Genetic variability in species of bats revealed by RAPD analysis
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.geneticsmr.com/articles/314 http://www.geneticsmr.com//year2006/vol5-4/pdf/gmr0240.pdf http://hdl.handle.net/11449/33120 |
Resumo: | Random amplified polymorphic DNA molecular marker was utilized as a means of analyzing genetic variability in seven bat species: Molossus molossus, M. rufus, Eumops glaucinus, E. perotis, Myotis nigricans, Eptesicus furinalis, and Artibeus planirostris. The determination of genetic diversity was based on 741 bands produced by a 20-random primer set. Only eight bands were considered monomorphic to one species. The greatest number of bands and the most polymorphic condition were exhibited by M. molossus, followed by M. nigricans, A. planirostris, E. furinalis, E. glaucinus, M. rufus, and E. perotis. Nei's genetic diversity index in the seven species considering the 20 primers was not greater than 0.22, but some primers were capable of detecting values between 0.39 and 0.49. Nei's unbiased genetic distance values and the UPGMA clustering pattern show that M. molossus and M. rufus have a close genetic relationship, unlike that observed between E. perotis and E. glaucinus. The latter was clustered with A. planirostris and E. furinalis. The low values for genetic diversity and distance observed indicate a genetic conservatism in the seven species. The fluorescent in situ hybridization experiments did not confirm a monomorphic condition for the eight bands identified, demonstrating that the monomorphic bands obtained by random amplified polymorphic DNA are insufficient for the identification of bat species. |
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Genetic variability in species of bats revealed by RAPD analysisChiropterafluorescent in situ hybridizationrandom amplified polymorphic DNAgenetic variationBatsRandom amplified polymorphic DNA molecular marker was utilized as a means of analyzing genetic variability in seven bat species: Molossus molossus, M. rufus, Eumops glaucinus, E. perotis, Myotis nigricans, Eptesicus furinalis, and Artibeus planirostris. The determination of genetic diversity was based on 741 bands produced by a 20-random primer set. Only eight bands were considered monomorphic to one species. The greatest number of bands and the most polymorphic condition were exhibited by M. molossus, followed by M. nigricans, A. planirostris, E. furinalis, E. glaucinus, M. rufus, and E. perotis. Nei's genetic diversity index in the seven species considering the 20 primers was not greater than 0.22, but some primers were capable of detecting values between 0.39 and 0.49. Nei's unbiased genetic distance values and the UPGMA clustering pattern show that M. molossus and M. rufus have a close genetic relationship, unlike that observed between E. perotis and E. glaucinus. The latter was clustered with A. planirostris and E. furinalis. The low values for genetic diversity and distance observed indicate a genetic conservatism in the seven species. The fluorescent in situ hybridization experiments did not confirm a monomorphic condition for the eight bands identified, demonstrating that the monomorphic bands obtained by random amplified polymorphic DNA are insufficient for the identification of bat species.UNESP, Inst Biociencias Letras & Ciências Exatas, Dept Zool & Bot, Sao Jose do Rio Preto, SP, BrazilUNESP, Inst Biociencias Letras & Ciências Exatas, Dept Biol, Sao Jose do Rio Preto, SP, BrazilUNESP, Inst Biociencias Letras & Ciências Exatas, Dept Zool & Bot, Sao Jose do Rio Preto, SP, BrazilUNESP, Inst Biociencias Letras & Ciências Exatas, Dept Biol, Sao Jose do Rio Preto, SP, BrazilFunpec-editoraUniversidade Estadual Paulista (Unesp)Moreira, P. R. L. [UNESP]Morielle-Versute, E. [UNESP]2014-05-20T15:22:03Z2014-05-20T15:22:03Z2006-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article804-815application/pdfhttp://www.geneticsmr.com/articles/314http://www.geneticsmr.com//year2006/vol5-4/pdf/gmr0240.pdfGenetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 5, n. 4, p. 804-815, 2006.1676-5680http://hdl.handle.net/11449/33120WOS:0002030120000202-s2.0-39049190315WOS000203012000020.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2023-12-04T06:11:28Zoai:repositorio.unesp.br:11449/33120Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:26:38.586485Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic variability in species of bats revealed by RAPD analysis |
title |
Genetic variability in species of bats revealed by RAPD analysis |
spellingShingle |
Genetic variability in species of bats revealed by RAPD analysis Moreira, P. R. L. [UNESP] Chiroptera fluorescent in situ hybridization random amplified polymorphic DNA genetic variation Bats |
title_short |
Genetic variability in species of bats revealed by RAPD analysis |
title_full |
Genetic variability in species of bats revealed by RAPD analysis |
title_fullStr |
Genetic variability in species of bats revealed by RAPD analysis |
title_full_unstemmed |
Genetic variability in species of bats revealed by RAPD analysis |
title_sort |
Genetic variability in species of bats revealed by RAPD analysis |
author |
Moreira, P. R. L. [UNESP] |
author_facet |
Moreira, P. R. L. [UNESP] Morielle-Versute, E. [UNESP] |
author_role |
author |
author2 |
Morielle-Versute, E. [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Moreira, P. R. L. [UNESP] Morielle-Versute, E. [UNESP] |
dc.subject.por.fl_str_mv |
Chiroptera fluorescent in situ hybridization random amplified polymorphic DNA genetic variation Bats |
topic |
Chiroptera fluorescent in situ hybridization random amplified polymorphic DNA genetic variation Bats |
description |
Random amplified polymorphic DNA molecular marker was utilized as a means of analyzing genetic variability in seven bat species: Molossus molossus, M. rufus, Eumops glaucinus, E. perotis, Myotis nigricans, Eptesicus furinalis, and Artibeus planirostris. The determination of genetic diversity was based on 741 bands produced by a 20-random primer set. Only eight bands were considered monomorphic to one species. The greatest number of bands and the most polymorphic condition were exhibited by M. molossus, followed by M. nigricans, A. planirostris, E. furinalis, E. glaucinus, M. rufus, and E. perotis. Nei's genetic diversity index in the seven species considering the 20 primers was not greater than 0.22, but some primers were capable of detecting values between 0.39 and 0.49. Nei's unbiased genetic distance values and the UPGMA clustering pattern show that M. molossus and M. rufus have a close genetic relationship, unlike that observed between E. perotis and E. glaucinus. The latter was clustered with A. planirostris and E. furinalis. The low values for genetic diversity and distance observed indicate a genetic conservatism in the seven species. The fluorescent in situ hybridization experiments did not confirm a monomorphic condition for the eight bands identified, demonstrating that the monomorphic bands obtained by random amplified polymorphic DNA are insufficient for the identification of bat species. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 2014-05-20T15:22:03Z 2014-05-20T15:22:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.geneticsmr.com/articles/314 http://www.geneticsmr.com//year2006/vol5-4/pdf/gmr0240.pdf Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 5, n. 4, p. 804-815, 2006. 1676-5680 http://hdl.handle.net/11449/33120 WOS:000203012000020 2-s2.0-39049190315 WOS000203012000020.pdf |
url |
http://www.geneticsmr.com/articles/314 http://www.geneticsmr.com//year2006/vol5-4/pdf/gmr0240.pdf http://hdl.handle.net/11449/33120 |
identifier_str_mv |
Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 5, n. 4, p. 804-815, 2006. 1676-5680 WOS:000203012000020 2-s2.0-39049190315 WOS000203012000020.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Research 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
804-815 application/pdf |
dc.publisher.none.fl_str_mv |
Funpec-editora |
publisher.none.fl_str_mv |
Funpec-editora |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129069871529984 |