A comparison of novel inactivation methods for production of a vaccine against Streptococcus agalactiae in Nile tilapia Oreochromis niloticus

Detalhes bibliográficos
Autor(a) principal: Ramos-Espinoza, Fernando Carlos [UNESP]
Data de Publicação: 2020
Outros Autores: Cueva-Quiroz, Victor Alexander [UNESP], Yunis-Aguinaga, Jefferson [UNESP], de Moraes, Julieta Rodini Engrácia [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.aquaculture.2020.735484
http://hdl.handle.net/11449/200552
Resumo: Disease prevention and control are crucial for the development of intensive commercial aquaculture. For some fish species, several vaccines are available to contain the effects of pathogens. However, in Nile tilapia there are just a few vaccines available. The current study compared the efficacy of three inactivated vaccines against Streptococcus agalactiae; in Nile tilapia. Formaldehyde, hydrogen peroxide and pH manipulation were used to inactivate the bacteria. Nile tilapia fingerlings were distributed into six experimental groups in duplicate: T1: naïve group; T2: sham group (PBS-injected, non-challenged); T3: PBS-injected control group (PBS-injected, challenge-infected); T4: vaccinated with hydrogen peroxide-inactivated vaccine (vaccinated, challenge-infected); T5: vaccinated with formaldehyde-inactivated vaccine (vaccinated, challenge-infected); T6: vaccinated by pH manipulation (vaccinated, challenge-infected). Twenty-eight days after vaccination, groups T3, T4, T5 and T6 were challenged intraperitoneally with 0.1 mL of the bacteria and monitored for 21 days. Additionally, replicates of non-challenged fish were used for determination of total serum protein and anti-Streptococcus agalactiae IgM antibodies. The percentage of survival of the groups T1, T2, T3, T4, T5 and T6 were 100%, 100%, 62.5%, 80%, 75% and 85%, respectively. The group vaccinated by pH manipulation presented slight higher survival when compared to the control group (P > .05). However, there were no differences in survival between vaccinated groups. The relative percentage survival (RPS) in groups T4, T5 and T6 were 46.6%, 33.3% and 60%, respectively. No differences were observed for total serum proteins and anti-Streptococcus. agalactiae IgM antibodies. The results observed in this study indicate equivalent moderate vaccine efficacy between alternative inactivated (hydrogen peroxide and pH), and conventional formaldehyde vaccines, with slight higher values for the pH manipulated vaccine, which suggests that future studies should be performed for each alternative method in order to improve their performance.
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spelling A comparison of novel inactivation methods for production of a vaccine against Streptococcus agalactiae in Nile tilapia Oreochromis niloticusAquacultureBacterial inactivationFormaldehydeHydrogen peroxideVaccine efficacyDisease prevention and control are crucial for the development of intensive commercial aquaculture. For some fish species, several vaccines are available to contain the effects of pathogens. However, in Nile tilapia there are just a few vaccines available. The current study compared the efficacy of three inactivated vaccines against Streptococcus agalactiae; in Nile tilapia. Formaldehyde, hydrogen peroxide and pH manipulation were used to inactivate the bacteria. Nile tilapia fingerlings were distributed into six experimental groups in duplicate: T1: naïve group; T2: sham group (PBS-injected, non-challenged); T3: PBS-injected control group (PBS-injected, challenge-infected); T4: vaccinated with hydrogen peroxide-inactivated vaccine (vaccinated, challenge-infected); T5: vaccinated with formaldehyde-inactivated vaccine (vaccinated, challenge-infected); T6: vaccinated by pH manipulation (vaccinated, challenge-infected). Twenty-eight days after vaccination, groups T3, T4, T5 and T6 were challenged intraperitoneally with 0.1 mL of the bacteria and monitored for 21 days. Additionally, replicates of non-challenged fish were used for determination of total serum protein and anti-Streptococcus agalactiae IgM antibodies. The percentage of survival of the groups T1, T2, T3, T4, T5 and T6 were 100%, 100%, 62.5%, 80%, 75% and 85%, respectively. The group vaccinated by pH manipulation presented slight higher survival when compared to the control group (P > .05). However, there were no differences in survival between vaccinated groups. The relative percentage survival (RPS) in groups T4, T5 and T6 were 46.6%, 33.3% and 60%, respectively. No differences were observed for total serum proteins and anti-Streptococcus. agalactiae IgM antibodies. The results observed in this study indicate equivalent moderate vaccine efficacy between alternative inactivated (hydrogen peroxide and pH), and conventional formaldehyde vaccines, with slight higher values for the pH manipulated vaccine, which suggests that future studies should be performed for each alternative method in order to improve their performance.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Aquaculture Center of Unesp (Caunesp) Sao Paulo State University (Unesp) Via Prof. Paulo Donato Castellane, km 05, 14Aquatic Pathobiology Laboratory Sea Institute of Peru (IMARPE), Esquina Gamarra y GeneralValle s/n, ChucuitoDepartment of Veterinary Pathology School of Agricultural and Veterinary Sciences (FCAV), Unesp. Via Prof. Paulo Donato Castellane, km 05, 14Aquaculture Center of Unesp (Caunesp) Sao Paulo State University (Unesp) Via Prof. Paulo Donato Castellane, km 05, 14Universidade Estadual Paulista (Unesp)(IMARPE)Ramos-Espinoza, Fernando Carlos [UNESP]Cueva-Quiroz, Victor Alexander [UNESP]Yunis-Aguinaga, Jefferson [UNESP]de Moraes, Julieta Rodini Engrácia [UNESP]2020-12-12T02:09:35Z2020-12-12T02:09:35Z2020-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.aquaculture.2020.735484Aquaculture, v. 528.0044-8486http://hdl.handle.net/11449/20055210.1016/j.aquaculture.2020.7354842-s2.0-85085897060Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquacultureinfo:eu-repo/semantics/openAccess2024-04-09T15:37:12Zoai:repositorio.unesp.br:11449/200552Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:17:03.828707Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A comparison of novel inactivation methods for production of a vaccine against Streptococcus agalactiae in Nile tilapia Oreochromis niloticus
title A comparison of novel inactivation methods for production of a vaccine against Streptococcus agalactiae in Nile tilapia Oreochromis niloticus
spellingShingle A comparison of novel inactivation methods for production of a vaccine against Streptococcus agalactiae in Nile tilapia Oreochromis niloticus
Ramos-Espinoza, Fernando Carlos [UNESP]
Aquaculture
Bacterial inactivation
Formaldehyde
Hydrogen peroxide
Vaccine efficacy
title_short A comparison of novel inactivation methods for production of a vaccine against Streptococcus agalactiae in Nile tilapia Oreochromis niloticus
title_full A comparison of novel inactivation methods for production of a vaccine against Streptococcus agalactiae in Nile tilapia Oreochromis niloticus
title_fullStr A comparison of novel inactivation methods for production of a vaccine against Streptococcus agalactiae in Nile tilapia Oreochromis niloticus
title_full_unstemmed A comparison of novel inactivation methods for production of a vaccine against Streptococcus agalactiae in Nile tilapia Oreochromis niloticus
title_sort A comparison of novel inactivation methods for production of a vaccine against Streptococcus agalactiae in Nile tilapia Oreochromis niloticus
author Ramos-Espinoza, Fernando Carlos [UNESP]
author_facet Ramos-Espinoza, Fernando Carlos [UNESP]
Cueva-Quiroz, Victor Alexander [UNESP]
Yunis-Aguinaga, Jefferson [UNESP]
de Moraes, Julieta Rodini Engrácia [UNESP]
author_role author
author2 Cueva-Quiroz, Victor Alexander [UNESP]
Yunis-Aguinaga, Jefferson [UNESP]
de Moraes, Julieta Rodini Engrácia [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
(IMARPE)
dc.contributor.author.fl_str_mv Ramos-Espinoza, Fernando Carlos [UNESP]
Cueva-Quiroz, Victor Alexander [UNESP]
Yunis-Aguinaga, Jefferson [UNESP]
de Moraes, Julieta Rodini Engrácia [UNESP]
dc.subject.por.fl_str_mv Aquaculture
Bacterial inactivation
Formaldehyde
Hydrogen peroxide
Vaccine efficacy
topic Aquaculture
Bacterial inactivation
Formaldehyde
Hydrogen peroxide
Vaccine efficacy
description Disease prevention and control are crucial for the development of intensive commercial aquaculture. For some fish species, several vaccines are available to contain the effects of pathogens. However, in Nile tilapia there are just a few vaccines available. The current study compared the efficacy of three inactivated vaccines against Streptococcus agalactiae; in Nile tilapia. Formaldehyde, hydrogen peroxide and pH manipulation were used to inactivate the bacteria. Nile tilapia fingerlings were distributed into six experimental groups in duplicate: T1: naïve group; T2: sham group (PBS-injected, non-challenged); T3: PBS-injected control group (PBS-injected, challenge-infected); T4: vaccinated with hydrogen peroxide-inactivated vaccine (vaccinated, challenge-infected); T5: vaccinated with formaldehyde-inactivated vaccine (vaccinated, challenge-infected); T6: vaccinated by pH manipulation (vaccinated, challenge-infected). Twenty-eight days after vaccination, groups T3, T4, T5 and T6 were challenged intraperitoneally with 0.1 mL of the bacteria and monitored for 21 days. Additionally, replicates of non-challenged fish were used for determination of total serum protein and anti-Streptococcus agalactiae IgM antibodies. The percentage of survival of the groups T1, T2, T3, T4, T5 and T6 were 100%, 100%, 62.5%, 80%, 75% and 85%, respectively. The group vaccinated by pH manipulation presented slight higher survival when compared to the control group (P > .05). However, there were no differences in survival between vaccinated groups. The relative percentage survival (RPS) in groups T4, T5 and T6 were 46.6%, 33.3% and 60%, respectively. No differences were observed for total serum proteins and anti-Streptococcus. agalactiae IgM antibodies. The results observed in this study indicate equivalent moderate vaccine efficacy between alternative inactivated (hydrogen peroxide and pH), and conventional formaldehyde vaccines, with slight higher values for the pH manipulated vaccine, which suggests that future studies should be performed for each alternative method in order to improve their performance.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:09:35Z
2020-12-12T02:09:35Z
2020-11-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.aquaculture.2020.735484
Aquaculture, v. 528.
0044-8486
http://hdl.handle.net/11449/200552
10.1016/j.aquaculture.2020.735484
2-s2.0-85085897060
url http://dx.doi.org/10.1016/j.aquaculture.2020.735484
http://hdl.handle.net/11449/200552
identifier_str_mv Aquaculture, v. 528.
0044-8486
10.1016/j.aquaculture.2020.735484
2-s2.0-85085897060
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Aquaculture
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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