Shifting the balance: Heat stress challenges the symbiotic interactions of the Asian citrus psyllid, Diaphorina citri (Hemiptera, Liviidae)

Detalhes bibliográficos
Autor(a) principal: Dossi, Fabio Cleisto Alda
Data de Publicação: 2018
Outros Autores: da Silva, Edney Pereira [UNESP], Cônsoli, Fernando Luis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1086/699755
http://hdl.handle.net/11449/221220
Resumo: Global warming may impact biodiversity by disrupting biological interactions, including long-term insect-microbe mutualistic associations. Symbiont-mediated insect tolerance to high temperatures is an ecologically important trait that significantly influences an insect’s life history. Disruption of microbial symbionts that are required by insects would substantially impact their pest status. Diaphorina citri, a worldwide citrus pest, is associated with the mutualistic symbionts Candidatus Carsonella ruddii and Candidatus Profftella armatura. Wolbachia is also associated with D. citri, but its contribution to the host is unknown. Symbiont density is dependent on a range of factors, including the thermosensitivity of the host and/or symbiont to heat stress. Here, we predicted that short-term heat stress of D. citri would disrupt the host-symbiont phenological synchrony and differentially affect the growth and density of symbionts. We investigated the effects of exposing D. citri eggs to different temperatures for different periods of time on the growth dynamics of symbionts during the nymphal development of D. citri (first instar to fifth instar) by real-time polymerase chain reaction. Symbiont densities were assessed as the number of gene copies, using spe- cific molecular markers: 16S rRNA for Carsonella and Profftella and ftsZ for Wolbachia. Statistical modeling of the copy numbers of symbionts revealed differences in their growth patterns, particularly in the early instars of heat-shocked insects. Wolbachia was the only symbiont to benefit from heat-shock treatment. Although the symbionts responded differently to heat stress, the lack of differences in symbiont densities between treated and control late nymphs suggests the existence of an adaptive genetic process to restore phenological synchrony during the development of immatures in preparation for adult life. Our findings contribute tothe understanding of the potential deleterious effects of high temperatures on host-symbiont interactions. Our data also suggest that the effects of host exposure to high temperatures in symbiont growth are highly variable and dependent on the interactions among members of the community of symbionts harbored by a host. Such dependence points to unpredictable consequences for agroecosystems worldwide due to climate change-related effects on the ecological traits of symbiont-dependent insect pests.
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spelling Shifting the balance: Heat stress challenges the symbiotic interactions of the Asian citrus psyllid, Diaphorina citri (Hemiptera, Liviidae)Global warming may impact biodiversity by disrupting biological interactions, including long-term insect-microbe mutualistic associations. Symbiont-mediated insect tolerance to high temperatures is an ecologically important trait that significantly influences an insect’s life history. Disruption of microbial symbionts that are required by insects would substantially impact their pest status. Diaphorina citri, a worldwide citrus pest, is associated with the mutualistic symbionts Candidatus Carsonella ruddii and Candidatus Profftella armatura. Wolbachia is also associated with D. citri, but its contribution to the host is unknown. Symbiont density is dependent on a range of factors, including the thermosensitivity of the host and/or symbiont to heat stress. Here, we predicted that short-term heat stress of D. citri would disrupt the host-symbiont phenological synchrony and differentially affect the growth and density of symbionts. We investigated the effects of exposing D. citri eggs to different temperatures for different periods of time on the growth dynamics of symbionts during the nymphal development of D. citri (first instar to fifth instar) by real-time polymerase chain reaction. Symbiont densities were assessed as the number of gene copies, using spe- cific molecular markers: 16S rRNA for Carsonella and Profftella and ftsZ for Wolbachia. Statistical modeling of the copy numbers of symbionts revealed differences in their growth patterns, particularly in the early instars of heat-shocked insects. Wolbachia was the only symbiont to benefit from heat-shock treatment. Although the symbionts responded differently to heat stress, the lack of differences in symbiont densities between treated and control late nymphs suggests the existence of an adaptive genetic process to restore phenological synchrony during the development of immatures in preparation for adult life. Our findings contribute tothe understanding of the potential deleterious effects of high temperatures on host-symbiont interactions. Our data also suggest that the effects of host exposure to high temperatures in symbiont growth are highly variable and dependent on the interactions among members of the community of symbionts harbored by a host. Such dependence points to unpredictable consequences for agroecosystems worldwide due to climate change-related effects on the ecological traits of symbiont-dependent insect pests.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Insect Interactions Laboratory Department of Entomology and Acarology Luiz de Queiroz College of Agriculture University of São Paulo, Avenida Pádua Dias 11Avian Science Laboratory Department of Animal Science College of Agricultural and Veterinarian Sciences São Paulo State University, Via de Acesso Professor Paulo Donato Castellane, S/NAvian Science Laboratory Department of Animal Science College of Agricultural and Veterinarian Sciences São Paulo State University, Via de Acesso Professor Paulo Donato Castellane, S/NFAPESP: 2011/50877-0Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Dossi, Fabio Cleisto Aldada Silva, Edney Pereira [UNESP]Cônsoli, Fernando Luis2022-04-28T19:26:53Z2022-04-28T19:26:53Z2018-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article195-203http://dx.doi.org/10.1086/699755Biological Bulletin, v. 235, n. 3, p. 195-203, 2018.1939-86970006-3185http://hdl.handle.net/11449/22122010.1086/6997552-s2.0-85057769969Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiological Bulletininfo:eu-repo/semantics/openAccess2022-04-28T19:26:53Zoai:repositorio.unesp.br:11449/221220Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:16:17.890519Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Shifting the balance: Heat stress challenges the symbiotic interactions of the Asian citrus psyllid, Diaphorina citri (Hemiptera, Liviidae)
title Shifting the balance: Heat stress challenges the symbiotic interactions of the Asian citrus psyllid, Diaphorina citri (Hemiptera, Liviidae)
spellingShingle Shifting the balance: Heat stress challenges the symbiotic interactions of the Asian citrus psyllid, Diaphorina citri (Hemiptera, Liviidae)
Dossi, Fabio Cleisto Alda
title_short Shifting the balance: Heat stress challenges the symbiotic interactions of the Asian citrus psyllid, Diaphorina citri (Hemiptera, Liviidae)
title_full Shifting the balance: Heat stress challenges the symbiotic interactions of the Asian citrus psyllid, Diaphorina citri (Hemiptera, Liviidae)
title_fullStr Shifting the balance: Heat stress challenges the symbiotic interactions of the Asian citrus psyllid, Diaphorina citri (Hemiptera, Liviidae)
title_full_unstemmed Shifting the balance: Heat stress challenges the symbiotic interactions of the Asian citrus psyllid, Diaphorina citri (Hemiptera, Liviidae)
title_sort Shifting the balance: Heat stress challenges the symbiotic interactions of the Asian citrus psyllid, Diaphorina citri (Hemiptera, Liviidae)
author Dossi, Fabio Cleisto Alda
author_facet Dossi, Fabio Cleisto Alda
da Silva, Edney Pereira [UNESP]
Cônsoli, Fernando Luis
author_role author
author2 da Silva, Edney Pereira [UNESP]
Cônsoli, Fernando Luis
author2_role author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Dossi, Fabio Cleisto Alda
da Silva, Edney Pereira [UNESP]
Cônsoli, Fernando Luis
description Global warming may impact biodiversity by disrupting biological interactions, including long-term insect-microbe mutualistic associations. Symbiont-mediated insect tolerance to high temperatures is an ecologically important trait that significantly influences an insect’s life history. Disruption of microbial symbionts that are required by insects would substantially impact their pest status. Diaphorina citri, a worldwide citrus pest, is associated with the mutualistic symbionts Candidatus Carsonella ruddii and Candidatus Profftella armatura. Wolbachia is also associated with D. citri, but its contribution to the host is unknown. Symbiont density is dependent on a range of factors, including the thermosensitivity of the host and/or symbiont to heat stress. Here, we predicted that short-term heat stress of D. citri would disrupt the host-symbiont phenological synchrony and differentially affect the growth and density of symbionts. We investigated the effects of exposing D. citri eggs to different temperatures for different periods of time on the growth dynamics of symbionts during the nymphal development of D. citri (first instar to fifth instar) by real-time polymerase chain reaction. Symbiont densities were assessed as the number of gene copies, using spe- cific molecular markers: 16S rRNA for Carsonella and Profftella and ftsZ for Wolbachia. Statistical modeling of the copy numbers of symbionts revealed differences in their growth patterns, particularly in the early instars of heat-shocked insects. Wolbachia was the only symbiont to benefit from heat-shock treatment. Although the symbionts responded differently to heat stress, the lack of differences in symbiont densities between treated and control late nymphs suggests the existence of an adaptive genetic process to restore phenological synchrony during the development of immatures in preparation for adult life. Our findings contribute tothe understanding of the potential deleterious effects of high temperatures on host-symbiont interactions. Our data also suggest that the effects of host exposure to high temperatures in symbiont growth are highly variable and dependent on the interactions among members of the community of symbionts harbored by a host. Such dependence points to unpredictable consequences for agroecosystems worldwide due to climate change-related effects on the ecological traits of symbiont-dependent insect pests.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-01
2022-04-28T19:26:53Z
2022-04-28T19:26:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1086/699755
Biological Bulletin, v. 235, n. 3, p. 195-203, 2018.
1939-8697
0006-3185
http://hdl.handle.net/11449/221220
10.1086/699755
2-s2.0-85057769969
url http://dx.doi.org/10.1086/699755
http://hdl.handle.net/11449/221220
identifier_str_mv Biological Bulletin, v. 235, n. 3, p. 195-203, 2018.
1939-8697
0006-3185
10.1086/699755
2-s2.0-85057769969
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biological Bulletin
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 195-203
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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