Molecular underpinnings of the early brain developmental response to differential feeding in the honey bee Apis mellifera
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bbagrm.2021.194732 http://hdl.handle.net/11449/222058 |
Resumo: | Brain differential morphogenesis in females is one of the major phenotypic manifestations of caste development in honey bees. Brain diphenism appears at the fourth larval phase as a result of the differential feeding regime developing females are submitted during early phases of larval development. Here, we used a forward genetics approach to test the early brain molecular response to differential feeding leading to the brain diphenism observed at later developmental phases. Using RNA sequencing analysis, we identified 53 differentially expressed genes (DEGs) between the brains of queens and workers at the third larval phase. Since miRNAs have been suggested to play a role in caste differentiation after horizontal and vertical transmission, we tested their potential participation in regulating the DEGs. The miRNA-mRNA interaction network, including the DEGs and the royal- and worker-jelly enriched miRNA populations, revealed a subset of miRNAs potentially involved in regulating the expression of DEGs. The interaction of miR-34, miR-210, and miR-317 with Takeout, Neurotrophin-1, Forked, and Masquerade genes was experimentally confirmed using a luciferase reporter system. Taken together, our results reconstruct the regulatory network that governs the development of the early brain diphenism in honey bees. |
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Molecular underpinnings of the early brain developmental response to differential feeding in the honey bee Apis melliferaDevelopmentEarly brainGene expressionHoneybeeMicroRNANutritionBrain differential morphogenesis in females is one of the major phenotypic manifestations of caste development in honey bees. Brain diphenism appears at the fourth larval phase as a result of the differential feeding regime developing females are submitted during early phases of larval development. Here, we used a forward genetics approach to test the early brain molecular response to differential feeding leading to the brain diphenism observed at later developmental phases. Using RNA sequencing analysis, we identified 53 differentially expressed genes (DEGs) between the brains of queens and workers at the third larval phase. Since miRNAs have been suggested to play a role in caste differentiation after horizontal and vertical transmission, we tested their potential participation in regulating the DEGs. The miRNA-mRNA interaction network, including the DEGs and the royal- and worker-jelly enriched miRNA populations, revealed a subset of miRNAs potentially involved in regulating the expression of DEGs. The interaction of miR-34, miR-210, and miR-317 with Takeout, Neurotrophin-1, Forked, and Masquerade genes was experimentally confirmed using a luciferase reporter system. Taken together, our results reconstruct the regulatory network that governs the development of the early brain diphenism in honey bees.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Financiadora de Estudos e ProjetosFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Departamento de Biologia Celular e do Desenvolvimento Instituto de Ciências Biomédicas Universidade Federal de Alfenas UNIFAL-MGDepartamento de Genética Faculdade de Medicina de Ribeirão Preto Universidade de São PauloGriffith Institute for Drug Discovery Griffith UniversityFaculdade de Ciências Agrárias e Veterinárias UNESP - Universidade Estadual PaulistaDepartamento de Biologia Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São PauloFaculdade de Ciências Agrárias e Veterinárias UNESP - Universidade Estadual PaulistaFAPESP: 2005/03926-5FAPESP: 2011/03171-5CNPq: 406734/2018-6FAPEMIG: APQ-02134-14UNIFAL-MGUniversidade de São Paulo (USP)Griffith UniversityUniversidade Estadual Paulista (UNESP)Vieira, Joseanade Paula Freitas, Flávia CristinaSantos Cristino, AlexandreGuariz Pinheiro, Daniel [UNESP]Aguiar, Luiz RobertoFramartino Bezerra Laure, Marcela AparecidaRosatto Moda, Lívia MariaPaulino Simões, Zilá LuzBarchuk, Angel Roberto2022-04-28T19:42:08Z2022-04-28T19:42:08Z2021-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bbagrm.2021.194732Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, v. 1864, n. 9, 2021.1876-43201874-9399http://hdl.handle.net/11449/22205810.1016/j.bbagrm.2021.1947322-s2.0-85111282761Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiochimica et Biophysica Acta - Gene Regulatory Mechanismsinfo:eu-repo/semantics/openAccess2022-04-28T19:42:09Zoai:repositorio.unesp.br:11449/222058Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:58:11.983512Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular underpinnings of the early brain developmental response to differential feeding in the honey bee Apis mellifera |
title |
Molecular underpinnings of the early brain developmental response to differential feeding in the honey bee Apis mellifera |
spellingShingle |
Molecular underpinnings of the early brain developmental response to differential feeding in the honey bee Apis mellifera Vieira, Joseana Development Early brain Gene expression Honeybee MicroRNA Nutrition |
title_short |
Molecular underpinnings of the early brain developmental response to differential feeding in the honey bee Apis mellifera |
title_full |
Molecular underpinnings of the early brain developmental response to differential feeding in the honey bee Apis mellifera |
title_fullStr |
Molecular underpinnings of the early brain developmental response to differential feeding in the honey bee Apis mellifera |
title_full_unstemmed |
Molecular underpinnings of the early brain developmental response to differential feeding in the honey bee Apis mellifera |
title_sort |
Molecular underpinnings of the early brain developmental response to differential feeding in the honey bee Apis mellifera |
author |
Vieira, Joseana |
author_facet |
Vieira, Joseana de Paula Freitas, Flávia Cristina Santos Cristino, Alexandre Guariz Pinheiro, Daniel [UNESP] Aguiar, Luiz Roberto Framartino Bezerra Laure, Marcela Aparecida Rosatto Moda, Lívia Maria Paulino Simões, Zilá Luz Barchuk, Angel Roberto |
author_role |
author |
author2 |
de Paula Freitas, Flávia Cristina Santos Cristino, Alexandre Guariz Pinheiro, Daniel [UNESP] Aguiar, Luiz Roberto Framartino Bezerra Laure, Marcela Aparecida Rosatto Moda, Lívia Maria Paulino Simões, Zilá Luz Barchuk, Angel Roberto |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
UNIFAL-MG Universidade de São Paulo (USP) Griffith University Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Vieira, Joseana de Paula Freitas, Flávia Cristina Santos Cristino, Alexandre Guariz Pinheiro, Daniel [UNESP] Aguiar, Luiz Roberto Framartino Bezerra Laure, Marcela Aparecida Rosatto Moda, Lívia Maria Paulino Simões, Zilá Luz Barchuk, Angel Roberto |
dc.subject.por.fl_str_mv |
Development Early brain Gene expression Honeybee MicroRNA Nutrition |
topic |
Development Early brain Gene expression Honeybee MicroRNA Nutrition |
description |
Brain differential morphogenesis in females is one of the major phenotypic manifestations of caste development in honey bees. Brain diphenism appears at the fourth larval phase as a result of the differential feeding regime developing females are submitted during early phases of larval development. Here, we used a forward genetics approach to test the early brain molecular response to differential feeding leading to the brain diphenism observed at later developmental phases. Using RNA sequencing analysis, we identified 53 differentially expressed genes (DEGs) between the brains of queens and workers at the third larval phase. Since miRNAs have been suggested to play a role in caste differentiation after horizontal and vertical transmission, we tested their potential participation in regulating the DEGs. The miRNA-mRNA interaction network, including the DEGs and the royal- and worker-jelly enriched miRNA populations, revealed a subset of miRNAs potentially involved in regulating the expression of DEGs. The interaction of miR-34, miR-210, and miR-317 with Takeout, Neurotrophin-1, Forked, and Masquerade genes was experimentally confirmed using a luciferase reporter system. Taken together, our results reconstruct the regulatory network that governs the development of the early brain diphenism in honey bees. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-01 2022-04-28T19:42:08Z 2022-04-28T19:42:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bbagrm.2021.194732 Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, v. 1864, n. 9, 2021. 1876-4320 1874-9399 http://hdl.handle.net/11449/222058 10.1016/j.bbagrm.2021.194732 2-s2.0-85111282761 |
url |
http://dx.doi.org/10.1016/j.bbagrm.2021.194732 http://hdl.handle.net/11449/222058 |
identifier_str_mv |
Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, v. 1864, n. 9, 2021. 1876-4320 1874-9399 10.1016/j.bbagrm.2021.194732 2-s2.0-85111282761 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochimica et Biophysica Acta - Gene Regulatory Mechanisms |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128587894620160 |