Overexpression of the activated form of the AtAREB1 gene (AtAREB1 Delta QT) improves soybean responses to water deficit

Detalhes bibliográficos
Autor(a) principal: Leite, J. P. [UNESP]
Data de Publicação: 2014
Outros Autores: Barbosa, E. G. G., Marin, S. R. R., Marinho, J. P., Carvalho, J. F. C., Pagliarini, R. F., Cruz, A. S., Oliveira, M. C. N., Farias, J. R. B., Neumaier, N., Guimaraes, F. C. M., Yoshida, T., Kanamori, N., Fujita, Y., Nakashima, K., Shinozaki, K. Y., Desiderio, J. A. [UNESP], Nepomuceno, A. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4238/2014.August.15.10
http://hdl.handle.net/11449/117559
Resumo: Abscisic acid-responsive element binding protein (AREB1) is a basic domain/leucine zipper transcription factor that binds to the abscisic acid (ABA)-responsive element motif in the promoter region of ABA-inducible genes. Because AREB1 is not sufficient to direct the expression of downstream genes under non-stress conditions, an activated form of AREB1 (AREB1 Delta QT) was created. Several reports claim that plants overexpressing AREB1 or AREB1 Delta QT show improved drought tolerance. In our studies, soybean plants overexpressing AREB1 Delta QT were characterized molecularly, and the phenotype and drought response of three lines were accessed under greenhouse conditions. Under conditions of water deficit, the transformed plants presented a higher survival rate (100%) than those of their isoline, cultivar BR 16 (40%). Moreover, the transformed plants displayed better water use efficiency and had a higher number of leaves than their isoline. Because the transgenic plants had higher stomatal conductance than its isoline under well-watered conditions, it was suggested that the enhanced drought response of AREB1 Delta QT soybean plants might not be associated with altered transpiration rates mediated by ABA-dependent stomatal closure. However, it is possible that the smaller leaf area of the transgenic plants reduced their transpiration and water use, causing delayed stress onset. The difference in the degree of wilting and percentage of survival between the 35S-AREB1 Delta QT and wildtype plants may also be related to the regulation of genes that protect against dehydration because metabolic impairment of photosynthesis, deduced by an increasing internal CO2 concentration, was not observed in the transgenic plants.
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spelling Overexpression of the activated form of the AtAREB1 gene (AtAREB1 Delta QT) improves soybean responses to water deficitAtAREB1 Delta QTBiolisticsDrought toleranceSoybeanTranscription factorWater use efficiencyAbscisic acid-responsive element binding protein (AREB1) is a basic domain/leucine zipper transcription factor that binds to the abscisic acid (ABA)-responsive element motif in the promoter region of ABA-inducible genes. Because AREB1 is not sufficient to direct the expression of downstream genes under non-stress conditions, an activated form of AREB1 (AREB1 Delta QT) was created. Several reports claim that plants overexpressing AREB1 or AREB1 Delta QT show improved drought tolerance. In our studies, soybean plants overexpressing AREB1 Delta QT were characterized molecularly, and the phenotype and drought response of three lines were accessed under greenhouse conditions. Under conditions of water deficit, the transformed plants presented a higher survival rate (100%) than those of their isoline, cultivar BR 16 (40%). Moreover, the transformed plants displayed better water use efficiency and had a higher number of leaves than their isoline. Because the transgenic plants had higher stomatal conductance than its isoline under well-watered conditions, it was suggested that the enhanced drought response of AREB1 Delta QT soybean plants might not be associated with altered transpiration rates mediated by ABA-dependent stomatal closure. However, it is possible that the smaller leaf area of the transgenic plants reduced their transpiration and water use, causing delayed stress onset. The difference in the degree of wilting and percentage of survival between the 35S-AREB1 Delta QT and wildtype plants may also be related to the regulation of genes that protect against dehydration because metabolic impairment of photosynthesis, deduced by an increasing internal CO2 concentration, was not observed in the transgenic plants.Science and Technology Research Partnership for Sustainable Development (SATREPS) of the Japan Science and Technology Agency/Japan International Cooperation Agency (JIRCAS)Empresa Brasileira de Pesquisa AgropecuariaCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Faculdade de Ciencias Agrarias e VeterinariaUniversidade Estadual Paulista (FCAV-UNESP), Jaboticabal, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Biol Aplicada Agr, Jaboticabal, SP, BrazilEmbrapa Soja, Lab Biotecnol Vegetal, Londrina, PR, BrazilUniv Estadual Londrina, Dept Biol Geral, Londrina, PR, BrazilEmbrapa Soja, Dept Biometr, Londrina, PR, BrazilEmbrapa Soja, Lab Ecofisiol Vegetal, Londrina, PR, BrazilJapan Int Res Ctr Agr Sci, Biol Resources & Postharvest Div, Tsukuba, Ibaraki, JapanUniv Tokyo, Bunkyo Ku, Lab Plant Mol Physiol, Tokyo 113, JapanUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Biol Aplicada Agr, Jaboticabal, SP, BrazilFunpec-editoraUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual de Londrina (UEL)Japan Int Res Ctr Agr SciUniv TokyoLeite, J. P. [UNESP]Barbosa, E. G. G.Marin, S. R. R.Marinho, J. P.Carvalho, J. F. C.Pagliarini, R. F.Cruz, A. S.Oliveira, M. C. N.Farias, J. R. B.Neumaier, N.Guimaraes, F. C. M.Yoshida, T.Kanamori, N.Fujita, Y.Nakashima, K.Shinozaki, K. Y.Desiderio, J. A. [UNESP]Nepomuceno, A. L.2015-03-18T15:56:26Z2015-03-18T15:56:26Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6272-6286application/pdfhttp://dx.doi.org/10.4238/2014.August.15.10Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 13, n. 3, p. 6272-6286, 2014.1676-5680http://hdl.handle.net/11449/11755910.4238/2014.August.15.10WOS:000340383600167WOS000340383600167.pdf1295051340591836Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics And Molecular Research0,439info:eu-repo/semantics/openAccess2024-06-06T13:04:56Zoai:repositorio.unesp.br:11449/117559Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:25:53.932483Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Overexpression of the activated form of the AtAREB1 gene (AtAREB1 Delta QT) improves soybean responses to water deficit
title Overexpression of the activated form of the AtAREB1 gene (AtAREB1 Delta QT) improves soybean responses to water deficit
spellingShingle Overexpression of the activated form of the AtAREB1 gene (AtAREB1 Delta QT) improves soybean responses to water deficit
Leite, J. P. [UNESP]
AtAREB1 Delta QT
Biolistics
Drought tolerance
Soybean
Transcription factor
Water use efficiency
title_short Overexpression of the activated form of the AtAREB1 gene (AtAREB1 Delta QT) improves soybean responses to water deficit
title_full Overexpression of the activated form of the AtAREB1 gene (AtAREB1 Delta QT) improves soybean responses to water deficit
title_fullStr Overexpression of the activated form of the AtAREB1 gene (AtAREB1 Delta QT) improves soybean responses to water deficit
title_full_unstemmed Overexpression of the activated form of the AtAREB1 gene (AtAREB1 Delta QT) improves soybean responses to water deficit
title_sort Overexpression of the activated form of the AtAREB1 gene (AtAREB1 Delta QT) improves soybean responses to water deficit
author Leite, J. P. [UNESP]
author_facet Leite, J. P. [UNESP]
Barbosa, E. G. G.
Marin, S. R. R.
Marinho, J. P.
Carvalho, J. F. C.
Pagliarini, R. F.
Cruz, A. S.
Oliveira, M. C. N.
Farias, J. R. B.
Neumaier, N.
Guimaraes, F. C. M.
Yoshida, T.
Kanamori, N.
Fujita, Y.
Nakashima, K.
Shinozaki, K. Y.
Desiderio, J. A. [UNESP]
Nepomuceno, A. L.
author_role author
author2 Barbosa, E. G. G.
Marin, S. R. R.
Marinho, J. P.
Carvalho, J. F. C.
Pagliarini, R. F.
Cruz, A. S.
Oliveira, M. C. N.
Farias, J. R. B.
Neumaier, N.
Guimaraes, F. C. M.
Yoshida, T.
Kanamori, N.
Fujita, Y.
Nakashima, K.
Shinozaki, K. Y.
Desiderio, J. A. [UNESP]
Nepomuceno, A. L.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Estadual de Londrina (UEL)
Japan Int Res Ctr Agr Sci
Univ Tokyo
dc.contributor.author.fl_str_mv Leite, J. P. [UNESP]
Barbosa, E. G. G.
Marin, S. R. R.
Marinho, J. P.
Carvalho, J. F. C.
Pagliarini, R. F.
Cruz, A. S.
Oliveira, M. C. N.
Farias, J. R. B.
Neumaier, N.
Guimaraes, F. C. M.
Yoshida, T.
Kanamori, N.
Fujita, Y.
Nakashima, K.
Shinozaki, K. Y.
Desiderio, J. A. [UNESP]
Nepomuceno, A. L.
dc.subject.por.fl_str_mv AtAREB1 Delta QT
Biolistics
Drought tolerance
Soybean
Transcription factor
Water use efficiency
topic AtAREB1 Delta QT
Biolistics
Drought tolerance
Soybean
Transcription factor
Water use efficiency
description Abscisic acid-responsive element binding protein (AREB1) is a basic domain/leucine zipper transcription factor that binds to the abscisic acid (ABA)-responsive element motif in the promoter region of ABA-inducible genes. Because AREB1 is not sufficient to direct the expression of downstream genes under non-stress conditions, an activated form of AREB1 (AREB1 Delta QT) was created. Several reports claim that plants overexpressing AREB1 or AREB1 Delta QT show improved drought tolerance. In our studies, soybean plants overexpressing AREB1 Delta QT were characterized molecularly, and the phenotype and drought response of three lines were accessed under greenhouse conditions. Under conditions of water deficit, the transformed plants presented a higher survival rate (100%) than those of their isoline, cultivar BR 16 (40%). Moreover, the transformed plants displayed better water use efficiency and had a higher number of leaves than their isoline. Because the transgenic plants had higher stomatal conductance than its isoline under well-watered conditions, it was suggested that the enhanced drought response of AREB1 Delta QT soybean plants might not be associated with altered transpiration rates mediated by ABA-dependent stomatal closure. However, it is possible that the smaller leaf area of the transgenic plants reduced their transpiration and water use, causing delayed stress onset. The difference in the degree of wilting and percentage of survival between the 35S-AREB1 Delta QT and wildtype plants may also be related to the regulation of genes that protect against dehydration because metabolic impairment of photosynthesis, deduced by an increasing internal CO2 concentration, was not observed in the transgenic plants.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
2015-03-18T15:56:26Z
2015-03-18T15:56:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4238/2014.August.15.10
Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 13, n. 3, p. 6272-6286, 2014.
1676-5680
http://hdl.handle.net/11449/117559
10.4238/2014.August.15.10
WOS:000340383600167
WOS000340383600167.pdf
1295051340591836
url http://dx.doi.org/10.4238/2014.August.15.10
http://hdl.handle.net/11449/117559
identifier_str_mv Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 13, n. 3, p. 6272-6286, 2014.
1676-5680
10.4238/2014.August.15.10
WOS:000340383600167
WOS000340383600167.pdf
1295051340591836
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics And Molecular Research
0,439
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 6272-6286
application/pdf
dc.publisher.none.fl_str_mv Funpec-editora
publisher.none.fl_str_mv Funpec-editora
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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