Role of insulin-like growth factor 1 on cross-bred Bos indicus cattle germinal vesicle oocytes exposed to heat shock
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1071/RD15514 http://hdl.handle.net/11449/174780 |
Resumo: | Germinal vesicle (GV) oocytes are susceptible to heat stress. However, neither the cellular mechanisms triggered by elevated temperature nor the thermoprotective effects of insulin-like growth factor (IGF) on GV oocytes are completely understood. Therefore, a series of experiments was conducted to determine the direct effects of IGF1 (0, 12.5, 25, 50 and 100ng mL-1) on heat-treated GV oocytes. Butyrolactone-arrested GV oocytes were cultured at 38.5°C (control) or 41°C (heat shock; HS) for 14h in the presence of different concentrations of IGF1. Exposure of GV oocytes to 41°C increased (P<0.05) the number of terminal deoxyribonucleotidyl transferase-mediated fluorescein-dUTP nick end-labelling (TUNEL)-positive oocytes. At concentrations of 12.5 and 25ng mL-1, IGF1 tended to minimise these negative effect of HS (P≤0.07). However, neither HS nor IGF1 had any effect on caspase activity. HS also decreased (P<0.05) GV oocyte mitochondrial activity and developmental competence to the blastocyst stage. These deleterious effects of HS were alleviated (P<0.05) by 12.5ng mL-1 IGF1. This concentration of IGF1 did not affect cleavage rate, the percentage of TUNEL-positive blastomeres and total blastocyst cell number regardless of temperature. In conclusion, exposure of GV oocytes to HS triggered the apoptotic cascade and compromised oocyte developmental competence. Physiological concentrations of IGF1 had a beneficial effect on heat-shocked GV oocytes. |
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Role of insulin-like growth factor 1 on cross-bred Bos indicus cattle germinal vesicle oocytes exposed to heat shockapoptosisdevelopmentgrowth factorIVF.Germinal vesicle (GV) oocytes are susceptible to heat stress. However, neither the cellular mechanisms triggered by elevated temperature nor the thermoprotective effects of insulin-like growth factor (IGF) on GV oocytes are completely understood. Therefore, a series of experiments was conducted to determine the direct effects of IGF1 (0, 12.5, 25, 50 and 100ng mL-1) on heat-treated GV oocytes. Butyrolactone-arrested GV oocytes were cultured at 38.5°C (control) or 41°C (heat shock; HS) for 14h in the presence of different concentrations of IGF1. Exposure of GV oocytes to 41°C increased (P<0.05) the number of terminal deoxyribonucleotidyl transferase-mediated fluorescein-dUTP nick end-labelling (TUNEL)-positive oocytes. At concentrations of 12.5 and 25ng mL-1, IGF1 tended to minimise these negative effect of HS (P≤0.07). However, neither HS nor IGF1 had any effect on caspase activity. HS also decreased (P<0.05) GV oocyte mitochondrial activity and developmental competence to the blastocyst stage. These deleterious effects of HS were alleviated (P<0.05) by 12.5ng mL-1 IGF1. This concentration of IGF1 did not affect cleavage rate, the percentage of TUNEL-positive blastomeres and total blastocyst cell number regardless of temperature. In conclusion, exposure of GV oocytes to HS triggered the apoptotic cascade and compromised oocyte developmental competence. Physiological concentrations of IGF1 had a beneficial effect on heat-shocked GV oocytes.Department of Pharmacology Institute of Biosciences São Paulo State University (UNESP), Botucatu, SP, Rubião Júnior, S/NDepartament of Biological Sciences Federal University of Saõ Paulo, 275 Prof. Artur Riedel St.Departament of Animal Reproduction University of Saõ Paulo, 87 Prof. Dr. Orlando Marques de Paiva Av.Department of Pharmacology Institute of Biosciences São Paulo State University (UNESP), Botucatu, SP, Rubião Júnior, S/NUniversidade Estadual Paulista (Unesp)Federal University of Saõ PauloUniversity of Saõ PauloLima, Rafaela S. [UNESP]Risolia, Pedro H. B. [UNESP]Ispada, JéssicaAssumpção, Mayra E. O. A.Visintin, José A.Orlandi, CássiaPaula-Lopes, Fabíola F. [UNESP]2018-12-11T17:12:49Z2018-12-11T17:12:49Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1405-1414http://dx.doi.org/10.1071/RD15514Reproduction, Fertility and Development, v. 29, n. 7, p. 1405-1414, 2017.1448-59901031-3613http://hdl.handle.net/11449/17478010.1071/RD155142-s2.0-85021049024Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproduction, Fertility and Development0,681info:eu-repo/semantics/openAccess2021-10-23T09:41:46Zoai:repositorio.unesp.br:11449/174780Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:56:00.348197Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Role of insulin-like growth factor 1 on cross-bred Bos indicus cattle germinal vesicle oocytes exposed to heat shock |
title |
Role of insulin-like growth factor 1 on cross-bred Bos indicus cattle germinal vesicle oocytes exposed to heat shock |
spellingShingle |
Role of insulin-like growth factor 1 on cross-bred Bos indicus cattle germinal vesicle oocytes exposed to heat shock Lima, Rafaela S. [UNESP] apoptosis development growth factor IVF. |
title_short |
Role of insulin-like growth factor 1 on cross-bred Bos indicus cattle germinal vesicle oocytes exposed to heat shock |
title_full |
Role of insulin-like growth factor 1 on cross-bred Bos indicus cattle germinal vesicle oocytes exposed to heat shock |
title_fullStr |
Role of insulin-like growth factor 1 on cross-bred Bos indicus cattle germinal vesicle oocytes exposed to heat shock |
title_full_unstemmed |
Role of insulin-like growth factor 1 on cross-bred Bos indicus cattle germinal vesicle oocytes exposed to heat shock |
title_sort |
Role of insulin-like growth factor 1 on cross-bred Bos indicus cattle germinal vesicle oocytes exposed to heat shock |
author |
Lima, Rafaela S. [UNESP] |
author_facet |
Lima, Rafaela S. [UNESP] Risolia, Pedro H. B. [UNESP] Ispada, Jéssica Assumpção, Mayra E. O. A. Visintin, José A. Orlandi, Cássia Paula-Lopes, Fabíola F. [UNESP] |
author_role |
author |
author2 |
Risolia, Pedro H. B. [UNESP] Ispada, Jéssica Assumpção, Mayra E. O. A. Visintin, José A. Orlandi, Cássia Paula-Lopes, Fabíola F. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Federal University of Saõ Paulo University of Saõ Paulo |
dc.contributor.author.fl_str_mv |
Lima, Rafaela S. [UNESP] Risolia, Pedro H. B. [UNESP] Ispada, Jéssica Assumpção, Mayra E. O. A. Visintin, José A. Orlandi, Cássia Paula-Lopes, Fabíola F. [UNESP] |
dc.subject.por.fl_str_mv |
apoptosis development growth factor IVF. |
topic |
apoptosis development growth factor IVF. |
description |
Germinal vesicle (GV) oocytes are susceptible to heat stress. However, neither the cellular mechanisms triggered by elevated temperature nor the thermoprotective effects of insulin-like growth factor (IGF) on GV oocytes are completely understood. Therefore, a series of experiments was conducted to determine the direct effects of IGF1 (0, 12.5, 25, 50 and 100ng mL-1) on heat-treated GV oocytes. Butyrolactone-arrested GV oocytes were cultured at 38.5°C (control) or 41°C (heat shock; HS) for 14h in the presence of different concentrations of IGF1. Exposure of GV oocytes to 41°C increased (P<0.05) the number of terminal deoxyribonucleotidyl transferase-mediated fluorescein-dUTP nick end-labelling (TUNEL)-positive oocytes. At concentrations of 12.5 and 25ng mL-1, IGF1 tended to minimise these negative effect of HS (P≤0.07). However, neither HS nor IGF1 had any effect on caspase activity. HS also decreased (P<0.05) GV oocyte mitochondrial activity and developmental competence to the blastocyst stage. These deleterious effects of HS were alleviated (P<0.05) by 12.5ng mL-1 IGF1. This concentration of IGF1 did not affect cleavage rate, the percentage of TUNEL-positive blastomeres and total blastocyst cell number regardless of temperature. In conclusion, exposure of GV oocytes to HS triggered the apoptotic cascade and compromised oocyte developmental competence. Physiological concentrations of IGF1 had a beneficial effect on heat-shocked GV oocytes. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:12:49Z 2018-12-11T17:12:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1071/RD15514 Reproduction, Fertility and Development, v. 29, n. 7, p. 1405-1414, 2017. 1448-5990 1031-3613 http://hdl.handle.net/11449/174780 10.1071/RD15514 2-s2.0-85021049024 |
url |
http://dx.doi.org/10.1071/RD15514 http://hdl.handle.net/11449/174780 |
identifier_str_mv |
Reproduction, Fertility and Development, v. 29, n. 7, p. 1405-1414, 2017. 1448-5990 1031-3613 10.1071/RD15514 2-s2.0-85021049024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproduction, Fertility and Development 0,681 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1405-1414 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128438069886976 |