Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5897/AJB10.793 http://hdl.handle.net/11449/7371 |
Resumo: | Previously, we have demonstrated that treatment of experimental diabetes with a decoction of Bauhinia forficata leaves is beneficial. In this study, we prepared a two-fold concentrate of this extract and tested its effects on physiological, biochemical and toxicity markers in streptozotocin-diabetic rats. Dried and ground leaves were extracted with warm 70% hydroethanol and the filtrate concentrated by evaporation at 50 degrees C. This solution was mixed with colloidal silicon dioxide (Tixosil-333 (R)) and dried in a spouted bed (BfT). Rats were treated with water, insulin and Tixosil particles at low or high doses, alone or coated with dried BfT. Animals were periodically weighed and monitored for water and food intake; urinary volume, glucose, urea and protein; blood glucose, serum lipids, liver toxicity markers transaminase and phosphatase and masses of adipose tissue and skeletal muscle. Insulin treatment gave best rat growth and lowest values for all other markers. No other treatment affected any diabetic marker, but the enzyme activities were changed by diabetes and BfT. Thus, BfT toxicity could arise from secondary products of plant constituents or Tixosil interaction. Therefore, BfT prepared in the spouted bed as described, is unsuitable for treatment of diabetes, which implies that the method of preparation of any medicine is critical for its efficacy and toxicity. |
id |
UNSP_138b446f9f0519efc03c85e885d626dd |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/7371 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in ratsBauhinia forficataenzymesphysiological variablesplasma glucoseserum lipidsserum toxicity markersspouted bed dryingPreviously, we have demonstrated that treatment of experimental diabetes with a decoction of Bauhinia forficata leaves is beneficial. In this study, we prepared a two-fold concentrate of this extract and tested its effects on physiological, biochemical and toxicity markers in streptozotocin-diabetic rats. Dried and ground leaves were extracted with warm 70% hydroethanol and the filtrate concentrated by evaporation at 50 degrees C. This solution was mixed with colloidal silicon dioxide (Tixosil-333 (R)) and dried in a spouted bed (BfT). Rats were treated with water, insulin and Tixosil particles at low or high doses, alone or coated with dried BfT. Animals were periodically weighed and monitored for water and food intake; urinary volume, glucose, urea and protein; blood glucose, serum lipids, liver toxicity markers transaminase and phosphatase and masses of adipose tissue and skeletal muscle. Insulin treatment gave best rat growth and lowest values for all other markers. No other treatment affected any diabetic marker, but the enzyme activities were changed by diabetes and BfT. Thus, BfT toxicity could arise from secondary products of plant constituents or Tixosil interaction. Therefore, BfT prepared in the spouted bed as described, is unsuitable for treatment of diabetes, which implies that the method of preparation of any medicine is critical for its efficacy and toxicity.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)PADC-Faculdade de Ciencias Farmaceuticas (FCF)-Araraquara-Universidade Estadual Paulista Julio de Mesquita Filho (UNESP)Univ Estadual Paulista, Dept Anal Clin, Fac Ciencias Farmaceut, UNESP, São Paulo, BrazilUniv São Paulo, Lab Proc Farmaceut, Fac Ciencias Farmaceut Ribeirao Preto, USP, BR-05508 São Paulo, BrazilUniv Estadual Paulista, Dept Anal Clin, Fac Ciencias Farmaceut, UNESP, São Paulo, BrazilAcademic JournalsUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Pepato, Maria Teresa [UNESP]Conceicao, C. Q. [UNESP]Gutierres, V. O. [UNESP]Vendramini, Regina Célia [UNESP]Souza, C. R. F.Oliveira, W. P.Brunetti, Iguatemy Lourenço [UNESP]2014-05-20T13:24:02Z2014-05-20T13:24:02Z2010-10-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7165-7173application/pdfhttp://dx.doi.org/10.5897/AJB10.793African Journal of Biotechnology. Victoria Island: Academic Journals, v. 9, n. 42, p. 7165-7173, 2010.1684-5315http://hdl.handle.net/11449/737110.5897/AJB10.793WOS:000283197400022WOS000283197400022.pdf7641979287850489Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAfrican Journal of Biotechnologyinfo:eu-repo/semantics/openAccess2024-01-09T06:29:05Zoai:repositorio.unesp.br:11449/7371Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-09T06:29:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats |
title |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats |
spellingShingle |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats Pepato, Maria Teresa [UNESP] Bauhinia forficata enzymes physiological variables plasma glucose serum lipids serum toxicity markers spouted bed drying |
title_short |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats |
title_full |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats |
title_fullStr |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats |
title_full_unstemmed |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats |
title_sort |
Evaluation of the spouted bed dried leaf extract of Bauhinia forficata for the treatment of experimental diabetes in rats |
author |
Pepato, Maria Teresa [UNESP] |
author_facet |
Pepato, Maria Teresa [UNESP] Conceicao, C. Q. [UNESP] Gutierres, V. O. [UNESP] Vendramini, Regina Célia [UNESP] Souza, C. R. F. Oliveira, W. P. Brunetti, Iguatemy Lourenço [UNESP] |
author_role |
author |
author2 |
Conceicao, C. Q. [UNESP] Gutierres, V. O. [UNESP] Vendramini, Regina Célia [UNESP] Souza, C. R. F. Oliveira, W. P. Brunetti, Iguatemy Lourenço [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Pepato, Maria Teresa [UNESP] Conceicao, C. Q. [UNESP] Gutierres, V. O. [UNESP] Vendramini, Regina Célia [UNESP] Souza, C. R. F. Oliveira, W. P. Brunetti, Iguatemy Lourenço [UNESP] |
dc.subject.por.fl_str_mv |
Bauhinia forficata enzymes physiological variables plasma glucose serum lipids serum toxicity markers spouted bed drying |
topic |
Bauhinia forficata enzymes physiological variables plasma glucose serum lipids serum toxicity markers spouted bed drying |
description |
Previously, we have demonstrated that treatment of experimental diabetes with a decoction of Bauhinia forficata leaves is beneficial. In this study, we prepared a two-fold concentrate of this extract and tested its effects on physiological, biochemical and toxicity markers in streptozotocin-diabetic rats. Dried and ground leaves were extracted with warm 70% hydroethanol and the filtrate concentrated by evaporation at 50 degrees C. This solution was mixed with colloidal silicon dioxide (Tixosil-333 (R)) and dried in a spouted bed (BfT). Rats were treated with water, insulin and Tixosil particles at low or high doses, alone or coated with dried BfT. Animals were periodically weighed and monitored for water and food intake; urinary volume, glucose, urea and protein; blood glucose, serum lipids, liver toxicity markers transaminase and phosphatase and masses of adipose tissue and skeletal muscle. Insulin treatment gave best rat growth and lowest values for all other markers. No other treatment affected any diabetic marker, but the enzyme activities were changed by diabetes and BfT. Thus, BfT toxicity could arise from secondary products of plant constituents or Tixosil interaction. Therefore, BfT prepared in the spouted bed as described, is unsuitable for treatment of diabetes, which implies that the method of preparation of any medicine is critical for its efficacy and toxicity. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10-18 2014-05-20T13:24:02Z 2014-05-20T13:24:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5897/AJB10.793 African Journal of Biotechnology. Victoria Island: Academic Journals, v. 9, n. 42, p. 7165-7173, 2010. 1684-5315 http://hdl.handle.net/11449/7371 10.5897/AJB10.793 WOS:000283197400022 WOS000283197400022.pdf 7641979287850489 |
url |
http://dx.doi.org/10.5897/AJB10.793 http://hdl.handle.net/11449/7371 |
identifier_str_mv |
African Journal of Biotechnology. Victoria Island: Academic Journals, v. 9, n. 42, p. 7165-7173, 2010. 1684-5315 10.5897/AJB10.793 WOS:000283197400022 WOS000283197400022.pdf 7641979287850489 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
African Journal of Biotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7165-7173 application/pdf |
dc.publisher.none.fl_str_mv |
Academic Journals |
publisher.none.fl_str_mv |
Academic Journals |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965569494024192 |