Strength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regeneration

Detalhes bibliográficos
Autor(a) principal: Morais, Samuel Rodrigues Lourenço [UNESP]
Data de Publicação: 2017
Outros Autores: Goya, Alexandre Ginei [UNESP], Urias, Úrsula, Jannig, Paulo Roberto, Bacurau, Aline Villa Nova, Mello, Wagner Garcez [UNESP], Faleiros, Paula Lazilha [UNESP], Oliveira, Sandra Helena Penha [UNESP], Garcia, Valdir Gouveia [UNESP], Ervolino, Edilson [UNESP], Brum, Patricia Chakur, Dornelles, Rita Cássia Menegati [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10103-016-2116-3
http://hdl.handle.net/11449/176963
Resumo: We evaluated whether strength training (ST) performed prior to skeletal muscle cryolesion would act as a preconditioning, improving skeletal muscle regeneration and responsiveness to low-level laser therapy (LLLT). Wistar rats were randomly assigned into non-exercised (NE), NE plus muscle lesion (NE + LE), NE + LE plus LLLT (NE + LE + LLLT), strength training (ST), ST + LE, and ST + LE + LLLT. The animals performed 10 weeks of ST (climbing ladder; 3× week; 80% overload). Forty-eight hours after the last ST session, tibialis anterior (TA) cryolesion was induced and LLLT (InGaAlP, 660 nm, 0.035 W, 4.9 J/cm2/point, 3 points, spot light 0.028 cm2, 14 J/cm2) initiated and conducted daily for 14 consecutive days. The difference between intergroups was assessed using Student’s t test and intragroups by two-way analysis of variance. Cryolesion induced massive muscle degeneration associated with inflammatory infiltrate. Prior ST improved skeletal regeneration 14-days after cryolesion and potentiated the regenerative response to LLLT. Cryolesion induced increased TNF-α levels in both NE + LE and ST + LE groups. Both isolated ST and LLLT reduced TNF-α to control group levels; however, prior ST potentiated LLLT response. Both isolated ST and LLLT increased IL-10 levels with no additional effect. In contrast, increased TA IL-6 levels were restricted to ST and ST + LE + LLLT groups. TA myogenin mRNA levels were not changed by neither prior ST or ST + LLLT. Both prior ST and LLLT therapies increased MyoD mRNA levels and, interestingly, combined therapies potentiated this response. Myf5 mRNA levels were increased only in ST groups. Taken together, our data provides evidences for prior ST potentiating LLLT efficacy in promoting skeletal muscle regeneration.
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spelling Strength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regenerationLow-level laser therapyMyf5MyoDMyogeninSkeletal muscle regenerationStrength trainingWe evaluated whether strength training (ST) performed prior to skeletal muscle cryolesion would act as a preconditioning, improving skeletal muscle regeneration and responsiveness to low-level laser therapy (LLLT). Wistar rats were randomly assigned into non-exercised (NE), NE plus muscle lesion (NE + LE), NE + LE plus LLLT (NE + LE + LLLT), strength training (ST), ST + LE, and ST + LE + LLLT. The animals performed 10 weeks of ST (climbing ladder; 3× week; 80% overload). Forty-eight hours after the last ST session, tibialis anterior (TA) cryolesion was induced and LLLT (InGaAlP, 660 nm, 0.035 W, 4.9 J/cm2/point, 3 points, spot light 0.028 cm2, 14 J/cm2) initiated and conducted daily for 14 consecutive days. The difference between intergroups was assessed using Student’s t test and intragroups by two-way analysis of variance. Cryolesion induced massive muscle degeneration associated with inflammatory infiltrate. Prior ST improved skeletal regeneration 14-days after cryolesion and potentiated the regenerative response to LLLT. Cryolesion induced increased TNF-α levels in both NE + LE and ST + LE groups. Both isolated ST and LLLT reduced TNF-α to control group levels; however, prior ST potentiated LLLT response. Both isolated ST and LLLT increased IL-10 levels with no additional effect. In contrast, increased TA IL-6 levels were restricted to ST and ST + LE + LLLT groups. TA myogenin mRNA levels were not changed by neither prior ST or ST + LLLT. Both prior ST and LLLT therapies increased MyoD mRNA levels and, interestingly, combined therapies potentiated this response. Myf5 mRNA levels were increased only in ST groups. Taken together, our data provides evidences for prior ST potentiating LLLT efficacy in promoting skeletal muscle regeneration.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas—SBFis/UNESPLaboratory of Endocrine Physiology and Aging—Department of Basic SciencesSão Paulo State University (UNESP) School of DentistrySchool of Physical Education and Sport University of Sao PauloGroup of Research and Study on Laser in Dentistry (GEPLO) Division of Periodontics Department of Surgery and Integrated Clinic São Paulo State University (UNESP) School of DentistryExperimental Physiopathology—Medical School University of Sao PauloPrograma Multicêntrico de Pós-Graduação em Ciências Fisiológicas—SBFis/UNESPSão Paulo State University (UNESP) School of DentistryGroup of Research and Study on Laser in Dentistry (GEPLO) Division of Periodontics Department of Surgery and Integrated Clinic São Paulo State University (UNESP) School of DentistryFAPESP: 2013/18907-2Universidade Estadual Paulista (Unesp)Laboratory of Endocrine Physiology and Aging—Department of Basic SciencesUniversidade de São Paulo (USP)Morais, Samuel Rodrigues Lourenço [UNESP]Goya, Alexandre Ginei [UNESP]Urias, ÚrsulaJannig, Paulo RobertoBacurau, Aline Villa NovaMello, Wagner Garcez [UNESP]Faleiros, Paula Lazilha [UNESP]Oliveira, Sandra Helena Penha [UNESP]Garcia, Valdir Gouveia [UNESP]Ervolino, Edilson [UNESP]Brum, Patricia ChakurDornelles, Rita Cássia Menegati [UNESP]2018-12-11T17:23:17Z2018-12-11T17:23:17Z2017-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article317-325application/pdfhttp://dx.doi.org/10.1007/s10103-016-2116-3Lasers in Medical Science, v. 32, n. 2, p. 317-325, 2017.1435-604X0268-8921http://hdl.handle.net/11449/17696310.1007/s10103-016-2116-32-s2.0-850008854912-s2.0-85000885491.pdf440809551734684654359024227848890000-0003-4859-05830000-0003-0783-6612Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Science0,713info:eu-repo/semantics/openAccess2023-10-05T06:03:33Zoai:repositorio.unesp.br:11449/176963Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-05T06:03:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Strength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regeneration
title Strength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regeneration
spellingShingle Strength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regeneration
Morais, Samuel Rodrigues Lourenço [UNESP]
Low-level laser therapy
Myf5
MyoD
Myogenin
Skeletal muscle regeneration
Strength training
title_short Strength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regeneration
title_full Strength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regeneration
title_fullStr Strength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regeneration
title_full_unstemmed Strength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regeneration
title_sort Strength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regeneration
author Morais, Samuel Rodrigues Lourenço [UNESP]
author_facet Morais, Samuel Rodrigues Lourenço [UNESP]
Goya, Alexandre Ginei [UNESP]
Urias, Úrsula
Jannig, Paulo Roberto
Bacurau, Aline Villa Nova
Mello, Wagner Garcez [UNESP]
Faleiros, Paula Lazilha [UNESP]
Oliveira, Sandra Helena Penha [UNESP]
Garcia, Valdir Gouveia [UNESP]
Ervolino, Edilson [UNESP]
Brum, Patricia Chakur
Dornelles, Rita Cássia Menegati [UNESP]
author_role author
author2 Goya, Alexandre Ginei [UNESP]
Urias, Úrsula
Jannig, Paulo Roberto
Bacurau, Aline Villa Nova
Mello, Wagner Garcez [UNESP]
Faleiros, Paula Lazilha [UNESP]
Oliveira, Sandra Helena Penha [UNESP]
Garcia, Valdir Gouveia [UNESP]
Ervolino, Edilson [UNESP]
Brum, Patricia Chakur
Dornelles, Rita Cássia Menegati [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Laboratory of Endocrine Physiology and Aging—Department of Basic Sciences
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Morais, Samuel Rodrigues Lourenço [UNESP]
Goya, Alexandre Ginei [UNESP]
Urias, Úrsula
Jannig, Paulo Roberto
Bacurau, Aline Villa Nova
Mello, Wagner Garcez [UNESP]
Faleiros, Paula Lazilha [UNESP]
Oliveira, Sandra Helena Penha [UNESP]
Garcia, Valdir Gouveia [UNESP]
Ervolino, Edilson [UNESP]
Brum, Patricia Chakur
Dornelles, Rita Cássia Menegati [UNESP]
dc.subject.por.fl_str_mv Low-level laser therapy
Myf5
MyoD
Myogenin
Skeletal muscle regeneration
Strength training
topic Low-level laser therapy
Myf5
MyoD
Myogenin
Skeletal muscle regeneration
Strength training
description We evaluated whether strength training (ST) performed prior to skeletal muscle cryolesion would act as a preconditioning, improving skeletal muscle regeneration and responsiveness to low-level laser therapy (LLLT). Wistar rats were randomly assigned into non-exercised (NE), NE plus muscle lesion (NE + LE), NE + LE plus LLLT (NE + LE + LLLT), strength training (ST), ST + LE, and ST + LE + LLLT. The animals performed 10 weeks of ST (climbing ladder; 3× week; 80% overload). Forty-eight hours after the last ST session, tibialis anterior (TA) cryolesion was induced and LLLT (InGaAlP, 660 nm, 0.035 W, 4.9 J/cm2/point, 3 points, spot light 0.028 cm2, 14 J/cm2) initiated and conducted daily for 14 consecutive days. The difference between intergroups was assessed using Student’s t test and intragroups by two-way analysis of variance. Cryolesion induced massive muscle degeneration associated with inflammatory infiltrate. Prior ST improved skeletal regeneration 14-days after cryolesion and potentiated the regenerative response to LLLT. Cryolesion induced increased TNF-α levels in both NE + LE and ST + LE groups. Both isolated ST and LLLT reduced TNF-α to control group levels; however, prior ST potentiated LLLT response. Both isolated ST and LLLT increased IL-10 levels with no additional effect. In contrast, increased TA IL-6 levels were restricted to ST and ST + LE + LLLT groups. TA myogenin mRNA levels were not changed by neither prior ST or ST + LLLT. Both prior ST and LLLT therapies increased MyoD mRNA levels and, interestingly, combined therapies potentiated this response. Myf5 mRNA levels were increased only in ST groups. Taken together, our data provides evidences for prior ST potentiating LLLT efficacy in promoting skeletal muscle regeneration.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-01
2018-12-11T17:23:17Z
2018-12-11T17:23:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10103-016-2116-3
Lasers in Medical Science, v. 32, n. 2, p. 317-325, 2017.
1435-604X
0268-8921
http://hdl.handle.net/11449/176963
10.1007/s10103-016-2116-3
2-s2.0-85000885491
2-s2.0-85000885491.pdf
4408095517346846
5435902422784889
0000-0003-4859-0583
0000-0003-0783-6612
url http://dx.doi.org/10.1007/s10103-016-2116-3
http://hdl.handle.net/11449/176963
identifier_str_mv Lasers in Medical Science, v. 32, n. 2, p. 317-325, 2017.
1435-604X
0268-8921
10.1007/s10103-016-2116-3
2-s2.0-85000885491
2-s2.0-85000885491.pdf
4408095517346846
5435902422784889
0000-0003-4859-0583
0000-0003-0783-6612
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lasers in Medical Science
0,713
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 317-325
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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