Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array

Detalhes bibliográficos
Autor(a) principal: Rigon, Roberta B. [UNESP]
Data de Publicação: 2019
Outros Autores: Fachinetti, Naiara [UNESP], Severino, Patricia, Durazzo, Alessandra, Lucarini, Massimo, Atanasov, Atanas G., El Mamouni, Soukaina, Chorilli, Marlus [UNESP], Santini, Antonello, Souto, Eliana B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/app9224961
http://hdl.handle.net/11449/196407
Resumo: A new method based on reverse-phase HPLC combined with photodiode array (PDA) was developed to quantify the release of trans-resveratrol (tRES) from solid lipid nanoparticles (SLN). The mobile phase was composed of 75:0:25 (V/V) water/methanol/acetonitrile at 0-3.5 min, 32.5:30.0:37.5 (V/V) water/methanol/acetonitrile at 3.6-5.8 min, and 75:0:25 (V/V) water/methanol/acetonitrile at 5.9-10 min. The flow rate was set at 1.0mL/min, and tRES was detected at the wavelength of 306.6 nm. A concentration range of 1-100 mu g/mL was used to obtain the linear calibration curve. SLN were produced by ultrasound technique to load 0.1% (wt/wt) of tRES, and the in vitro release of the drug was run in modified Franz diffusion cells. The mean recovery of tRES was found to be 96.84 +/- 0.32%. The intra-assay and inter-assay coefficients of variation were less than 5%. The proposed method was applied to in vitro permeability studies, and the Weibull model was found to be the one that best fits the tRES release, which is characterized by a simultaneous lipid chain relaxation and erosion during drug release.
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spelling Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Arraytrans-resveratrolnanotechnologysolid lipid nanoparticleshigh-performance liquid chromatographyA new method based on reverse-phase HPLC combined with photodiode array (PDA) was developed to quantify the release of trans-resveratrol (tRES) from solid lipid nanoparticles (SLN). The mobile phase was composed of 75:0:25 (V/V) water/methanol/acetonitrile at 0-3.5 min, 32.5:30.0:37.5 (V/V) water/methanol/acetonitrile at 3.6-5.8 min, and 75:0:25 (V/V) water/methanol/acetonitrile at 5.9-10 min. The flow rate was set at 1.0mL/min, and tRES was detected at the wavelength of 306.6 nm. A concentration range of 1-100 mu g/mL was used to obtain the linear calibration curve. SLN were produced by ultrasound technique to load 0.1% (wt/wt) of tRES, and the in vitro release of the drug was run in modified Franz diffusion cells. The mean recovery of tRES was found to be 96.84 +/- 0.32%. The intra-assay and inter-assay coefficients of variation were less than 5%. The proposed method was applied to in vitro permeability studies, and the Weibull model was found to be the one that best fits the tRES release, which is characterized by a simultaneous lipid chain relaxation and erosion during drug release.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Portuguese Science and Technology Foundation (FCT/MCT)European Funds (PRODER/COMPETE)FEDERproject: Nutraceutica come supporto nutrizionale nel paziente oncologicoUniv Estadual Paulista, Sch Pharmaceut Sci Araraquara, Dept Drugs & Med, Rod Araraquara Jau,Km 1, BR-14801902 Araraquara, SP, BrazilUniv Tiradentes Unit, Tiradentes Inst, Av Murilo Dantas 300, BR-49010390 Aracaju, SE, BrazilCREA Res Ctr Food & Nutr, Via Ardeatina 546, I-00178 Rome, ItalyUniv Vienna, Dept Pharmacognosy, A-1090 Vienna, AustriaBulgarian Acad Sci, Inst Neurobiol, 23 Acad G Bonchev Str, Sofia 1113, BulgariaUniv Napoli Federico II, Dept Pharm, Via D Montesano 49, I-80131 Naples, ItalyUniv Coimbra FFUC, Fac Pharm, Dept Pharmaceut Technol, P-3000548 Coimbra, PortugalUniv Minho, CEB Ctr Biol Engn, Campus Gualtar, P-4710057 Braga, PortugalUniv Estadual Paulista, Sch Pharmaceut Sci Araraquara, Dept Drugs & Med, Rod Araraquara Jau,Km 1, BR-14801902 Araraquara, SP, BrazilEuropean Funds (PRODER/COMPETE): M-ERA-NET-0004/2015-PAIREDproject: Nutraceutica come supporto nutrizionale nel paziente oncologico: CUP: B83D18000140007MdpiUniversidade Estadual Paulista (Unesp)Univ Tiradentes UnitCREA Res Ctr Food & NutrUniv ViennaBulgarian Acad SciUniv Napoli Federico IIUniv Coimbra FFUCUniv MinhoRigon, Roberta B. [UNESP]Fachinetti, Naiara [UNESP]Severino, PatriciaDurazzo, AlessandraLucarini, MassimoAtanasov, Atanas G.El Mamouni, SoukainaChorilli, Marlus [UNESP]Santini, AntonelloSouto, Eliana B.2020-12-10T19:43:52Z2020-12-10T19:43:52Z2019-11-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15http://dx.doi.org/10.3390/app9224961Applied Sciences-basel. Basel: Mdpi, v. 9, n. 22, 15 p., 2019.http://hdl.handle.net/11449/19640710.3390/app9224961WOS:000502570800238Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied Sciences-baselinfo:eu-repo/semantics/openAccess2021-10-22T21:54:42Zoai:repositorio.unesp.br:11449/196407Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T21:54:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array
title Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array
spellingShingle Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array
Rigon, Roberta B. [UNESP]
trans-resveratrol
nanotechnology
solid lipid nanoparticles
high-performance liquid chromatography
title_short Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array
title_full Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array
title_fullStr Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array
title_full_unstemmed Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array
title_sort Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array
author Rigon, Roberta B. [UNESP]
author_facet Rigon, Roberta B. [UNESP]
Fachinetti, Naiara [UNESP]
Severino, Patricia
Durazzo, Alessandra
Lucarini, Massimo
Atanasov, Atanas G.
El Mamouni, Soukaina
Chorilli, Marlus [UNESP]
Santini, Antonello
Souto, Eliana B.
author_role author
author2 Fachinetti, Naiara [UNESP]
Severino, Patricia
Durazzo, Alessandra
Lucarini, Massimo
Atanasov, Atanas G.
El Mamouni, Soukaina
Chorilli, Marlus [UNESP]
Santini, Antonello
Souto, Eliana B.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ Tiradentes Unit
CREA Res Ctr Food & Nutr
Univ Vienna
Bulgarian Acad Sci
Univ Napoli Federico II
Univ Coimbra FFUC
Univ Minho
dc.contributor.author.fl_str_mv Rigon, Roberta B. [UNESP]
Fachinetti, Naiara [UNESP]
Severino, Patricia
Durazzo, Alessandra
Lucarini, Massimo
Atanasov, Atanas G.
El Mamouni, Soukaina
Chorilli, Marlus [UNESP]
Santini, Antonello
Souto, Eliana B.
dc.subject.por.fl_str_mv trans-resveratrol
nanotechnology
solid lipid nanoparticles
high-performance liquid chromatography
topic trans-resveratrol
nanotechnology
solid lipid nanoparticles
high-performance liquid chromatography
description A new method based on reverse-phase HPLC combined with photodiode array (PDA) was developed to quantify the release of trans-resveratrol (tRES) from solid lipid nanoparticles (SLN). The mobile phase was composed of 75:0:25 (V/V) water/methanol/acetonitrile at 0-3.5 min, 32.5:30.0:37.5 (V/V) water/methanol/acetonitrile at 3.6-5.8 min, and 75:0:25 (V/V) water/methanol/acetonitrile at 5.9-10 min. The flow rate was set at 1.0mL/min, and tRES was detected at the wavelength of 306.6 nm. A concentration range of 1-100 mu g/mL was used to obtain the linear calibration curve. SLN were produced by ultrasound technique to load 0.1% (wt/wt) of tRES, and the in vitro release of the drug was run in modified Franz diffusion cells. The mean recovery of tRES was found to be 96.84 +/- 0.32%. The intra-assay and inter-assay coefficients of variation were less than 5%. The proposed method was applied to in vitro permeability studies, and the Weibull model was found to be the one that best fits the tRES release, which is characterized by a simultaneous lipid chain relaxation and erosion during drug release.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-02
2020-12-10T19:43:52Z
2020-12-10T19:43:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/app9224961
Applied Sciences-basel. Basel: Mdpi, v. 9, n. 22, 15 p., 2019.
http://hdl.handle.net/11449/196407
10.3390/app9224961
WOS:000502570800238
url http://dx.doi.org/10.3390/app9224961
http://hdl.handle.net/11449/196407
identifier_str_mv Applied Sciences-basel. Basel: Mdpi, v. 9, n. 22, 15 p., 2019.
10.3390/app9224961
WOS:000502570800238
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Applied Sciences-basel
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15
dc.publisher.none.fl_str_mv Mdpi
publisher.none.fl_str_mv Mdpi
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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