Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array

• Main Author: Rigon, Roberta B. [UNESP]
• Publication Date: 2019
• Other Authors: Fachinetti, Naiara [UNESP], Severino, Patricia, Durazzo, Alessandra, Lucarini, Massimo, Atanasov, Atanas G., El Mamouni, Soukaina, Chorilli, Marlus [UNESP], Santini, Antonello, Souto, Eliana B.
• Format: Article
• Language: eng
• Source: Repositório Institucional da UNESP
• Download full: http://dx.doi.org/10.3390/app9224961; http://hdl.handle.net/11449/196407
• topic: trans-resveratrol; nanotechnology; solid lipid nanoparticles; high-performance liquid chromatography

A new method based on reverse-phase HPLC combined with photodiode array (PDA) was developed to quantify the release of trans-resveratrol (tRES) from solid lipid nanoparticles (SLN). The mobile phase was composed of 75:0:25 (V/V) water/methanol/acetonitrile at 0-3.5 min, 32.5:30.0:37.5 (V/V) water/methanol/acetonitrile at 3.6-5.8 min, and 75:0:25 (V/V) water/methanol/acetonitrile at 5.9-10 min. The flow rate was set at 1.0mL/min, and tRES was detected at the wavelength of 306.6 nm. A concentration range of 1-100 mu g/mL was used to obtain the linear calibration curve. SLN were produced by ultrasound technique to load 0.1% (wt/wt) of tRES, and the in vitro release of the drug was run in modified Franz diffusion cells. The mean recovery of tRES was found to be 96.84 +/- 0.32%. The intra-assay and inter-assay coefficients of variation were less than 5%. The proposed method was applied to in vitro permeability studies, and the Weibull model was found to be the one that best fits the tRES release, which is characterized by a simultaneous lipid chain relaxation and erosion during drug release.