Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga

Detalhes bibliográficos
Autor(a) principal: Chimetto Tonon, Luciane A.
Data de Publicação: 2021
Outros Autores: Rua, Cintia, Crnkovic, Camila M., Bernardi, Darlon I., Pires Junior, Osmindo R., Haddad, Célio F.B. [UNESP], Pedrosa, Carolinada S.G., Souza, Leticia R.Q., Rehen, Stevens K., de Azevedo, Gustavo P.R., Thompson, Cristiane C., Thompson, Fabiano L., Berlinck, Roberto G.S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.toxicon.2021.10.002
http://hdl.handle.net/11449/229743
Resumo: The genus Brachycephalus includes small species of aposematic anurans known as microendemic, occurring in the mountains of the Atlantic Forest. Brachycephalus ephippium, B. nodoterga and B. pernix have been reported to contain the neurotoxin tetrodotoxin in skin and viscera. The biological conservation of several Brachycephalus species is currently threatened by climate change, deforestation, and the pandemic caused by the fungus Batrachochytrium dendrobatidis (Bd). Despite the well-known importance of amphibians’ associated bacteria in the defensive role against pathogens, there is still a poor understanding of amphibian microbiome composition. The present study investigated the composition of B. pitanga microbial community and the presence of TTX in the host and in cultures of bacterial isolates, using a combination of metagenomics, bacterial culture isolation, mass spectrometry and metabolomic analyses. Results of culture-dependent and -independent analyses characterized the microbial communities associated with the skin and viscera of B. pitanga. Mass spectrometry analysis indicated the presence of TTX in host tissues, while bacterial production of TTX was not observed under the experimental conditions used in this investigation. This is the first report confirming the occurrence of TTX in B. pitanga.
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spelling Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitangaBrachycephalidaeMetabolomicsMetagenomicsMicrobiotaToxinThe genus Brachycephalus includes small species of aposematic anurans known as microendemic, occurring in the mountains of the Atlantic Forest. Brachycephalus ephippium, B. nodoterga and B. pernix have been reported to contain the neurotoxin tetrodotoxin in skin and viscera. The biological conservation of several Brachycephalus species is currently threatened by climate change, deforestation, and the pandemic caused by the fungus Batrachochytrium dendrobatidis (Bd). Despite the well-known importance of amphibians’ associated bacteria in the defensive role against pathogens, there is still a poor understanding of amphibian microbiome composition. The present study investigated the composition of B. pitanga microbial community and the presence of TTX in the host and in cultures of bacterial isolates, using a combination of metagenomics, bacterial culture isolation, mass spectrometry and metabolomic analyses. Results of culture-dependent and -independent analyses characterized the microbial communities associated with the skin and viscera of B. pitanga. Mass spectrometry analysis indicated the presence of TTX in host tissues, while bacterial production of TTX was not observed under the experimental conditions used in this investigation. This is the first report confirming the occurrence of TTX in B. pitanga.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Instituto de Química de São Carlos Universidade de São Paulo, CP 780Instituto de Biologia SAGE-COPPE Universidade Federal do Rio de Janeiro (UFRJ)Departamento de Tecnologia Bioquímico-Farmacêutica (FBT) Faculdade de Ciências Farmacêuticas Universidade de São PauloLaboratório de Toxinologia Instituto de Biologia Universidade de BrasíliaDepartamento de Biodiversidade e Centro de Aquicultura Instituto de Biociências Universidade Estadual Paulista (UNESP)Instituto D'Or de Pesquisa e Ensino (IDOR)Instituto de Ciências Biomédicas Universidade Federal do Rio de Janeiro (UFRJ)Departamento de Biodiversidade e Centro de Aquicultura Instituto de Biociências Universidade Estadual Paulista (UNESP)FAPESP: 2013/50228-8FAPESP: 2013/50741-7FAPESP: 2016/14375-4FAPESP: 2016/21341-9FAPESP: 2019/00447-1FAPERJ: 202.488/2019Universidade de São Paulo (USP)Universidade Federal do Rio de Janeiro (UFRJ)Universidade de Brasília (UnB)Universidade Estadual Paulista (UNESP)Instituto D'Or de Pesquisa e Ensino (IDOR)Chimetto Tonon, Luciane A.Rua, CintiaCrnkovic, Camila M.Bernardi, Darlon I.Pires Junior, Osmindo R.Haddad, Célio F.B. [UNESP]Pedrosa, Carolinada S.G.Souza, Leticia R.Q.Rehen, Stevens K.de Azevedo, Gustavo P.R.Thompson, Cristiane C.Thompson, Fabiano L.Berlinck, Roberto G.S.2022-04-29T08:35:35Z2022-04-29T08:35:35Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article139-146http://dx.doi.org/10.1016/j.toxicon.2021.10.002Toxicon, v. 203, p. 139-146.1879-31500041-0101http://hdl.handle.net/11449/22974310.1016/j.toxicon.2021.10.0022-s2.0-85117376603Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxiconinfo:eu-repo/semantics/openAccess2024-04-09T15:10:36Zoai:repositorio.unesp.br:11449/229743Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:46:31.009784Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga
title Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga
spellingShingle Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga
Chimetto Tonon, Luciane A.
Brachycephalidae
Metabolomics
Metagenomics
Microbiota
Toxin
title_short Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga
title_full Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga
title_fullStr Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga
title_full_unstemmed Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga
title_sort Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga
author Chimetto Tonon, Luciane A.
author_facet Chimetto Tonon, Luciane A.
Rua, Cintia
Crnkovic, Camila M.
Bernardi, Darlon I.
Pires Junior, Osmindo R.
Haddad, Célio F.B. [UNESP]
Pedrosa, Carolinada S.G.
Souza, Leticia R.Q.
Rehen, Stevens K.
de Azevedo, Gustavo P.R.
Thompson, Cristiane C.
Thompson, Fabiano L.
Berlinck, Roberto G.S.
author_role author
author2 Rua, Cintia
Crnkovic, Camila M.
Bernardi, Darlon I.
Pires Junior, Osmindo R.
Haddad, Célio F.B. [UNESP]
Pedrosa, Carolinada S.G.
Souza, Leticia R.Q.
Rehen, Stevens K.
de Azevedo, Gustavo P.R.
Thompson, Cristiane C.
Thompson, Fabiano L.
Berlinck, Roberto G.S.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Federal do Rio de Janeiro (UFRJ)
Universidade de Brasília (UnB)
Universidade Estadual Paulista (UNESP)
Instituto D'Or de Pesquisa e Ensino (IDOR)
dc.contributor.author.fl_str_mv Chimetto Tonon, Luciane A.
Rua, Cintia
Crnkovic, Camila M.
Bernardi, Darlon I.
Pires Junior, Osmindo R.
Haddad, Célio F.B. [UNESP]
Pedrosa, Carolinada S.G.
Souza, Leticia R.Q.
Rehen, Stevens K.
de Azevedo, Gustavo P.R.
Thompson, Cristiane C.
Thompson, Fabiano L.
Berlinck, Roberto G.S.
dc.subject.por.fl_str_mv Brachycephalidae
Metabolomics
Metagenomics
Microbiota
Toxin
topic Brachycephalidae
Metabolomics
Metagenomics
Microbiota
Toxin
description The genus Brachycephalus includes small species of aposematic anurans known as microendemic, occurring in the mountains of the Atlantic Forest. Brachycephalus ephippium, B. nodoterga and B. pernix have been reported to contain the neurotoxin tetrodotoxin in skin and viscera. The biological conservation of several Brachycephalus species is currently threatened by climate change, deforestation, and the pandemic caused by the fungus Batrachochytrium dendrobatidis (Bd). Despite the well-known importance of amphibians’ associated bacteria in the defensive role against pathogens, there is still a poor understanding of amphibian microbiome composition. The present study investigated the composition of B. pitanga microbial community and the presence of TTX in the host and in cultures of bacterial isolates, using a combination of metagenomics, bacterial culture isolation, mass spectrometry and metabolomic analyses. Results of culture-dependent and -independent analyses characterized the microbial communities associated with the skin and viscera of B. pitanga. Mass spectrometry analysis indicated the presence of TTX in host tissues, while bacterial production of TTX was not observed under the experimental conditions used in this investigation. This is the first report confirming the occurrence of TTX in B. pitanga.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-01
2022-04-29T08:35:35Z
2022-04-29T08:35:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.toxicon.2021.10.002
Toxicon, v. 203, p. 139-146.
1879-3150
0041-0101
http://hdl.handle.net/11449/229743
10.1016/j.toxicon.2021.10.002
2-s2.0-85117376603
url http://dx.doi.org/10.1016/j.toxicon.2021.10.002
http://hdl.handle.net/11449/229743
identifier_str_mv Toxicon, v. 203, p. 139-146.
1879-3150
0041-0101
10.1016/j.toxicon.2021.10.002
2-s2.0-85117376603
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxicon
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 139-146
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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