Possible origin of B chromosome in Dichotomius sericeus (Coleoptera)
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1139/gen-2016-0048 http://hdl.handle.net/11449/161812 |
Resumo: | B chromosomes have so far been described in about 80 species of Coleoptera, mainly using conventional staining analysis. In this study, 152 individuals of the dung beetle Dichotomius sericeus (Coleoptera), collected from three isolated geographical areas in the State of Pernambuco, Brazil, were analyzed to determine the frequency, prevalence, distribution, meiotic behavior, and possible B chromosome origin. The cytogenetic analysis consisted of conventional staining, C-banding, triple fluorochrome staining (CMA3/DA/DAPI), and fluorescent in situ hybridization using ribosomal DNAs (rDNAs) and H3 histone gene as probes, as well as microdissection and chromosome painting of the B chromosome. The B chromosomes were detected in all populations analyzed. Analysis revealed the heterochromatic nature and the presence of G+C-rich blocks and 18S rDNA on the B chromosome. FISH with DNA from microdissected B chromosome painted the entire extension of the B chromosome for all populations, besides the pericentromeric regions of all the autosomes, as well as the X chromosome. Finally, cross-hybridization in nine related species of Dichotomius using the microdissected B chromosome as probe did not reveal any hybridization signal. The results suggest an intraspecific and monophyletic origin for B chromosomes in D. sericeus, probably from the second or third autosomal pair. |
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Possible origin of B chromosome in Dichotomius sericeus (Coleoptera)chromosome paintingchromosome evolutionhistonerDNAB chromosomes have so far been described in about 80 species of Coleoptera, mainly using conventional staining analysis. In this study, 152 individuals of the dung beetle Dichotomius sericeus (Coleoptera), collected from three isolated geographical areas in the State of Pernambuco, Brazil, were analyzed to determine the frequency, prevalence, distribution, meiotic behavior, and possible B chromosome origin. The cytogenetic analysis consisted of conventional staining, C-banding, triple fluorochrome staining (CMA3/DA/DAPI), and fluorescent in situ hybridization using ribosomal DNAs (rDNAs) and H3 histone gene as probes, as well as microdissection and chromosome painting of the B chromosome. The B chromosomes were detected in all populations analyzed. Analysis revealed the heterochromatic nature and the presence of G+C-rich blocks and 18S rDNA on the B chromosome. FISH with DNA from microdissected B chromosome painted the entire extension of the B chromosome for all populations, besides the pericentromeric regions of all the autosomes, as well as the X chromosome. Finally, cross-hybridization in nine related species of Dichotomius using the microdissected B chromosome as probe did not reveal any hybridization signal. The results suggest an intraspecific and monophyletic origin for B chromosomes in D. sericeus, probably from the second or third autosomal pair.Fundacao de Amparo a Ciencia e Tecnologia de Pernambuco (FACEPE)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Pernambuco, Inst Ciencias Biol, Lab Biodiversidade & Genet Insetos, Recife, PE, BrazilUniv Estadual Paulista, Inst Ciencias, Dept Biol, Rio Claro, BrazilUniv Estadual Paulista, Inst Ciencias, Dept Biol, Rio Claro, BrazilFundacao de Amparo a Ciencia e Tecnologia de Pernambuco (FACEPE): IBPG-0742-2.02/11FAPESP: 2011/19481-3CNPq: CNPq 475308/2011-5Canadian Science Publishing, Nrc Research PressUniv PernambucoUniversidade Estadual Paulista (Unesp)Amorim, Igor CostaMilani, Diogo [UNESP]Cabral-de-Mello, Diogo Cavalcanti [UNESP]Rocha, Marilia FrancaMoura, Rita Cassia2018-11-26T16:54:55Z2018-11-26T16:54:55Z2016-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article575-580http://dx.doi.org/10.1139/gen-2016-0048Genome. Ottawa: Canadian Science Publishing, Nrc Research Press, v. 59, n. 8, p. 575-580, 2016.0831-2796http://hdl.handle.net/11449/16181210.1139/gen-2016-0048WOS:000381228000005Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenome0,655info:eu-repo/semantics/openAccess2024-10-17T18:19:26Zoai:repositorio.unesp.br:11449/161812Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-10-17T18:19:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Possible origin of B chromosome in Dichotomius sericeus (Coleoptera) |
title |
Possible origin of B chromosome in Dichotomius sericeus (Coleoptera) |
spellingShingle |
Possible origin of B chromosome in Dichotomius sericeus (Coleoptera) Amorim, Igor Costa chromosome painting chromosome evolution histone rDNA |
title_short |
Possible origin of B chromosome in Dichotomius sericeus (Coleoptera) |
title_full |
Possible origin of B chromosome in Dichotomius sericeus (Coleoptera) |
title_fullStr |
Possible origin of B chromosome in Dichotomius sericeus (Coleoptera) |
title_full_unstemmed |
Possible origin of B chromosome in Dichotomius sericeus (Coleoptera) |
title_sort |
Possible origin of B chromosome in Dichotomius sericeus (Coleoptera) |
author |
Amorim, Igor Costa |
author_facet |
Amorim, Igor Costa Milani, Diogo [UNESP] Cabral-de-Mello, Diogo Cavalcanti [UNESP] Rocha, Marilia Franca Moura, Rita Cassia |
author_role |
author |
author2 |
Milani, Diogo [UNESP] Cabral-de-Mello, Diogo Cavalcanti [UNESP] Rocha, Marilia Franca Moura, Rita Cassia |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Univ Pernambuco Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Amorim, Igor Costa Milani, Diogo [UNESP] Cabral-de-Mello, Diogo Cavalcanti [UNESP] Rocha, Marilia Franca Moura, Rita Cassia |
dc.subject.por.fl_str_mv |
chromosome painting chromosome evolution histone rDNA |
topic |
chromosome painting chromosome evolution histone rDNA |
description |
B chromosomes have so far been described in about 80 species of Coleoptera, mainly using conventional staining analysis. In this study, 152 individuals of the dung beetle Dichotomius sericeus (Coleoptera), collected from three isolated geographical areas in the State of Pernambuco, Brazil, were analyzed to determine the frequency, prevalence, distribution, meiotic behavior, and possible B chromosome origin. The cytogenetic analysis consisted of conventional staining, C-banding, triple fluorochrome staining (CMA3/DA/DAPI), and fluorescent in situ hybridization using ribosomal DNAs (rDNAs) and H3 histone gene as probes, as well as microdissection and chromosome painting of the B chromosome. The B chromosomes were detected in all populations analyzed. Analysis revealed the heterochromatic nature and the presence of G+C-rich blocks and 18S rDNA on the B chromosome. FISH with DNA from microdissected B chromosome painted the entire extension of the B chromosome for all populations, besides the pericentromeric regions of all the autosomes, as well as the X chromosome. Finally, cross-hybridization in nine related species of Dichotomius using the microdissected B chromosome as probe did not reveal any hybridization signal. The results suggest an intraspecific and monophyletic origin for B chromosomes in D. sericeus, probably from the second or third autosomal pair. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-01 2018-11-26T16:54:55Z 2018-11-26T16:54:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1139/gen-2016-0048 Genome. Ottawa: Canadian Science Publishing, Nrc Research Press, v. 59, n. 8, p. 575-580, 2016. 0831-2796 http://hdl.handle.net/11449/161812 10.1139/gen-2016-0048 WOS:000381228000005 |
url |
http://dx.doi.org/10.1139/gen-2016-0048 http://hdl.handle.net/11449/161812 |
identifier_str_mv |
Genome. Ottawa: Canadian Science Publishing, Nrc Research Press, v. 59, n. 8, p. 575-580, 2016. 0831-2796 10.1139/gen-2016-0048 WOS:000381228000005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genome 0,655 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
575-580 |
dc.publisher.none.fl_str_mv |
Canadian Science Publishing, Nrc Research Press |
publisher.none.fl_str_mv |
Canadian Science Publishing, Nrc Research Press |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1826304160982630400 |