Genotipagem do locus ABO (9q34.1) em doadores de sangue da região noroeste do estado de São Paulo
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-84842001000100003 http://hdl.handle.net/11449/66692 |
Resumo: | The advances of molecular genetics enabled us to understand the molecular basis of the ABO locus. Considering Us importance as a genetic marker and its applications, the aim of this study was to verify the distribution of the ABO genotypes in a Brazilian population from the Northwest region of the Sào Paulo Stale, Brazil. The genomic DNA was extracted from three hundred and twenty four healthy Brazilian blood donors (O ] 50; A 118; B 32 and AB 24) and analyzed by PCR amplification followed by restriction enzyme digestion. Fourteen genotypes were identified and the relative frequencies of the O , O , O , A and B genes ivere estimated at 44.6%, 16.9%, 4.1%, 25.3% and 9.1%, respectively. Tloese results demonstrate that the ABO locus presents a high polymorphism as revealed by molecular analysis. |
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Genotipagem do locus ABO (9q34.1) em doadores de sangue da região noroeste do estado de São PauloLocus ABO (9q 34.1) genotyping in blood donors in the state of Sao Paulo, BrazilABO genesABO genotypesBlood donorsThe advances of molecular genetics enabled us to understand the molecular basis of the ABO locus. Considering Us importance as a genetic marker and its applications, the aim of this study was to verify the distribution of the ABO genotypes in a Brazilian population from the Northwest region of the Sào Paulo Stale, Brazil. The genomic DNA was extracted from three hundred and twenty four healthy Brazilian blood donors (O ] 50; A 118; B 32 and AB 24) and analyzed by PCR amplification followed by restriction enzyme digestion. Fourteen genotypes were identified and the relative frequencies of the O , O , O , A and B genes ivere estimated at 44.6%, 16.9%, 4.1%, 25.3% and 9.1%, respectively. Tloese results demonstrate that the ABO locus presents a high polymorphism as revealed by molecular analysis.Departamento de Biologia Molecular Faculdade de Medicina de Sào José do Rio Preto, SPLaboralório de Imunogenélica Molecular Hemocenlro de Sào José do Rio Prelo, SPDepartamento de Biologia Ibilce-Unesp - Sào José do Rio Prelo, SPDepartamento de Anälises Clínicas Faculdade de Ciências Farmacêulicas Unesp, Araraquara, SPDepartamento de Biologia Molecular Faculdade de Medicina de Sào José do Rio Prêta, Avenida Brigadeiro Faria Lima, 5416, 15090-000 Sào josé do Rio Preto, SPDepartamento de Biologia Ibilce-Unesp - Sào José do Rio Prelo, SPDepartamento de Anälises Clínicas Faculdade de Ciências Farmacêulicas Unesp, Araraquara, SPFaculdade de Medicina de São José do Rio Preto (FAMERP)Universidade Estadual Paulista (Unesp)Mattos, Luiz C.Sancbez, Fábio E.Cintra, Juliana R.Salles, Andréa B. C. F.Bonini-Domingos, Cláudia R. [UNESP]Moreira, Haroldo Wilson [UNESP]2014-05-27T11:20:21Z2014-05-27T11:20:21Z2001-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15-22application/pdfhttp://dx.doi.org/10.1590/S1516-84842001000100003Revista Brasileira de Hematologia e Hemoterapia, v. 23, n. 1, p. 15-22, 2001.1516-8484http://hdl.handle.net/11449/6669210.1590/S1516-84842001000100003S1516-848420010001000032-s2.0-301444331602-s2.0-30144433160.pdf32794280661767190000-0002-4603-9467Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Hematologia e Hemoterapia0,335info:eu-repo/semantics/openAccess2024-06-21T15:18:34Zoai:repositorio.unesp.br:11449/66692Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:59:51.124353Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genotipagem do locus ABO (9q34.1) em doadores de sangue da região noroeste do estado de São Paulo Locus ABO (9q 34.1) genotyping in blood donors in the state of Sao Paulo, Brazil |
title |
Genotipagem do locus ABO (9q34.1) em doadores de sangue da região noroeste do estado de São Paulo |
spellingShingle |
Genotipagem do locus ABO (9q34.1) em doadores de sangue da região noroeste do estado de São Paulo Mattos, Luiz C. ABO genes ABO genotypes Blood donors |
title_short |
Genotipagem do locus ABO (9q34.1) em doadores de sangue da região noroeste do estado de São Paulo |
title_full |
Genotipagem do locus ABO (9q34.1) em doadores de sangue da região noroeste do estado de São Paulo |
title_fullStr |
Genotipagem do locus ABO (9q34.1) em doadores de sangue da região noroeste do estado de São Paulo |
title_full_unstemmed |
Genotipagem do locus ABO (9q34.1) em doadores de sangue da região noroeste do estado de São Paulo |
title_sort |
Genotipagem do locus ABO (9q34.1) em doadores de sangue da região noroeste do estado de São Paulo |
author |
Mattos, Luiz C. |
author_facet |
Mattos, Luiz C. Sancbez, Fábio E. Cintra, Juliana R. Salles, Andréa B. C. F. Bonini-Domingos, Cláudia R. [UNESP] Moreira, Haroldo Wilson [UNESP] |
author_role |
author |
author2 |
Sancbez, Fábio E. Cintra, Juliana R. Salles, Andréa B. C. F. Bonini-Domingos, Cláudia R. [UNESP] Moreira, Haroldo Wilson [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Faculdade de Medicina de São José do Rio Preto (FAMERP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Mattos, Luiz C. Sancbez, Fábio E. Cintra, Juliana R. Salles, Andréa B. C. F. Bonini-Domingos, Cláudia R. [UNESP] Moreira, Haroldo Wilson [UNESP] |
dc.subject.por.fl_str_mv |
ABO genes ABO genotypes Blood donors |
topic |
ABO genes ABO genotypes Blood donors |
description |
The advances of molecular genetics enabled us to understand the molecular basis of the ABO locus. Considering Us importance as a genetic marker and its applications, the aim of this study was to verify the distribution of the ABO genotypes in a Brazilian population from the Northwest region of the Sào Paulo Stale, Brazil. The genomic DNA was extracted from three hundred and twenty four healthy Brazilian blood donors (O ] 50; A 118; B 32 and AB 24) and analyzed by PCR amplification followed by restriction enzyme digestion. Fourteen genotypes were identified and the relative frequencies of the O , O , O , A and B genes ivere estimated at 44.6%, 16.9%, 4.1%, 25.3% and 9.1%, respectively. Tloese results demonstrate that the ABO locus presents a high polymorphism as revealed by molecular analysis. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-12-01 2014-05-27T11:20:21Z 2014-05-27T11:20:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-84842001000100003 Revista Brasileira de Hematologia e Hemoterapia, v. 23, n. 1, p. 15-22, 2001. 1516-8484 http://hdl.handle.net/11449/66692 10.1590/S1516-84842001000100003 S1516-84842001000100003 2-s2.0-30144433160 2-s2.0-30144433160.pdf 3279428066176719 0000-0002-4603-9467 |
url |
http://dx.doi.org/10.1590/S1516-84842001000100003 http://hdl.handle.net/11449/66692 |
identifier_str_mv |
Revista Brasileira de Hematologia e Hemoterapia, v. 23, n. 1, p. 15-22, 2001. 1516-8484 10.1590/S1516-84842001000100003 S1516-84842001000100003 2-s2.0-30144433160 2-s2.0-30144433160.pdf 3279428066176719 0000-0002-4603-9467 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista Brasileira de Hematologia e Hemoterapia 0,335 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
15-22 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128590420639744 |