Direct and simultaneous determination of Cd, Cu, and Se in blood samples by graphite furnace atomic absorption spectrometry
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/225677 |
Resumo: | A graphite furnace atomic absorption speclromclric method is proposed for the direct and simultaneous determination of Cd. Cu. and Se in human blood. Samples were diluted 1:10 (v/v) in 0.6% (v/v) UNO3 + 0.6% (v/v) Triton X-100 solution. Tor 12 μL injected sample volume + 6 μL of 1000 mg L-1 Pd(NO3)2+ 3 μL of 1000 mg L-1 Mg(NO3)2. the calculated characteristic masses (mo) were 0.9 pg Cd. 16 pg Cu. and 39 pg Se. which are close to those mo values for single-element conditions for THGA furnace (1.3 pg Cd, 17 pg Cu. and 16 pg Se). Calibration curves with linear correlations better than 0.999 were obtained. The limits of detection (LOD) were 0.03 μg L-1 Cd, 0.075 μg L -1 Cu and 0.3 μg L-1 Se, and the relative standard deviations (n= 12) were 2.6%. 0.3%. and 1.6%. respectively. The method was applied for Cd, Cu, and Se determination in 10 human blood samples and the results were in agreement at the 96% confidence level with those obtained by inductively coupled plasma mass spectrometry. Concentrations of analytes in the selected blood samples varied from 1.7 to 3.2 μg L-1 Cd. 700 to 921.7 μg L-1 Cu. and from 68.6 to 360 μg L-1 Se. The accuracy of the proposed method was also evaluated by an additionrecovery experiment and recoveries of Cd, Cu, and Se added to blood samples ranged from 99-109%. 91-103%. and 93-103%. respectively. |
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Repositório Institucional da UNESP |
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Direct and simultaneous determination of Cd, Cu, and Se in blood samples by graphite furnace atomic absorption spectrometryA graphite furnace atomic absorption speclromclric method is proposed for the direct and simultaneous determination of Cd. Cu. and Se in human blood. Samples were diluted 1:10 (v/v) in 0.6% (v/v) UNO3 + 0.6% (v/v) Triton X-100 solution. Tor 12 μL injected sample volume + 6 μL of 1000 mg L-1 Pd(NO3)2+ 3 μL of 1000 mg L-1 Mg(NO3)2. the calculated characteristic masses (mo) were 0.9 pg Cd. 16 pg Cu. and 39 pg Se. which are close to those mo values for single-element conditions for THGA furnace (1.3 pg Cd, 17 pg Cu. and 16 pg Se). Calibration curves with linear correlations better than 0.999 were obtained. The limits of detection (LOD) were 0.03 μg L-1 Cd, 0.075 μg L -1 Cu and 0.3 μg L-1 Se, and the relative standard deviations (n= 12) were 2.6%. 0.3%. and 1.6%. respectively. The method was applied for Cd, Cu, and Se determination in 10 human blood samples and the results were in agreement at the 96% confidence level with those obtained by inductively coupled plasma mass spectrometry. Concentrations of analytes in the selected blood samples varied from 1.7 to 3.2 μg L-1 Cd. 700 to 921.7 μg L-1 Cu. and from 68.6 to 360 μg L-1 Se. The accuracy of the proposed method was also evaluated by an additionrecovery experiment and recoveries of Cd, Cu, and Se added to blood samples ranged from 99-109%. 91-103%. and 93-103%. respectively.Piauí Federal University - UFPI Chemistry Department Campus Ministro Petrônio Portella, 64049-550, Teresina-PISão Paulo State University - UNESP Institute of Chemistry Analytical Chemistry Department, P.O. Box 355, 14801-970, Araraquara-SPUniversity of São Paulo Faculty of Pharmaceutical Sciences of Ribeirão Preto, 14040-903 Ribeirão Preto-SPSão Paulo State University - UNESP Institute of Chemistry Analytical Chemistry Department, P.O. Box 355, 14801-970, Araraquara-SPChemistry DepartmentUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Caldas, Naise MaryRaposo, Jorge Luiz [UNESP]Oliveira, Silvana Ruella [UNESP]Neto, José Anchieta Gomes [UNESP]Barbosa, Fernando2022-04-28T20:57:44Z2022-04-28T20:57:44Z2009-11-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article115-119Atomic Spectroscopy, v. 30, n. 4, p. 115-119, 2009.0195-5373http://hdl.handle.net/11449/2256772-s2.0-70450162631Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAtomic Spectroscopyinfo:eu-repo/semantics/openAccess2022-04-28T20:57:44Zoai:repositorio.unesp.br:11449/225677Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:39:34.379132Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Direct and simultaneous determination of Cd, Cu, and Se in blood samples by graphite furnace atomic absorption spectrometry |
title |
Direct and simultaneous determination of Cd, Cu, and Se in blood samples by graphite furnace atomic absorption spectrometry |
spellingShingle |
Direct and simultaneous determination of Cd, Cu, and Se in blood samples by graphite furnace atomic absorption spectrometry Caldas, Naise Mary |
title_short |
Direct and simultaneous determination of Cd, Cu, and Se in blood samples by graphite furnace atomic absorption spectrometry |
title_full |
Direct and simultaneous determination of Cd, Cu, and Se in blood samples by graphite furnace atomic absorption spectrometry |
title_fullStr |
Direct and simultaneous determination of Cd, Cu, and Se in blood samples by graphite furnace atomic absorption spectrometry |
title_full_unstemmed |
Direct and simultaneous determination of Cd, Cu, and Se in blood samples by graphite furnace atomic absorption spectrometry |
title_sort |
Direct and simultaneous determination of Cd, Cu, and Se in blood samples by graphite furnace atomic absorption spectrometry |
author |
Caldas, Naise Mary |
author_facet |
Caldas, Naise Mary Raposo, Jorge Luiz [UNESP] Oliveira, Silvana Ruella [UNESP] Neto, José Anchieta Gomes [UNESP] Barbosa, Fernando |
author_role |
author |
author2 |
Raposo, Jorge Luiz [UNESP] Oliveira, Silvana Ruella [UNESP] Neto, José Anchieta Gomes [UNESP] Barbosa, Fernando |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Chemistry Department Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Caldas, Naise Mary Raposo, Jorge Luiz [UNESP] Oliveira, Silvana Ruella [UNESP] Neto, José Anchieta Gomes [UNESP] Barbosa, Fernando |
description |
A graphite furnace atomic absorption speclromclric method is proposed for the direct and simultaneous determination of Cd. Cu. and Se in human blood. Samples were diluted 1:10 (v/v) in 0.6% (v/v) UNO3 + 0.6% (v/v) Triton X-100 solution. Tor 12 μL injected sample volume + 6 μL of 1000 mg L-1 Pd(NO3)2+ 3 μL of 1000 mg L-1 Mg(NO3)2. the calculated characteristic masses (mo) were 0.9 pg Cd. 16 pg Cu. and 39 pg Se. which are close to those mo values for single-element conditions for THGA furnace (1.3 pg Cd, 17 pg Cu. and 16 pg Se). Calibration curves with linear correlations better than 0.999 were obtained. The limits of detection (LOD) were 0.03 μg L-1 Cd, 0.075 μg L -1 Cu and 0.3 μg L-1 Se, and the relative standard deviations (n= 12) were 2.6%. 0.3%. and 1.6%. respectively. The method was applied for Cd, Cu, and Se determination in 10 human blood samples and the results were in agreement at the 96% confidence level with those obtained by inductively coupled plasma mass spectrometry. Concentrations of analytes in the selected blood samples varied from 1.7 to 3.2 μg L-1 Cd. 700 to 921.7 μg L-1 Cu. and from 68.6 to 360 μg L-1 Se. The accuracy of the proposed method was also evaluated by an additionrecovery experiment and recoveries of Cd, Cu, and Se added to blood samples ranged from 99-109%. 91-103%. and 93-103%. respectively. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-11-26 2022-04-28T20:57:44Z 2022-04-28T20:57:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Atomic Spectroscopy, v. 30, n. 4, p. 115-119, 2009. 0195-5373 http://hdl.handle.net/11449/225677 2-s2.0-70450162631 |
identifier_str_mv |
Atomic Spectroscopy, v. 30, n. 4, p. 115-119, 2009. 0195-5373 2-s2.0-70450162631 |
url |
http://hdl.handle.net/11449/225677 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Atomic Spectroscopy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
115-119 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129345253801984 |