Effect of low radiation dose on the expression and location of aquaporins in rat submandibular gland
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.tice.2018.06.006 http://hdl.handle.net/11449/164471 |
Resumo: | Head and neck cancers are common in several regions of the world and the treatment usually includes radiotherapy. This treatment can generate adverse effects to the salivary flow, with a relationship between the dose and the damage caused. Salivary gland cells are highly permeable to water and therefore, they express aquaporins (AQPs). This study analyzed changes in the expression and location of these proteins and identified morphological changes induced by low radiation in rat submandibular gland. Female rats were divided into control and irradiated groups. Immunohistochemistry analysis allowed confirming the presence of AQP1 in the blood vessel endothelium. Intense and steady labelling granules were also observed in the cytoplasm of submandibular gland ductal cells. In addition, there was AQP5 positive labelling in ductal cells delimiting the lumen of intercalated duct, in the cytoplasm and membrane of acinar cells. Finally, the decrease of AQP labelling in irradiated animal glands validated their radiosensitivity. Thus, the decrease in AQP1 protein levels in the endothelium and AQP5 in gland ductal cells of irradiated animals may have hindered the removal of water from the lumen of ductal cells, inducing a delay in water absorption and triggering a slight lumen increase. |
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Repositório Institucional da UNESP |
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Effect of low radiation dose on the expression and location of aquaporins in rat submandibular glandSalivary glandsIrradiationWater channelsHead and neck cancers are common in several regions of the world and the treatment usually includes radiotherapy. This treatment can generate adverse effects to the salivary flow, with a relationship between the dose and the damage caused. Salivary gland cells are highly permeable to water and therefore, they express aquaporins (AQPs). This study analyzed changes in the expression and location of these proteins and identified morphological changes induced by low radiation in rat submandibular gland. Female rats were divided into control and irradiated groups. Immunohistochemistry analysis allowed confirming the presence of AQP1 in the blood vessel endothelium. Intense and steady labelling granules were also observed in the cytoplasm of submandibular gland ductal cells. In addition, there was AQP5 positive labelling in ductal cells delimiting the lumen of intercalated duct, in the cytoplasm and membrane of acinar cells. Finally, the decrease of AQP labelling in irradiated animal glands validated their radiosensitivity. Thus, the decrease in AQP1 protein levels in the endothelium and AQP5 in gland ductal cells of irradiated animals may have hindered the removal of water from the lumen of ductal cells, inducing a delay in water absorption and triggering a slight lumen increase.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fac Med Marilia FAMEMA, Marilia, SP, BrazilUniv Estadual Paulista, Inst Biosci, Dept Anat, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biosci, Dept Anat, Botucatu, SP, BrazilFAPESP: 2013/07203-4Churchill LivingstoneFac Med Marilia FAMEMAUniversidade Estadual Paulista (Unesp)Araujo, M. V. T.Spadella, M. A.Chies, A. B.Arruda, G.Santos, T. de M. [UNESP]Cavariani, M. M. [UNESP]Domeniconi, R. F. [UNESP]2018-11-26T17:54:41Z2018-11-26T17:54:41Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article104-110application/pdfhttp://dx.doi.org/10.1016/j.tice.2018.06.006Tissue & Cell. Edinburgh: Churchill Livingstone, v. 53, p. 104-110, 2018.0040-8166http://hdl.handle.net/11449/16447110.1016/j.tice.2018.06.006WOS:000440173900014WOS000440173900014.pdf54817565282994690000-0003-2938-010XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTissue & Cell0,410info:eu-repo/semantics/openAccess2023-11-26T06:16:47Zoai:repositorio.unesp.br:11449/164471Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:48:39.236256Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of low radiation dose on the expression and location of aquaporins in rat submandibular gland |
title |
Effect of low radiation dose on the expression and location of aquaporins in rat submandibular gland |
spellingShingle |
Effect of low radiation dose on the expression and location of aquaporins in rat submandibular gland Araujo, M. V. T. Salivary glands Irradiation Water channels |
title_short |
Effect of low radiation dose on the expression and location of aquaporins in rat submandibular gland |
title_full |
Effect of low radiation dose on the expression and location of aquaporins in rat submandibular gland |
title_fullStr |
Effect of low radiation dose on the expression and location of aquaporins in rat submandibular gland |
title_full_unstemmed |
Effect of low radiation dose on the expression and location of aquaporins in rat submandibular gland |
title_sort |
Effect of low radiation dose on the expression and location of aquaporins in rat submandibular gland |
author |
Araujo, M. V. T. |
author_facet |
Araujo, M. V. T. Spadella, M. A. Chies, A. B. Arruda, G. Santos, T. de M. [UNESP] Cavariani, M. M. [UNESP] Domeniconi, R. F. [UNESP] |
author_role |
author |
author2 |
Spadella, M. A. Chies, A. B. Arruda, G. Santos, T. de M. [UNESP] Cavariani, M. M. [UNESP] Domeniconi, R. F. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Fac Med Marilia FAMEMA Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Araujo, M. V. T. Spadella, M. A. Chies, A. B. Arruda, G. Santos, T. de M. [UNESP] Cavariani, M. M. [UNESP] Domeniconi, R. F. [UNESP] |
dc.subject.por.fl_str_mv |
Salivary glands Irradiation Water channels |
topic |
Salivary glands Irradiation Water channels |
description |
Head and neck cancers are common in several regions of the world and the treatment usually includes radiotherapy. This treatment can generate adverse effects to the salivary flow, with a relationship between the dose and the damage caused. Salivary gland cells are highly permeable to water and therefore, they express aquaporins (AQPs). This study analyzed changes in the expression and location of these proteins and identified morphological changes induced by low radiation in rat submandibular gland. Female rats were divided into control and irradiated groups. Immunohistochemistry analysis allowed confirming the presence of AQP1 in the blood vessel endothelium. Intense and steady labelling granules were also observed in the cytoplasm of submandibular gland ductal cells. In addition, there was AQP5 positive labelling in ductal cells delimiting the lumen of intercalated duct, in the cytoplasm and membrane of acinar cells. Finally, the decrease of AQP labelling in irradiated animal glands validated their radiosensitivity. Thus, the decrease in AQP1 protein levels in the endothelium and AQP5 in gland ductal cells of irradiated animals may have hindered the removal of water from the lumen of ductal cells, inducing a delay in water absorption and triggering a slight lumen increase. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:54:41Z 2018-11-26T17:54:41Z 2018-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.tice.2018.06.006 Tissue & Cell. Edinburgh: Churchill Livingstone, v. 53, p. 104-110, 2018. 0040-8166 http://hdl.handle.net/11449/164471 10.1016/j.tice.2018.06.006 WOS:000440173900014 WOS000440173900014.pdf 5481756528299469 0000-0003-2938-010X |
url |
http://dx.doi.org/10.1016/j.tice.2018.06.006 http://hdl.handle.net/11449/164471 |
identifier_str_mv |
Tissue & Cell. Edinburgh: Churchill Livingstone, v. 53, p. 104-110, 2018. 0040-8166 10.1016/j.tice.2018.06.006 WOS:000440173900014 WOS000440173900014.pdf 5481756528299469 0000-0003-2938-010X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Tissue & Cell 0,410 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
104-110 application/pdf |
dc.publisher.none.fl_str_mv |
Churchill Livingstone |
publisher.none.fl_str_mv |
Churchill Livingstone |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128983583162368 |