High waterborne Mg does not attenuate the toxic effects of Fe, Mn, and Ba on Na+ regulation of Amazonian armored catfish tamoatá (Hoplosternum litoralle)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11356-018-2064-5 http://hdl.handle.net/11449/176221 |
Resumo: | Formation water (FoW) is a by-product from oil and gas production and usually has high concentrations of soluble salts and metals. Calcium (Ca) and magnesium (Mg) have been shown to reduce the toxicity of metals to aquatic animals, and previous study showed that high waterborne Ca exerts mild effect against disturbances on Na+ regulation in Amazonian armored catfish tamoatá (Hoplosternum littorale) acutely exposed to high Fe, Mn, and Ba levels. Here, we hypothesized that high Mg levels might also reduce the toxic effects of these metals on Na+ regulation of tamoatá. The exposure to 5% FoW promoted an increase in Na+ uptake and a rapid accumulation of Na+ in all tissues analyzed (kidney<plasma<gills<carcass<liver), besides increasing the branchial activity of both NKA and v-type H+-ATPase in fish. High waterborne Mg lowered Na+ efflux rates and markedly inhibited Na+ uptake, and also reduced both NKA activity and newly Na+ accumulation in gills of fish. High Fe levels increased Na+ net losses and inhibited Na+ uptake in tamoatá. The diffusive Na+ losses and the newly accumulated Na+ in gills were reduced in fish exposed to high Mn and Ba. High waterborne Ba also inhibited NKA in gills, while both high Mn and Ba inhibited v-type H+-ATPase in kidney of tamoatá. High Mg did not lessen the toxic effect of Fe on Na+ net fluxes, and reduced even more Na+ uptake and the newly Na+ accumulation in gills and plasma, and did not prevent the inhibition of both NKA and v-type H+-ATPases in kidney. Furthermore, Mg did not attenuate the effect of Mn on inhibition Na+ uptake, keeping the activity of v-type H+-ATPase in kidney significantly lowered. High Mg levels mildly attenuated the effects of Ba in Na+ balance by increasing the new accumulation of Na+ in liver, and restore the activity of both NKA and v-type H+-ATPase in gills of tamoatá. Overall, high waterborne Mg does not have a strong contribution to, or have only minor effects, in protecting tamoatá against disruptions in Na+ regulation mediated by high Fe, Mn, and Ba levels. |
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High waterborne Mg does not attenuate the toxic effects of Fe, Mn, and Ba on Na+ regulation of Amazonian armored catfish tamoatá (Hoplosternum litoralle)Formation waterFreshwater fishMetal toxicityNa+ effluxNa+ uptakeNa+/K+-ATPaseNewly accumulated Na+V-type H+-ATPaseFormation water (FoW) is a by-product from oil and gas production and usually has high concentrations of soluble salts and metals. Calcium (Ca) and magnesium (Mg) have been shown to reduce the toxicity of metals to aquatic animals, and previous study showed that high waterborne Ca exerts mild effect against disturbances on Na+ regulation in Amazonian armored catfish tamoatá (Hoplosternum littorale) acutely exposed to high Fe, Mn, and Ba levels. Here, we hypothesized that high Mg levels might also reduce the toxic effects of these metals on Na+ regulation of tamoatá. The exposure to 5% FoW promoted an increase in Na+ uptake and a rapid accumulation of Na+ in all tissues analyzed (kidney<plasma<gills<carcass<liver), besides increasing the branchial activity of both NKA and v-type H+-ATPase in fish. High waterborne Mg lowered Na+ efflux rates and markedly inhibited Na+ uptake, and also reduced both NKA activity and newly Na+ accumulation in gills of fish. High Fe levels increased Na+ net losses and inhibited Na+ uptake in tamoatá. The diffusive Na+ losses and the newly accumulated Na+ in gills were reduced in fish exposed to high Mn and Ba. High waterborne Ba also inhibited NKA in gills, while both high Mn and Ba inhibited v-type H+-ATPase in kidney of tamoatá. High Mg did not lessen the toxic effect of Fe on Na+ net fluxes, and reduced even more Na+ uptake and the newly Na+ accumulation in gills and plasma, and did not prevent the inhibition of both NKA and v-type H+-ATPases in kidney. Furthermore, Mg did not attenuate the effect of Mn on inhibition Na+ uptake, keeping the activity of v-type H+-ATPase in kidney significantly lowered. High Mg levels mildly attenuated the effects of Ba in Na+ balance by increasing the new accumulation of Na+ in liver, and restore the activity of both NKA and v-type H+-ATPase in gills of tamoatá. Overall, high waterborne Mg does not have a strong contribution to, or have only minor effects, in protecting tamoatá against disruptions in Na+ regulation mediated by high Fe, Mn, and Ba levels.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado do AmazonasConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado do Rio Grande do SulLaboratory of Ecophysiology and Molecular Evolution Brazilian National Institute for Amazonian ResearchSão Paulo State University (UNESP) Institute of Biosciences, Campus do Litoral Paulista, Pça Infante Dom Henrique s/no, Parque BitaruDepartment of Biological Sciences Florida International UniversityInstituto de Oceanografia Laboratório de Aquacultura Continental Universidade Federal do Rio Grande - FURGLaboratório de Ictiologia Aplicada Universidade Vila VelhaDepartamento de Fisiologia e Farmacologia Universidade Federal de Santa MariaSão Paulo State University (UNESP) Institute of Biosciences, Campus do Litoral Paulista, Pça Infante Dom Henrique s/no, Parque BitaruFundação de Amparo à Pesquisa do Estado do Amazonas: 06.03159/2008CNPq: 573976/2008-2Brazilian National Institute for Amazonian ResearchUniversidade Estadual Paulista (Unesp)Florida International UniversityUniversidade Federal do Rio Grande - FURGUniversidade Vila VelhaUniversidade Federal de Santa MariaDuarte, Rafael M. [UNESP]Benaduce, Ana PaulaGarcia, LucianoGomes, Levy C.Gomes, Adriana ChippariVal, Adalberto L.Baldisserotto, Bernardo2018-12-11T17:19:40Z2018-12-11T17:19:40Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article18027-18037application/pdfhttp://dx.doi.org/10.1007/s11356-018-2064-5Environmental Science and Pollution Research, v. 25, n. 18, p. 18027-18037, 2018.1614-74990944-1344http://hdl.handle.net/11449/17622110.1007/s11356-018-2064-52-s2.0-850459444822-s2.0-85045944482.pdf30557957777876120000-0001-5649-0692Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Science and Pollution Research0,858info:eu-repo/semantics/openAccess2023-11-09T06:11:15Zoai:repositorio.unesp.br:11449/176221Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-09T06:11:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
High waterborne Mg does not attenuate the toxic effects of Fe, Mn, and Ba on Na+ regulation of Amazonian armored catfish tamoatá (Hoplosternum litoralle) |
title |
High waterborne Mg does not attenuate the toxic effects of Fe, Mn, and Ba on Na+ regulation of Amazonian armored catfish tamoatá (Hoplosternum litoralle) |
spellingShingle |
High waterborne Mg does not attenuate the toxic effects of Fe, Mn, and Ba on Na+ regulation of Amazonian armored catfish tamoatá (Hoplosternum litoralle) Duarte, Rafael M. [UNESP] Formation water Freshwater fish Metal toxicity Na+ efflux Na+ uptake Na+/K+-ATPase Newly accumulated Na+ V-type H+-ATPase |
title_short |
High waterborne Mg does not attenuate the toxic effects of Fe, Mn, and Ba on Na+ regulation of Amazonian armored catfish tamoatá (Hoplosternum litoralle) |
title_full |
High waterborne Mg does not attenuate the toxic effects of Fe, Mn, and Ba on Na+ regulation of Amazonian armored catfish tamoatá (Hoplosternum litoralle) |
title_fullStr |
High waterborne Mg does not attenuate the toxic effects of Fe, Mn, and Ba on Na+ regulation of Amazonian armored catfish tamoatá (Hoplosternum litoralle) |
title_full_unstemmed |
High waterborne Mg does not attenuate the toxic effects of Fe, Mn, and Ba on Na+ regulation of Amazonian armored catfish tamoatá (Hoplosternum litoralle) |
title_sort |
High waterborne Mg does not attenuate the toxic effects of Fe, Mn, and Ba on Na+ regulation of Amazonian armored catfish tamoatá (Hoplosternum litoralle) |
author |
Duarte, Rafael M. [UNESP] |
author_facet |
Duarte, Rafael M. [UNESP] Benaduce, Ana Paula Garcia, Luciano Gomes, Levy C. Gomes, Adriana Chippari Val, Adalberto L. Baldisserotto, Bernardo |
author_role |
author |
author2 |
Benaduce, Ana Paula Garcia, Luciano Gomes, Levy C. Gomes, Adriana Chippari Val, Adalberto L. Baldisserotto, Bernardo |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Brazilian National Institute for Amazonian Research Universidade Estadual Paulista (Unesp) Florida International University Universidade Federal do Rio Grande - FURG Universidade Vila Velha Universidade Federal de Santa Maria |
dc.contributor.author.fl_str_mv |
Duarte, Rafael M. [UNESP] Benaduce, Ana Paula Garcia, Luciano Gomes, Levy C. Gomes, Adriana Chippari Val, Adalberto L. Baldisserotto, Bernardo |
dc.subject.por.fl_str_mv |
Formation water Freshwater fish Metal toxicity Na+ efflux Na+ uptake Na+/K+-ATPase Newly accumulated Na+ V-type H+-ATPase |
topic |
Formation water Freshwater fish Metal toxicity Na+ efflux Na+ uptake Na+/K+-ATPase Newly accumulated Na+ V-type H+-ATPase |
description |
Formation water (FoW) is a by-product from oil and gas production and usually has high concentrations of soluble salts and metals. Calcium (Ca) and magnesium (Mg) have been shown to reduce the toxicity of metals to aquatic animals, and previous study showed that high waterborne Ca exerts mild effect against disturbances on Na+ regulation in Amazonian armored catfish tamoatá (Hoplosternum littorale) acutely exposed to high Fe, Mn, and Ba levels. Here, we hypothesized that high Mg levels might also reduce the toxic effects of these metals on Na+ regulation of tamoatá. The exposure to 5% FoW promoted an increase in Na+ uptake and a rapid accumulation of Na+ in all tissues analyzed (kidney<plasma<gills<carcass<liver), besides increasing the branchial activity of both NKA and v-type H+-ATPase in fish. High waterborne Mg lowered Na+ efflux rates and markedly inhibited Na+ uptake, and also reduced both NKA activity and newly Na+ accumulation in gills of fish. High Fe levels increased Na+ net losses and inhibited Na+ uptake in tamoatá. The diffusive Na+ losses and the newly accumulated Na+ in gills were reduced in fish exposed to high Mn and Ba. High waterborne Ba also inhibited NKA in gills, while both high Mn and Ba inhibited v-type H+-ATPase in kidney of tamoatá. High Mg did not lessen the toxic effect of Fe on Na+ net fluxes, and reduced even more Na+ uptake and the newly Na+ accumulation in gills and plasma, and did not prevent the inhibition of both NKA and v-type H+-ATPases in kidney. Furthermore, Mg did not attenuate the effect of Mn on inhibition Na+ uptake, keeping the activity of v-type H+-ATPase in kidney significantly lowered. High Mg levels mildly attenuated the effects of Ba in Na+ balance by increasing the new accumulation of Na+ in liver, and restore the activity of both NKA and v-type H+-ATPase in gills of tamoatá. Overall, high waterborne Mg does not have a strong contribution to, or have only minor effects, in protecting tamoatá against disruptions in Na+ regulation mediated by high Fe, Mn, and Ba levels. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:19:40Z 2018-12-11T17:19:40Z 2018-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11356-018-2064-5 Environmental Science and Pollution Research, v. 25, n. 18, p. 18027-18037, 2018. 1614-7499 0944-1344 http://hdl.handle.net/11449/176221 10.1007/s11356-018-2064-5 2-s2.0-85045944482 2-s2.0-85045944482.pdf 3055795777787612 0000-0001-5649-0692 |
url |
http://dx.doi.org/10.1007/s11356-018-2064-5 http://hdl.handle.net/11449/176221 |
identifier_str_mv |
Environmental Science and Pollution Research, v. 25, n. 18, p. 18027-18037, 2018. 1614-7499 0944-1344 10.1007/s11356-018-2064-5 2-s2.0-85045944482 2-s2.0-85045944482.pdf 3055795777787612 0000-0001-5649-0692 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Science and Pollution Research 0,858 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
18027-18037 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964872577908736 |