Estado físico do meio de cultura na propagação in vitro de bromeliaceae
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://ojs.c3sl.ufpr.br/ojs2/index.php/agraria/article/view/15532 http://hdl.handle.net/11449/71227 |
Resumo: | The objective of this study was to evaluate the effect of the physical state of the culture medium on the behavior in vitro of the bromeliaceas Neoregelia cruenta, Tillandsia stricta, Vriesea gigantea, V. guttata e V. incun/ata. Micropropagated shoots had been cultivated in culture medium MS, with 30 g sucrose, 2,5 mg dm-3 BAP, and 0,5 mg dm-3 ANA, establishing the treatments: T1- half-solid with 7 g agar; T2- static liquid; T3- liquid under-agitation of 90 rpm; and T4-static liquid with bridge of paper filter. After 30 days, the use of static way of liquid culture presented better results in relation of proliferative average rate in all the species (9.4 shoots in N. cruenta, 5.6 in T. stricta, 11.5 in V. gigantea, 9.2 in V. guttata and 3.9 in V. incurvata). In relation the average height of the shoots, was distinguished for N. cruenta the liquid under-agitation (2.83 cm), T. stricta and V. incurvata the semisolid (1.76 cm and 2.02 cm, respectively) and V. gigantea and V. guttata the static liquid (0.61 cm and 1.48 cm, respectively). The use of medium MS static liquid was more suitable for in vitro culture of bromeliads species studied. |
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Estado físico do meio de cultura na propagação in vitro de bromeliaceaeThe physical state of the culture medium on the behavior in vitro of bromeliaceaeBiodiversity conservationMicropropagationOrnamental bromeliadsThe objective of this study was to evaluate the effect of the physical state of the culture medium on the behavior in vitro of the bromeliaceas Neoregelia cruenta, Tillandsia stricta, Vriesea gigantea, V. guttata e V. incun/ata. Micropropagated shoots had been cultivated in culture medium MS, with 30 g sucrose, 2,5 mg dm-3 BAP, and 0,5 mg dm-3 ANA, establishing the treatments: T1- half-solid with 7 g agar; T2- static liquid; T3- liquid under-agitation of 90 rpm; and T4-static liquid with bridge of paper filter. After 30 days, the use of static way of liquid culture presented better results in relation of proliferative average rate in all the species (9.4 shoots in N. cruenta, 5.6 in T. stricta, 11.5 in V. gigantea, 9.2 in V. guttata and 3.9 in V. incurvata). In relation the average height of the shoots, was distinguished for N. cruenta the liquid under-agitation (2.83 cm), T. stricta and V. incurvata the semisolid (1.76 cm and 2.02 cm, respectively) and V. gigantea and V. guttata the static liquid (0.61 cm and 1.48 cm, respectively). The use of medium MS static liquid was more suitable for in vitro culture of bromeliads species studied.Universidade Regional de Blumenau Laboratório de Biotecnologia e Micropropagaçâo Vegetal Universidasde Regional de Blumenau (FURB), Blumenau, Santa CatarinaUniversidade Estadual Paulista Júlio de Mesquita Filho Laboratório de Micropropagaçâo Universidade Estadual de Paulista Júlio de Mesquita Filho'(UNESP), Botucatu, São PauloUniversidade Regional de Blumenau Laboratório de Biotecnologia Micropropagaçâo Vegetal, DCN/T-123 Universidade Regional de Blumenau(FURB), Rua Antônio Haffner, 455, CEP: 89.036-640, Blumenau, Santa CatarinaUniversidade Estadual Paulista Júlio de Mesquita Filho Laboratório de Micropropagaçâo Universidade Estadual de Paulista Júlio de Mesquita Filho'(UNESP), Botucatu, São PauloUniversidasde Regional de Blumenau (FURB)Universidade Estadual Paulista (Unesp)Universidade Federal de Uberlândia (UFU)Mengarda, Liana Hilda GolinPovoas, LucianoDebiasi, Clayton [UNESP]Pescador, Rosete2014-05-27T11:24:02Z2014-05-27T11:24:02Z2009-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article469-474application/pdfhttp://ojs.c3sl.ufpr.br/ojs2/index.php/agraria/article/view/15532Scientia Agraria, v. 10, n. 6, p. 469-474, 2009.1519-11251983-2443http://hdl.handle.net/11449/712272-s2.0-848832918682-s2.0-84883291868.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporScientia Agraria0,1670,167info:eu-repo/semantics/openAccess2023-12-31T06:14:59Zoai:repositorio.unesp.br:11449/71227Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:44:37.591869Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Estado físico do meio de cultura na propagação in vitro de bromeliaceae The physical state of the culture medium on the behavior in vitro of bromeliaceae |
title |
Estado físico do meio de cultura na propagação in vitro de bromeliaceae |
spellingShingle |
Estado físico do meio de cultura na propagação in vitro de bromeliaceae Mengarda, Liana Hilda Golin Biodiversity conservation Micropropagation Ornamental bromeliads |
title_short |
Estado físico do meio de cultura na propagação in vitro de bromeliaceae |
title_full |
Estado físico do meio de cultura na propagação in vitro de bromeliaceae |
title_fullStr |
Estado físico do meio de cultura na propagação in vitro de bromeliaceae |
title_full_unstemmed |
Estado físico do meio de cultura na propagação in vitro de bromeliaceae |
title_sort |
Estado físico do meio de cultura na propagação in vitro de bromeliaceae |
author |
Mengarda, Liana Hilda Golin |
author_facet |
Mengarda, Liana Hilda Golin Povoas, Luciano Debiasi, Clayton [UNESP] Pescador, Rosete |
author_role |
author |
author2 |
Povoas, Luciano Debiasi, Clayton [UNESP] Pescador, Rosete |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidasde Regional de Blumenau (FURB) Universidade Estadual Paulista (Unesp) Universidade Federal de Uberlândia (UFU) |
dc.contributor.author.fl_str_mv |
Mengarda, Liana Hilda Golin Povoas, Luciano Debiasi, Clayton [UNESP] Pescador, Rosete |
dc.subject.por.fl_str_mv |
Biodiversity conservation Micropropagation Ornamental bromeliads |
topic |
Biodiversity conservation Micropropagation Ornamental bromeliads |
description |
The objective of this study was to evaluate the effect of the physical state of the culture medium on the behavior in vitro of the bromeliaceas Neoregelia cruenta, Tillandsia stricta, Vriesea gigantea, V. guttata e V. incun/ata. Micropropagated shoots had been cultivated in culture medium MS, with 30 g sucrose, 2,5 mg dm-3 BAP, and 0,5 mg dm-3 ANA, establishing the treatments: T1- half-solid with 7 g agar; T2- static liquid; T3- liquid under-agitation of 90 rpm; and T4-static liquid with bridge of paper filter. After 30 days, the use of static way of liquid culture presented better results in relation of proliferative average rate in all the species (9.4 shoots in N. cruenta, 5.6 in T. stricta, 11.5 in V. gigantea, 9.2 in V. guttata and 3.9 in V. incurvata). In relation the average height of the shoots, was distinguished for N. cruenta the liquid under-agitation (2.83 cm), T. stricta and V. incurvata the semisolid (1.76 cm and 2.02 cm, respectively) and V. gigantea and V. guttata the static liquid (0.61 cm and 1.48 cm, respectively). The use of medium MS static liquid was more suitable for in vitro culture of bromeliads species studied. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-11-01 2014-05-27T11:24:02Z 2014-05-27T11:24:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://ojs.c3sl.ufpr.br/ojs2/index.php/agraria/article/view/15532 Scientia Agraria, v. 10, n. 6, p. 469-474, 2009. 1519-1125 1983-2443 http://hdl.handle.net/11449/71227 2-s2.0-84883291868 2-s2.0-84883291868.pdf |
url |
http://ojs.c3sl.ufpr.br/ojs2/index.php/agraria/article/view/15532 http://hdl.handle.net/11449/71227 |
identifier_str_mv |
Scientia Agraria, v. 10, n. 6, p. 469-474, 2009. 1519-1125 1983-2443 2-s2.0-84883291868 2-s2.0-84883291868.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Scientia Agraria 0,167 0,167 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
469-474 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129352967127040 |