Characterization of &ITvasa&IT homolog in a neotropical catfish, Jundia (&ITRhamdia quelen&IT): Molecular cloning and expression analysis during embryonic and larval development
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.gene.2018.02.029 http://hdl.handle.net/11449/164076 |
Resumo: | We have characterized the full-length vasa cDNA from Jundia, Rhamdia quelen (Heptapteridae, Siluriformes). vasa encodes a member of the DEAD-box protein family of ATP-dependent RNA helicases. This protein is highly conserved among different organisms and its role is associated with RNA metabolism. In the majority of the investigated species, vasa is restricted to the germ cell lineage and its expression has been used to study germline development in many organisms, including fish. The deduced R. quelen vasa amino acid sequence displayed high similarity with Vasa protein sequences from other organisms, and did not cluster with PL10 or P68 DEAD-box protein subfamilies. We also reported that there is no other isoform for vasa mRNA in R. quelen gonads. Expression analysis by RT-PCR and qPCR showed vasa transcripts exclusively expressed in the germ cells of R. quelen gonads. R. quelen vasa mRNA was maternally inherited, and was detected in the migrating primordial germ cells (PGCs) until 264 h post-fertilization during embryonic and larval development. This work has characterized for the first time the full-length R. quelen vasa cDNA, and describes its expression patterns during R. quelen embryonic and larval development. Our results will contribute to the basic reproductive biology of this native species, and will support studies using vasa as a germ cell marker in different biotechnological studies, such as germ cell transplantation. |
id |
UNSP_106117ad6d7735ddb6182b5836ea3410 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/164076 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Characterization of &ITvasa&IT homolog in a neotropical catfish, Jundia (&ITRhamdia quelen&IT): Molecular cloning and expression analysis during embryonic and larval developmentvasaTeleostPrimordial germ cellGerm cellWe have characterized the full-length vasa cDNA from Jundia, Rhamdia quelen (Heptapteridae, Siluriformes). vasa encodes a member of the DEAD-box protein family of ATP-dependent RNA helicases. This protein is highly conserved among different organisms and its role is associated with RNA metabolism. In the majority of the investigated species, vasa is restricted to the germ cell lineage and its expression has been used to study germline development in many organisms, including fish. The deduced R. quelen vasa amino acid sequence displayed high similarity with Vasa protein sequences from other organisms, and did not cluster with PL10 or P68 DEAD-box protein subfamilies. We also reported that there is no other isoform for vasa mRNA in R. quelen gonads. Expression analysis by RT-PCR and qPCR showed vasa transcripts exclusively expressed in the germ cells of R. quelen gonads. R. quelen vasa mRNA was maternally inherited, and was detected in the migrating primordial germ cells (PGCs) until 264 h post-fertilization during embryonic and larval development. This work has characterized for the first time the full-length R. quelen vasa cDNA, and describes its expression patterns during R. quelen embryonic and larval development. Our results will contribute to the basic reproductive biology of this native species, and will support studies using vasa as a germ cell marker in different biotechnological studies, such as germ cell transplantation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Norwegian Research Council BIOTEK2021 project SALMOSTERILESao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, Reprod & Mol Biol Grp, Botucatu, SP, BrazilUniv Estadual Oeste Parana, Ctr Engn & Exact Sci, Rua Fac 645, BR-85903000 Toledo, PR, BrazilUniv Utrecht, Div Dev Biol, Dept Biol, Reprod Biol Grp,Fac Sci, Hugo R Kruyt Bldg,Padualaan 8, NL-3584 CH Utrecht, NetherlandsSao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, Reprod & Mol Biol Grp, Botucatu, SP, BrazilFAPESP: 14/07620-7FAPESP: 12/00423-6Norwegian Research Council BIOTEK2021 project SALMOSTERILE: 221648Elsevier B.V.Universidade Estadual Paulista (Unesp)Univ Estadual Oeste ParanaUniv UtrechtRicci, Juliana M. B. [UNESP]Martinez, Emanuel R. M. [UNESP]Butzge, Arno J. [UNESP]Doretto, Lucas B. [UNESP]Oliveira, Marcos A. [UNESP]Bombardelli, Robie AllanBogerd, JanNobrega, Rafael H. [UNESP]2018-11-26T17:49:00Z2018-11-26T17:49:00Z2018-05-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article116-126application/pdfhttp://dx.doi.org/10.1016/j.gene.2018.02.029Gene. Amsterdam: Elsevier Science Bv, v. 654, p. 116-126, 2018.0378-1119http://hdl.handle.net/11449/16407610.1016/j.gene.2018.02.029WOS:000429393900016WOS000429393900016.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGene1,019info:eu-repo/semantics/openAccess2023-12-09T06:22:20Zoai:repositorio.unesp.br:11449/164076Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-09T06:22:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of &ITvasa&IT homolog in a neotropical catfish, Jundia (&ITRhamdia quelen&IT): Molecular cloning and expression analysis during embryonic and larval development |
title |
Characterization of &ITvasa&IT homolog in a neotropical catfish, Jundia (&ITRhamdia quelen&IT): Molecular cloning and expression analysis during embryonic and larval development |
spellingShingle |
Characterization of &ITvasa&IT homolog in a neotropical catfish, Jundia (&ITRhamdia quelen&IT): Molecular cloning and expression analysis during embryonic and larval development Ricci, Juliana M. B. [UNESP] vasa Teleost Primordial germ cell Germ cell |
title_short |
Characterization of &ITvasa&IT homolog in a neotropical catfish, Jundia (&ITRhamdia quelen&IT): Molecular cloning and expression analysis during embryonic and larval development |
title_full |
Characterization of &ITvasa&IT homolog in a neotropical catfish, Jundia (&ITRhamdia quelen&IT): Molecular cloning and expression analysis during embryonic and larval development |
title_fullStr |
Characterization of &ITvasa&IT homolog in a neotropical catfish, Jundia (&ITRhamdia quelen&IT): Molecular cloning and expression analysis during embryonic and larval development |
title_full_unstemmed |
Characterization of &ITvasa&IT homolog in a neotropical catfish, Jundia (&ITRhamdia quelen&IT): Molecular cloning and expression analysis during embryonic and larval development |
title_sort |
Characterization of &ITvasa&IT homolog in a neotropical catfish, Jundia (&ITRhamdia quelen&IT): Molecular cloning and expression analysis during embryonic and larval development |
author |
Ricci, Juliana M. B. [UNESP] |
author_facet |
Ricci, Juliana M. B. [UNESP] Martinez, Emanuel R. M. [UNESP] Butzge, Arno J. [UNESP] Doretto, Lucas B. [UNESP] Oliveira, Marcos A. [UNESP] Bombardelli, Robie Allan Bogerd, Jan Nobrega, Rafael H. [UNESP] |
author_role |
author |
author2 |
Martinez, Emanuel R. M. [UNESP] Butzge, Arno J. [UNESP] Doretto, Lucas B. [UNESP] Oliveira, Marcos A. [UNESP] Bombardelli, Robie Allan Bogerd, Jan Nobrega, Rafael H. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Estadual Oeste Parana Univ Utrecht |
dc.contributor.author.fl_str_mv |
Ricci, Juliana M. B. [UNESP] Martinez, Emanuel R. M. [UNESP] Butzge, Arno J. [UNESP] Doretto, Lucas B. [UNESP] Oliveira, Marcos A. [UNESP] Bombardelli, Robie Allan Bogerd, Jan Nobrega, Rafael H. [UNESP] |
dc.subject.por.fl_str_mv |
vasa Teleost Primordial germ cell Germ cell |
topic |
vasa Teleost Primordial germ cell Germ cell |
description |
We have characterized the full-length vasa cDNA from Jundia, Rhamdia quelen (Heptapteridae, Siluriformes). vasa encodes a member of the DEAD-box protein family of ATP-dependent RNA helicases. This protein is highly conserved among different organisms and its role is associated with RNA metabolism. In the majority of the investigated species, vasa is restricted to the germ cell lineage and its expression has been used to study germline development in many organisms, including fish. The deduced R. quelen vasa amino acid sequence displayed high similarity with Vasa protein sequences from other organisms, and did not cluster with PL10 or P68 DEAD-box protein subfamilies. We also reported that there is no other isoform for vasa mRNA in R. quelen gonads. Expression analysis by RT-PCR and qPCR showed vasa transcripts exclusively expressed in the germ cells of R. quelen gonads. R. quelen vasa mRNA was maternally inherited, and was detected in the migrating primordial germ cells (PGCs) until 264 h post-fertilization during embryonic and larval development. This work has characterized for the first time the full-length R. quelen vasa cDNA, and describes its expression patterns during R. quelen embryonic and larval development. Our results will contribute to the basic reproductive biology of this native species, and will support studies using vasa as a germ cell marker in different biotechnological studies, such as germ cell transplantation. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:49:00Z 2018-11-26T17:49:00Z 2018-05-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.gene.2018.02.029 Gene. Amsterdam: Elsevier Science Bv, v. 654, p. 116-126, 2018. 0378-1119 http://hdl.handle.net/11449/164076 10.1016/j.gene.2018.02.029 WOS:000429393900016 WOS000429393900016.pdf |
url |
http://dx.doi.org/10.1016/j.gene.2018.02.029 http://hdl.handle.net/11449/164076 |
identifier_str_mv |
Gene. Amsterdam: Elsevier Science Bv, v. 654, p. 116-126, 2018. 0378-1119 10.1016/j.gene.2018.02.029 WOS:000429393900016 WOS000429393900016.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gene 1,019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
116-126 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965226932633600 |