Investigation on the s of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa

Detalhes bibliográficos
Autor(a) principal: Luber, Jaquelini
Data de Publicação: 2015
Outros Autores: Palmieri, Marcel J., Botelho, Carolina M., Rinaldo, Daniel [UNESP], Andrade-Vieira, Larissa F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200903&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/127503
Resumo: Guava (Psidium guajava L.) is a plant often employed in popular medicine. Recently several studies have alerted about the toxicity of substances present in medicinal plants, which can pose risks to the human health. In this sense, the present work aimed to investigate the phytotoxic, cytotoxic and genotoxic action of three guava varieties - Paluma, Pedro Sato and Roxa (purple) - on the plant test system Lactuca sativa L. Thus, macro- and microscopic evaluations were carried out for five infusion concentrations (2.5, 5.0, 10.0, 20.0 and 40.0 g.L-1) prepared from each variety. Distilled water was used as negative control. Chromatographic and spectroscopic analysis by HPLC-PAD indicated that the chemical composition of the infusion of Roxa is different than that of the infusions of the varieties Paluma and Pedro Sato. It was observed that seed germination and root growth in L. sativa exposed to infusions decreased with increasing infusion concentration, regardless of the tested cultivar. For the mitotic index, no statistical differences were observed. On the other hand, a significant increase in the frequency of cell cycle alterations was verified, especially for the highest concentrations tested. The cytogenotoxic was significant. Therefore, guava should not be used indiscriminately in popular medicine.
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spelling Investigation on the s of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativaCitogenetoxicityRoot growthMitotic indexSeed germinationIC50CitogenotoxicidadeCrescimento radicularÍndice mitóticoGerminação de sementesIC50Guava (Psidium guajava L.) is a plant often employed in popular medicine. Recently several studies have alerted about the toxicity of substances present in medicinal plants, which can pose risks to the human health. In this sense, the present work aimed to investigate the phytotoxic, cytotoxic and genotoxic action of three guava varieties - Paluma, Pedro Sato and Roxa (purple) - on the plant test system Lactuca sativa L. Thus, macro- and microscopic evaluations were carried out for five infusion concentrations (2.5, 5.0, 10.0, 20.0 and 40.0 g.L-1) prepared from each variety. Distilled water was used as negative control. Chromatographic and spectroscopic analysis by HPLC-PAD indicated that the chemical composition of the infusion of Roxa is different than that of the infusions of the varieties Paluma and Pedro Sato. It was observed that seed germination and root growth in L. sativa exposed to infusions decreased with increasing infusion concentration, regardless of the tested cultivar. For the mitotic index, no statistical differences were observed. On the other hand, a significant increase in the frequency of cell cycle alterations was verified, especially for the highest concentrations tested. The cytogenotoxic was significant. Therefore, guava should not be used indiscriminately in popular medicine.A goiaba (Psidium guajava L.) é uma planta bastante utilizada na medicina popular. Recentemente alguns trabalhos tem alertado acerca da toxicidade de substâncias presentes em plantas medicinais, o que pode trazer riscos à saúde humana. Neste sentido, o presente trabalho objetivou investigar a ação fitotóxica, citotóxica e genotóxica de três variedades de goiaba - Paluma, Pedro Sato e Roxa - no sistema teste vegetal Lactuca sativa L. Assim, foram realizadas avaliações macro- e microscópicas para cinco concentrações de infusões (2,5, 5,0, 10,0, 20,0 e 40,0 g.L-1) preparadas a partir de cada variedade. A água destilada foi usada como controle negativo. As análises cromatográficas e espectroscópicas por HPLC-PAD indicaram que a composição química da infusão da Roxa é diferente das infusões das variedades Paluma e Pedro Sato. Foi observado que a germinação das sementes e o crescimento da raiz em L. sativa expostas às infusões diminuem com o aumento da concentração da infusão, independentemente da cultivar testada. Para o índice mitótico, diferenças estatísticas não foram observadas. Por outro lado, foi verificado um aumento significativo na frequência de alterações do ciclo celular, especialmente para as maiores concentrações testadas. O efeito citogenotóxico foi significativo. Portanto, a goiaba não deve ser utilizada indiscriminadamente na medicina popular.Universidade Federal do Espírito Santo Centro de Ciências Agrárias Departamento de BiologiaUniversidade Federal de Lavras Departamento de BiologiaUniversidade Estadual Paulista Faculdade de Ciências Departamento de QuímicaUniversidade Estadual Paulista Faculdade de Ciências Departamento de QuímicaAcademia Brasileira de CiênciasUniversidade Federal do Espírito Santo (UFES)Universidade Federal de Lavras (UFLA)Universidade Estadual Paulista (Unesp)Luber, JaqueliniPalmieri, Marcel J.Botelho, Carolina M.Rinaldo, Daniel [UNESP]Andrade-Vieira, Larissa F.2015-08-26T19:22:35Z2015-08-26T19:22:35Z2015-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article903-913application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200903&lng=en&nrm=iso&tlng=enAnais da Academia Brasileira de Ciências, v. 87, n. 2, p. 903-913, 2015.0001-3765http://hdl.handle.net/11449/12750310.1590/0001-3765201520140286S0001-37652015000200903S0001-37652015000200903.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnais da Academia Brasileira de Ciências0.9560,418info:eu-repo/semantics/openAccess2024-04-29T18:16:48Zoai:repositorio.unesp.br:11449/127503Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:40:55.921758Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Investigation on the s of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa
title Investigation on the s of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa
spellingShingle Investigation on the s of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa
Luber, Jaquelini
Citogenetoxicity
Root growth
Mitotic index
Seed germination
IC50
Citogenotoxicidade
Crescimento radicular
Índice mitótico
Germinação de sementes
IC50
title_short Investigation on the s of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa
title_full Investigation on the s of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa
title_fullStr Investigation on the s of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa
title_full_unstemmed Investigation on the s of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa
title_sort Investigation on the s of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa
author Luber, Jaquelini
author_facet Luber, Jaquelini
Palmieri, Marcel J.
Botelho, Carolina M.
Rinaldo, Daniel [UNESP]
Andrade-Vieira, Larissa F.
author_role author
author2 Palmieri, Marcel J.
Botelho, Carolina M.
Rinaldo, Daniel [UNESP]
Andrade-Vieira, Larissa F.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do Espírito Santo (UFES)
Universidade Federal de Lavras (UFLA)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Luber, Jaquelini
Palmieri, Marcel J.
Botelho, Carolina M.
Rinaldo, Daniel [UNESP]
Andrade-Vieira, Larissa F.
dc.subject.por.fl_str_mv Citogenetoxicity
Root growth
Mitotic index
Seed germination
IC50
Citogenotoxicidade
Crescimento radicular
Índice mitótico
Germinação de sementes
IC50
topic Citogenetoxicity
Root growth
Mitotic index
Seed germination
IC50
Citogenotoxicidade
Crescimento radicular
Índice mitótico
Germinação de sementes
IC50
description Guava (Psidium guajava L.) is a plant often employed in popular medicine. Recently several studies have alerted about the toxicity of substances present in medicinal plants, which can pose risks to the human health. In this sense, the present work aimed to investigate the phytotoxic, cytotoxic and genotoxic action of three guava varieties - Paluma, Pedro Sato and Roxa (purple) - on the plant test system Lactuca sativa L. Thus, macro- and microscopic evaluations were carried out for five infusion concentrations (2.5, 5.0, 10.0, 20.0 and 40.0 g.L-1) prepared from each variety. Distilled water was used as negative control. Chromatographic and spectroscopic analysis by HPLC-PAD indicated that the chemical composition of the infusion of Roxa is different than that of the infusions of the varieties Paluma and Pedro Sato. It was observed that seed germination and root growth in L. sativa exposed to infusions decreased with increasing infusion concentration, regardless of the tested cultivar. For the mitotic index, no statistical differences were observed. On the other hand, a significant increase in the frequency of cell cycle alterations was verified, especially for the highest concentrations tested. The cytogenotoxic was significant. Therefore, guava should not be used indiscriminately in popular medicine.
publishDate 2015
dc.date.none.fl_str_mv 2015-08-26T19:22:35Z
2015-08-26T19:22:35Z
2015-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200903&lng=en&nrm=iso&tlng=en
Anais da Academia Brasileira de Ciências, v. 87, n. 2, p. 903-913, 2015.
0001-3765
http://hdl.handle.net/11449/127503
10.1590/0001-3765201520140286
S0001-37652015000200903
S0001-37652015000200903.pdf
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200903&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/127503
identifier_str_mv Anais da Academia Brasileira de Ciências, v. 87, n. 2, p. 903-913, 2015.
0001-3765
10.1590/0001-3765201520140286
S0001-37652015000200903
S0001-37652015000200903.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Anais da Academia Brasileira de Ciências
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 903-913
application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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