A practical fluorescence-based screening protocol for polyethylene terephthalate degrading microorganisms
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.21577/0103-5053.20170224 http://hdl.handle.net/11449/176253 |
Resumo: | We propose a practical, low-cost and selective fluorescence-based protocol adapted to identify polyethylene terephthalate (PET) degrading microorganisms. The microbial hydrolysis of PET nanoparticles was monitored by 2-hydroxyterephthalate, a fluorophore produced in situ after radical hydroxylation of terephthalic acid (TPA), the final hydrolysis product, by the Fenton reaction. Seven fungi presenting promising PET hydrolytic potential using the proposed microscale screening assay were identified. The strains evaluated presented a substantial increase of up to 18-fold in PET nanoparticles conversion, such as obtained by the fungus Trichoderma sp. C70, after their cultivation in a PET-enriched medium. The formation of other hydrolysis products, along with TPA, was observed using matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). |
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Repositório Institucional da UNESP |
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spelling |
A practical fluorescence-based screening protocol for polyethylene terephthalate degrading microorganismsBiocatalysisFenton reactionFluorescenceMicroorganism screeningPET degradationWe propose a practical, low-cost and selective fluorescence-based protocol adapted to identify polyethylene terephthalate (PET) degrading microorganisms. The microbial hydrolysis of PET nanoparticles was monitored by 2-hydroxyterephthalate, a fluorophore produced in situ after radical hydroxylation of terephthalic acid (TPA), the final hydrolysis product, by the Fenton reaction. Seven fungi presenting promising PET hydrolytic potential using the proposed microscale screening assay were identified. The strains evaluated presented a substantial increase of up to 18-fold in PET nanoparticles conversion, such as obtained by the fungus Trichoderma sp. C70, after their cultivation in a PET-enriched medium. The formation of other hydrolysis products, along with TPA, was observed using matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS).Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)National Research Council Sri LankaInstituto de Química Universidade Estadual de Campinas, PO Box 6154Centro de Estudos Ambientais Universidade Estadual Paulista (Unesp), Av. 24 A, 1515Divisão de Recursos Microbianos Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas (CPQBA) Universidade Estadual de Campinas, Rua Alexandre Cazellato, 999Departamento de Biotecnologia Centro R and D Petrobras, Av. Horácio Macedo, 950, Ilha do FundãoCentro de Estudos Ambientais Universidade Estadual Paulista (Unesp), Av. 24 A, 1515National Research Council Sri Lanka: 140741/2013-5National Research Council Sri Lanka: 2014/50249-8National Research Council Sri Lanka: 307885/2013-5Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)PetrobrasChaves, Michel R. B.Lima, Maria L. S. O.Malafatti‑Picca, Lusiane [UNESP]De Angelis, Derlene A.De Castro, Aline M.Valoni, ÉrikaMarsaioli, Anita J.2018-12-11T17:19:48Z2018-12-11T17:19:48Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1278-1285application/pdfhttp://dx.doi.org/10.21577/0103-5053.20170224Journal of the Brazilian Chemical Society, v. 29, n. 6, p. 1278-1285, 2018.1678-47900103-5053http://hdl.handle.net/11449/17625310.21577/0103-5053.20170224S0103-505320180006012782-s2.0-85046294073S0103-50532018000601278.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of the Brazilian Chemical Society0,3570,357info:eu-repo/semantics/openAccess2024-04-10T19:22:25Zoai:repositorio.unesp.br:11449/176253Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:45:28.345157Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A practical fluorescence-based screening protocol for polyethylene terephthalate degrading microorganisms |
title |
A practical fluorescence-based screening protocol for polyethylene terephthalate degrading microorganisms |
spellingShingle |
A practical fluorescence-based screening protocol for polyethylene terephthalate degrading microorganisms Chaves, Michel R. B. Biocatalysis Fenton reaction Fluorescence Microorganism screening PET degradation |
title_short |
A practical fluorescence-based screening protocol for polyethylene terephthalate degrading microorganisms |
title_full |
A practical fluorescence-based screening protocol for polyethylene terephthalate degrading microorganisms |
title_fullStr |
A practical fluorescence-based screening protocol for polyethylene terephthalate degrading microorganisms |
title_full_unstemmed |
A practical fluorescence-based screening protocol for polyethylene terephthalate degrading microorganisms |
title_sort |
A practical fluorescence-based screening protocol for polyethylene terephthalate degrading microorganisms |
author |
Chaves, Michel R. B. |
author_facet |
Chaves, Michel R. B. Lima, Maria L. S. O. Malafatti‑Picca, Lusiane [UNESP] De Angelis, Derlene A. De Castro, Aline M. Valoni, Érika Marsaioli, Anita J. |
author_role |
author |
author2 |
Lima, Maria L. S. O. Malafatti‑Picca, Lusiane [UNESP] De Angelis, Derlene A. De Castro, Aline M. Valoni, Érika Marsaioli, Anita J. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) Petrobras |
dc.contributor.author.fl_str_mv |
Chaves, Michel R. B. Lima, Maria L. S. O. Malafatti‑Picca, Lusiane [UNESP] De Angelis, Derlene A. De Castro, Aline M. Valoni, Érika Marsaioli, Anita J. |
dc.subject.por.fl_str_mv |
Biocatalysis Fenton reaction Fluorescence Microorganism screening PET degradation |
topic |
Biocatalysis Fenton reaction Fluorescence Microorganism screening PET degradation |
description |
We propose a practical, low-cost and selective fluorescence-based protocol adapted to identify polyethylene terephthalate (PET) degrading microorganisms. The microbial hydrolysis of PET nanoparticles was monitored by 2-hydroxyterephthalate, a fluorophore produced in situ after radical hydroxylation of terephthalic acid (TPA), the final hydrolysis product, by the Fenton reaction. Seven fungi presenting promising PET hydrolytic potential using the proposed microscale screening assay were identified. The strains evaluated presented a substantial increase of up to 18-fold in PET nanoparticles conversion, such as obtained by the fungus Trichoderma sp. C70, after their cultivation in a PET-enriched medium. The formation of other hydrolysis products, along with TPA, was observed using matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:19:48Z 2018-12-11T17:19:48Z 2018-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.21577/0103-5053.20170224 Journal of the Brazilian Chemical Society, v. 29, n. 6, p. 1278-1285, 2018. 1678-4790 0103-5053 http://hdl.handle.net/11449/176253 10.21577/0103-5053.20170224 S0103-50532018000601278 2-s2.0-85046294073 S0103-50532018000601278.pdf |
url |
http://dx.doi.org/10.21577/0103-5053.20170224 http://hdl.handle.net/11449/176253 |
identifier_str_mv |
Journal of the Brazilian Chemical Society, v. 29, n. 6, p. 1278-1285, 2018. 1678-4790 0103-5053 10.21577/0103-5053.20170224 S0103-50532018000601278 2-s2.0-85046294073 S0103-50532018000601278.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of the Brazilian Chemical Society 0,357 0,357 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1278-1285 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128413216538624 |