Global proteomic analysis of the follicular fluid from brown brocket deer (Mazama gouazoubira; Fisher, 1814)

Detalhes bibliográficos
Autor(a) principal: Souza, Thais T. S.
Data de Publicação: 2022
Outros Autores: van Tilburg, Maurício F., Bezerra, Maria J. B., Rola, Luciana D., Pereira, Leda M. C., Duarte, José M. B. [UNESP], Chaves, Maiana S., Melo, Luciana M., Moura, Arlindo A. A. N., Freitas, Vicente J. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10344-022-01563-0
http://hdl.handle.net/11449/223493
Resumo: Understanding the protein composition of the follicular fluid from brown brocket deer would aid the development of a medium for in vitro embryo production, which would in turn contribute toward programs for species preservation. Proteomic shotgun is a sensitive tool for analysis of complex protein mixtures. Thus, this study aimed to use shotgun technique to investigate the proteome of fluid from small/medium and large follicles from brocket brown deer. The fluid was obtained by laparoscopy-guided follicular puncture from five females. Quantitative proteomic analysis was performed by multidimensional liquid chromatography coupled with mass spectrometry. A total of 226 proteins were identified in the follicular fluid, 53 and 60 of which were found only in small/medium and large follicles, respectively. One hundred and thirteen proteins were common to both groups of follicles. Quantitative analysis showed no significant differences (P > 0.05) in protein abundances in the follicular fluid from small/medium and large follicles. The proteins identified were classified by gene ontology terms, in silico interaction and assigned to 12 pathways indicated that proteins are involved in protein binding, catalytic activity, regulation of biological processes, extracellular matrix organization, and complement and coagulation cascades. In conclusion, these data add knowledge on the follicular development and provide original information on the follicular environment, which can contribute in the future to formulation of culture medium to use in embryo technology in brown brocket deer.
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spelling Global proteomic analysis of the follicular fluid from brown brocket deer (Mazama gouazoubira; Fisher, 1814)CervidFollicleProteinShotgunUnderstanding the protein composition of the follicular fluid from brown brocket deer would aid the development of a medium for in vitro embryo production, which would in turn contribute toward programs for species preservation. Proteomic shotgun is a sensitive tool for analysis of complex protein mixtures. Thus, this study aimed to use shotgun technique to investigate the proteome of fluid from small/medium and large follicles from brocket brown deer. The fluid was obtained by laparoscopy-guided follicular puncture from five females. Quantitative proteomic analysis was performed by multidimensional liquid chromatography coupled with mass spectrometry. A total of 226 proteins were identified in the follicular fluid, 53 and 60 of which were found only in small/medium and large follicles, respectively. One hundred and thirteen proteins were common to both groups of follicles. Quantitative analysis showed no significant differences (P > 0.05) in protein abundances in the follicular fluid from small/medium and large follicles. The proteins identified were classified by gene ontology terms, in silico interaction and assigned to 12 pathways indicated that proteins are involved in protein binding, catalytic activity, regulation of biological processes, extracellular matrix organization, and complement and coagulation cascades. In conclusion, these data add knowledge on the follicular development and provide original information on the follicular environment, which can contribute in the future to formulation of culture medium to use in embryo technology in brown brocket deer.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação Cearense de Apoio ao Desenvolvimento Científico e TecnológicoFaculty of Veterinary State University of CearáDepartment of Animal Science Federal Rural University of Semi-AridDepartment of Animal Science Federal University of CearáDepartment of Animal Science Federal University of ParaibaDepartment of Animal Science São Paulo State UniversityMolecular Genetics Research Unit University Center FametroDepartment of Animal Science São Paulo State UniversityCNPq: 461330/2014-8CAPES: 88881.142966/2017-01Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico: CI3-0093-0005580100/14State University of CearáFederal Rural University of Semi-AridFederal University of CearáFederal University of ParaibaUniversidade Estadual Paulista (UNESP)University Center FametroSouza, Thais T. S.van Tilburg, Maurício F.Bezerra, Maria J. B.Rola, Luciana D.Pereira, Leda M. C.Duarte, José M. B. [UNESP]Chaves, Maiana S.Melo, Luciana M.Moura, Arlindo A. A. N.Freitas, Vicente J. F.2022-04-28T19:50:56Z2022-04-28T19:50:56Z2022-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10344-022-01563-0European Journal of Wildlife Research, v. 68, n. 2, 2022.1439-05741612-4642http://hdl.handle.net/11449/22349310.1007/s10344-022-01563-02-s2.0-85124950078Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEuropean Journal of Wildlife Researchinfo:eu-repo/semantics/openAccess2022-04-28T19:50:56Zoai:repositorio.unesp.br:11449/223493Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:38:48.532909Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Global proteomic analysis of the follicular fluid from brown brocket deer (Mazama gouazoubira; Fisher, 1814)
title Global proteomic analysis of the follicular fluid from brown brocket deer (Mazama gouazoubira; Fisher, 1814)
spellingShingle Global proteomic analysis of the follicular fluid from brown brocket deer (Mazama gouazoubira; Fisher, 1814)
Souza, Thais T. S.
Cervid
Follicle
Protein
Shotgun
title_short Global proteomic analysis of the follicular fluid from brown brocket deer (Mazama gouazoubira; Fisher, 1814)
title_full Global proteomic analysis of the follicular fluid from brown brocket deer (Mazama gouazoubira; Fisher, 1814)
title_fullStr Global proteomic analysis of the follicular fluid from brown brocket deer (Mazama gouazoubira; Fisher, 1814)
title_full_unstemmed Global proteomic analysis of the follicular fluid from brown brocket deer (Mazama gouazoubira; Fisher, 1814)
title_sort Global proteomic analysis of the follicular fluid from brown brocket deer (Mazama gouazoubira; Fisher, 1814)
author Souza, Thais T. S.
author_facet Souza, Thais T. S.
van Tilburg, Maurício F.
Bezerra, Maria J. B.
Rola, Luciana D.
Pereira, Leda M. C.
Duarte, José M. B. [UNESP]
Chaves, Maiana S.
Melo, Luciana M.
Moura, Arlindo A. A. N.
Freitas, Vicente J. F.
author_role author
author2 van Tilburg, Maurício F.
Bezerra, Maria J. B.
Rola, Luciana D.
Pereira, Leda M. C.
Duarte, José M. B. [UNESP]
Chaves, Maiana S.
Melo, Luciana M.
Moura, Arlindo A. A. N.
Freitas, Vicente J. F.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv State University of Ceará
Federal Rural University of Semi-Arid
Federal University of Ceará
Federal University of Paraiba
Universidade Estadual Paulista (UNESP)
University Center Fametro
dc.contributor.author.fl_str_mv Souza, Thais T. S.
van Tilburg, Maurício F.
Bezerra, Maria J. B.
Rola, Luciana D.
Pereira, Leda M. C.
Duarte, José M. B. [UNESP]
Chaves, Maiana S.
Melo, Luciana M.
Moura, Arlindo A. A. N.
Freitas, Vicente J. F.
dc.subject.por.fl_str_mv Cervid
Follicle
Protein
Shotgun
topic Cervid
Follicle
Protein
Shotgun
description Understanding the protein composition of the follicular fluid from brown brocket deer would aid the development of a medium for in vitro embryo production, which would in turn contribute toward programs for species preservation. Proteomic shotgun is a sensitive tool for analysis of complex protein mixtures. Thus, this study aimed to use shotgun technique to investigate the proteome of fluid from small/medium and large follicles from brocket brown deer. The fluid was obtained by laparoscopy-guided follicular puncture from five females. Quantitative proteomic analysis was performed by multidimensional liquid chromatography coupled with mass spectrometry. A total of 226 proteins were identified in the follicular fluid, 53 and 60 of which were found only in small/medium and large follicles, respectively. One hundred and thirteen proteins were common to both groups of follicles. Quantitative analysis showed no significant differences (P > 0.05) in protein abundances in the follicular fluid from small/medium and large follicles. The proteins identified were classified by gene ontology terms, in silico interaction and assigned to 12 pathways indicated that proteins are involved in protein binding, catalytic activity, regulation of biological processes, extracellular matrix organization, and complement and coagulation cascades. In conclusion, these data add knowledge on the follicular development and provide original information on the follicular environment, which can contribute in the future to formulation of culture medium to use in embryo technology in brown brocket deer.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-28T19:50:56Z
2022-04-28T19:50:56Z
2022-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10344-022-01563-0
European Journal of Wildlife Research, v. 68, n. 2, 2022.
1439-0574
1612-4642
http://hdl.handle.net/11449/223493
10.1007/s10344-022-01563-0
2-s2.0-85124950078
url http://dx.doi.org/10.1007/s10344-022-01563-0
http://hdl.handle.net/11449/223493
identifier_str_mv European Journal of Wildlife Research, v. 68, n. 2, 2022.
1439-0574
1612-4642
10.1007/s10344-022-01563-0
2-s2.0-85124950078
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv European Journal of Wildlife Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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