Functionalized epigallocatechin gallate copolymer inhibit dentin matrices degradation: Mechanical, solubilized telopeptide and proteomic assays
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.dental.2018.08.797 http://hdl.handle.net/11449/184985 |
Resumo: | Objectives. We investigated the biostability of dentin organic matrices treated with epigallocatechin gallate (EGCG) in comparison to chlorhexidine (CHX), both extracted from functionalized copolymers. Methods. Copolymers were prepared with bis-GMA:TEGDMA and incorporated with 1% of EGCG or CHX (w/w). Blank copolymers were used as control. Copolymer samples were individually stored in 1 mL deionized water to produce copolymer extracts. Dentin matrices were obtained by demineralization of dentin disks in 10% phosphoric acid solution. Matrices were individually treated with 1 mL of the copolymer extracts or distilled water for 48 h. Collected extracts were analyzed by high-performance liquid chromatography (HPLC) for the presence and quantification of EGCG, CHX, and copolymer by-products. Treated dentin matrices were tested for ultimate tensile strength, gravimetric changes, and swelling ratio. The treatment media were tested for total protein concentration, and dentin protease activity through solubilized telopeptide (ICTP- and CTX-ELISA) assays. The treatment media were also submitted to proteomic analysis. Results. HPLC identified released unreacted copolymer species and showed higher release of CHX compared to EGCG from respective copolymer extracts. EGCG extract inhibited activity of dentin proteolytic enzymes and promoted collagen biomodification observed by the telopeptide assays and in the changes to dentin matrix properties. The proteomic results showed less collagenous peptide hits in the EGCG extract media compared to CHX, and suggest compound-specific dentin protein binding interactions. Significance. This study demonstrates specific antiproteolytic effect and protein interactions of EGCG copolymer extract directly on dentin. This represents an advancement in dental materials which can impact the clinical procedures. (C) 2018 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved. |
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Functionalized epigallocatechin gallate copolymer inhibit dentin matrices degradation: Mechanical, solubilized telopeptide and proteomic assaysEGCGProteomicsDentinBis-GMAHPLCTEGDMAObjectives. We investigated the biostability of dentin organic matrices treated with epigallocatechin gallate (EGCG) in comparison to chlorhexidine (CHX), both extracted from functionalized copolymers. Methods. Copolymers were prepared with bis-GMA:TEGDMA and incorporated with 1% of EGCG or CHX (w/w). Blank copolymers were used as control. Copolymer samples were individually stored in 1 mL deionized water to produce copolymer extracts. Dentin matrices were obtained by demineralization of dentin disks in 10% phosphoric acid solution. Matrices were individually treated with 1 mL of the copolymer extracts or distilled water for 48 h. Collected extracts were analyzed by high-performance liquid chromatography (HPLC) for the presence and quantification of EGCG, CHX, and copolymer by-products. Treated dentin matrices were tested for ultimate tensile strength, gravimetric changes, and swelling ratio. The treatment media were tested for total protein concentration, and dentin protease activity through solubilized telopeptide (ICTP- and CTX-ELISA) assays. The treatment media were also submitted to proteomic analysis. Results. HPLC identified released unreacted copolymer species and showed higher release of CHX compared to EGCG from respective copolymer extracts. EGCG extract inhibited activity of dentin proteolytic enzymes and promoted collagen biomodification observed by the telopeptide assays and in the changes to dentin matrix properties. The proteomic results showed less collagenous peptide hits in the EGCG extract media compared to CHX, and suggest compound-specific dentin protein binding interactions. Significance. This study demonstrates specific antiproteolytic effect and protein interactions of EGCG copolymer extract directly on dentin. This represents an advancement in dental materials which can impact the clinical procedures. (C) 2018 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.Dental Research Institute funds (University of Toronto, Faculty of Dentistry)NSERC (Natural Sciences and Engineering Research Council of Canada)Shanghai Jiaotong UniversitySaudi Arabian Cultural BureauUniv Toronto, Fac Dent, Dept Clin Sci Restorat, Toronto, ON, CanadaShanghai Jiao Tong Univ, Sch Med, Dept Prosthodont, Shanghai, Peoples R ChinaSao Paulo State Univ, Inst Sci & Technol Sao Jose dos Campos, Dept Restorat Dent, Sao Paulo, BrazilTaibah Univ, Coll Dent, Dept Restorat Dent Sci, Madina, Saudi ArabiaSao Paulo State Univ, Inst Sci & Technol Sao Jose dos Campos, Dept Restorat Dent, Sao Paulo, BrazilNSERC (Natural Sciences and Engineering Research Council of Canada): 2018-06489Elsevier B.V.Univ TorontoShanghai Jiao Tong UnivUniversidade Estadual Paulista (Unesp)Taibah UnivPrakki, AnuradhaXiong, YaoyangBortolatto, JanainaGoncalues, Lucelia Lemes [UNESP]Bafail, ArwaAnderson, GregStauroullalzis, Alexander Terry2019-10-04T12:31:46Z2019-10-04T12:31:46Z2018-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1625-1633http://dx.doi.org/10.1016/j.dental.2018.08.797Dental Materials. Oxford: Elsevier Sci Ltd, v. 34, n. 11, p. 1625-1633, 2018.0109-5641http://hdl.handle.net/11449/18498510.1016/j.dental.2018.08.797WOS:000448287400009Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDental Materialsinfo:eu-repo/semantics/openAccess2021-10-23T19:23:36Zoai:repositorio.unesp.br:11449/184985Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:49:38.239805Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Functionalized epigallocatechin gallate copolymer inhibit dentin matrices degradation: Mechanical, solubilized telopeptide and proteomic assays |
title |
Functionalized epigallocatechin gallate copolymer inhibit dentin matrices degradation: Mechanical, solubilized telopeptide and proteomic assays |
spellingShingle |
Functionalized epigallocatechin gallate copolymer inhibit dentin matrices degradation: Mechanical, solubilized telopeptide and proteomic assays Prakki, Anuradha EGCG Proteomics Dentin Bis-GMA HPLC TEGDMA |
title_short |
Functionalized epigallocatechin gallate copolymer inhibit dentin matrices degradation: Mechanical, solubilized telopeptide and proteomic assays |
title_full |
Functionalized epigallocatechin gallate copolymer inhibit dentin matrices degradation: Mechanical, solubilized telopeptide and proteomic assays |
title_fullStr |
Functionalized epigallocatechin gallate copolymer inhibit dentin matrices degradation: Mechanical, solubilized telopeptide and proteomic assays |
title_full_unstemmed |
Functionalized epigallocatechin gallate copolymer inhibit dentin matrices degradation: Mechanical, solubilized telopeptide and proteomic assays |
title_sort |
Functionalized epigallocatechin gallate copolymer inhibit dentin matrices degradation: Mechanical, solubilized telopeptide and proteomic assays |
author |
Prakki, Anuradha |
author_facet |
Prakki, Anuradha Xiong, Yaoyang Bortolatto, Janaina Goncalues, Lucelia Lemes [UNESP] Bafail, Arwa Anderson, Greg Stauroullalzis, Alexander Terry |
author_role |
author |
author2 |
Xiong, Yaoyang Bortolatto, Janaina Goncalues, Lucelia Lemes [UNESP] Bafail, Arwa Anderson, Greg Stauroullalzis, Alexander Terry |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Toronto Shanghai Jiao Tong Univ Universidade Estadual Paulista (Unesp) Taibah Univ |
dc.contributor.author.fl_str_mv |
Prakki, Anuradha Xiong, Yaoyang Bortolatto, Janaina Goncalues, Lucelia Lemes [UNESP] Bafail, Arwa Anderson, Greg Stauroullalzis, Alexander Terry |
dc.subject.por.fl_str_mv |
EGCG Proteomics Dentin Bis-GMA HPLC TEGDMA |
topic |
EGCG Proteomics Dentin Bis-GMA HPLC TEGDMA |
description |
Objectives. We investigated the biostability of dentin organic matrices treated with epigallocatechin gallate (EGCG) in comparison to chlorhexidine (CHX), both extracted from functionalized copolymers. Methods. Copolymers were prepared with bis-GMA:TEGDMA and incorporated with 1% of EGCG or CHX (w/w). Blank copolymers were used as control. Copolymer samples were individually stored in 1 mL deionized water to produce copolymer extracts. Dentin matrices were obtained by demineralization of dentin disks in 10% phosphoric acid solution. Matrices were individually treated with 1 mL of the copolymer extracts or distilled water for 48 h. Collected extracts were analyzed by high-performance liquid chromatography (HPLC) for the presence and quantification of EGCG, CHX, and copolymer by-products. Treated dentin matrices were tested for ultimate tensile strength, gravimetric changes, and swelling ratio. The treatment media were tested for total protein concentration, and dentin protease activity through solubilized telopeptide (ICTP- and CTX-ELISA) assays. The treatment media were also submitted to proteomic analysis. Results. HPLC identified released unreacted copolymer species and showed higher release of CHX compared to EGCG from respective copolymer extracts. EGCG extract inhibited activity of dentin proteolytic enzymes and promoted collagen biomodification observed by the telopeptide assays and in the changes to dentin matrix properties. The proteomic results showed less collagenous peptide hits in the EGCG extract media compared to CHX, and suggest compound-specific dentin protein binding interactions. Significance. This study demonstrates specific antiproteolytic effect and protein interactions of EGCG copolymer extract directly on dentin. This represents an advancement in dental materials which can impact the clinical procedures. (C) 2018 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-01 2019-10-04T12:31:46Z 2019-10-04T12:31:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.dental.2018.08.797 Dental Materials. Oxford: Elsevier Sci Ltd, v. 34, n. 11, p. 1625-1633, 2018. 0109-5641 http://hdl.handle.net/11449/184985 10.1016/j.dental.2018.08.797 WOS:000448287400009 |
url |
http://dx.doi.org/10.1016/j.dental.2018.08.797 http://hdl.handle.net/11449/184985 |
identifier_str_mv |
Dental Materials. Oxford: Elsevier Sci Ltd, v. 34, n. 11, p. 1625-1633, 2018. 0109-5641 10.1016/j.dental.2018.08.797 WOS:000448287400009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dental Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1625-1633 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128986479329280 |