Molecular detection and genetic diversity of Bartonella species in large ruminants and associated ectoparasites from the Brazilian Cerrado
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/tbed.13517 http://hdl.handle.net/11449/200151 |
Resumo: | Currently, five Bartonella species and an expanding number of Candidatus Bartonella species have globally been reported in ruminants. Likewise, different Bartonella genotypes were identified. However, studies relating to ruminant-associated Bartonella in Brazil are scarce. The current study aimed to assess the prevalence and genetic diversity of Bartonella in cattle, buffaloes and associated ectoparasites in Brazil. For this purpose, EDTA-blood samples from 75 cattle and 101 buffaloes were sampled. Additionally, 128 Rhipicephalus microplus and one Amblyomma sculptum ticks collected from cattle, and 197 R. microplus, one A. sculptum and 170 lice (Haematopinus tuberculatus) collected from buffaloes were included. Bartonella DNA was initially screened through an HRM real-time PCR assay targeting the 16S–23S internal transcribed spacer (ITS), and the positive samples were submitted to an additional HRM assay targeting the ssrA gene. The HRM-positive amplicons were sequenced, and the nucleotide identity was assessed by BLASTn. Bartonella spp.-positive DNA samples were analysed by conventional PCR assays targeting the gltA and rpoB genes, and then, the samples were cloned. Finally, the phylogenetic positioning and the genetic diversity of clones were assessed. Overall, 21 of 75 (28%) cattle blood samples and 13 of 126 (10.3%) associated ticks were positive for Bartonella bovis. Out of 101 buffaloes, 95 lice and 188 tick DNA samples, one (1%) buffalo and four (4.2%) lice were positive for Bartonella spp. Conversely, none of the ticks obtained from buffaloes were positive for Bartonella. The Bartonella sequences from buffaloes showed identity ranging from 100% (ITS and gltA) to 94% (ssrA) with B. bovis. In contrast, the Bartonella DNA sequences from lice were identical (100%) to uncultured Bartonella sp. detected in cattle tail louse (Haematopinus quadripertusus) from Israel in all amplified genes. The present study demonstrates the prevalence of new B. bovis genotypes and a cattle lice-associated Bartonella species in large ruminants and their ectoparasites from Brazil. These findings shed light on the distribution and genetic diversity of ruminant- and ectoparasite-related Bartonella in Brazil. |
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Molecular detection and genetic diversity of Bartonella species in large ruminants and associated ectoparasites from the Brazilian Cerradobartonellosisbuffaloescattlegenetic diversityHaematopinus tuberculatusRhipicephalus microplusCurrently, five Bartonella species and an expanding number of Candidatus Bartonella species have globally been reported in ruminants. Likewise, different Bartonella genotypes were identified. However, studies relating to ruminant-associated Bartonella in Brazil are scarce. The current study aimed to assess the prevalence and genetic diversity of Bartonella in cattle, buffaloes and associated ectoparasites in Brazil. For this purpose, EDTA-blood samples from 75 cattle and 101 buffaloes were sampled. Additionally, 128 Rhipicephalus microplus and one Amblyomma sculptum ticks collected from cattle, and 197 R. microplus, one A. sculptum and 170 lice (Haematopinus tuberculatus) collected from buffaloes were included. Bartonella DNA was initially screened through an HRM real-time PCR assay targeting the 16S–23S internal transcribed spacer (ITS), and the positive samples were submitted to an additional HRM assay targeting the ssrA gene. The HRM-positive amplicons were sequenced, and the nucleotide identity was assessed by BLASTn. Bartonella spp.-positive DNA samples were analysed by conventional PCR assays targeting the gltA and rpoB genes, and then, the samples were cloned. Finally, the phylogenetic positioning and the genetic diversity of clones were assessed. Overall, 21 of 75 (28%) cattle blood samples and 13 of 126 (10.3%) associated ticks were positive for Bartonella bovis. Out of 101 buffaloes, 95 lice and 188 tick DNA samples, one (1%) buffalo and four (4.2%) lice were positive for Bartonella spp. Conversely, none of the ticks obtained from buffaloes were positive for Bartonella. The Bartonella sequences from buffaloes showed identity ranging from 100% (ITS and gltA) to 94% (ssrA) with B. bovis. In contrast, the Bartonella DNA sequences from lice were identical (100%) to uncultured Bartonella sp. detected in cattle tail louse (Haematopinus quadripertusus) from Israel in all amplified genes. The present study demonstrates the prevalence of new B. bovis genotypes and a cattle lice-associated Bartonella species in large ruminants and their ectoparasites from Brazil. These findings shed light on the distribution and genetic diversity of ruminant- and ectoparasite-related Bartonella in Brazil.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do SulGraduate Program of Agricultural and Livestock Microbiology Faculty of Agrarian and Veterinary Sciences (FCAV/UNESP)Department of Veterinary Pathology Faculty of Agrarian and Veterinary Sciences (FCAV/UNESP)Koret School of Veterinary Medicine The Robert H. Smith Faculty of Agriculture Food and Environment The Hebrew University of JerusalemDom Bosco Catholic UniversityGraduate Program of Natural Resources Federal University of Mato Grosso do SulGraduate Program of Agricultural and Livestock Microbiology Faculty of Agrarian and Veterinary Sciences (FCAV/UNESP)Department of Veterinary Pathology Faculty of Agrarian and Veterinary Sciences (FCAV/UNESP)FAPESP: 2018/02753-0CNPq: 302420/2017-7Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul: 59/300.187/2016Universidade Estadual Paulista (Unesp)The Hebrew University of JerusalemDom Bosco Catholic UniversityFederal University of Mato Grosso do SulGonçalves, Luiz Ricardo [UNESP]Harrus, ShimonGutiérrez, RicardoHerrera, Heitor Miragliade Souza Ramos, Inalda Angélica [UNESP]Porfírio, Grasiela Edith de OliveiraNachum-Biala, Yaaritde Sousa, Keyla Carstens Marquesda Silva, Thiago Merighi Vieira [UNESP]Campos, João Bosco VilelaLemos, Wagner [UNESP]Moraes Barros-Battesti, Darci [UNESP]Machado, Rosangela Zacarias [UNESP]André, Marcos Rogério [UNESP]2020-12-12T01:59:03Z2020-12-12T01:59:03Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1888-1897http://dx.doi.org/10.1111/tbed.13517Transboundary and Emerging Diseases, v. 67, n. 5, p. 1888-1897, 2020.1865-16821865-1674http://hdl.handle.net/11449/20015110.1111/tbed.135172-s2.0-85081409309Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTransboundary and Emerging Diseasesinfo:eu-repo/semantics/openAccess2021-10-23T12:24:03Zoai:repositorio.unesp.br:11449/200151Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T12:24:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular detection and genetic diversity of Bartonella species in large ruminants and associated ectoparasites from the Brazilian Cerrado |
title |
Molecular detection and genetic diversity of Bartonella species in large ruminants and associated ectoparasites from the Brazilian Cerrado |
spellingShingle |
Molecular detection and genetic diversity of Bartonella species in large ruminants and associated ectoparasites from the Brazilian Cerrado Gonçalves, Luiz Ricardo [UNESP] bartonellosis buffaloes cattle genetic diversity Haematopinus tuberculatus Rhipicephalus microplus |
title_short |
Molecular detection and genetic diversity of Bartonella species in large ruminants and associated ectoparasites from the Brazilian Cerrado |
title_full |
Molecular detection and genetic diversity of Bartonella species in large ruminants and associated ectoparasites from the Brazilian Cerrado |
title_fullStr |
Molecular detection and genetic diversity of Bartonella species in large ruminants and associated ectoparasites from the Brazilian Cerrado |
title_full_unstemmed |
Molecular detection and genetic diversity of Bartonella species in large ruminants and associated ectoparasites from the Brazilian Cerrado |
title_sort |
Molecular detection and genetic diversity of Bartonella species in large ruminants and associated ectoparasites from the Brazilian Cerrado |
author |
Gonçalves, Luiz Ricardo [UNESP] |
author_facet |
Gonçalves, Luiz Ricardo [UNESP] Harrus, Shimon Gutiérrez, Ricardo Herrera, Heitor Miraglia de Souza Ramos, Inalda Angélica [UNESP] Porfírio, Grasiela Edith de Oliveira Nachum-Biala, Yaarit de Sousa, Keyla Carstens Marques da Silva, Thiago Merighi Vieira [UNESP] Campos, João Bosco Vilela Lemos, Wagner [UNESP] Moraes Barros-Battesti, Darci [UNESP] Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
author_role |
author |
author2 |
Harrus, Shimon Gutiérrez, Ricardo Herrera, Heitor Miraglia de Souza Ramos, Inalda Angélica [UNESP] Porfírio, Grasiela Edith de Oliveira Nachum-Biala, Yaarit de Sousa, Keyla Carstens Marques da Silva, Thiago Merighi Vieira [UNESP] Campos, João Bosco Vilela Lemos, Wagner [UNESP] Moraes Barros-Battesti, Darci [UNESP] Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) The Hebrew University of Jerusalem Dom Bosco Catholic University Federal University of Mato Grosso do Sul |
dc.contributor.author.fl_str_mv |
Gonçalves, Luiz Ricardo [UNESP] Harrus, Shimon Gutiérrez, Ricardo Herrera, Heitor Miraglia de Souza Ramos, Inalda Angélica [UNESP] Porfírio, Grasiela Edith de Oliveira Nachum-Biala, Yaarit de Sousa, Keyla Carstens Marques da Silva, Thiago Merighi Vieira [UNESP] Campos, João Bosco Vilela Lemos, Wagner [UNESP] Moraes Barros-Battesti, Darci [UNESP] Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
dc.subject.por.fl_str_mv |
bartonellosis buffaloes cattle genetic diversity Haematopinus tuberculatus Rhipicephalus microplus |
topic |
bartonellosis buffaloes cattle genetic diversity Haematopinus tuberculatus Rhipicephalus microplus |
description |
Currently, five Bartonella species and an expanding number of Candidatus Bartonella species have globally been reported in ruminants. Likewise, different Bartonella genotypes were identified. However, studies relating to ruminant-associated Bartonella in Brazil are scarce. The current study aimed to assess the prevalence and genetic diversity of Bartonella in cattle, buffaloes and associated ectoparasites in Brazil. For this purpose, EDTA-blood samples from 75 cattle and 101 buffaloes were sampled. Additionally, 128 Rhipicephalus microplus and one Amblyomma sculptum ticks collected from cattle, and 197 R. microplus, one A. sculptum and 170 lice (Haematopinus tuberculatus) collected from buffaloes were included. Bartonella DNA was initially screened through an HRM real-time PCR assay targeting the 16S–23S internal transcribed spacer (ITS), and the positive samples were submitted to an additional HRM assay targeting the ssrA gene. The HRM-positive amplicons were sequenced, and the nucleotide identity was assessed by BLASTn. Bartonella spp.-positive DNA samples were analysed by conventional PCR assays targeting the gltA and rpoB genes, and then, the samples were cloned. Finally, the phylogenetic positioning and the genetic diversity of clones were assessed. Overall, 21 of 75 (28%) cattle blood samples and 13 of 126 (10.3%) associated ticks were positive for Bartonella bovis. Out of 101 buffaloes, 95 lice and 188 tick DNA samples, one (1%) buffalo and four (4.2%) lice were positive for Bartonella spp. Conversely, none of the ticks obtained from buffaloes were positive for Bartonella. The Bartonella sequences from buffaloes showed identity ranging from 100% (ITS and gltA) to 94% (ssrA) with B. bovis. In contrast, the Bartonella DNA sequences from lice were identical (100%) to uncultured Bartonella sp. detected in cattle tail louse (Haematopinus quadripertusus) from Israel in all amplified genes. The present study demonstrates the prevalence of new B. bovis genotypes and a cattle lice-associated Bartonella species in large ruminants and their ectoparasites from Brazil. These findings shed light on the distribution and genetic diversity of ruminant- and ectoparasite-related Bartonella in Brazil. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:59:03Z 2020-12-12T01:59:03Z 2020-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/tbed.13517 Transboundary and Emerging Diseases, v. 67, n. 5, p. 1888-1897, 2020. 1865-1682 1865-1674 http://hdl.handle.net/11449/200151 10.1111/tbed.13517 2-s2.0-85081409309 |
url |
http://dx.doi.org/10.1111/tbed.13517 http://hdl.handle.net/11449/200151 |
identifier_str_mv |
Transboundary and Emerging Diseases, v. 67, n. 5, p. 1888-1897, 2020. 1865-1682 1865-1674 10.1111/tbed.13517 2-s2.0-85081409309 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Transboundary and Emerging Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1888-1897 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965186418802688 |