Influência do ciclo estral no diâmetro oocitário em cadelas

• Autor(a) principal: Pereira, Leda Maria Costa [UNESP]
• Data de Publicação: 2014
• Outros Autores: Bersano, Paulo Ricardo Oliveira [UNESP], Lopes, Maria Denise [UNESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://www.fmvz.unesp.br/rvz/index.php/rvz/article/view/508; http://hdl.handle.net/11449/141271
• topic: Oocyte; Bitches; Diameter; Estrous cycle; Ovocito; Perras; Diámetro; Ciclo estral; Oócito; Cadelas; Diâmetro; Ciclo estral

The low meiotic competence of canine oocytes when subjected to conditions of artificial cultive is considered a major obstacle to the development of reproductive biotechnologies in this species. Low rates of IVM may be linked to the size of oocytes recovered from different phases of the estrous cycle. The objective of this study is to evaluate the relationship between the phases of the estrous cycle - luteal phase and anestrus, with oocyte diameter of bitches. A total of 41 female dogs, aged 6 months to 7 years, subject to ovariosalpingohisterectomy elective in the Clinic of Small Animal Reproduction, Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, UNESP, Botucatu. Ovaries were sliced and selected only those COCs grade I, being subjected to the solution of 2% hyaluronidase to remove cumulus cells. After this process, the oocytes were stained with 10 / ml of Hoechst 33342 for assessment of oocyte diameter without the zona pellucida. The results showed no statistically significant difference between the oocytes from anestrous and luteal phases (77.62 µm x 78.64 µm ). Thus, there is a need for further studies to be able to have a proper understanding of the factors that may be interfering with oocyte competence and should not be considered only a determining factor since the coordinated action of several factors contributing to the growth and developing oocyte.