Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.toxicon.2021.10.002 http://hdl.handle.net/11449/229743 |
Resumo: | The genus Brachycephalus includes small species of aposematic anurans known as microendemic, occurring in the mountains of the Atlantic Forest. Brachycephalus ephippium, B. nodoterga and B. pernix have been reported to contain the neurotoxin tetrodotoxin in skin and viscera. The biological conservation of several Brachycephalus species is currently threatened by climate change, deforestation, and the pandemic caused by the fungus Batrachochytrium dendrobatidis (Bd). Despite the well-known importance of amphibians’ associated bacteria in the defensive role against pathogens, there is still a poor understanding of amphibian microbiome composition. The present study investigated the composition of B. pitanga microbial community and the presence of TTX in the host and in cultures of bacterial isolates, using a combination of metagenomics, bacterial culture isolation, mass spectrometry and metabolomic analyses. Results of culture-dependent and -independent analyses characterized the microbial communities associated with the skin and viscera of B. pitanga. Mass spectrometry analysis indicated the presence of TTX in host tissues, while bacterial production of TTX was not observed under the experimental conditions used in this investigation. This is the first report confirming the occurrence of TTX in B. pitanga. |
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Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitangaBrachycephalidaeMetabolomicsMetagenomicsMicrobiotaToxinThe genus Brachycephalus includes small species of aposematic anurans known as microendemic, occurring in the mountains of the Atlantic Forest. Brachycephalus ephippium, B. nodoterga and B. pernix have been reported to contain the neurotoxin tetrodotoxin in skin and viscera. The biological conservation of several Brachycephalus species is currently threatened by climate change, deforestation, and the pandemic caused by the fungus Batrachochytrium dendrobatidis (Bd). Despite the well-known importance of amphibians’ associated bacteria in the defensive role against pathogens, there is still a poor understanding of amphibian microbiome composition. The present study investigated the composition of B. pitanga microbial community and the presence of TTX in the host and in cultures of bacterial isolates, using a combination of metagenomics, bacterial culture isolation, mass spectrometry and metabolomic analyses. Results of culture-dependent and -independent analyses characterized the microbial communities associated with the skin and viscera of B. pitanga. Mass spectrometry analysis indicated the presence of TTX in host tissues, while bacterial production of TTX was not observed under the experimental conditions used in this investigation. This is the first report confirming the occurrence of TTX in B. pitanga.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Instituto de Química de São Carlos Universidade de São Paulo, CP 780Instituto de Biologia SAGE-COPPE Universidade Federal do Rio de Janeiro (UFRJ)Departamento de Tecnologia Bioquímico-Farmacêutica (FBT) Faculdade de Ciências Farmacêuticas Universidade de São PauloLaboratório de Toxinologia Instituto de Biologia Universidade de BrasíliaDepartamento de Biodiversidade e Centro de Aquicultura Instituto de Biociências Universidade Estadual Paulista (UNESP)Instituto D'Or de Pesquisa e Ensino (IDOR)Instituto de Ciências Biomédicas Universidade Federal do Rio de Janeiro (UFRJ)Departamento de Biodiversidade e Centro de Aquicultura Instituto de Biociências Universidade Estadual Paulista (UNESP)FAPESP: 2013/50228-8FAPESP: 2013/50741-7FAPESP: 2016/14375-4FAPESP: 2016/21341-9FAPESP: 2019/00447-1FAPERJ: 202.488/2019Universidade de São Paulo (USP)Universidade Federal do Rio de Janeiro (UFRJ)Universidade de Brasília (UnB)Universidade Estadual Paulista (UNESP)Instituto D'Or de Pesquisa e Ensino (IDOR)Chimetto Tonon, Luciane A.Rua, CintiaCrnkovic, Camila M.Bernardi, Darlon I.Pires Junior, Osmindo R.Haddad, Célio F.B. [UNESP]Pedrosa, Carolinada S.G.Souza, Leticia R.Q.Rehen, Stevens K.de Azevedo, Gustavo P.R.Thompson, Cristiane C.Thompson, Fabiano L.Berlinck, Roberto G.S.2022-04-29T08:35:35Z2022-04-29T08:35:35Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article139-146http://dx.doi.org/10.1016/j.toxicon.2021.10.002Toxicon, v. 203, p. 139-146.1879-31500041-0101http://hdl.handle.net/11449/22974310.1016/j.toxicon.2021.10.0022-s2.0-85117376603Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxiconinfo:eu-repo/semantics/openAccess2024-04-09T15:10:36Zoai:repositorio.unesp.br:11449/229743Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:46:31.009784Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga |
title |
Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga |
spellingShingle |
Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga Chimetto Tonon, Luciane A. Brachycephalidae Metabolomics Metagenomics Microbiota Toxin |
title_short |
Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga |
title_full |
Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga |
title_fullStr |
Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga |
title_full_unstemmed |
Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga |
title_sort |
Microbiome associated with the tetrodotoxin-bearing anuran Brachycephalus pitanga |
author |
Chimetto Tonon, Luciane A. |
author_facet |
Chimetto Tonon, Luciane A. Rua, Cintia Crnkovic, Camila M. Bernardi, Darlon I. Pires Junior, Osmindo R. Haddad, Célio F.B. [UNESP] Pedrosa, Carolinada S.G. Souza, Leticia R.Q. Rehen, Stevens K. de Azevedo, Gustavo P.R. Thompson, Cristiane C. Thompson, Fabiano L. Berlinck, Roberto G.S. |
author_role |
author |
author2 |
Rua, Cintia Crnkovic, Camila M. Bernardi, Darlon I. Pires Junior, Osmindo R. Haddad, Célio F.B. [UNESP] Pedrosa, Carolinada S.G. Souza, Leticia R.Q. Rehen, Stevens K. de Azevedo, Gustavo P.R. Thompson, Cristiane C. Thompson, Fabiano L. Berlinck, Roberto G.S. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal do Rio de Janeiro (UFRJ) Universidade de Brasília (UnB) Universidade Estadual Paulista (UNESP) Instituto D'Or de Pesquisa e Ensino (IDOR) |
dc.contributor.author.fl_str_mv |
Chimetto Tonon, Luciane A. Rua, Cintia Crnkovic, Camila M. Bernardi, Darlon I. Pires Junior, Osmindo R. Haddad, Célio F.B. [UNESP] Pedrosa, Carolinada S.G. Souza, Leticia R.Q. Rehen, Stevens K. de Azevedo, Gustavo P.R. Thompson, Cristiane C. Thompson, Fabiano L. Berlinck, Roberto G.S. |
dc.subject.por.fl_str_mv |
Brachycephalidae Metabolomics Metagenomics Microbiota Toxin |
topic |
Brachycephalidae Metabolomics Metagenomics Microbiota Toxin |
description |
The genus Brachycephalus includes small species of aposematic anurans known as microendemic, occurring in the mountains of the Atlantic Forest. Brachycephalus ephippium, B. nodoterga and B. pernix have been reported to contain the neurotoxin tetrodotoxin in skin and viscera. The biological conservation of several Brachycephalus species is currently threatened by climate change, deforestation, and the pandemic caused by the fungus Batrachochytrium dendrobatidis (Bd). Despite the well-known importance of amphibians’ associated bacteria in the defensive role against pathogens, there is still a poor understanding of amphibian microbiome composition. The present study investigated the composition of B. pitanga microbial community and the presence of TTX in the host and in cultures of bacterial isolates, using a combination of metagenomics, bacterial culture isolation, mass spectrometry and metabolomic analyses. Results of culture-dependent and -independent analyses characterized the microbial communities associated with the skin and viscera of B. pitanga. Mass spectrometry analysis indicated the presence of TTX in host tissues, while bacterial production of TTX was not observed under the experimental conditions used in this investigation. This is the first report confirming the occurrence of TTX in B. pitanga. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-01 2022-04-29T08:35:35Z 2022-04-29T08:35:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.toxicon.2021.10.002 Toxicon, v. 203, p. 139-146. 1879-3150 0041-0101 http://hdl.handle.net/11449/229743 10.1016/j.toxicon.2021.10.002 2-s2.0-85117376603 |
url |
http://dx.doi.org/10.1016/j.toxicon.2021.10.002 http://hdl.handle.net/11449/229743 |
identifier_str_mv |
Toxicon, v. 203, p. 139-146. 1879-3150 0041-0101 10.1016/j.toxicon.2021.10.002 2-s2.0-85117376603 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicon |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
139-146 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128414650990592 |