Low-temperature plasma as an approach for inhibiting a multi-species cariogenic biofilm

Detalhes bibliográficos
Autor(a) principal: Figueira, Leandro W. [UNESP]
Data de Publicação: 2021
Outros Autores: Panariello, Beatriz H. D., Koga-Ito, Cristiane Y. [UNESP], Duarte, Simone
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/app11020570
http://hdl.handle.net/11449/205720
Resumo: This study aimed to determine how low-temperature plasma (LTP) treatment affects single-and multi-species biofilms formed by Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii formed on hydroxyapatite discs. LTP was produced by argon gas using the kIN-Pen09™ (Leibniz Institute for Plasma Science and Technology, INP, Greifswald, Germany). Biofilms were treated at a 10 mm distance from the nozzle of the plasma device to the surface of the biofilm per 30 s, 60 s, and 120 s. A 0.89% saline solution and a 0.12% chlorhexidine solution were used as negative and positive controls, respectively. Argon flow at three exposure times (30 s, 60 s, and 120 s) was also used as control. Biofilm viability was analyzed by colony-forming units (CFU) recovery and confocal laser scanning microscopy. Multispecies biofilms presented a reduction in viability (log10 CFU/mL) for all plasma-treated samples when compared to both positive and negative controls (p < 0.0001). In single-species biofilms formed by either S. mutans or S. sanguinis, a significant reduction in all exposure times was observed when compared to both positive and negative controls (p < 0.0001). For single-species biofilms formed by S. gordonii, the results indicate total elimination of S. gordonii for all exposure times. Low exposure times of LTP affects single-and multi-species cariogenic biofilms, which indicates that the treatment is a promising source for the development of new protocols for the control of dental caries.
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spelling Low-temperature plasma as an approach for inhibiting a multi-species cariogenic biofilmAntibacterialDental cariesStreptococcus gordoniiStreptococcus mutansStreptococcus sanguinisThis study aimed to determine how low-temperature plasma (LTP) treatment affects single-and multi-species biofilms formed by Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii formed on hydroxyapatite discs. LTP was produced by argon gas using the kIN-Pen09™ (Leibniz Institute for Plasma Science and Technology, INP, Greifswald, Germany). Biofilms were treated at a 10 mm distance from the nozzle of the plasma device to the surface of the biofilm per 30 s, 60 s, and 120 s. A 0.89% saline solution and a 0.12% chlorhexidine solution were used as negative and positive controls, respectively. Argon flow at three exposure times (30 s, 60 s, and 120 s) was also used as control. Biofilm viability was analyzed by colony-forming units (CFU) recovery and confocal laser scanning microscopy. Multispecies biofilms presented a reduction in viability (log10 CFU/mL) for all plasma-treated samples when compared to both positive and negative controls (p < 0.0001). In single-species biofilms formed by either S. mutans or S. sanguinis, a significant reduction in all exposure times was observed when compared to both positive and negative controls (p < 0.0001). For single-species biofilms formed by S. gordonii, the results indicate total elimination of S. gordonii for all exposure times. Low exposure times of LTP affects single-and multi-species cariogenic biofilms, which indicates that the treatment is a promising source for the development of new protocols for the control of dental caries.National Institutes of HealthFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Institute of Science and Technology São Paulo State University UNESPDepartment of Cariology Operative Dentistry and Dental Public Health Indiana University School of DentistryInstitute of Science and Technology São Paulo State University UNESPNational Institutes of Health: 1R21DE028929-01FAPESP: 2019/01676-4FAPESP: 2019/05856-7Universidade Estadual Paulista (Unesp)Indiana University School of DentistryFigueira, Leandro W. [UNESP]Panariello, Beatriz H. D.Koga-Ito, Cristiane Y. [UNESP]Duarte, Simone2021-06-25T10:20:09Z2021-06-25T10:20:09Z2021-01-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-12http://dx.doi.org/10.3390/app11020570Applied Sciences (Switzerland), v. 11, n. 2, p. 1-12, 2021.2076-3417http://hdl.handle.net/11449/20572010.3390/app110205702-s2.0-8509925677165435631614034210000-0002-2416-2173Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied Sciences (Switzerland)info:eu-repo/semantics/openAccess2022-03-15T18:13:56Zoai:repositorio.unesp.br:11449/205720Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:41:48.300588Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Low-temperature plasma as an approach for inhibiting a multi-species cariogenic biofilm
title Low-temperature plasma as an approach for inhibiting a multi-species cariogenic biofilm
spellingShingle Low-temperature plasma as an approach for inhibiting a multi-species cariogenic biofilm
Figueira, Leandro W. [UNESP]
Antibacterial
Dental caries
Streptococcus gordonii
Streptococcus mutans
Streptococcus sanguinis
title_short Low-temperature plasma as an approach for inhibiting a multi-species cariogenic biofilm
title_full Low-temperature plasma as an approach for inhibiting a multi-species cariogenic biofilm
title_fullStr Low-temperature plasma as an approach for inhibiting a multi-species cariogenic biofilm
title_full_unstemmed Low-temperature plasma as an approach for inhibiting a multi-species cariogenic biofilm
title_sort Low-temperature plasma as an approach for inhibiting a multi-species cariogenic biofilm
author Figueira, Leandro W. [UNESP]
author_facet Figueira, Leandro W. [UNESP]
Panariello, Beatriz H. D.
Koga-Ito, Cristiane Y. [UNESP]
Duarte, Simone
author_role author
author2 Panariello, Beatriz H. D.
Koga-Ito, Cristiane Y. [UNESP]
Duarte, Simone
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Indiana University School of Dentistry
dc.contributor.author.fl_str_mv Figueira, Leandro W. [UNESP]
Panariello, Beatriz H. D.
Koga-Ito, Cristiane Y. [UNESP]
Duarte, Simone
dc.subject.por.fl_str_mv Antibacterial
Dental caries
Streptococcus gordonii
Streptococcus mutans
Streptococcus sanguinis
topic Antibacterial
Dental caries
Streptococcus gordonii
Streptococcus mutans
Streptococcus sanguinis
description This study aimed to determine how low-temperature plasma (LTP) treatment affects single-and multi-species biofilms formed by Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii formed on hydroxyapatite discs. LTP was produced by argon gas using the kIN-Pen09™ (Leibniz Institute for Plasma Science and Technology, INP, Greifswald, Germany). Biofilms were treated at a 10 mm distance from the nozzle of the plasma device to the surface of the biofilm per 30 s, 60 s, and 120 s. A 0.89% saline solution and a 0.12% chlorhexidine solution were used as negative and positive controls, respectively. Argon flow at three exposure times (30 s, 60 s, and 120 s) was also used as control. Biofilm viability was analyzed by colony-forming units (CFU) recovery and confocal laser scanning microscopy. Multispecies biofilms presented a reduction in viability (log10 CFU/mL) for all plasma-treated samples when compared to both positive and negative controls (p < 0.0001). In single-species biofilms formed by either S. mutans or S. sanguinis, a significant reduction in all exposure times was observed when compared to both positive and negative controls (p < 0.0001). For single-species biofilms formed by S. gordonii, the results indicate total elimination of S. gordonii for all exposure times. Low exposure times of LTP affects single-and multi-species cariogenic biofilms, which indicates that the treatment is a promising source for the development of new protocols for the control of dental caries.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:20:09Z
2021-06-25T10:20:09Z
2021-01-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/app11020570
Applied Sciences (Switzerland), v. 11, n. 2, p. 1-12, 2021.
2076-3417
http://hdl.handle.net/11449/205720
10.3390/app11020570
2-s2.0-85099256771
6543563161403421
0000-0002-2416-2173
url http://dx.doi.org/10.3390/app11020570
http://hdl.handle.net/11449/205720
identifier_str_mv Applied Sciences (Switzerland), v. 11, n. 2, p. 1-12, 2021.
2076-3417
10.3390/app11020570
2-s2.0-85099256771
6543563161403421
0000-0002-2416-2173
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Applied Sciences (Switzerland)
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-12
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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