Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/app9224961 http://hdl.handle.net/11449/196407 |
Resumo: | A new method based on reverse-phase HPLC combined with photodiode array (PDA) was developed to quantify the release of trans-resveratrol (tRES) from solid lipid nanoparticles (SLN). The mobile phase was composed of 75:0:25 (V/V) water/methanol/acetonitrile at 0-3.5 min, 32.5:30.0:37.5 (V/V) water/methanol/acetonitrile at 3.6-5.8 min, and 75:0:25 (V/V) water/methanol/acetonitrile at 5.9-10 min. The flow rate was set at 1.0mL/min, and tRES was detected at the wavelength of 306.6 nm. A concentration range of 1-100 mu g/mL was used to obtain the linear calibration curve. SLN were produced by ultrasound technique to load 0.1% (wt/wt) of tRES, and the in vitro release of the drug was run in modified Franz diffusion cells. The mean recovery of tRES was found to be 96.84 +/- 0.32%. The intra-assay and inter-assay coefficients of variation were less than 5%. The proposed method was applied to in vitro permeability studies, and the Weibull model was found to be the one that best fits the tRES release, which is characterized by a simultaneous lipid chain relaxation and erosion during drug release. |
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Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Arraytrans-resveratrolnanotechnologysolid lipid nanoparticleshigh-performance liquid chromatographyA new method based on reverse-phase HPLC combined with photodiode array (PDA) was developed to quantify the release of trans-resveratrol (tRES) from solid lipid nanoparticles (SLN). The mobile phase was composed of 75:0:25 (V/V) water/methanol/acetonitrile at 0-3.5 min, 32.5:30.0:37.5 (V/V) water/methanol/acetonitrile at 3.6-5.8 min, and 75:0:25 (V/V) water/methanol/acetonitrile at 5.9-10 min. The flow rate was set at 1.0mL/min, and tRES was detected at the wavelength of 306.6 nm. A concentration range of 1-100 mu g/mL was used to obtain the linear calibration curve. SLN were produced by ultrasound technique to load 0.1% (wt/wt) of tRES, and the in vitro release of the drug was run in modified Franz diffusion cells. The mean recovery of tRES was found to be 96.84 +/- 0.32%. The intra-assay and inter-assay coefficients of variation were less than 5%. The proposed method was applied to in vitro permeability studies, and the Weibull model was found to be the one that best fits the tRES release, which is characterized by a simultaneous lipid chain relaxation and erosion during drug release.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Portuguese Science and Technology Foundation (FCT/MCT)European Funds (PRODER/COMPETE)FEDERproject: Nutraceutica come supporto nutrizionale nel paziente oncologicoUniv Estadual Paulista, Sch Pharmaceut Sci Araraquara, Dept Drugs & Med, Rod Araraquara Jau,Km 1, BR-14801902 Araraquara, SP, BrazilUniv Tiradentes Unit, Tiradentes Inst, Av Murilo Dantas 300, BR-49010390 Aracaju, SE, BrazilCREA Res Ctr Food & Nutr, Via Ardeatina 546, I-00178 Rome, ItalyUniv Vienna, Dept Pharmacognosy, A-1090 Vienna, AustriaBulgarian Acad Sci, Inst Neurobiol, 23 Acad G Bonchev Str, Sofia 1113, BulgariaUniv Napoli Federico II, Dept Pharm, Via D Montesano 49, I-80131 Naples, ItalyUniv Coimbra FFUC, Fac Pharm, Dept Pharmaceut Technol, P-3000548 Coimbra, PortugalUniv Minho, CEB Ctr Biol Engn, Campus Gualtar, P-4710057 Braga, PortugalUniv Estadual Paulista, Sch Pharmaceut Sci Araraquara, Dept Drugs & Med, Rod Araraquara Jau,Km 1, BR-14801902 Araraquara, SP, BrazilEuropean Funds (PRODER/COMPETE): M-ERA-NET-0004/2015-PAIREDproject: Nutraceutica come supporto nutrizionale nel paziente oncologico: CUP: B83D18000140007MdpiUniversidade Estadual Paulista (Unesp)Univ Tiradentes UnitCREA Res Ctr Food & NutrUniv ViennaBulgarian Acad SciUniv Napoli Federico IIUniv Coimbra FFUCUniv MinhoRigon, Roberta B. [UNESP]Fachinetti, Naiara [UNESP]Severino, PatriciaDurazzo, AlessandraLucarini, MassimoAtanasov, Atanas G.El Mamouni, SoukainaChorilli, Marlus [UNESP]Santini, AntonelloSouto, Eliana B.2020-12-10T19:43:52Z2020-12-10T19:43:52Z2019-11-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15http://dx.doi.org/10.3390/app9224961Applied Sciences-basel. Basel: Mdpi, v. 9, n. 22, 15 p., 2019.http://hdl.handle.net/11449/19640710.3390/app9224961WOS:000502570800238Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied Sciences-baselinfo:eu-repo/semantics/openAccess2021-10-22T21:54:42Zoai:repositorio.unesp.br:11449/196407Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T21:54:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array |
title |
Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array |
spellingShingle |
Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array Rigon, Roberta B. [UNESP] trans-resveratrol nanotechnology solid lipid nanoparticles high-performance liquid chromatography |
title_short |
Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array |
title_full |
Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array |
title_fullStr |
Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array |
title_full_unstemmed |
Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array |
title_sort |
Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array |
author |
Rigon, Roberta B. [UNESP] |
author_facet |
Rigon, Roberta B. [UNESP] Fachinetti, Naiara [UNESP] Severino, Patricia Durazzo, Alessandra Lucarini, Massimo Atanasov, Atanas G. El Mamouni, Soukaina Chorilli, Marlus [UNESP] Santini, Antonello Souto, Eliana B. |
author_role |
author |
author2 |
Fachinetti, Naiara [UNESP] Severino, Patricia Durazzo, Alessandra Lucarini, Massimo Atanasov, Atanas G. El Mamouni, Soukaina Chorilli, Marlus [UNESP] Santini, Antonello Souto, Eliana B. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Tiradentes Unit CREA Res Ctr Food & Nutr Univ Vienna Bulgarian Acad Sci Univ Napoli Federico II Univ Coimbra FFUC Univ Minho |
dc.contributor.author.fl_str_mv |
Rigon, Roberta B. [UNESP] Fachinetti, Naiara [UNESP] Severino, Patricia Durazzo, Alessandra Lucarini, Massimo Atanasov, Atanas G. El Mamouni, Soukaina Chorilli, Marlus [UNESP] Santini, Antonello Souto, Eliana B. |
dc.subject.por.fl_str_mv |
trans-resveratrol nanotechnology solid lipid nanoparticles high-performance liquid chromatography |
topic |
trans-resveratrol nanotechnology solid lipid nanoparticles high-performance liquid chromatography |
description |
A new method based on reverse-phase HPLC combined with photodiode array (PDA) was developed to quantify the release of trans-resveratrol (tRES) from solid lipid nanoparticles (SLN). The mobile phase was composed of 75:0:25 (V/V) water/methanol/acetonitrile at 0-3.5 min, 32.5:30.0:37.5 (V/V) water/methanol/acetonitrile at 3.6-5.8 min, and 75:0:25 (V/V) water/methanol/acetonitrile at 5.9-10 min. The flow rate was set at 1.0mL/min, and tRES was detected at the wavelength of 306.6 nm. A concentration range of 1-100 mu g/mL was used to obtain the linear calibration curve. SLN were produced by ultrasound technique to load 0.1% (wt/wt) of tRES, and the in vitro release of the drug was run in modified Franz diffusion cells. The mean recovery of tRES was found to be 96.84 +/- 0.32%. The intra-assay and inter-assay coefficients of variation were less than 5%. The proposed method was applied to in vitro permeability studies, and the Weibull model was found to be the one that best fits the tRES release, which is characterized by a simultaneous lipid chain relaxation and erosion during drug release. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-02 2020-12-10T19:43:52Z 2020-12-10T19:43:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/app9224961 Applied Sciences-basel. Basel: Mdpi, v. 9, n. 22, 15 p., 2019. http://hdl.handle.net/11449/196407 10.3390/app9224961 WOS:000502570800238 |
url |
http://dx.doi.org/10.3390/app9224961 http://hdl.handle.net/11449/196407 |
identifier_str_mv |
Applied Sciences-basel. Basel: Mdpi, v. 9, n. 22, 15 p., 2019. 10.3390/app9224961 WOS:000502570800238 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Applied Sciences-basel |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
15 |
dc.publisher.none.fl_str_mv |
Mdpi |
publisher.none.fl_str_mv |
Mdpi |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965591911530496 |