, that are normally abandoned. These animals are easy to handle because they are crowded and have limited movement. Dogs, due to the close relationship with humans, are considered the main domestic reservoirs in the peridomiciliary cycle of American trypanosomiasis infection in humans. Therefore, in several countries studies are carried out to assess the occurrence of infection in these animals. This work evaluated the occurrence of Trypanosoma cruzi in dogs from shelters in the city of Aracaju, Sergipe. This was an observational and cross-sectional study to detect the occurrence of T. cruzi in blood samples from shelter dogs using polymerase chain reaction (PCR) with the TcZ1/TcZ2 primers. A total of 168 blood samples from dogs (104 females and 64 males) were collected and subjected to DNA and PCR extraction, and seventeen (10.1%) samples showed amplification of the product of the expected size. The TcZ1/TcZ2 primer pair used is considered specific for T. cruzi, and it is capable of amplifying all strains without amplifying other Trypanosoma species. The shelters evaluated had different physical infrastructure; animals positive for T. cruzi were found in all of them, as confirmed by sequencing. It is concluded that shelter dogs, as well as other domestic animals, can function as indicators of the occurrence of T. cruzi in Aracaju-SE, providing relevant epidemiological information for health surveillance and monitoring of Trypanosomatid infections. |
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Shelter dogs as indicators for Trypanosoma cruzi infection in an urban area of Aracaju, BrazilChagas DiseaseDisease reservoirsIndicatorsTrypanosoma cruziShelters are places that receive certain species of domestic animals, mostly dogs and cats, that are normally abandoned. These animals are easy to handle because they are crowded and have limited movement. Dogs, due to the close relationship with humans, are considered the main domestic reservoirs in the peridomiciliary cycle of American trypanosomiasis infection in humans. Therefore, in several countries studies are carried out to assess the occurrence of infection in these animals. This work evaluated the occurrence of Trypanosoma cruzi in dogs from shelters in the city of Aracaju, Sergipe. This was an observational and cross-sectional study to detect the occurrence of T. cruzi in blood samples from shelter dogs using polymerase chain reaction (PCR) with the TcZ1/TcZ2 primers. A total of 168 blood samples from dogs (104 females and 64 males) were collected and subjected to DNA and PCR extraction, and seventeen (10.1%) samples showed amplification of the product of the expected size. The TcZ1/TcZ2 primer pair used is considered specific for T. cruzi, and it is capable of amplifying all strains without amplifying other Trypanosoma species. The shelters evaluated had different physical infrastructure; animals positive for T. cruzi were found in all of them, as confirmed by sequencing. It is concluded that shelter dogs, as well as other domestic animals, can function as indicators of the occurrence of T. cruzi in Aracaju-SE, providing relevant epidemiological information for health surveillance and monitoring of Trypanosomatid infections.Postgraduate Program in Health and Environment Tiradentes University-UNIT, Av. Murilo Dantas, 300Biological Sciences Course Tiradentes University-UNIT, Av. Murilo Dantas, 300Institute of Technology and Research – ITP, Av. Murilo Dantas, 300Postgraduate Program in Bioscience and Biotechnology Apllied to Pharmacy São Paulo State University-UNESP School of Pharmaceutical Sciences Rodovia Araraquara-Jaú, km 1, Campos VillePostgraduate Program in Bioscience and Biotechnology Apllied to Pharmacy São Paulo State University-UNESP School of Pharmaceutical Sciences Rodovia Araraquara-Jaú, km 1, Campos VilleTiradentes University-UNITInstitute of Technology and Research – ITPUniversidade Estadual Paulista (Unesp)Cruz, Ana Carla F.G.Santos, Natália A.F.Jeraldo, Veronica de Lourdes S.Madi, Rubens R.Rosa, João Aristeu da [UNESP]Melo, Cláudia Moura de2020-12-12T02:44:24Z2020-12-12T02:44:24Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.actatropica.2020.105577Acta Tropica, v. 210.1873-62540001-706Xhttp://hdl.handle.net/11449/20188510.1016/j.actatropica.2020.1055772-s2.0-85086593716Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Tropicainfo:eu-repo/semantics/openAccess2024-06-24T13:08:13Zoai:repositorio.unesp.br:11449/201885Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:13:07.748593Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Shelter dogs as indicators for Trypanosoma cruzi infection in an urban area of Aracaju, Brazil |
title |
Shelter dogs as indicators for Trypanosoma cruzi infection in an urban area of Aracaju, Brazil |
spellingShingle |
Shelter dogs as indicators for Trypanosoma cruzi infection in an urban area of Aracaju, Brazil Cruz, Ana Carla F.G. Chagas Disease Disease reservoirs Indicators Trypanosoma cruzi |
title_short |
Shelter dogs as indicators for Trypanosoma cruzi infection in an urban area of Aracaju, Brazil |
title_full |
Shelter dogs as indicators for Trypanosoma cruzi infection in an urban area of Aracaju, Brazil |
title_fullStr |
Shelter dogs as indicators for Trypanosoma cruzi infection in an urban area of Aracaju, Brazil |
title_full_unstemmed |
Shelter dogs as indicators for Trypanosoma cruzi infection in an urban area of Aracaju, Brazil |
title_sort |
Shelter dogs as indicators for Trypanosoma cruzi infection in an urban area of Aracaju, Brazil |
author |
Cruz, Ana Carla F.G. |
author_facet |
Cruz, Ana Carla F.G. Santos, Natália A.F. Jeraldo, Veronica de Lourdes S. Madi, Rubens R. Rosa, João Aristeu da [UNESP] Melo, Cláudia Moura de |
author_role |
author |
author2 |
Santos, Natália A.F. Jeraldo, Veronica de Lourdes S. Madi, Rubens R. Rosa, João Aristeu da [UNESP] Melo, Cláudia Moura de |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Tiradentes University-UNIT Institute of Technology and Research – ITP Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cruz, Ana Carla F.G. Santos, Natália A.F. Jeraldo, Veronica de Lourdes S. Madi, Rubens R. Rosa, João Aristeu da [UNESP] Melo, Cláudia Moura de |
dc.subject.por.fl_str_mv |
Chagas Disease Disease reservoirs Indicators Trypanosoma cruzi |
topic |
Chagas Disease Disease reservoirs Indicators Trypanosoma cruzi |
description |
Shelters are places that receive certain species of domestic animals, mostly dogs and cats, that are normally abandoned. These animals are easy to handle because they are crowded and have limited movement. Dogs, due to the close relationship with humans, are considered the main domestic reservoirs in the peridomiciliary cycle of American trypanosomiasis infection in humans. Therefore, in several countries studies are carried out to assess the occurrence of infection in these animals. This work evaluated the occurrence of Trypanosoma cruzi in dogs from shelters in the city of Aracaju, Sergipe. This was an observational and cross-sectional study to detect the occurrence of T. cruzi in blood samples from shelter dogs using polymerase chain reaction (PCR) with the TcZ1/TcZ2 primers. A total of 168 blood samples from dogs (104 females and 64 males) were collected and subjected to DNA and PCR extraction, and seventeen (10.1%) samples showed amplification of the product of the expected size. The TcZ1/TcZ2 primer pair used is considered specific for T. cruzi, and it is capable of amplifying all strains without amplifying other Trypanosoma species. The shelters evaluated had different physical infrastructure; animals positive for T. cruzi were found in all of them, as confirmed by sequencing. It is concluded that shelter dogs, as well as other domestic animals, can function as indicators of the occurrence of T. cruzi in Aracaju-SE, providing relevant epidemiological information for health surveillance and monitoring of Trypanosomatid infections. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:44:24Z 2020-12-12T02:44:24Z 2020-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.actatropica.2020.105577 Acta Tropica, v. 210. 1873-6254 0001-706X http://hdl.handle.net/11449/201885 10.1016/j.actatropica.2020.105577 2-s2.0-85086593716 |
url |
http://dx.doi.org/10.1016/j.actatropica.2020.105577 http://hdl.handle.net/11449/201885 |
identifier_str_mv |
Acta Tropica, v. 210. 1873-6254 0001-706X 10.1016/j.actatropica.2020.105577 2-s2.0-85086593716 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Tropica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129299184615424 |