First isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera: Culicidae): New perspectives for an insect-bacteria association

• Autor(a) principal: Gusmão, Desiely Silva
• Data de Publicação: 2007
• Outros Autores: Santos, Adão Valmir, Marini, Danyelle Cristine, Russo, Érica de Souza, Peixoto, Anelise Maria Dias, Bacci Jr., Maurício [UNESP], Berbert-Molina, Marília Amorim, Lemos, Francisco José Alves
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/S0074-02762007000800005; http://hdl.handle.net/11449/70054
• topic: Aedes aegypti; Gut diverticulum; Microbiota; Serratia sp.; bacterial DNA; ribosome DNA; RNA 16S; RNA 28S; Aedes; animal; Bacillus; gastrointestinal tract; genetics; isolation and purification; microbiology; Pichia; Serratia; Animals; DNA, Bacterial; DNA, Ribosomal; Gastrointestinal Tract; RNA, Ribosomal, 16S; RNA, Ribosomal, 28S; Bacillus (bacterium); Bacillus sp.; Bacillus subtilis; Bacteria (microorganisms); Culicidae; Diptera; Hexapoda; Pichia caribbica

We show for the first time that the ventral diverticulum of the mosquito gut (impermeable sugar storage organ) harbors microorganisms. The gut diverticulum from newly emerged and non-fed Aedes aegypti was dissected under aseptic conditions, homogenized and plated on BHI medium. Microbial isolates were identified by sequencing of 16S rDNA for bacteria and 28S rDNA for yeast. A direct DNA extraction from Ae. aegypti gut diverticulum was also performed. The bacterial isolates were: Bacillus sp., Bacillus subtilis and Serratia sp. The latter was the predominant bacteria found in our isolations. The yeast species identified was Pichia caribbica.